Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005477

Physical Location & Seq

Genomic Coordinates Reverse (-)

41421409 .. 41429068

Gene Structure 7660 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1296700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3372 bp

ATGGAGGAGCCACCGTCAGCCACCGCGGCGGAACCGTCGACGACTGTCCTCCGTAAGAGGACTAGGGGTCGGACTCAGACCACTGAGAACCCCGAGAGGACCACCGCCGGGAGCGACCAGGAGGAGCGCGAGAGCTCACCCGATGACTTCAAAGAAGCTCGTCCTAGAGCTAAACGAGGGCGACCTGCCGCTGCCGCTGCCGCTGTCCCTTCCCCCGCCGCACCGCAGAAACTCGCCGGCCTCACCTTGATTGAGGTCATCAAGGGCAATGGGAAACTTATCCCTCAAGCAGTCAAGCTTTGGGTGGAGCGATATGAAAAGGATCCAAAACCTGCTATGGTTGAACTTTTGATGATGCTGTTTGAGGCATGTGGGGCCAAGTATCAATTCAAAGGAGAGTTGCTGGACGAGATCGATGTTGATGATGTTGTGGTTGCTCTTGTCGAGCTTGCTAGAAGTGGTAATGTTGAAGATTACCAAAGTTCGAAAAAGAAGGAATTTAAGAACTTCAAAGAAAATCTTCAATCTTTCTGGGACAATTTGGTCCGTGAGTGTCAACATGGGCCATTGGTTGATCAGATCTTGTTCGAGAAATGCATGGATTATATAATTGCTTTGTCATGCACTCCTCCAAGAGTTTATCGTCAAGCTGCTACTTTGACGGGTCTCCAACTTGTCACATCGTTTATATCTGTTGCTAACACCCTTGGTACTCAGCGAGAAACTACTCGTAGACAATTAGATGCTGAAAAAAAGAAACAAACTGAGGGGCCTCGTGTGGAATCACTGAACAAGAGGTTTTCAACGACTCATGATAAGATAACTATACTAGAACAGATGATGCGCAAGATATTTCAAGGGTTATTTGTGCATCGCTATCGAGACATTGATCCAAATATTCGGATGTCTTGCATAGAGTCATTAGGCGTGTGGATTCTTTCCTATCCATCACTGTTCTTGCAGGATTTATACCTGAAGTATCTTGGATGGACACTAAATGACAAAAGTGCTGGTGTAAGAAAAGCTTCCGTCCTTGCATTGCAAAATCTTTATGAGGTTGATGATAACGTGCCAACTCTTGGTCTATTCACTGAAAGATTTTCTAGTCGGATGATTGATCTTGCTGATGACATCGACATTTCTGTGGCTGTATGTGCAATTGGCCTTGTTAAACAGCTACTAAGACACCAGCTTCTACAAGATGATGATTTGATTCCTTTATATGATCTGCTTATCGATAACCTACCAGAGATCAGGCATGCTATAGGGGCATTAGTGTATGAACACTTGATTTCTCAGAAGTTCAATAGCTCCCAATCTGGTGCAAAAGGAGATGGCAGCAATTCTTCTGAGGTCCATCTTGGCAGAATGTTGAAAATTCTAAATGAGTTCTCCGCCGATCCAATTCTAAGCATCTATGTCATTGATGATGTTTGGGAGTACATGAAGGCAATGAAGGATTGGAAGTGTATTATCTCCATGCTTTTGGATGAGAATCCATTGATCGAGCTTACTGATGAGGATGCAACAAACTTGGTACGACTTCTTTGTTCATCTGTCAAAAAGGCTGTAGGAGAGAGGATTGTTCCTGCCACCGATAACCGAAAGCCACACTACAGTAAAGCTCAAAAAGAAGTATTTGAACACAACAGACGAGATATAACTCTTGCCATGATGAAGAGTTACCCATTACTTCTACGGAAGTTCATGGCTGACAAAACAAAAGTACCATCACTGATTGAAATTATTGTGCATATGAATCTTGAGCTATATTCCCTGAAGAGGCAGGAGCAGAACTTCAAAACTGTTCTTCAACTCATCAAGGAGGCATTTTTTAAACATGGTGAGAGGGAGGCACTGAGATCCTGCGTAAATGCTATCAACCTTTGTTCCACGGAGAGTCAAGGGGAACTAAAAGATTTTGCGCGTAATACACTAAAGGAACTTCAGGATGAGCTTATTGCCAAGCTTAAATCTGCAATGAAAGAAGTGGCGGATGGTGGTGATGAATATCCTCTTCTGGTGAATTTGAAAAGGTTGTATGAGCTTCAATTGACAAGAGCTGTTTCTACTGATACCTTGTATGAAGATCTTGTCTCAACTCTTCAGAGTCATACAAGCAGGGACCATGAGGTTATCAGTTTTCTGCTTCTCAACACGTCTTTGCATTTGGAATGGTCTCTGCAGTCAATCATCACTAGTGAGACTGTCTCTGAAGCATCTTTGTCTTCTCTATTAGCGAAACGCAATTCCTTGTCTCAGCAACTAGAGTACTTTCTTAACGGCCCTCCTGAATTGGAGGGTAATCCTGCTAATCAGCTAGCTTGCAGGGTTTGTATTCTGCTATCGGAGTTGTGGTTTATGTTTAGAAAGACACTTTTTGCTTCAACAAATCTGGAAAGGTTGGGATATCATCCAGATGCATCCATTCTTAAAAAATTTTGGAACCTATGTGTGCAACAGCTCAGTATTTCAGATGAGACAGAAGATGAATATGCGAACCGAGAGTATATTGAGGAGGCAAACAGAGAAGCAGTAATTATTGCTGCTGCAAAGTTGGTTGCTAACGATGTAGGTTCTAAGGAATATCTTGGTCCGGAGATTATCTCTCGATTTTTGATACATGGATCATCTGTAGCAGAGATTATTAAGAATCTCATTACCTGTCTAAAGAAGAAAGATGACGATCTTCCCAAAATTTTCTTTGAAGCACTGAAAAAGGCCTACCAGCGGTATGTGGTTGAACTTTCTGAGAGTGATGATGATGAATCTTCAGCAAGCAAGCGTTTTCAAGAATGTAAAGAATTGGCTGCTCGGATTTCTGGGATGTTTATAGGTGCTGCCAGGAACAAGCACAGATCAAACATTCTAAAAATTGTTAGTGATGGCATTGAATATGCATTTCTAGATGCACCGAAGCAGTTATCTTTTCTGGAAGGCGCTGTGATCCATTTCGTATCCAAATTGCCAACATCTGATATCCTGGACATTGTTAAGGATGTCCAGAGCAGAACAGAAAATATAAATACGGATGAAGATCCCAGTGGCTGGCGCCCCTATCACACGTTTGTTGACAACTTGCTTGAGAAATATGCAAAGAATGAAGGTGTCCAAGATGAACAGGATGCCCCCTCTGTAAAGCGCAGGGGTCGCCCACGCAAAAAGCGTCGGGGAAAGGGACTCTTTGATGAGCAGAGTTCGAGTGAAGAAGAGGATTTAGTAAGCGCATCGGACCATGAAAATGCTCAAGATGAAGAAAACAAGCAAGACGACGAGGATGACGATGAAGACGCTCCCTTGATACAGTCATTTAGGTCATCCGCCAAGTTGAGGGCATTGAGAGTTGCAAGAGAGGAAACAAAGGCCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007127 GO:0007135 GO:0008150 GO:0008608 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0045132 GO:0045143 GO:0045144 GO:0048285 GO:0051177 GO:0051276 GO:0051316 GO:0051321 GO:0051455 GO:0051754 GO:0061982 GO:0061983 GO:0070192 GO:0070601 GO:0071840 GO:0098813 GO:0140013 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04110 ko04111 ko04113 map04110 map04111 map04113

