Rroxscaffold_7G00175000

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011526
Physical Location & Seq
Forward (+)
14694863 .. 14696833
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175000.1

Sequence Viewer

Length: 1971 bp
ATGGGTCTGAAATCGATAAACATTTTGGCAATACTTTTGCTCTCATTTTTAGCATCCACCGCTGTAGCTGATCAATCCAATGTCTTCGATGTGACAAGCTCAAAATATGGTGGAAAGCCCAACTCGGATATTTCAAGGGCATTGACAATGGCATGGACAGATGCGTGTGCATCAACGTCAACGAGTACTGTCCTTGTTCCGAGGGGAACATACAAGTTATTACAAGAAGCAAAATTTGAAGGTCCTTGCAAGGAGCCTTTTCAGTTTCAGCTCCAAGGAACATTGCAGGCTCCGAAAGACCGGAGGCAACTCGATGACTCTTGGATTTCCTTTAACAACATCGACATGCTCACCTTGTCCGGCGGTGGAACTATCGACGGACAAGGAGCCCTTTCTTGGAAGGAGAATCATGACTCTACTCCCGTTGATATTAGGTTCCAAACTGTCAACAATTCCACAATTAGCGACATAGCTTCAATCGACAGCGCAAAGTTCCACATTAGTGTTCATGGCTGCAACAACGTTACGTTCCAACGGCTCACCATCACAGCACCCGAAGACAGTCCTAACACCGACGGAATCCACATCTTTAGTTCGACCAAGATCACAGTCGCCGACTCCTACATTGCAACCGGTGACGATTGTGTTTCAATTGGCGATGGCTCCAACCAAATCTTGGTGTCCAACATTACTTGCGGACCAGGTCATGGAATAAGCATAGGCAGTCTAGGAAAGTATACAGACGAACAGCCGGTGGTCGGCGTCACGGTCAAGAACTGCACGCTTACCGGCACAAGCAACGGCGCGAGAATCAAGACATGGCCCGATTCTCCGGCAACAAGCGTTGCTCGGAATATACTCTTCCAAGACATGGTCATGGTGAATGTGAAGAACCCTATCCTCATCGACCAGAACTACTGTGACAAAGGCAAGTCGTGTAAGCTAATGATTCCATCCAAAGTCCAGATCAGTAACGTGACATTCAAGGATATCAGAGGCTCGTCTTCGTCTCCGGTTGCCGTCAAGCTCGACTGTAGTCACAGCGTGCCGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCAGTTACAGTGGAGACAAAAAAGACGAAATCACGTCTGTGTGTAATAATGTTAAGCCGAGAATTACTGGCGTGACTAAGGCTCTTGCTTGTGCCAATCTAGGTGACATACCAATAGATAATTCACCGCCGCCATCGACTTCACCGCCTCTTCCACCGCCGCTGCCGGCTTCACCTCCTCTTCCACCGCCGCTGCTGGCTTCACCTCCTCTTCCACCGCCGCTGCCGGCTTCACCTCCTCTTCTACCGGTACCAGTACAACAGTCACCACCGGTACCAGTACAACAGTCACTACCCCCGCCTCCGGTAGAAAAACACCATTCCCCTTCTCCGCCACCATTTTCGCCTCCTCTTTCACCGCCGGCGCCAATTGCTTCACCTTCACCGCCGCCATATTTACCTCCTTTTTTGCCGCCGCCATTTGTTTTACCTTCTCCACCACCATCTTCACCTCCTCTTTCGCCGTTGGAGCCATTTATTTCACCTTCACCGCTACCATCTTTACCTCCTCCTTTGCCGCCGTCATTCGTTTTACCTTCTCCGCCACCATCTTCACCTCCTCTTTCGCCGCCGGCGCCATTTGTTTTACCTCCACCGCCACCATCTTTACCTCTTCTTTTGCCGCCTCCATTAGTTTCACCTTCTCTTTCGCCGCCACCATTCGTATCACCTCCTCTTCCGCCGATGGTGGACACACCTCCTCACCCTACGGTAGCATCAGACGATTCGTCTCCGCCGATAATAATGGTGTATGGGTATCCATATCCTCTGTCTCATACTGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGGCAAAAACCTACAGTCAGAGTGATGGTGCTTAGTTCCGCCCTGGCTTTGTGGTTGCTGCAGGCCTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

656

Amino Acids

68.96

Weight (kDa)

5.57

Isoelectric Point (pI)

74.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 375 9.2e-98 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1330, 1354
AccB1I GGYRCC 4 cut(s) 1330, 1354, 1444, 1654
AccB7I CCANNNNNTGG 3 cut(s) 676, 707, 871
AccI GTMKAC 1 cut(s) 737
AccII CGCG 1 cut(s) 806
AclI AACGTT 1 cut(s) 522
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 3 cut(s) 762, 1445, 1655
AdeI CACNNNGTG 1 cut(s) 1045
AfaI GTAC 5 cut(s) 187, 1332, 1338, 1356, 1362
AfiI CCNNNNNNNGG 8 cut(s) 396, 676, 707, 758, 871, 1060, 1384, 1896
AgeI ACCGGT 3 cut(s) 632, 1327, 1351
AgsI TTSAA 5 cut(s) 135, 239, 477, 651, 985
AhdI GACNNNNNGTC 1 cut(s) 1807
AjiI CACGTC 1 cut(s) 1116
AjnI CCWGG 2 cut(s) 700, 1938
AjuI GAANNNNNNNTTGG 2 cut(s) 1186, 1218
AleI CACNNNNGTG 2 cut(s) 501, 1118
AluBI AGCT 7 cut(s) 68, 99, 271, 473, 943, 1027, 1066
AluI AGCT 7 cut(s) 68, 99, 271, 473, 943, 1027, 1066
Alw26I GTCTC 4 cut(s) 1014, 1089, 1815, 1857
AoxI GGCC 2 cut(s) 821, 1959
ApeKI GCWGC 5 cut(s) 513, 1243, 1273, 1303, 1954
ApoI RAATTY 1 cut(s) 233
AsiGI ACCGGT 3 cut(s) 632, 1327, 1351
Asp718I GGTACC 2 cut(s) 1330, 1354
AspLEI GCGC 4 cut(s) 488, 806, 1447, 1657
AspS9I GGNCC 4 cut(s) 242, 698, 822, 1877
AsuC2I CCSGG 1 cut(s) 1897
AvaII GGWCC 3 cut(s) 242, 698, 1877
BanI GGYRCC 4 cut(s) 1330, 1354, 1444, 1654
BanII GRGCYC 1 cut(s) 391
BbsI GAAGAC 3 cut(s) 76, 564, 996
BbvI GCAGC 5 cut(s) 500, 1230, 1260, 1290, 1941
BceAI ACGGC 6 cut(s) 551, 817, 1004, 1033, 1528, 1585
BciT130I CCWGG 2 cut(s) 702, 1940
BciVI GTATCC 1 cut(s) 1848
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 1897
BcoDI GTCTC 4 cut(s) 1014, 1089, 1815, 1857
BfaI CTAG 2 cut(s) 728, 1181
BfmI CTRYAG 4 cut(s) 63, 1033, 1908, 1955
BfoI RGCGCY 2 cut(s) 1448, 1658
BfuI GTATCC 1 cut(s) 1848
BmcAI AGTACT 1 cut(s) 187
Bme1390I CCNGG 3 cut(s) 702, 1897, 1940
Bme18I GGWCC 3 cut(s) 242, 698, 1877
BmeRI GACNNNNNGTC 1 cut(s) 1807
BmgBI CACGTC 1 cut(s) 1116
BmgT120I GGNCC 4 cut(s) 242, 698, 822, 1877
BmrFI CCNGG 3 cut(s) 702, 1897, 1940
BmsI GCATC 4 cut(s) 62, 151, 179, 1805
BoxI GACNNNNGTC 1 cut(s) 1035
BpiI GAAGAC 3 cut(s) 76, 564, 996
BpuMI CCSGG 1 cut(s) 1897
Bsa29I ATCGAT 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 1077
BsaHI GRCGYC 3 cut(s) 762, 1445, 1655
BsaJI CCNNGG 3 cut(s) 200, 274, 1938
BsaWI WCCGGW 6 cut(s) 300, 632, 1012, 1327, 1351, 1384
Bsc4I CCNNNNNNNGG 8 cut(s) 396, 676, 707, 758, 871, 1060, 1384, 1896
