Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005477

Physical Location & Seq

Genomic Coordinates Reverse (-)

20016038 .. 20023799

Gene Structure 7762 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g24570.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3357 bp

ATGGAGGAACCAGCCCCCGCCGGCCGCCCGAAGCGGACTAGGGCTCAGTCTCGATTCACTACCGAGAACAACAACGGTGGCGATGCCAGTGAACACGTGGACCGCGAGAGCTCGCCGGACGATTTCGAAGCGCCTCGTCCTAAAGCTAAACGAGGTCGCCCTCCCAGTGGCCCCGCCGCCGCGCCGCCCAAAGCCTCCGCTCTCACTTTGATCGAGGTCGTCAAGGGAAACGGGAAACTTATTCCACAAGCGGTCAAGCTCTGGGTGGAGCGGTACGAAAAGGATGCTAAACCTGCTACTGTTGAACTCTTGACAATGCTGTTTGAGGCATGCGGAGCTAAGTATCATATCAAAGAAGAGTTGCTGGACGAGACTAATGTTGATGATGTTGTGGTTGCACTTGTGGAGCTTGCTAGAAATGGTAACGTTGATGATTACCAAAGTTCGAAAAAGGAGTTCAAAAACATAAAAGACAATCTCCAATCATTCTGGGATAAGTTGGTCTGCGAATGTCAACACGGGCCATTGTTTGATCAGATTTTGTTCGAGAAGTGTATGGGATATATAATTGCTCTATCATGCACTCCTCCAAGAGTTTATCGGCAAACTGCTACTTTGATGGGTCTCCAACTTGTCACTTCGTTCATAACTGTTGCTAAAACACTTGGTCTTCAGCGTGAAACTACTCGAAGACAATTAGAGGCTGAGAAAAAGAAACAATCTGAAGGACCTCGTGTGGAGTCACTGAACAAGAGGTTCTCGACGACTCATGAAAACATAACTCTTCTAGAGCAGATGATGCGGAATATATTTCAAGGGTTATTTGTGCATCGCTACCGAGACATTGAACCAAATATTCGGACGTCTTGTATAGAGGCACTCGGCGTGTGGATTTTGTCATATCCATCAATGTTCTTGCAGGATTTATACTTGAAATATCTTGGATGGACATTGAATGACAAAAATGCTGGTGTAAGAAAAGCATCAGTCCTTGCATTACAAAATCTTTATGAGGTTGATGATAATGTGCCAACTCTGGGCCTATTCACCGAAAGATTTTCTACTCGGATGATTGAGCTTGCTGATGACATAGATATTTCTGTGGCTGTTTGTGCCATTGGCCTTGTTAAACAGCTTTTAAGACATCAGCTTCTACCAGATGATGACTTGGGTCCTTTATATGATTTGCTTATTGATGATCCAGCAGAGATCAGGCATGCTATAGGAGCATTAGTGTATGAACACTTGATTTCTCAAAAGTTCATTAGCTCCCAGTCTGGTGCAAAAGGTGTTGACAATAATTCTTCTGAGGTCCAGCTCGGCAGAATGTTGCAGATACTAAGAGAGTTCTCTGCAGATCCAATTCTTAGCGTTTATGTAATTGATGATGTCTGGGAATACATGAATGCCATGAAGGATTGGAAGTGTATTATCTCCATGCTCTTGGACGAGAATCCATTGATTGAGCTTACTGATGAGGATGCAACAAACTTGGTACGACTTCTTTGTGCATCTGTCAAAAAGGCTGTAGGAGAGAGGATAGTTCCTGCTACTGATAATCGAAAGCCATACTACACTAAAGCTCAAAAAGATGTATTTGAGCACAACAAACAGGATATAACTCTTGCCATGATGAAGAATTATCCACTACTTCTCCGTAAGTTCATGGCAGACAAAGCAAAAATACCATCACTGGTTGACATTATTCTACACATGAATCTTGGGCTATATTCCAAAAGGAGGCAGGAACAGAATTTTGAAACCGTCATTCAACTGATCAAAGAGGCATTTTTCAAACATGGTGAGAAGGAGGCACTGAGATCTTGTGCTAATGCTATCATGTTTTGTTCAACGGACAGTCAAGGGGAGCTAAAAGATACTGCCCGCAATTTAGTAAAGGAACTTCATGATGAGCTTATTCATAGTAAGCTTAAACCTGCGATGAAAGAAGTGGCGGATGGTGGTGATGAATATCCTCTTCTTGTAAATTTGAAAAGAATGTATGAGCTTCAATTGTCAGGAAATGTGCCTATTGAAAGTTTGTATGAAGATATGGTCTCAACTCTTCAGAGCTATACAAACAAGGATGATCAGGTTGTCACTTTTCTGCTTCTCAACATGTATATGCATGTGCAATGGTGTCTGCATGCCATCATACATAGTCCTGCTATCTCAGAGGCATCTTTATCTTCCCTATTAGCGAAACGTGATTTTTTGTCAGAGCGGTTAGAGTACCTCCTTAGCAGCTGTCTGGAAATGGAGGGTAATCGTTGCAATCAACTAGCTCGCTGGGTCTGTGTTATACTCACTGATTTGTCTAGACTGTTCAAAAAGACAAACTTTTCTTCATCGAAGCTGGAAAAATTAGGATATTCTCCAGATGCATCAGTTCTTCAAAAGTTCTGGAAACTTTCTGTGCAACAGCTCAGTATTTCAGATGACACGGAAGATGACGATGGGAACAAAGAGTATATTGAGGAAACAAATAGAGATACTGTCATGATGGCTGCTGTACGGTTAGCTGTTACTGATAAAGTTTATAAGGAGTACCTGGGTCCAGAGATTATATCTCGATTCATGATGCATGGTACAACTGTAGCCGAGATAGTTAAGCATCTGATAACTTTCCTGAAGAAGAATGATGATGATCTTGCGAAAACTTTCTTGGAAGCGCTGAAAATGGCCTACCATCGGTATATGGTTGAACTATCTAGAAGTGATGATGAATCATTAGCAAGCAAGCATTTTCTAGAGTTTCAAGAACTAGCTGCTCAGCTTTCTGGGACGTTTGTAGGTGTTTCCAAGAACAAGCACAAGTCAGACATTTTGAAAATTGTTAACCAGGGTATTGACTATGCTTTTATAGACACTCCAAAGCAGCTGTCCTTTCTGGAAGGTGCTGTGCTCCAATTTGTGTCCAAATTGCCTACACCTGATATTCTTGAGATTAATGTCAATATCCAAAAGAGAACGGAGAATGTTAATATGGATGAAGATCCTAGTGGCTGGCGTCCATATCACACATTCGTTCAGACCTTGCGTGAAAAATTTGCAAAGATTGAAGGTATGCAAGAAGAGAAGGACGGGACCTCTGTTAGGCGCAGGGGTCGTCCACGGAAAAGGCGTAATATACAGGGAAAGAGGCTGTTTGATGATCATGGTTCAAGTGAAGAAGAGGATTCAATTAGTGGATCTGACCATGAAAATGCTCAAGATGAAGAAGAACAACAAGATGAGGATGAAGACGATGATGATGATGAAGATGAAGATGTTCCTTTAATAAATCAAATTAGGTCATCATCCAAGTTAAGATCGAGAGGTTCACGAGAGGGAAACAAAGGCCAGACACAAACGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007127 GO:0007135 GO:0008150 GO:0008608 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0045132 GO:0045143 GO:0045144 GO:0048285 GO:0051177 GO:0051276 GO:0051316 GO:0051321 GO:0051455 GO:0051754 GO:0061982 GO:0061983 GO:0070192 GO:0070601 GO:0071840 GO:0098813 GO:0140013 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