Pfam Domains

Protein Families

STAG

Protein Analysis

1124

Amino Acids

127.42

Weight (kDa)

5.49

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
STAG	PF08514	158 - 265	1.2e-28	STAG domain
SCD	PF21581	294 - 367	2.2e-29	Stromalin conservative domain
HEAT_SCC3-SA	PF24571	473 - 726	2.6e-73	Cohesin subunit SCC3/SA, HEAT-repeats domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47980
fragaria_vesca	FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570 FvH4_2g24570
malus_domestica	MD11G1296700.v1.1
prunus_persica	Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1 Prupe.8G250900_v2.0.a1
pyrus_communis	pycom11g26100
rosa_chinensis	RchiOBHm_Chr6g0292391
rosa_laevigata	RLG00000012032
rosa_multiflora	Rmu_sc0012226.1_g000001 Rmu_sc0023540.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026580 Rroxscaffold_7G00175100
rosa_rugosa	Rorug06G0226100 Rorug06G0226200 Rorug06G0226300
rosa_samantha	Rh6AG339600 Rh6BG346800 Rh6CG353600 Rh6DG339600
rosa_wichuraiana	Rw6G029550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	542
Acc16I	TGCGCA	1 cut(s)	845
Acc36I	ACCTGC	2 cut(s)	193, 340
AccB1I	GGYRCC	1 cut(s)	3051
AccB7I	CCANNNNNTGG	2 cut(s)	1079, 3048
AccI	GTMKAC	2 cut(s)	38, 733
AccII	CGCG	3 cut(s)	26, 129, 1927
AccIII	TCCGGA	1 cut(s)	2596
AclWI	GGATC	8 cut(s)	317, 330, 884, 1394, 1857, 2635, 2941, 3032
AcsI	RAATTY	5 cut(s)	497, 1377, 2026, 2438, 2697
AcuI	CTGAAG	6 cut(s)	995, 1799, 1931, 2090, 2235, 2757
AcyI	GRCGYC	1 cut(s)	3052
AfaI	GTAC	5 cut(s)	712, 1442, 1539, 1728, 2273
AfiI	CCNNNNNNNGG	6 cut(s)	108, 1079, 1782, 2730, 3048, 3330
AflIII	ACRYGT	2 cut(s)	2157, 3063
AhdI	GACNNNNNGTC	1 cut(s)	66
AhlI	ACTAGT	1 cut(s)	2198
AjiI	CACGTC	1 cut(s)	2160
AjnI	CCWGG	3 cut(s)	117, 2843, 2982
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
Alw21I	GWGCWC	1 cut(s)	137
Alw26I	GTCTC	8 cut(s)	671, 876, 2101, 2184, 2198, 2215, 2262, 2474
AlwI	GGATC	8 cut(s)	317, 330, 884, 1394, 1857, 2635, 2941, 3032
AlwNI	CAGNNNCTG	1 cut(s)	3048
Ama87I	CYCGRG	1 cut(s)	92
Aor13HI	TCCGGA	1 cut(s)	2596
AoxI	GGCC	8 cut(s)	238, 375, 563, 770, 1162, 2284, 2721, 3363
ApeKI	GCWGC	8 cut(s)	191, 197, 650, 1338, 2546, 2549, 2810, 2840
ApoI	RAATTY	5 cut(s)	497, 1377, 2026, 2438, 2697
Asp700I	GAANNNNTTC	2 cut(s)	519, 1024
AspLEI	GCGC	7 cut(s)	129, 846, 1927, 2942, 3054, 3144, 3227
AsuC2I	CCSGG	1 cut(s)	109
AsuHPI	GGTGA	5 cut(s)	129, 235, 1856, 2015, 2035
AsuII	TTCGAA	1 cut(s)	485
AsuNHI	GCTAGC	1 cut(s)	2320
AvaI	CYCGRG	1 cut(s)	92
AvaII	GGWCC	6 cut(s)	99, 544, 1354, 2125, 2594, 3232
