FvH4_2g24670

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011526
Physical Location & Seq
Reverse (-)
20067297 .. 20068949
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24670.t1

Sequence Viewer

Length: 1653 bp
ATGAAGTCGATGAACATCCTGGCATTACTTTTGCTCTCATTGTTATTAGCATCTACCGTTGCAGCTGATGATCAATCCAATATCTTTGATGTGACAAGCTCGAAATATGGTGGAAACCCCGACTCCGATATCACAAAGGCGTTAACAATGGCGTGGACCGATGCATGTGAATCAGGGTCGACGAGTACCGTCCTAGTTCCAACTGGAACATACAAGTTATTGGAACAAATAAGTTTTGAAGGTCCATGCAATGCTCCTATTCAATTTCAGCTCCAAGGAATGTTGCAGGCTCCAGAAGACCGGAGCCAACTTGATGACTCTTGGGTTTACTTTAAGACCATCGACAAGCTCACATTGTCCGGCGGTGGAACTTTCGACGGACAAGGAGAGCTTTCTTGGAAAGAGGACAAGCACTTTCACTCAACTCCCGTCGATGTTAGGTTCAAAACCGTCAACAATTCAGTTATTAGCGACATAACATTTATGGACAGTGCAAAATTTCACATTAGTGTTCATGCCTCCAGCAACATTACGTTTCAACGGGTCACCATCACAGCACCCGAAGACAGTCCTAACACTGACGGAATCCACATCGCCGGTTCGAGCAAGATCACAGTCTCCGACTCTTACATTGCAACCGGTGACGATTGTGTTTCAATTGGTGATGGCGCAACCGAAATCCTAGTGTCCAACATTACTTGCGGACCAGGACATGGGATAAGCATTGGCAGTCTAGGAAAGTATCCAGATGAACAACCAGTGGTCGGTGTCACGGTCAAGAACTGCACACTAACTGGCACAACTAACGGAGCGAGAATCAAGACATGGCCCGATTCTCCGGCAGAAGGCGTTGCTAAGGATATACTTTACCAAGACATCATCATGGTGAATGTGAAGAACCCTATCCTCATCAACCAAAACTACTGCGACAAAGGCAAAATGTGTAAGCAAATGGTTCCATCCAAAGTCCAAATCAGTGACGTGAGATTCAAGAATATAAGGGGCTCATCTTTGTCTCCGGTCGCCGTCAAGCTCGACTGTAGTCAGAGCATGCCGTGTCAGAATGTGGAGCTGTCCGATATTGATCTCGCATATAGTGGAGACGAAAAAGACGAAATCACGTCCGAGTGTAGTAATGTTAAGCCAAGAATTACCGGCGTGACTAAGTCTCTTGCATGTGCAAACCTACGTGACATACCAATAGAGGGTTCACCATCACCGTCGACTTCACCGCCCCTTGAACCGGCACCAGTACAACAGTCACCACCCCCGGCTCCGGTAGCAAAACACCACCATCCACCTCCTCTTTCGCCGCCAGCAGAGGAAACACCTCCTCTTCCTCCAGCAGAGGAAACACCTCCTCTTCCGCCAGTAGAGGAAACACCTCCTCTTCCGCCAGCAGTGGAAACACCTCCTCTTCCGCCAGCAGTGGGAACACCTCCCAAAGTAACATCACATGATCCGTCTCCGCCCCCTCCTTCCCCCTATCCGTATCGTCGTTTAGGACCAACATCCTCCGCTGCCGGCAATAATAATTTGTGGAAAAAAACGATTGTAAGAGTGCCTGTTCTTTTGCTTGTGTATGTGATTGTGCTTGTGATTGTGCTTGTTTCGGCGGTGACGGCTTTGTGTTGTTACTGCACGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

551

Amino Acids

58.69

Weight (kDa)