STAG

Protein Analysis

1119

Amino Acids

127.33

Weight (kDa)

5.39

Isoelectric Point (pI)

51.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STAG	PF08514	94 - 198	6.2e-27	STAG domain
SCD	PF21581	227 - 300	1.4e-29	Stromalin conservative domain
HEAT_SCC3-SA	PF24571	406 - 660	1.8e-70	Cohesin subunit SCC3/SA, HEAT-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47980
fragaria_vesca	FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570
malus_domestica	MD11G1296700.v1.1
prunus_persica	Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1
pyrus_communis	pycom11g26100
rosa_chinensis	RchiOBHm_Chr6g0292391
rosa_laevigata	RLG00000012032
rosa_multiflora	Rmu_sc0012226.1_g000001 Rmu_sc0023540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026580 Rroxscaffold_7G00175100
rosa_rugosa	Rorug06G0226100 Rorug06G0226200 Rorug06G0226300
rosa_samantha	Rh6AG339600 Rh6BG346800 Rh6CG353600 Rh6DG339600
rosa_wichuraiana	Rw6G029550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2541
AatII	GACGTC	1 cut(s)	866
Acc36I	ACCTGC	2 cut(s)	301, 1945
AccBSI	CCGCTC	3 cut(s)	200, 271, 2224
AccII	CGCG	2 cut(s)	105, 182
AclI	AACGTT	1 cut(s)	426
AclWI	GGATC	4 cut(s)	1193, 1352, 2990, 3199
AcoI	YGGCCR	1 cut(s)	22
AcsI	RAATTY	3 cut(s)	1753, 1987, 3047
AcuI	CTGAAG	4 cut(s)	656, 744, 2051, 2651
AcvI	CACGTG	1 cut(s)	97
AcyI	GRCGYC	2 cut(s)	863, 3010
AfaI	GTAC	6 cut(s)	275, 1497, 2234, 2514, 2549, 2590
AfeI	AGCGCT	1 cut(s)	2673
AfiI	CCNNNNNNNGG	5 cut(s)	167, 1037, 1740, 2691, 3096
AflIII	ACRYGT	2 cut(s)	94, 2118
AjnI	CCWGG	2 cut(s)	2550, 2841
AjuI	GAANNNNNNNTTGG	2 cut(s)	2648, 2680
AleI	CACNNNNGTG	1 cut(s)	3352
Alw21I	GWGCWC	3 cut(s)	113, 1605, 2907
Alw26I	GTCTC	5 cut(s)	54, 365, 629, 834, 2062
AlwI	GGATC	4 cut(s)	1193, 1352, 2990, 3199
Aor51HI	AGCGCT	1 cut(s)	2673
AoxI	GGCC	7 cut(s)	22, 169, 521, 1039, 1120, 2682, 3340
ApeKI	GCWGC	4 cut(s)	2244, 2507, 2768, 2878
ApoI	RAATTY	3 cut(s)	1753, 1987, 3047
ArsI	GACNNNNNNTTYG	2 cut(s)	2867, 2899
AseI	ATTAAT	1 cut(s)	2949
Asp700I	GAANNNNTTC	2 cut(s)	2660, 3270
AspLEI	GCGC	4 cut(s)	133, 184, 2674, 3102
AspS9I	GGNCC	9 cut(s)	100, 170, 521, 728, 1039, 1172, 1312, 2555, 3087
AsuHPI	GGTGA	3 cut(s)	1039, 1814, 1976
AsuII	TTCGAA	2 cut(s)	126, 446
AvaII	GGWCC	6 cut(s)	100, 728, 1172, 1312, 2555, 3087
BaeI	ACNNNNGTAYC	2 cut(s)	2580, 2613
BanII	GRGCYC	2 cut(s)	46, 113
BarI	GAAGNNNNNNTAC	2 cut(s)	1887, 1919
BauI	CACGAG	2 cut(s)	732, 3324
BbrPI	CACGTG	1 cut(s)	97