BaeI	ACNNNNGTAYC	2 cut(s)	1710, 1743
BamHI	GGATCC	1 cut(s)	322
BanI	GGYRCC	1 cut(s)	3051
BanII	GRGCYC	1 cut(s)	137
BauI	CACGAG	1 cut(s)	774
BbsI	GAAGAC	2 cut(s)	2220, 3294
Bbv12I	GWGCWC	1 cut(s)	137
BbvI	GCAGC	8 cut(s)	178, 184, 637, 1350, 2533, 2536, 2797, 2827
BccI	CCATC	7 cut(s)	955, 981, 1328, 1365, 1738, 1991, 2879
BceAI	ACGGC	1 cut(s)	2299
BcgI	CGANNNNNNTGC	2 cut(s)	860, 894
BciT130I	CCWGG	3 cut(s)	119, 2845, 2984
BciVI	GTATCC	1 cut(s)	2968
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	1 cut(s)	109
BcoDI	GTCTC	8 cut(s)	671, 876, 2101, 2184, 2198, 2215, 2262, 2474
BcuI	ACTAGT	1 cut(s)	2198
BfaI	CTAG	9 cut(s)	63, 165, 453, 830, 1104, 2199, 2267, 2321, 2906
BfmI	CTRYAG	5 cut(s)	1263, 1569, 1615, 2183, 2634
BfoI	RGCGCY	2 cut(s)	2943, 3055
BfuAI	ACCTGC	2 cut(s)	193, 340
BfuI	GTATCC	1 cut(s)	2968
BglI	GCCNNNNNGGC	1 cut(s)	26
BglII	AGATCT	2 cut(s)	579, 2089
BmcAI	AGTACT	1 cut(s)	2273
Bme1390I	CCNGG	4 cut(s)	109, 119, 2845, 2984
Bme18I	GGWCC	6 cut(s)	99, 544, 1354, 2125, 2594, 3232
BmeRI	GACNNNNNGTC	1 cut(s)	66
BmeT110I	CYCGRG	1 cut(s)	92
BmgBI	CACGTC	1 cut(s)	2160
BmiI	GGNNCC	8 cut(s)	9, 33, 324, 376, 771, 2126, 2447, 3053
BmrFI	CCNGG	4 cut(s)	109, 119, 2845, 2984
BmrI	ACTGGG	1 cut(s)	3036
BmtI	GCTAGC	1 cut(s)	2324
BmuI	ACTGGG	1 cut(s)	3036
BoxI	GACNNNNGTC	1 cut(s)	44
BpiI	GAAGAC	2 cut(s)	2220, 3294
BplI	GAGNNNNNCTC	4 cut(s)	2195, 2227, 2591, 2623
Bpu14I	TTCGAA	1 cut(s)	485
BpuEI	CTTGAG	4 cut(s)	270, 1784, 3104, 3231
BpuMI	CCSGG	1 cut(s)	109
Bsa29I	ATCGAT	2 cut(s)	414, 1236
BsaBI	GATNNNNATC	4 cut(s)	1494, 2010, 2685, 3036
BsaHI	GRCGYC	1 cut(s)	3052
BsaI	GGTCTC	2 cut(s)	671, 2184
BsaJI	CCNNGG	3 cut(s)	24, 706, 1893
BsaWI	WCCGGW	1 cut(s)	2596
BsaXI	ACNNNNNCTCC	3 cut(s)	29, 2342, 2372
Bsc4I	CCNNNNNNNGG	6 cut(s)	108, 1079, 1782, 2730, 3048, 3330
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	1 cut(s)	3042
Bse3DI	GCAATG	4 cut(s)	274, 1037, 1458, 1986
Bse8I	GATNNNNATC	4 cut(s)	1494, 2010, 2685, 3036
BseAI	TCCGGA	1 cut(s)	2596
BseBI	CCWGG	3 cut(s)	119, 2845, 2984
BseCI	ATCGAT	2 cut(s)	414, 1236
BseDI	CCNNGG	3 cut(s)	24, 706, 1893
BseJI	GATNNNNATC	4 cut(s)	1494, 2010, 2685, 3036
BseLI	CCNNNNNNNGG	6 cut(s)	108, 1079, 1782, 2730, 3048, 3330
BseMI	GCAATG	4 cut(s)	274, 1037, 1458, 1986
BseNI	ACTGG	1 cut(s)	3042
BseRI	GAGGAG	4 cut(s)	20, 137, 618, 2531
BseXI	GCAGC	8 cut(s)	178, 184, 637, 1350, 2533, 2536, 2797, 2827
Bsh1236I	CGCG	3 cut(s)	26, 129, 1927
BshFI	GGCC	8 cut(s)	240, 377, 565, 772, 1164, 2286, 2723, 3365