5.15

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 381 6.1e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1246
AccB7I CCANNNNNTGG 1 cut(s) 713
AccI GTMKAC 2 cut(s) 179, 1223
AclWI GGATC 1 cut(s) 1454
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 2 cut(s) 187, 1254
AfiI CCNNNNNNNGG 7 cut(s) 713, 764, 845, 1205, 1243, 1276, 1430
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 7 cut(s) 239, 263, 445, 539, 657, 991, 1241
AjiI CACGTC 2 cut(s) 982, 1122
AjnI CCWGG 2 cut(s) 18, 706
AjuI GAANNNNNNNTTGG 2 cut(s) 1192, 1224
AleI CACNNNNGTG 1 cut(s) 507
AluBI AGCT 7 cut(s) 65, 99, 271, 349, 391, 1033, 1072
AluI AGCT 7 cut(s) 65, 99, 271, 349, 391, 1033, 1072
Alw26I GTCTC 5 cut(s) 622, 1020, 1095, 1173, 1470
AlwI GGATC 1 cut(s) 1454
AoxI GGCC 1 cut(s) 827
ApeKI GCWGC 2 cut(s) 62, 1520
ApoI RAATTY 1 cut(s) 497
AsiGI ACCGGT 1 cut(s) 638
AspLEI GCGC 1 cut(s) 671
AspS9I GGNCC 5 cut(s) 156, 242, 704, 828, 1505
AsuC2I CCSGG 1 cut(s) 1271
AsuHPI GGTGA 9 cut(s) 538, 653, 674, 898, 1203, 1209, 1221, 1254, 1630
AvaII GGWCC 4 cut(s) 156, 242, 704, 1505
BanI GGYRCC 1 cut(s) 1246
BanII GRGCYC 1 cut(s) 1007
BbsI GAAGAC 2 cut(s) 303, 570
BbvI GCAGC 2 cut(s) 74, 1507
BccI CCATC 6 cut(s) 347, 557, 659, 967, 1222, 1302
BceAI ACGGC 3 cut(s) 1010, 1039, 1638
BciT130I CCWGG 2 cut(s) 20, 708
BciVI GTATCC 1 cut(s) 753
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 1271
BcoDI GTCTC 5 cut(s) 622, 1020, 1095, 1173, 1470
BfaI CTAG 3 cut(s) 194, 683, 734
BfmI CTRYAG 1 cut(s) 1039
BfuI GTATCC 1 cut(s) 753
BisI GCNGC 3 cut(s) 63, 1313, 1521
BlsI GCNGC 3 cut(s) 64, 1314, 1522
Bme1390I CCNGG 3 cut(s) 20, 708, 1271
Bme18I GGWCC 4 cut(s) 156, 242, 704, 1505
BmgBI CACGTC 2 cut(s) 982, 1122
BmgT120I GGNCC 5 cut(s) 156, 242, 704, 828, 1505
BmiI GGNNCC 5 cut(s) 291, 305, 957, 1248, 1275
BmrFI CCNGG 3 cut(s) 20, 708, 1271
BmsI GCATC 2 cut(s) 59, 151
BoxI GACNNNNGTC 1 cut(s) 1041
BpiI GAAGAC 2 cut(s) 303, 570
BpmI CTGGAG 3 cut(s) 276, 505, 1326
Bpu10I CCTNAGC 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 1271
BsaAI YACGTR 1 cut(s) 1190
BsaBI GATNNNNATC 2 cut(s) 14, 1083
BsaJI CCNNGG 2 cut(s) 274, 1269
BsaWI WCCGGW 4 cut(s) 300, 638, 1018, 1276
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 7 cut(s) 713, 764, 845, 1205, 1243, 1276, 1430
Bse118I RCCGGY 5 cut(s) 596, 638, 1154, 1243, 1523
Bse1I ACTGG 5 cut(s) 208, 758, 799, 1250, 1370
Bse3DI GCAATG 2 cut(s) 256, 630
Bse8I GATNNNNATC 2 cut(s) 14, 1083
BseBI CCWGG 2 cut(s) 20, 708
BseDI CCNNGG 2 cut(s) 274, 1269
BseGI GGATG 4 cut(s) 15, 959, 1294, 1511
BseJI GATNNNNATC 2 cut(s) 14, 1083
BseLI CCNNNNNNNGG 7 cut(s) 713, 764, 845, 1205, 1243, 1276, 1430
BseMI GCAATG 2 cut(s) 256, 630
BseNI ACTGG 5 cut(s) 208, 758, 799, 1250, 1370