BbsI	GAAGAC	3 cut(s)	662, 697, 3249
Bbv12I	GWGCWC	3 cut(s)	113, 1605, 2907
BbvI	GCAGC	4 cut(s)	2256, 2494, 2755, 2890
BccI	CCATC	9 cut(s)	613, 913, 939, 1696, 1952, 2159, 2450, 2497, 2697
BcgI	CGANNNNNNTGC	2 cut(s)	266, 300
BciT130I	CCWGG	2 cut(s)	2552, 2843
BclI	TGATCA	4 cut(s)	532, 1776, 2089, 3154
BcoDI	GTCTC	5 cut(s)	54, 365, 629, 834, 2062
BfaI	CTAG	9 cut(s)	39, 414, 788, 2282, 2319, 2712, 2750, 2765, 3000
BfmI	CTRYAG	4 cut(s)	1221, 1353, 1527, 2595
BfoI	RGCGCY	2 cut(s)	134, 2675
BfuAI	ACCTGC	2 cut(s)	301, 1945
BglII	AGATCT	1 cut(s)	1820
BisI	GCNGC	8 cut(s)	25, 177, 180, 185, 2245, 2508, 2769, 2879
BlpI	GCTNAGC	1 cut(s)	2772
BlsI	GCNGC	8 cut(s)	26, 178, 181, 186, 2246, 2509, 2770, 2880
Bme1390I	CCNGG	2 cut(s)	2552, 2843
Bme18I	GGWCC	6 cut(s)	100, 728, 1172, 1312, 2555, 3087
BmgT120I	GGNCC	9 cut(s)	100, 170, 521, 728, 1039, 1172, 1312, 2555, 3087
BmiI	GGNNCC	5 cut(s)	9, 172, 1173, 2556, 3088
BmrFI	CCNGG	2 cut(s)	2552, 2843
BmrI	ACTGGG	2 cut(s)	159, 1267
BmuI	ACTGGG	2 cut(s)	159, 1267
BoxI	GACNNNNGTC	1 cut(s)	1170
BpiI	GAAGAC	3 cut(s)	662, 697, 3249
BpmI	CTGGAG	1 cut(s)	2361
Bpu10I	CCTNAGC	1 cut(s)	2240
Bpu1102I	GCTNAGC	1 cut(s)	2772
Bpu14I	TTCGAA	2 cut(s)	126, 446
BpuEI	CTTGAG	2 cut(s)	2963, 3195
BsaAI	YACGTR	1 cut(s)	97
BsaBI	GATNNNNATC	3 cut(s)	1971, 2646, 2994
BsaHI	GRCGYC	2 cut(s)	863, 3010
BsaI	GGTCTC	2 cut(s)	629, 2062
BsaJI	CCNNGG	3 cut(s)	2551, 2842, 3113
BsaXI	ACNNNNNCTCC	4 cut(s)	1218, 1248, 1638, 1668
Bsc4I	CCNNNNNNNGG	5 cut(s)	167, 1037, 1740, 2691, 3096
Bse118I	RCCGGY	1 cut(s)	20
Bse1I	ACTGG	4 cut(s)	87, 165, 1273, 1698
Bse3DI	GCAATG	1 cut(s)	2141
Bse8I	GATNNNNATC	3 cut(s)	1971, 2646, 2994
BseBI	CCWGG	2 cut(s)	2552, 2843
BseDI	CCNNGG	3 cut(s)	2551, 2842, 3113
BseGI	GGATG	9 cut(s)	289, 950, 1074, 1486, 1963, 2092, 2995, 3244, 3299
BseJI	GATNNNNATC	3 cut(s)	1971, 2646, 2994
BseLI	CCNNNNNNNGG	5 cut(s)	167, 1037, 1740, 2691, 3096
BseMI	GCAATG	1 cut(s)	2141
BseMII	CTCAG	7 cut(s)	59, 696, 1299, 1808, 2187, 2440, 2786
BseNI	ACTGG	4 cut(s)	87, 165, 1273, 1698
BseRI	GAGGAG	1 cut(s)	576
BseX3I	CGGCCG	1 cut(s)	22
BseXI	GCAGC	4 cut(s)	2256, 2494, 2755, 2890
BseYI	CCCAGC	1 cut(s)	2289
Bsh1236I	CGCG	2 cut(s)	105, 182
Bsh1285I	CGRYCG	1 cut(s)	25
BshFI	GGCC	7 cut(s)	24, 171, 523, 1041, 1122, 2684, 3342
BsiEI	CGRYCG	1 cut(s)	25
BsiHKAI	GWGCWC	3 cut(s)	113, 1605, 2907
BsiSI	CCGG	2 cut(s)	21, 116
BslFI	GGGAC	2 cut(s)	2797, 3100
BslI	CCNNNNNNNGG	5 cut(s)	167, 1037, 1740, 2691, 3096