BshNI	GGYRCC	1 cut(s)	3051
BshVI	ATCGAT	2 cut(s)	414, 1236
BsiHKAI	GWGCWC	1 cut(s)	137
BsiHKCI	CYCGRG	1 cut(s)	92
BsiSI	CCGG	3 cut(s)	108, 237, 2597
BslFI	GGGAC	4 cut(s)	191, 548, 2138, 3192
BslI	CCNNNNNNNGG	6 cut(s)	108, 1079, 1782, 2730, 3048, 3330
BsmAI	GTCTC	8 cut(s)	671, 876, 2101, 2184, 2198, 2215, 2262, 2474
BsmFI	GGGAC	4 cut(s)	191, 548, 2138, 3192
BsnI	GGCC	8 cut(s)	240, 377, 565, 772, 1164, 2286, 2723, 3365
Bso31I	GGTCTC	2 cut(s)	671, 2184
BsoBI	CYCGRG	1 cut(s)	92
Bsp119I	TTCGAA	1 cut(s)	485
Bsp1286I	GDGCHC	1 cut(s)	137
Bsp13I	TCCGGA	1 cut(s)	2596
BspANI	GGCC	8 cut(s)	240, 377, 565, 772, 1164, 2286, 2723, 3365
BspDI	ATCGAT	2 cut(s)	414, 1236
BspEI	TCCGGA	1 cut(s)	2596
BspFNI	CGCG	3 cut(s)	26, 129, 1927
BspHI	TCATGA	1 cut(s)	811
BspLI	GGNNCC	8 cut(s)	9, 33, 324, 376, 771, 2126, 2447, 3053
BspMAI	CTGCAG	1 cut(s)	2187
BspMI	ACCTGC	2 cut(s)	193, 340
BspOI	GCTAGC	1 cut(s)	2324
BspPI	GGATC	8 cut(s)	317, 330, 884, 1394, 1857, 2635, 2941, 3032
BspT104I	TTCGAA	1 cut(s)	485
BspT107I	GGYRCC	1 cut(s)	3051
BspTNI	GGTCTC	2 cut(s)	671, 2184
BsrDI	GCAATG	4 cut(s)	274, 1037, 1458, 1986
BsrFI	RCCGGY	1 cut(s)	236
BsrI	ACTGG	1 cut(s)	3042
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	3 cut(s)	24, 706, 1893
BssNI	GRCGYC	1 cut(s)	3052
BssSI	CACGAG	1 cut(s)	774
BssT1I	CCWWGG	1 cut(s)	706
Bst2BI	CACGAG	1 cut(s)	774
Bst2UI	CCWGG	3 cut(s)	119, 2845, 2984
Bst4CI	ACNGT	8 cut(s)	15, 36, 46, 954, 1619, 1807, 2209, 3306
Bst6I	CTCTTC	5 cut(s)	1673, 1775, 2022, 2109, 3204
BstACI	GRCGYC	1 cut(s)	3052
BstBI	TTCGAA	1 cut(s)	485
BstC8I	GCNNGC	8 cut(s)	238, 450, 1260, 2322, 2326, 2779, 2783, 3050
BstDSI	CCRYGG	2 cut(s)	24, 1893
BstFNI	CGCG	3 cut(s)	26, 129, 1927
BstH2I	RGCGCY	2 cut(s)	2943, 3055
BstHHI	GCGC	7 cut(s)	129, 846, 1927, 2942, 3054, 3144, 3227
BstMAI	GTCTC	8 cut(s)	671, 876, 2101, 2184, 2198, 2215, 2262, 2474
BstMWI	GCNNNNNNNGC	8 cut(s)	26, 194, 197, 200, 374, 1154, 1268, 3150
BstNI	CCWGG	3 cut(s)	119, 2845, 2984
BstNSI	RCATGY	2 cut(s)	372, 1262
BstPAI	GACNNNNGTC	1 cut(s)	44
BstSCI	CCNGG	4 cut(s)	107, 117, 2843, 2982
BstSFI	CTRYAG	5 cut(s)	1263, 1569, 1615, 2183, 2634
BstUI	CGCG	3 cut(s)	26, 129, 1927
BstV1I	GCAGC	8 cut(s)	178, 184, 637, 1350, 2533, 2536, 2797, 2827
BstV2I	GAAGAC	2 cut(s)	2220, 3294
BstX2I	RGATCY	5 cut(s)	322, 579, 1862, 2089, 3037
BstXI	CCANNNNNNTGG	1 cut(s)	1486
BstYI	RGATCY	5 cut(s)	322, 579, 1862, 2089, 3037
Bsu15I	ATCGAT	2 cut(s)	414, 1236
BsuI	GTATCC	1 cut(s)	2968
BsuRI	GGCC	8 cut(s)	240, 377, 565, 772, 1164, 2286, 2723, 3365