BseRI GAGGAG 5 cut(s) 1293, 1323, 1350, 1377, 1404
BseXI GCAGC 2 cut(s) 74, 1507
BsgI GTGCAG 2 cut(s) 769, 1624
Bsh1285I CGRYCG 1 cut(s) 1023
BshFI GGCC 1 cut(s) 829
BshNI GGYRCC 1 cut(s) 1246
BshTI ACCGGT 1 cut(s) 638
BsiEI CGRYCG 1 cut(s) 1023
BslI CCNNNNNNNGG 7 cut(s) 713, 764, 845, 1205, 1243, 1276, 1430
BsmAI GTCTC 5 cut(s) 622, 1020, 1095, 1173, 1470
BsmBI CGTCTC 2 cut(s) 1095, 1470
BsnI GGCC 1 cut(s) 829
Bsp1286I GDGCHC 1 cut(s) 1007
Bsp143I GATC 4 cut(s) 70, 609, 1084, 1459
BspANI GGCC 1 cut(s) 829
BspLI GGNNCC 5 cut(s) 291, 305, 957, 1248, 1275
BspPI GGATC 1 cut(s) 1454
BspT107I GGYRCC 1 cut(s) 1246
BsrDI GCAATG 2 cut(s) 256, 630
BsrFI RCCGGY 5 cut(s) 596, 638, 1154, 1243, 1523
BsrI ACTGG 5 cut(s) 208, 758, 799, 1250, 1370
BssAI RCCGGY 5 cut(s) 596, 638, 1154, 1243, 1523
BssECI CCNNGG 2 cut(s) 274, 1269
BssMI GATC 4 cut(s) 70, 609, 1084, 1459
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 2 cut(s) 20, 708
Bst6I CTCTTC 4 cut(s) 1341, 1368, 1395, 1422
BstBAI YACGTR 1 cut(s) 1190
BstC8I GCNNGC 6 cut(s) 288, 1052, 1317, 1398, 1425, 1525
BstDEI CTNAG 2 cut(s) 855, 1164
BstEII GGTNACC 1 cut(s) 544
BstF5I GGATG 4 cut(s) 15, 959, 1294, 1511
BstHHI GCGC 1 cut(s) 671
BstKTI GATC 4 cut(s) 73, 612, 1087, 1462
BstMAI GTCTC 5 cut(s) 622, 1020, 1095, 1173, 1470
BstMBI GATC 4 cut(s) 70, 609, 1084, 1459
BstMCI CGRYCG 1 cut(s) 1023
BstMWI GCNNNNNNNGC 3 cut(s) 933, 1280, 1622
BstNI CCWGG 2 cut(s) 20, 708
BstNSI RCATGY 3 cut(s) 168, 1054, 1179
BstPAI GACNNNNGTC 1 cut(s) 1041
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 3 cut(s) 18, 706, 1269
BstSFI CTRYAG 1 cut(s) 1039
BstV1I GCAGC 2 cut(s) 74, 1507
BstV2I GAAGAC 2 cut(s) 303, 570
BsuI GTATCC 1 cut(s) 753
BsuRI GGCC 1 cut(s) 829
BtgZI GCGATG 1 cut(s) 577
BtrI CACGTC 2 cut(s) 982, 1122
BtsCI GGATG 4 cut(s) 15, 959, 1294, 1511
BtsI GCAGTG 2 cut(s) 1407, 1434
BtsIMutI CAGTG 6 cut(s) 496, 576, 765, 982, 1407, 1434
Cac8I GCNNGC 6 cut(s) 288, 1052, 1317, 1398, 1425, 1525
CfoI GCGC 1 cut(s) 671
Cfr10I RCCGGY 5 cut(s) 596, 638, 1154, 1243, 1523
Cfr13I GGNCC 5 cut(s) 156, 242, 704, 828, 1505
Csp6I GTAC 2 cut(s) 186, 1253
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 9 cut(s) 165, 246, 515, 713, 825, 883, 1051, 1176, 1457
CviQI GTAC 2 cut(s) 186, 1253
DdeI CTNAG 2 cut(s) 855, 1164
DpnI GATC 4 cut(s) 72, 611, 1086, 1461
DpnII GATC 4 cut(s) 70, 609, 1084, 1459
Eam1104I CTCTTC 4 cut(s) 1341, 1368, 1395, 1422
EarI CTCTTC 4 cut(s) 1341, 1368, 1395, 1422
EciI GGCGGA 4 cut(s) 1356, 1383, 1410, 1458
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 1007
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 4 cut(s) 156, 242, 704, 1505
Eco91I GGTNACC 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 544