BsmAI	GTCTC	5 cut(s)	54, 365, 629, 834, 2062
BsmFI	GGGAC	2 cut(s)	2797, 3100
BsmI	GAATGC	1 cut(s)	1411
BsnI	GGCC	7 cut(s)	24, 171, 523, 1041, 1122, 2684, 3342
Bso31I	GGTCTC	2 cut(s)	629, 2062
Bsp119I	TTCGAA	2 cut(s)	126, 446
Bsp1286I	GDGCHC	4 cut(s)	46, 113, 1605, 2907
Bsp1720I	GCTNAGC	1 cut(s)	2772
BspANI	GGCC	7 cut(s)	24, 171, 523, 1041, 1122, 2684, 3342
BspCNI	CTCAG	7 cut(s)	58, 697, 1300, 1809, 2186, 2439, 2785
BspFNI	CGCG	2 cut(s)	105, 182
BspHI	TCATGA	4 cut(s)	769, 1906, 2499, 2577
BspLI	GGNNCC	5 cut(s)	9, 172, 1173, 2556, 3088
BspMAI	CTGCAG	1 cut(s)	1357
BspMI	ACCTGC	2 cut(s)	301, 1945
BspPI	GGATC	4 cut(s)	1193, 1352, 2990, 3199
BspT104I	TTCGAA	2 cut(s)	126, 446
BspTNI	GGTCTC	2 cut(s)	629, 2062
BsrBI	CCGCTC	3 cut(s)	200, 271, 2224
BsrDI	GCAATG	1 cut(s)	2141
BsrFI	RCCGGY	1 cut(s)	20
BsrI	ACTGG	4 cut(s)	87, 165, 1273, 1698
BssAI	RCCGGY	1 cut(s)	20
BssECI	CCNNGG	3 cut(s)	2551, 2842, 3113
BssNI	GRCGYC	2 cut(s)	863, 3010
BssSI	CACGAG	2 cut(s)	732, 3324
Bst2BI	CACGAG	2 cut(s)	732, 3324
Bst2UI	CCWGG	2 cut(s)	2552, 2843
Bst4CI	ACNGT	9 cut(s)	77, 301, 652, 1765, 1859, 2325, 2497, 2517, 2596
Bst6I	CTCTTC	6 cut(s)	351, 789, 1983, 2070, 3069, 3168
BstACI	GRCGYC	2 cut(s)	863, 3010
BstAPI	GCANNNNNTGC	1 cut(s)	799
BstBAI	YACGTR	1 cut(s)	97
BstBI	TTCGAA	2 cut(s)	126, 446
BstDSI	CCRYGG	1 cut(s)	3113
BstENI	CCTNNNNNAGG	1 cut(s)	3094
BstF5I	GGATG	9 cut(s)	289, 950, 1074, 1486, 1963, 2092, 2995, 3244, 3299
BstFNI	CGCG	2 cut(s)	105, 182
BstH2I	RGCGCY	2 cut(s)	134, 2675
BstHHI	GCGC	4 cut(s)	133, 184, 2674, 3102
BstMAI	GTCTC	5 cut(s)	54, 365, 629, 834, 2062
BstMCI	CGRYCG	1 cut(s)	25
BstMWI	GCNNNNNNNGC	6 cut(s)	293, 335, 799, 1112, 1226, 1676
BstNI	CCWGG	2 cut(s)	2552, 2843
BstNSI	RCATGY	5 cut(s)	333, 1220, 2122, 2132, 2150
BstPAI	GACNNNNGTC	1 cut(s)	1170
BstSCI	CCNGG	2 cut(s)	2550, 2841
BstSFI	CTRYAG	4 cut(s)	1221, 1353, 1527, 2595
BstUI	CGCG	2 cut(s)	105, 182
BstV1I	GCAGC	4 cut(s)	2256, 2494, 2755, 2890
BstV2I	GAAGAC	3 cut(s)	662, 697, 3249
BstX2I	RGATCY	4 cut(s)	1357, 1820, 2995, 3191
BstXI	CCANNNNNNTGG	1 cut(s)	1444
BstYI	RGATCY	4 cut(s)	1357, 1820, 2995, 3191
BstZI	CGGCCG	1 cut(s)	22
BsuRI	GGCC	7 cut(s)	24, 171, 523, 1041, 1122, 2684, 3342
BtgI	CCRYGG	1 cut(s)	3113
BtgZI	GCGATG	3 cut(s)	96, 815, 1955
BtsCI	GGATG	9 cut(s)	289, 950, 1074, 1486, 1963, 2092, 2995, 3244, 3299
BtsIMutI	CAGTG	6 cut(s)	94, 172, 743, 1691, 1814, 2307
BveI	ACCTGC	2 cut(s)	301, 1945
CciI	TCATGA	4 cut(s)	769, 1906, 2499, 2577