BsuTUI	ATCGAT	2 cut(s)	414, 1236
BtgI	CCRYGG	2 cut(s)	24, 1893
BtgZI	GCGATG	1 cut(s)	857
BtrI	CACGTC	1 cut(s)	2160
BtsIMutI	CAGTG	8 cut(s)	81, 785, 950, 1089, 1733, 1856, 2711, 3049
BveI	ACCTGC	2 cut(s)	193, 340
Cac8I	GCNNGC	8 cut(s)	238, 450, 1260, 2322, 2326, 2779, 2783, 3050
CaiI	CAGNNNCTG	1 cut(s)	3048
CciI	TCATGA	1 cut(s)	811
CfoI	GCGC	7 cut(s)	129, 846, 1927, 2942, 3054, 3144, 3227
Cfr10I	RCCGGY	1 cut(s)	236
Cfr42I	CCGCGG	1 cut(s)	27
ClaI	ATCGAT	2 cut(s)	414, 1236
CseI	GACGC	2 cut(s)	3155, 3299
Csp6I	GTAC	5 cut(s)	711, 1441, 1538, 1727, 2272
CviQI	GTAC	5 cut(s)	711, 1441, 1538, 1727, 2272
DinI	GGCGCC	1 cut(s)	3053
DraI	TTTAAA	1 cut(s)	1837
DrdI	GACNNNNNNGTC	1 cut(s)	542
DriI	GACNNNNNGTC	1 cut(s)	66
DseDI	GACNNNNNNGTC	1 cut(s)	542
Eam1104I	CTCTTC	5 cut(s)	1673, 1775, 2022, 2109, 3204
Eam1105I	GACNNNNNGTC	1 cut(s)	66
EarI	CTCTTC	5 cut(s)	1673, 1775, 2022, 2109, 3204
EciI	GGCGGA	4 cut(s)	44, 1384, 2009, 3310
Ecl136II	GAGCTC	1 cut(s)	135
Eco130I	CCWWGG	1 cut(s)	706
Eco147I	AGGCCT	1 cut(s)	2723
Eco24I	GRGCYC	1 cut(s)	137
Eco31I	GGTCTC	2 cut(s)	671, 2184
Eco32I	GATATC	2 cut(s)	2411, 2980
Eco47I	GGWCC	6 cut(s)	99, 544, 1354, 2125, 2594, 3232
Eco53kI	GAGCTC	1 cut(s)	135
Eco57I	CTGAAG	6 cut(s)	995, 1799, 1931, 2090, 2235, 2757
Eco88I	CYCGRG	1 cut(s)	92
EcoICRI	GAGCTC	1 cut(s)	135
EcoO109I	RGGNCCY	1 cut(s)	770
EcoRII	CCWGG	3 cut(s)	117, 2843, 2982
EcoRV	GATATC	2 cut(s)	2411, 2980
EcoT14I	CCWWGG	1 cut(s)	706
EcoT22I	ATGCAT	3 cut(s)	599, 2425, 2902
EcoT38I	GRGCYC	1 cut(s)	137
EgeI	GGCGCC	1 cut(s)	3053
EheI	GGCGCC	1 cut(s)	3053
ErhI	CCWWGG	1 cut(s)	706
FalI	AAGNNNNNCTT	6 cut(s)	504, 536, 1009, 1041, 3167, 3199
FaqI	GGGAC	4 cut(s)	191, 548, 2138, 3192
FauI	CCCGC	1 cut(s)	223
FauNDI	CATATG	1 cut(s)	1755
FbaI	TGATCA	1 cut(s)	574
FblI	GTMKAC	2 cut(s)	38, 733
FriOI	GRGCYC	1 cut(s)	137
FspBI	CTAG	9 cut(s)	63, 165, 453, 830, 1104, 2199, 2267, 2321, 2906
FspI	TGCGCA	1 cut(s)	845
GlaI	GCGC	7 cut(s)	128, 845, 1926, 2941, 3053, 3143, 3226
HaeII	RGCGCY	2 cut(s)	2943, 3055
HaeIII	GGCC	8 cut(s)	240, 377, 565, 772, 1164, 2286, 2723, 3365
HapII	CCGG	3 cut(s)	108, 237, 2597
HgaI	GACGC	2 cut(s)	3155, 3299
HhaI	GCGC	7 cut(s)	129, 846, 1927, 2942, 3054, 3144, 3227
Hin1I	GRCGYC	1 cut(s)	3052
Hin6I	GCGC	7 cut(s)	127, 844, 1925, 2940, 3052, 3142, 3225
HinP1I	GCGC	7 cut(s)	127, 844, 1925, 2940, 3052, 3142, 3225
HincII	GTYRAC	3 cut(s)	39, 557, 3073
HindII	GTYRAC	3 cut(s)	39, 557, 3073
HindIII	AAGCTT	3 cut(s)	296, 1023, 1967
HpaII	CCGG	3 cut(s)	108, 237, 2597