Bse118I RCCGGY 9 cut(s) 632, 751, 788, 1246, 1306, 1327, 1351, 1441, 1651
Bse1I ACTGG 3 cut(s) 1153, 1334, 1358
Bse3DI GCAATG 2 cut(s) 281, 624
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 2 cut(s) 702, 1940
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 3 cut(s) 200, 274, 1938
BseGI GGATG 3 cut(s) 53, 953, 1883
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 8 cut(s) 396, 676, 707, 758, 871, 1060, 1384, 1896
BseMI GCAATG 2 cut(s) 281, 624
BseMII CTCAG 1 cut(s) 1096
BseNI ACTGG 3 cut(s) 1153, 1334, 1358
BseRI GAGGAG 9 cut(s) 1248, 1278, 1308, 1419, 1524, 1578, 1629, 1743, 1770
BseXI GCAGC 5 cut(s) 500, 1230, 1260, 1290, 1941
BsgI GTGCAG 1 cut(s) 763
Bsh1236I CGCG 1 cut(s) 806
BshFI GGCC 2 cut(s) 823, 1961
BshNI GGYRCC 4 cut(s) 1330, 1354, 1444, 1654
BshTI ACCGGT 3 cut(s) 632, 1327, 1351
BshVI ATCGAT 1 cut(s) 14
BslI CCNNNNNNNGG 8 cut(s) 396, 676, 707, 758, 871, 1060, 1384, 1896
BsmAI GTCTC 4 cut(s) 1014, 1089, 1815, 1857
BsmBI CGTCTC 2 cut(s) 1014, 1815
BsnI GGCC 2 cut(s) 823, 1961
Bsp1286I GDGCHC 1 cut(s) 391
Bsp143I GATC 4 cut(s) 70, 603, 966, 1078
BspANI GGCC 2 cut(s) 823, 1961
BspCNI CTCAG 1 cut(s) 1095
BspDI ATCGAT 1 cut(s) 14
BspFNI CGCG 1 cut(s) 806
BspHI TCATGA 1 cut(s) 409
BspMAI CTGCAG 1 cut(s) 1959
BspT107I GGYRCC 4 cut(s) 1330, 1354, 1444, 1654
BsrDI GCAATG 2 cut(s) 281, 624
BsrFI RCCGGY 9 cut(s) 632, 751, 788, 1246, 1306, 1327, 1351, 1441, 1651
BsrI ACTGG 3 cut(s) 1153, 1334, 1358
BssAI RCCGGY 9 cut(s) 632, 751, 788, 1246, 1306, 1327, 1351, 1441, 1651
BssECI CCNNGG 3 cut(s) 200, 274, 1938
BssMI GATC 4 cut(s) 70, 603, 966, 1078
BssNAI GTATAC 1 cut(s) 738
BssNI GRCGYC 3 cut(s) 762, 1445, 1655
BssT1I CCWWGG 1 cut(s) 274
Bst1107I GTATAC 1 cut(s) 738
Bst2UI CCWGG 2 cut(s) 702, 1940
Bst6I CTCTTC 7 cut(s) 866, 1236, 1266, 1296, 1326, 1698, 1761
BstACI GRCGYC 3 cut(s) 762, 1445, 1655
BstC8I GCNNGC 9 cut(s) 288, 782, 1046, 1248, 1278, 1308, 1443, 1653, 1959
BstDEI CTNAG 3 cut(s) 1082, 1158, 1928
BstF5I GGATG 3 cut(s) 53, 953, 1883
BstFNI CGCG 1 cut(s) 806
BstH2I RGCGCY 2 cut(s) 1448, 1658
BstHHI GCGC 4 cut(s) 488, 806, 1447, 1657
BstKTI GATC 4 cut(s) 73, 606, 969, 1081
BstMAI GTCTC 4 cut(s) 1014, 1089, 1815, 1857
BstMBI GATC 4 cut(s) 70, 603, 966, 1078
BstMWI GCNNNNNNNGC 5 cut(s) 59, 1050, 1451, 1549, 1654
BstNI CCWGG 2 cut(s) 702, 1940
BstNSI RCATGY 1 cut(s) 349
BstPAI GACNNNNGTC 1 cut(s) 1035
BstSCI CCNGG 3 cut(s) 700, 1895, 1938