EcoRII CCWGG 2 cut(s) 18, 706
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 1007
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 2 cut(s) 1095, 1470
FaeI CATG 9 cut(s) 168, 249, 518, 716, 828, 886, 1054, 1179, 1460
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 9 cut(s) 164, 245, 514, 712, 824, 882, 1050, 1175, 1456
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 2 cut(s) 179, 1223
Fnu4HI GCNGC 3 cut(s) 63, 1313, 1521
FokI GGATG 4 cut(s) 2, 946, 1281, 1498
FriOI GRGCYC 1 cut(s) 1007
Fsp4HI GCNGC 3 cut(s) 63, 1313, 1521
FspBI CTAG 3 cut(s) 194, 683, 734
GlaI GCGC 1 cut(s) 670
GluI GCNGC 3 cut(s) 63, 1313, 1521
GsuI CTGGAG 3 cut(s) 276, 505, 1326
HaeIII GGCC 1 cut(s) 829
HhaI GCGC 1 cut(s) 671
Hin1II CATG 9 cut(s) 168, 249, 518, 716, 828, 886, 1054, 1179, 1460
Hin6I GCGC 1 cut(s) 669
HinP1I GCGC 1 cut(s) 669
HincII GTYRAC 4 cut(s) 144, 180, 454, 1224
HindII GTYRAC 4 cut(s) 144, 180, 454, 1224
HinfI GANTC 8 cut(s) 122, 170, 317, 585, 623, 816, 833, 987
HpaI GTTAAC 1 cut(s) 144
HphI GGTGA 9 cut(s) 538, 653, 674, 898, 1203, 1209, 1221, 1254, 1630
Hpy166II GTNNAC 7 cut(s) 144, 156, 180, 328, 454, 1211, 1224
Hpy188I TCNGA 6 cut(s) 127, 622, 1047, 1062, 1078, 1126
Hpy188III TCNNGA 5 cut(s) 293, 746, 778, 820, 991
Hpy8I GTNNAC 7 cut(s) 144, 156, 180, 328, 454, 1211, 1224
Hpy99I CGWCG 5 cut(s) 184, 380, 434, 1225, 1500
HpyAV CCTTC 3 cut(s) 233, 839, 1488
HpyCH4IV ACGT 4 cut(s) 533, 981, 1121, 1189
HpyF10VI GCNNNNNNNGC 3 cut(s) 933, 1280, 1622
HpyF3I CTNAG 2 cut(s) 855, 1164
HpySE526I ACGT 4 cut(s) 533, 981, 1121, 1189
Hsp92II CATG 9 cut(s) 168, 249, 518, 716, 828, 886, 1054, 1179, 1460
HspAI GCGC 1 cut(s) 669
KroI GCCGGC 1 cut(s) 1523
KroNI GCCGGC 1 cut(s) 1525
Ksp22I TGATCA 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 144
Kzo9I GATC 4 cut(s) 70, 609, 1084, 1459
LmnI GCTCC 7 cut(s) 259, 276, 295, 303, 809, 1069, 1279
Lsp1109I GCAGC 2 cut(s) 74, 1507
LweI GCATC 2 cut(s) 59, 151
MaeI CTAG 3 cut(s) 194, 683, 734
MaeII ACGT 4 cut(s) 533, 981, 1121, 1189
MalI GATC 4 cut(s) 72, 611, 1086, 1461
MboI GATC 4 cut(s) 70, 609, 1084, 1459
MboII GAAGA 7 cut(s) 308, 575, 907, 1328, 1355, 1382, 1409
MfeI CAATTG 1 cut(s) 657
MhlI GDGCHC 1 cut(s) 1007
MluCI AATT 6 cut(s) 263, 457, 497, 657, 1149, 1534
MlyI GAGTC 3 cut(s) 116, 311, 617
MmeI TCCRAC 3 cut(s) 224, 645, 714
Mph1103I ATGCAT 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 1523
MseI TTAA 3 cut(s) 143, 333, 1140
MslI CAYNNNNRTG 3 cut(s) 507, 881, 884
MspA1I CMGCKG 2 cut(s) 65, 1520
MspR9I CCNGG 3 cut(s) 20, 708, 1271
MunI CAATTG 1 cut(s) 657
MvaI CCWGG 2 cut(s) 20, 708
MwoI GCNNNNNNNGC 3 cut(s) 933, 1280, 1622
NaeI GCCGGC 1 cut(s) 1525