HphI	GGTGA	5 cut(s)	129, 235, 1856, 2015, 2035
Hpy166II	GTNNAC	4 cut(s)	39, 557, 734, 3073
Hpy8I	GTNNAC	4 cut(s)	39, 557, 734, 3073
Hpy99I	CGWCG	4 cut(s)	40, 43, 3171, 3275
HpyAV	CCTTC	6 cut(s)	219, 487, 1441, 1450, 2930, 3098
HpyCH4III	ACNGT	8 cut(s)	15, 36, 46, 954, 1619, 1807, 2209, 3306
HpyCH4IV	ACGT	3 cut(s)	1068, 2159, 3065
HpyF10VI	GCNNNNNNNGC	8 cut(s)	26, 194, 197, 200, 374, 1154, 1268, 3150
HpySE526I	ACGT	3 cut(s)	1068, 2159, 3065
Hsp92I	GRCGYC	1 cut(s)	3052
HspAI	GCGC	7 cut(s)	127, 844, 1925, 2940, 3052, 3142, 3225
KasI	GGCGCC	1 cut(s)	3051
Kpn2I	TCCGGA	1 cut(s)	2596
KroI	GCCGGC	1 cut(s)	236
KroNI	GCCGGC	1 cut(s)	238
Ksp22I	TGATCA	1 cut(s)	574
KspI	CCGCGG	1 cut(s)	27
LmnI	GCTCC	7 cut(s)	7, 111, 124, 307, 1316, 1789, 3298
Lsp1109I	GCAGC	8 cut(s)	178, 184, 637, 1350, 2533, 2536, 2797, 2827
MaeI	CTAG	9 cut(s)	63, 165, 453, 830, 1104, 2199, 2267, 2321, 2906
MaeII	ACGT	3 cut(s)	1068, 2159, 3065
MaeIII	GTNAC	2 cut(s)	676, 1682
MfeI	CAATTG	2 cut(s)	1158, 2051
MflI	RGATCY	5 cut(s)	322, 579, 1862, 2089, 3037
MhlI	GDGCHC	1 cut(s)	137
Mly113I	GGCGCC	1 cut(s)	3052
MlyI	GAGTC	6 cut(s)	67, 802, 926, 1909, 2119, 3174
MmeI	TCCRAC	3 cut(s)	50, 694, 1088
Mph1103I	ATGCAT	3 cut(s)	599, 2425, 2902
MroI	TCCGGA	1 cut(s)	2596
MroNI	GCCGGC	1 cut(s)	236
MroXI	GAANNNNTTC	2 cut(s)	519, 1024
MseI	TTAA	8 cut(s)	501, 1170, 1836, 1971, 2280, 2433, 2649, 2994
MslI	CAYNNNNRTG	3 cut(s)	1142, 2418, 3240
MspA1I	CMGCKG	5 cut(s)	26, 191, 197, 203, 2731
MspI	CCGG	3 cut(s)	108, 237, 2597
MspR9I	CCNGG	4 cut(s)	109, 119, 2845, 2984
MunI	CAATTG	2 cut(s)	1158, 2051
MvaI	CCWGG	3 cut(s)	119, 2845, 2984
MvnI	CGCG	3 cut(s)	26, 129, 1927
MwoI	GCNNNNNNNGC	8 cut(s)	26, 194, 197, 200, 374, 1154, 1268, 3150
NaeI	GCCGGC	1 cut(s)	238
NarI	GGCGCC	1 cut(s)	3052
NciI	CCSGG	1 cut(s)	109
NdeI	CATATG	1 cut(s)	1755
NgoMIV	GCCGGC	1 cut(s)	236
NheI	GCTAGC	1 cut(s)	2320
NlaIV	GGNNCC	8 cut(s)	9, 33, 324, 376, 771, 2126, 2447, 3053
NmuCI	GTSAC	1 cut(s)	676
NsbI	TGCGCA	1 cut(s)	845
NsiI	ATGCAT	3 cut(s)	599, 2425, 2902
NspI	RCATGY	2 cut(s)	372, 1262
NspV	TTCGAA	1 cut(s)	485
PaeI	GCATGC	1 cut(s)	1262
PagI	TCATGA	1 cut(s)	811
PceI	AGGCCT	1 cut(s)	2723
PcsI	WCGNNNNNNNCGW	2 cut(s)	3163, 3279
PdiI	GCCGGC	1 cut(s)	238
PdmI	GAANNNNTTC	2 cut(s)	519, 1024
PfeI	GAWTC	7 cut(s)	782, 934, 1213, 1495, 1759, 2653, 2768
PflMI	CCANNNNNTGG	2 cut(s)	1079, 3048
PfoI	TCCNGGA	1 cut(s)	2982
PleI	GAGTC	6 cut(s)	67, 802, 925, 1908, 2118, 3174
PluTI	GGCGCC	1 cut(s)	3055
PpsI	GAGTC	6 cut(s)	67, 802, 925, 1908, 2118, 3174
PshAI	GACNNNNGTC	1 cut(s)	44