BstSFI CTRYAG 4 cut(s) 63, 1033, 1908, 1955
BstUI CGCG 1 cut(s) 806
BstV1I GCAGC 5 cut(s) 500, 1230, 1260, 1290, 1941
BstV2I GAAGAC 3 cut(s) 76, 564, 996
BstZ17I GTATAC 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 14
BsuI GTATCC 1 cut(s) 1848
BsuRI GGCC 2 cut(s) 823, 1961
BsuTUI ATCGAT 1 cut(s) 14
BtgZI GCGATG 1 cut(s) 672
BtrI CACGTC 1 cut(s) 1116
BtsCI GGATG 3 cut(s) 53, 953, 1883
BtsIMutI CAGTG 1 cut(s) 1096
Cac8I GCNNGC 9 cut(s) 288, 782, 1046, 1248, 1278, 1308, 1443, 1653, 1959
CciI TCATGA 1 cut(s) 409
CfoI GCGC 4 cut(s) 488, 806, 1447, 1657
Cfr10I RCCGGY 9 cut(s) 632, 751, 788, 1246, 1306, 1327, 1351, 1441, 1651
Cfr13I GGNCC 4 cut(s) 242, 698, 822, 1877
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 1 cut(s) 751
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 5 cut(s) 186, 1331, 1337, 1355, 1361
CspAI ACCGGT 3 cut(s) 632, 1327, 1351
CviAII CATG 8 cut(s) 153, 346, 410, 509, 707, 819, 871, 877
CviQI GTAC 5 cut(s) 186, 1331, 1337, 1355, 1361
DdeI CTNAG 3 cut(s) 1082, 1158, 1928
DinI GGCGCC 2 cut(s) 1446, 1656
DpnI GATC 4 cut(s) 72, 605, 968, 1080
DpnII GATC 4 cut(s) 70, 603, 966, 1078
DraIII CACNNNGTG 1 cut(s) 1045
DriI GACNNNNNGTC 1 cut(s) 1807
Eam1104I CTCTTC 7 cut(s) 866, 1236, 1266, 1296, 1326, 1698, 1761
Eam1105I GACNNNNNGTC 1 cut(s) 1807
EarI CTCTTC 7 cut(s) 866, 1236, 1266, 1296, 1326, 1698, 1761
EciI GGCGGA 6 cut(s) 1401, 1611, 1749, 1803, 1879, 1924
Eco130I CCWWGG 1 cut(s) 274
Eco147I AGGCCT 1 cut(s) 1961
Eco24I GRGCYC 1 cut(s) 391
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 3 cut(s) 242, 698, 1877
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 700, 1938
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 391
EgeI GGCGCC 2 cut(s) 1446, 1656
EheI GGCGCC 2 cut(s) 1446, 1656
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 2 cut(s) 1014, 1815
FaeI CATG 8 cut(s) 156, 349, 413, 512, 710, 822, 874, 880
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 8 cut(s) 152, 345, 409, 508, 706, 818, 870, 876
FauI CCCGC 1 cut(s) 1386
FbaI TGATCA 1 cut(s) 70
FblI GTMKAC 1 cut(s) 737
FokI GGATG 3 cut(s) 40, 940, 1870
FriOI GRGCYC 1 cut(s) 391
FspBI CTAG 2 cut(s) 728, 1181
GlaI GCGC 4 cut(s) 487, 805, 1446, 1656
HaeII RGCGCY 2 cut(s) 1448, 1658
HaeIII GGCC 2 cut(s) 823, 1961
HgaI GACGC 1 cut(s) 751
HhaI GCGC 4 cut(s) 488, 806, 1447, 1657
Hin1I GRCGYC 3 cut(s) 762, 1445, 1655
Hin1II CATG 8 cut(s) 156, 349, 413, 512, 710, 822, 874, 880