CfoI	GCGC	4 cut(s)	133, 184, 2674, 3102
Cfr10I	RCCGGY	1 cut(s)	20
Cfr13I	GGNCC	9 cut(s)	100, 170, 521, 728, 1039, 1172, 1312, 2555, 3087
CseI	GACGC	1 cut(s)	2999
Csp6I	GTAC	6 cut(s)	274, 1496, 2233, 2513, 2548, 2589
CspCI	CAANNNNNGTGG	2 cut(s)	58, 93
CviQI	GTAC	6 cut(s)	274, 1496, 2233, 2513, 2548, 2589
EaeI	YGGCCR	1 cut(s)	22
EagI	CGGCCG	1 cut(s)	22
Eam1104I	CTCTTC	6 cut(s)	351, 789, 1983, 2070, 3069, 3168
EarI	CTCTTC	6 cut(s)	351, 789, 1983, 2070, 3069, 3168
EciI	GGCGGA	1 cut(s)	1970
Ecl136II	GAGCTC	1 cut(s)	111
EclXI	CGGCCG	1 cut(s)	22
Eco24I	GRGCYC	2 cut(s)	46, 113
Eco31I	GGTCTC	2 cut(s)	629, 2062
Eco47I	GGWCC	6 cut(s)	100, 728, 1172, 1312, 2555, 3087
Eco47III	AGCGCT	1 cut(s)	2673
Eco52I	CGGCCG	1 cut(s)	22
Eco53kI	GAGCTC	1 cut(s)	111
Eco57I	CTGAAG	4 cut(s)	656, 744, 2051, 2651
Eco72I	CACGTG	1 cut(s)	97
EcoICRI	GAGCTC	1 cut(s)	111
EcoNI	CCTNNNNNAGG	1 cut(s)	3094
EcoO109I	RGGNCCY	3 cut(s)	728, 1172, 3087
EcoRII	CCWGG	2 cut(s)	2550, 2841
EcoT22I	ATGCAT	3 cut(s)	2130, 2386, 2586
EcoT38I	GRGCYC	2 cut(s)	46, 113
FalI	AAGNNNNNCTT	2 cut(s)	2324, 2356
FaqI	GGGAC	2 cut(s)	2797, 3100
FauI	CCCGC	3 cut(s)	25, 181, 1892
FbaI	TGATCA	4 cut(s)	532, 1776, 2089, 3154
Fnu4HI	GCNGC	8 cut(s)	25, 177, 180, 185, 2245, 2508, 2769, 2879
FokI	GGATG	9 cut(s)	296, 957, 1081, 1493, 1970, 2099, 3002, 3251, 3286
FriOI	GRGCYC	2 cut(s)	46, 113
Fsp4HI	GCNGC	8 cut(s)	25, 177, 180, 185, 2245, 2508, 2769, 2879
FspBI	CTAG	9 cut(s)	39, 414, 788, 2282, 2319, 2712, 2750, 2765, 3000
GlaI	GCGC	4 cut(s)	132, 183, 2673, 3101
GluI	GCNGC	8 cut(s)	25, 177, 180, 185, 2245, 2508, 2769, 2879
GsaI	CCCAGC	1 cut(s)	2293
GsuI	CTGGAG	1 cut(s)	2361
HaeII	RGCGCY	2 cut(s)	134, 2675
HaeIII	GGCC	7 cut(s)	24, 171, 523, 1041, 1122, 2684, 3342
HapII	CCGG	2 cut(s)	21, 116
HgaI	GACGC	1 cut(s)	2999
HhaI	GCGC	4 cut(s)	133, 184, 2674, 3102
Hin1I	GRCGYC	2 cut(s)	863, 3010
Hin6I	GCGC	4 cut(s)	131, 182, 2672, 3100
HinP1I	GCGC	4 cut(s)	131, 182, 2672, 3100
HincII	GTYRAC	4 cut(s)	515, 1294, 1699, 2839
HindII	GTYRAC	4 cut(s)	515, 1294, 1699, 2839
HindIII	AAGCTT	1 cut(s)	1928
HinfI	GANTC	8 cut(s)	54, 740, 766, 1453, 1717, 2574, 2726, 3179
HpaI	GTTAAC	1 cut(s)	2839
HpaII	CCGG	2 cut(s)	21, 116
HphI	GGTGA	3 cut(s)	1039, 1814, 1976
Hpy166II	GTNNAC	8 cut(s)	92, 100, 515, 1294, 1699, 2839, 3113, 3323
Hpy8I	GTNNAC	8 cut(s)	92, 100, 515, 1294, 1699, 2839, 3113, 3323
Hpy99I	CGWCG	1 cut(s)	766
HpyAV	CCTTC	6 cut(s)	719, 1408, 1801, 2888, 3056, 3073