NciI CCSGG 1 cut(s) 1271
NdeII GATC 4 cut(s) 70, 609, 1084, 1459
NgoMIV GCCGGC 1 cut(s) 1523
NlaIII CATG 9 cut(s) 168, 249, 518, 716, 828, 886, 1054, 1179, 1460
NlaIV GGNNCC 5 cut(s) 291, 305, 957, 1248, 1275
NmuCI GTSAC 9 cut(s) 91, 544, 641, 769, 977, 1159, 1190, 1260, 1618
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 3 cut(s) 168, 1054, 1179
OliI CACNNNNGTG 1 cut(s) 507
PaeI GCATGC 1 cut(s) 1054
PcsI WCGNNNNNNNCGW 1 cut(s) 1110
PdiI GCCGGC 1 cut(s) 1525
PfeI GAWTC 5 cut(s) 170, 585, 816, 833, 987
PflFI GACNNNGTC 1 cut(s) 1165
PflMI CCANNNNNTGG 1 cut(s) 713
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 3 cut(s) 64, 1314, 1522
PleI GAGTC 3 cut(s) 116, 311, 617
PpsI GAGTC 3 cut(s) 116, 311, 617
Ppu21I YACGTR 1 cut(s) 1190
PshAI GACNNNNGTC 1 cut(s) 1041
Psp6I CCWGG 2 cut(s) 18, 706
PspEI GGTNACC 1 cut(s) 544
PspGI CCWGG 2 cut(s) 18, 706
PspN4I GGNNCC 5 cut(s) 291, 305, 957, 1248, 1275
PspPI GGNCC 5 cut(s) 156, 242, 704, 828, 1505
PsyI GACNNNGTC 1 cut(s) 1165
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 187, 1254
RsaNI GTAC 2 cut(s) 186, 1253
RseI CAYNNNNRTG 3 cut(s) 507, 881, 884
SalI GTCGAC 2 cut(s) 178, 1222
SaqAI TTAA 3 cut(s) 143, 333, 1140
SatI GCNGC 3 cut(s) 63, 1313, 1521
Sau3AI GATC 4 cut(s) 70, 609, 1084, 1459
Sau96I GGNCC 5 cut(s) 156, 242, 704, 828, 1505
SchI GAGTC 3 cut(s) 116, 311, 617
ScrFI CCNGG 3 cut(s) 20, 708, 1271
SduI GDGCHC 1 cut(s) 1007
SfaNI GCATC 2 cut(s) 59, 151
SfcI CTRYAG 1 cut(s) 1039
SinI GGWCC 4 cut(s) 156, 242, 704, 1505
SmiMI CAYNNNNRTG 3 cut(s) 507, 881, 884
SphI GCATGC 1 cut(s) 1054
Sse9I AATT 6 cut(s) 263, 457, 497, 657, 1149, 1534
SspMI CTAG 3 cut(s) 194, 683, 734
StyD4I CCNGG 3 cut(s) 18, 706, 1269
StyI CCWWGG 1 cut(s) 274
TaiI ACGT 4 cut(s) 536, 984, 1124, 1192
TaqI TCGA 9 cut(s) 8, 101, 179, 342, 375, 432, 602, 1035, 1223
TaqII GACCGA 1 cut(s) 173
TasI AATT 6 cut(s) 263, 457, 497, 657, 1149, 1534
TatI WGTACW 1 cut(s) 1252
TauI GCSGC 1 cut(s) 1315
TfiI GAWTC 5 cut(s) 170, 585, 816, 833, 987
Tru1I TTAA 3 cut(s) 143, 333, 1140
Tru9I TTAA 3 cut(s) 143, 333, 1140
TscAI CASTG 6 cut(s) 496, 583, 765, 982, 1407, 1434
TseFI GTSAC 9 cut(s) 91, 544, 641, 769, 977, 1159, 1190, 1260, 1618
TseI GCWGC 2 cut(s) 62, 1520
Tsp45I GTSAC 9 cut(s) 91, 544, 641, 769, 977, 1159, 1190, 1260, 1618
TspDTI ATGAA 4 cut(s) 17, 26, 503, 765
TspGWI ACGGA 5 cut(s) 393, 597, 822, 1452, 1479
TspRI CASTG 6 cut(s) 496, 583, 765, 982, 1407, 1434
Tth111I GACNNNGTC 1 cut(s) 1165
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 4 cut(s) 156, 242, 704, 1505
XapI RAATTY 1 cut(s) 497
XceI RCATGY 3 cut(s) 168, 1054, 1179
XmiI GTMKAC 2 cut(s) 179, 1223
XspI CTAG 3 cut(s) 194, 683, 734
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.