Hin6I GCGC 4 cut(s) 486, 804, 1445, 1655
HinP1I GCGC 4 cut(s) 486, 804, 1445, 1655
HincII GTYRAC 2 cut(s) 180, 448
HindII GTYRAC 2 cut(s) 180, 448
HinfI GANTC 9 cut(s) 317, 406, 413, 579, 617, 810, 827, 949, 1805
Hpy166II GTNNAC 4 cut(s) 180, 448, 738, 1771
Hpy188I TCNGA 9 cut(s) 9, 127, 201, 294, 852, 995, 1072, 1801, 1916
Hpy188III TCNNGA 4 cut(s) 410, 772, 814, 964
Hpy8I GTNNAC 4 cut(s) 180, 448, 738, 1771
Hpy99I CGWCG 2 cut(s) 380, 578
HpyAV CCTTC 8 cut(s) 233, 394, 1416, 1470, 1521, 1575, 1626, 1731
HpyCH4IV ACGT 5 cut(s) 176, 522, 527, 975, 1115
HpyCH4V TGCA 7 cut(s) 170, 249, 286, 516, 629, 780, 1957
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 1050, 1451, 1549, 1654
HpyF3I CTNAG 3 cut(s) 1082, 1158, 1928
HpySE526I ACGT 5 cut(s) 176, 522, 527, 975, 1115
Hsp92I GRCGYC 3 cut(s) 762, 1445, 1655
Hsp92II CATG 8 cut(s) 156, 349, 413, 512, 710, 822, 874, 880
HspAI GCGC 4 cut(s) 486, 804, 1445, 1655
KasI GGCGCC 2 cut(s) 1444, 1654
KpnI GGTACC 2 cut(s) 1334, 1358
KroI GCCGGC 4 cut(s) 1246, 1306, 1441, 1651
KroNI GCCGGC 4 cut(s) 1248, 1308, 1443, 1653
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 4 cut(s) 70, 603, 966, 1078
LmnI GCTCC 7 cut(s) 253, 276, 295, 386, 668, 1063, 1549
Lsp1109I GCAGC 5 cut(s) 500, 1230, 1260, 1290, 1941
LweI GCATC 4 cut(s) 62, 151, 179, 1805
MabI ACCWGGT 1 cut(s) 700
MaeI CTAG 2 cut(s) 728, 1181
MaeII ACGT 5 cut(s) 176, 522, 527, 975, 1115
MalI GATC 4 cut(s) 72, 605, 968, 1080
MboI GATC 4 cut(s) 70, 603, 966, 1078
MfeI CAATTG 2 cut(s) 651, 1449
MhlI GDGCHC 1 cut(s) 391
MluCI AATT 7 cut(s) 233, 451, 459, 651, 1143, 1201, 1449
Mly113I GGCGCC 2 cut(s) 1445, 1655
MlyI GAGTC 3 cut(s) 311, 407, 611
MmeI TCCRAC 4 cut(s) 556, 690, 708, 1527
MreI CGCCGGCG 2 cut(s) 1441, 1651
MroNI GCCGGC 4 cut(s) 1246, 1306, 1441, 1651
MseI TTAA 2 cut(s) 333, 1134
MslI CAYNNNNRTG 5 cut(s) 344, 501, 875, 1118, 1860
MspA1I CMGCKG 4 cut(s) 62, 1243, 1273, 1303
MspR9I CCNGG 3 cut(s) 702, 1897, 1940
MunI CAATTG 2 cut(s) 651, 1449
MvaI CCWGG 2 cut(s) 702, 1940
MvnI CGCG 1 cut(s) 806
MwoI GCNNNNNNNGC 5 cut(s) 59, 1050, 1451, 1549, 1654
NaeI GCCGGC 4 cut(s) 1248, 1308, 1443, 1653
NarI GGCGCC 2 cut(s) 1445, 1655
NciI CCSGG 1 cut(s) 1897
NdeII GATC 4 cut(s) 70, 603, 966, 1078
NgoMIV GCCGGC 4 cut(s) 1246, 1306, 1441, 1651
NlaIII CATG 8 cut(s) 156, 349, 413, 512, 710, 822, 874, 880
NmeAIII GCCGAG 1 cut(s) 1164
NspI RCATGY 1 cut(s) 349