HpyCH4III	ACNGT	9 cut(s)	77, 301, 652, 1765, 1859, 2325, 2497, 2517, 2596
HpyCH4IV	ACGT	6 cut(s)	96, 426, 863, 2206, 2786, 3353
HpyF10VI	GCNNNNNNNGC	6 cut(s)	293, 335, 799, 1112, 1226, 1676
HpySE526I	ACGT	6 cut(s)	96, 426, 863, 2206, 2786, 3353
Hsp92I	GRCGYC	2 cut(s)	863, 3010
HspAI	GCGC	4 cut(s)	131, 182, 2672, 3100
KroI	GCCGGC	1 cut(s)	20
KroNI	GCCGGC	1 cut(s)	22
Ksp22I	TGATCA	4 cut(s)	532, 1776, 2089, 3154
KspAI	GTTAAC	1 cut(s)	2839
LmnI	GCTCC	7 cut(s)	268, 335, 406, 1226, 1274, 1867, 2910
Lsp1109I	GCAGC	4 cut(s)	2256, 2494, 2755, 2890
MaeI	CTAG	9 cut(s)	39, 414, 788, 2282, 2319, 2712, 2750, 2765, 3000
MaeII	ACGT	6 cut(s)	96, 426, 863, 2206, 2786, 3353
MaeIII	GTNAC	5 cut(s)	422, 634, 741, 2098, 2524
MbiI	CCGCTC	3 cut(s)	200, 271, 2224
MfeI	CAATTG	1 cut(s)	2012
MflI	RGATCY	4 cut(s)	1357, 1820, 2995, 3191
MhlI	GDGCHC	4 cut(s)	46, 113, 1605, 2907
MlyI	GAGTC	2 cut(s)	749, 760
MmeI	TCCRAC	1 cut(s)	652
Mph1103I	ATGCAT	3 cut(s)	2130, 2386, 2586
MroNI	GCCGGC	1 cut(s)	20
MroXI	GAANNNNTTC	2 cut(s)	2660, 3270
MseI	TTAA	9 cut(s)	1128, 1139, 1932, 2610, 2838, 2949, 2982, 3278, 3308
MslI	CAYNNNNRTG	3 cut(s)	2123, 3204, 3352
MspA1I	CMGCKG	2 cut(s)	2247, 2881
MspI	CCGG	2 cut(s)	21, 116
MspR9I	CCNGG	2 cut(s)	2552, 2843
MunI	CAATTG	1 cut(s)	2012
Mva1269I	GAATGC	1 cut(s)	1411
MvaI	CCWGG	2 cut(s)	2552, 2843
MvnI	CGCG	2 cut(s)	105, 182
MwoI	GCNNNNNNNGC	6 cut(s)	293, 335, 799, 1112, 1226, 1676
NaeI	GCCGGC	1 cut(s)	22
NgoMIV	GCCGGC	1 cut(s)	20
NlaIV	GGNNCC	5 cut(s)	9, 172, 1173, 2556, 3088
NmeAIII	GCCGAG	3 cut(s)	861, 1299, 2626
NmuCI	GTSAC	3 cut(s)	634, 741, 2098
NsiI	ATGCAT	3 cut(s)	2130, 2386, 2586
NspI	RCATGY	5 cut(s)	333, 1220, 2122, 2132, 2150
NspV	TTCGAA	2 cut(s)	126, 446
OliI	CACNNNNGTG	1 cut(s)	3352
PaeI	GCATGC	3 cut(s)	333, 1220, 2150
PagI	TCATGA	4 cut(s)	769, 1906, 2499, 2577
PciI	ACATGT	1 cut(s)	2118
PcsI	WCGNNNNNNNCGW	2 cut(s)	102, 3121
PctI	GAATGC	1 cut(s)	1411
PdiI	GCCGGC	1 cut(s)	22
PdmI	GAANNNNTTC	2 cut(s)	2660, 3270
PfeI	GAWTC	6 cut(s)	54, 1453, 1717, 2574, 2726, 3179
PkrI	GCNGC	8 cut(s)	26, 178, 181, 186, 2246, 2509, 2770, 2880
PleI	GAGTC	2 cut(s)	748, 760
PmaCI	CACGTG	1 cut(s)	97
PmlI	CACGTG	1 cut(s)	97
PpsI	GAGTC	2 cut(s)	748, 760
Ppu21I	YACGTR	1 cut(s)	97
PpuMI	RGGWCCY	3 cut(s)	728, 1172, 3087
PscI	ACATGT	1 cut(s)	2118
PshAI	GACNNNNGTC	1 cut(s)	1170
PshBI	ATTAAT	1 cut(s)	2949
PsiI	TTATAA	1 cut(s)	2541
Psp124BI	GAGCTC	1 cut(s)	113
Psp1406I	AACGTT	1 cut(s)	426
Psp5II	RGGWCCY	3 cut(s)	728, 1172, 3087