Psp124BI	GAGCTC	1 cut(s)	137
Psp6I	CCWGG	3 cut(s)	117, 2843, 2982
PspGI	CCWGG	3 cut(s)	117, 2843, 2982
PspN4I	GGNNCC	8 cut(s)	9, 33, 324, 376, 771, 2126, 2447, 3053
PstI	CTGCAG	1 cut(s)	2187
PstNI	CAGNNNCTG	1 cut(s)	3048
PsuI	RGATCY	5 cut(s)	322, 579, 1862, 2089, 3037
RsaI	GTAC	5 cut(s)	712, 1442, 1539, 1728, 2273
RsaNI	GTAC	5 cut(s)	711, 1441, 1538, 1727, 2272
RseI	CAYNNNNRTG	3 cut(s)	1142, 2418, 3240
SacI	GAGCTC	1 cut(s)	137
SacII	CCGCGG	1 cut(s)	27
SalI	GTCGAC	1 cut(s)	37
SaqAI	TTAA	8 cut(s)	501, 1170, 1836, 1971, 2280, 2433, 2649, 2994
ScaI	AGTACT	1 cut(s)	2273
SchI	GAGTC	6 cut(s)	67, 802, 926, 1909, 2119, 3174
ScrFI	CCNGG	4 cut(s)	109, 119, 2845, 2984
SduI	GDGCHC	1 cut(s)	137
SfcI	CTRYAG	5 cut(s)	1263, 1569, 1615, 2183, 2634
SfoI	GGCGCC	1 cut(s)	3053
Sfr303I	CCGCGG	1 cut(s)	27
SfuI	TTCGAA	1 cut(s)	485
SgrBI	CCGCGG	1 cut(s)	27
SgrDI	CGTCGACG	1 cut(s)	37
SinI	GGWCC	6 cut(s)	99, 544, 1354, 2125, 2594, 3232
SmiMI	CAYNNNNRTG	3 cut(s)	1142, 2418, 3240
SmlI	CTYRAG	4 cut(s)	285, 1763, 3083, 3246
SmoI	CTYRAG	4 cut(s)	285, 1763, 3083, 3246
SpeI	ACTAGT	1 cut(s)	2198
SphI	GCATGC	1 cut(s)	1262
SseBI	AGGCCT	1 cut(s)	2723
SspDI	GGCGCC	1 cut(s)	3051
SspI	AATATT	1 cut(s)	898
SspMI	CTAG	9 cut(s)	63, 165, 453, 830, 1104, 2199, 2267, 2321, 2906
SstI	GAGCTC	1 cut(s)	137
StuI	AGGCCT	1 cut(s)	2723
StyD4I	CCNGG	4 cut(s)	107, 117, 2843, 2982
StyI	CCWWGG	1 cut(s)	706
TaaI	ACNGT	8 cut(s)	15, 36, 46, 954, 1619, 1807, 2209, 3306
TaiI	ACGT	3 cut(s)	1071, 2162, 3068
TatI	WGTACW	2 cut(s)	1440, 2271
TauI	GCSGC	5 cut(s)	29, 191, 197, 203, 221
TfiI	GAWTC	7 cut(s)	782, 934, 1213, 1495, 1759, 2653, 2768
Tru1I	TTAA	8 cut(s)	501, 1170, 1836, 1971, 2280, 2433, 2649, 2994
Tru9I	TTAA	8 cut(s)	501, 1170, 1836, 1971, 2280, 2433, 2649, 2994
TscAI	CASTG	8 cut(s)	88, 792, 957, 1096, 1740, 1863, 2718, 3049
TseFI	GTSAC	1 cut(s)	676
TseI	GCWGC	8 cut(s)	191, 197, 650, 1338, 2546, 2549, 2810, 2840
Tsp45I	GTSAC	1 cut(s)	676
TspGWI	ACGGA	6 cut(s)	41, 536, 1018, 1714, 1910, 3044
TspRI	CASTG	8 cut(s)	88, 792, 957, 1096, 1740, 1863, 2718, 3049
Van91I	CCANNNNNTGG	2 cut(s)	1079, 3048
VpaK11BI	GGWCC	6 cut(s)	99, 544, 1354, 2125, 2594, 3232
XapI	RAATTY	5 cut(s)	497, 1377, 2026, 2438, 2697
XbaI	TCTAGA	1 cut(s)	2905
XceI	RCATGY	2 cut(s)	372, 1262
XmiI	GTMKAC	2 cut(s)	38, 733
XmnI	GAANNNNTTC	2 cut(s)	519, 1024
XspI	CTAG	9 cut(s)	63, 165, 453, 830, 1104, 2199, 2267, 2321, 2906
ZrmI	AGTACT	1 cut(s)	2273
Zsp2I	ATGCAT	3 cut(s)	599, 2425, 2902

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.