OliI CACNNNNGTG 2 cut(s) 501, 1118
PagI TCATGA 1 cut(s) 409
PceI AGGCCT 1 cut(s) 1961
PcsI WCGNNNNNNNCGW 1 cut(s) 1814
PdiI GCCGGC 4 cut(s) 1248, 1308, 1443, 1653
PfeI GAWTC 6 cut(s) 406, 579, 810, 827, 949, 1805
PflFI GACNNNGTC 2 cut(s) 702, 872
PflMI CCANNNNNTGG 3 cut(s) 676, 707, 871
PinAI ACCGGT 3 cut(s) 632, 1327, 1351
PleI GAGTC 3 cut(s) 311, 407, 611
PluTI GGCGCC 2 cut(s) 1448, 1658
PpsI GAGTC 3 cut(s) 311, 407, 611
PpuMI RGGWCCY 1 cut(s) 242
PshAI GACNNNNGTC 1 cut(s) 1035
Psp1406I AACGTT 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 700, 1938
PspGI CCWGG 2 cut(s) 700, 1938
PspPI GGNCC 4 cut(s) 242, 698, 822, 1877
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 1959
PsyI GACNNNGTC 2 cut(s) 702, 872
RsaI GTAC 5 cut(s) 187, 1332, 1338, 1356, 1362
RsaNI GTAC 5 cut(s) 186, 1331, 1337, 1355, 1361
RseI CAYNNNNRTG 5 cut(s) 344, 501, 875, 1118, 1860
SaqAI TTAA 2 cut(s) 333, 1134
Sau3AI GATC 4 cut(s) 70, 603, 966, 1078
Sau96I GGNCC 4 cut(s) 242, 698, 822, 1877
ScaI AGTACT 1 cut(s) 187
SchI GAGTC 3 cut(s) 311, 407, 611
ScrFI CCNGG 3 cut(s) 702, 1897, 1940
SduI GDGCHC 1 cut(s) 391
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 4 cut(s) 62, 151, 179, 1805
SfcI CTRYAG 4 cut(s) 63, 1033, 1908, 1955
SfoI GGCGCC 2 cut(s) 1446, 1656
SgrAI CRCCGGYG 2 cut(s) 1441, 1651
SinI GGWCC 3 cut(s) 242, 698, 1877
SmiMI CAYNNNNRTG 5 cut(s) 344, 501, 875, 1118, 1860
Sse9I AATT 7 cut(s) 233, 451, 459, 651, 1143, 1201, 1449
SseBI AGGCCT 1 cut(s) 1961
SspDI GGCGCC 2 cut(s) 1444, 1654
SspMI CTAG 2 cut(s) 728, 1181
StuI AGGCCT 1 cut(s) 1961
StyD4I CCNGG 3 cut(s) 700, 1895, 1938
StyI CCWWGG 1 cut(s) 274
TaiI ACGT 5 cut(s) 179, 525, 530, 978, 1118
TasI AATT 7 cut(s) 233, 451, 459, 651, 1143, 1201, 1449
TatI WGTACW 3 cut(s) 185, 1336, 1360
TfiI GAWTC 6 cut(s) 406, 579, 810, 827, 949, 1805
Tru1I TTAA 2 cut(s) 333, 1134
Tru9I TTAA 2 cut(s) 333, 1134
TscAI CASTG 1 cut(s) 1096
TseI GCWGC 5 cut(s) 513, 1243, 1273, 1303, 1954
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 2 cut(s) 393, 591
TspRI CASTG 1 cut(s) 1096
Tth111I GACNNNGTC 2 cut(s) 702, 872
Van91I CCANNNNNTGG 3 cut(s) 676, 707, 871
VpaK11BI GGWCC 3 cut(s) 242, 698, 1877
XapI RAATTY 1 cut(s) 233
XceI RCATGY 1 cut(s) 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmiI GTMKAC 1 cut(s) 737
XspI CTAG 2 cut(s) 728, 1181
ZrmI AGTACT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.