Psp6I	CCWGG	2 cut(s)	2550, 2841
PspCI	CACGTG	1 cut(s)	97
PspFI	CCCAGC	1 cut(s)	2289
PspGI	CCWGG	2 cut(s)	2550, 2841
PspN4I	GGNNCC	5 cut(s)	9, 172, 1173, 2556, 3088
PspPI	GGNCC	9 cut(s)	100, 170, 521, 728, 1039, 1172, 1312, 2555, 3087
PspPPI	RGGWCCY	3 cut(s)	728, 1172, 3087
PstI	CTGCAG	1 cut(s)	1357
PsuI	RGATCY	4 cut(s)	1357, 1820, 2995, 3191
PvuII	CAGCTG	2 cut(s)	2247, 2881
RsaI	GTAC	6 cut(s)	275, 1497, 2234, 2514, 2549, 2590
RsaNI	GTAC	6 cut(s)	274, 1496, 2233, 2513, 2548, 2589
RseI	CAYNNNNRTG	3 cut(s)	2123, 3204, 3352
SacI	GAGCTC	1 cut(s)	113
SaqAI	TTAA	9 cut(s)	1128, 1139, 1932, 2610, 2838, 2949, 2982, 3278, 3308
SatI	GCNGC	8 cut(s)	25, 177, 180, 185, 2245, 2508, 2769, 2879
Sau96I	GGNCC	9 cut(s)	100, 170, 521, 728, 1039, 1172, 1312, 2555, 3087
SchI	GAGTC	2 cut(s)	749, 760
ScrFI	CCNGG	2 cut(s)	2552, 2843
SduI	GDGCHC	4 cut(s)	46, 113, 1605, 2907
SfcI	CTRYAG	4 cut(s)	1221, 1353, 1527, 2595
SfuI	TTCGAA	2 cut(s)	126, 446
SinI	GGWCC	6 cut(s)	100, 728, 1172, 1312, 2555, 3087
SmiMI	CAYNNNNRTG	3 cut(s)	2123, 3204, 3352
SmlI	CTYRAG	2 cut(s)	2942, 3210
SmoI	CTYRAG	2 cut(s)	2942, 3210
SphI	GCATGC	3 cut(s)	333, 1220, 2150
SspI	AATATT	1 cut(s)	856
SspMI	CTAG	9 cut(s)	39, 414, 788, 2282, 2319, 2712, 2750, 2765, 3000
SstI	GAGCTC	1 cut(s)	113
StyD4I	CCNGG	2 cut(s)	2550, 2841
TaaI	ACNGT	9 cut(s)	77, 301, 652, 1765, 1859, 2325, 2497, 2517, 2596
TaiI	ACGT	6 cut(s)	99, 429, 866, 2209, 2789, 3356
TauI	GCSGC	4 cut(s)	27, 179, 182, 187
TfiI	GAWTC	6 cut(s)	54, 1453, 1717, 2574, 2726, 3179
Tru1I	TTAA	9 cut(s)	1128, 1139, 1932, 2610, 2838, 2949, 2982, 3278, 3308
Tru9I	TTAA	9 cut(s)	1128, 1139, 1932, 2610, 2838, 2949, 2982, 3278, 3308
TscAI	CASTG	6 cut(s)	94, 172, 750, 1698, 1821, 2314
TseFI	GTSAC	3 cut(s)	634, 741, 2098
TseI	GCWGC	4 cut(s)	2244, 2507, 2768, 2878
Tsp45I	GTSAC	3 cut(s)	634, 741, 2098
TspGWI	ACGGA	5 cut(s)	1646, 1868, 2459, 2987, 3130
TspRI	CASTG	6 cut(s)	94, 172, 750, 1698, 1821, 2314
VpaK11BI	GGWCC	6 cut(s)	100, 728, 1172, 1312, 2555, 3087
VspI	ATTAAT	1 cut(s)	2949
XagI	CCTNNNNNAGG	1 cut(s)	3094
XapI	RAATTY	3 cut(s)	1753, 1987, 3047
XbaI	TCTAGA	4 cut(s)	787, 2318, 2711, 2749
XceI	RCATGY	5 cut(s)	333, 1220, 2122, 2132, 2150
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XmnI	GAANNNNTTC	2 cut(s)	2660, 3270
XspI	CTAG	9 cut(s)	39, 414, 788, 2282, 2319, 2712, 2750, 2765, 3000
ZraI	GACGTC	1 cut(s)	864
Zsp2I	ATGCAT	3 cut(s)	2130, 2386, 2586

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.