Rorug06G0227300

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011526
Physical Location & Seq
Reverse (-)
36493644 .. 36494678
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0227300.1

Sequence Viewer

Length: 897 bp
ATGTTGATCAGAGGTATTGCTTCTGTGGCTGGTAAGTATCTTGAGTTGGATGATAAACTCTTTGCAAATACGGGTAAGGTTAGGGTTCATGTGGCACATGATATTTCGAAACCTTTTTTCCTCAAGAAAACCCTAAAACTGGCACCTGGAGTAGAGGTCGAAATTTCATATTTTTTTGAAAACCTGGTAGGCAAGTGTGATCATTGCAATTTGATTTATCATGAGGGAGACTATTGCCCTTTGCTTGGTAGCCATGCCACAACAGGTATAAGACGCAGCACTGGAGACCAGAGGAAAGATTTGGTTGGTCCTTCTCTAGGGTTTGCGGCTAACACTTTTGTAGATGGAACGTTTCGTTTTCAAGGTGTGCAGACACCTATTTTGCCATCTATCTACAGGTCCTTATTCCAACCCAAGGAAGCTTCTAAAGCACGCAAACCTATCATACTGCGTGACAGAGTCCTAAGTGCCACAGAATTGGAGAAGGAGGATGCAAAACCAGCTGTACCGGTTGAAGCAGAGAACAAGGAGAGTAGGGGTGACAAGCGTCACCTCCCCCCCTCTCCTTCAAAGTCTCCCCAAAAACTAAAGATTCTGCTACCAGAATTATTCAAACCGTTGCTGCCAGATATCCAGGCAAAGAAACGAAAACTGCCTATGTTTACAAGTAAGTTCTCTGCCAGATCTTTGGGTATGGTTGAAGCTCCTGGAAATATTTTAGTGCCTAAGGATGTGATGCAAACAAACTCTGGCACAAAGAAGAAAAGAGGAAGGCCTCTGGGAGCAAAGAATAAAAATCCTCCAAAGTCAAAGAAGGTTGCGGATGCGCAAGAGACTTGTTTTGCTTATCTCTCCAAACCTCCTAGCCCAACTGTTAAGGGCAAGGAGAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

298

Amino Acids

32.95

Weight (kDa)

9.87

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 828
AccB1I GGYRCC 1 cut(s) 142
AciI CCGC 2 cut(s) 326, 821
AclI AACGTT 1 cut(s) 350
AcsI RAATTY 2 cut(s) 162, 891
AfaI GTAC 1 cut(s) 507
AfiI CCNNNNNNNGG 4 cut(s) 139, 245, 317, 415
AgeI ACCGGT 1 cut(s) 508
AgsI TTSAA 6 cut(s) 179, 362, 515, 570, 613, 701
AjnI CCWGG 4 cut(s) 145, 183, 633, 706
AluBI AGCT 3 cut(s) 422, 503, 704
AluI AGCT 3 cut(s) 422, 503, 704
Alw26I GTCTC 4 cut(s) 222, 279, 579, 827
AoxI GGCC 1 cut(s) 773
ApeKI GCWGC 2 cut(s) 276, 622
ApoI RAATTY 2 cut(s) 162, 891
ArsI GACNNNNNNTTYG 2 cut(s) 364, 396
AsiGI ACCGGT 1 cut(s) 508
AspLEI GCGC 1 cut(s) 829
AspS9I GGNCC 2 cut(s) 308, 399
AsuHPI GGTGA 2 cut(s) 542, 551
AsuII TTCGAA 1 cut(s) 107
AvaII GGWCC 2 cut(s) 308, 399
AxyI CCTNAGG 1 cut(s) 726
BanI GGYRCC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 288, 609
BccI CCATC 2 cut(s) 338, 394
BciT130I CCWGG 4 cut(s) 147, 185, 635, 708
BclI TGATCA 2 cut(s) 6, 199
BcoDI GTCTC 4 cut(s) 222, 279, 579, 827
BfaI CTAG 2 cut(s) 317, 864
BfmI CTRYAG 1 cut(s) 394
BglII AGATCT 1 cut(s) 683
BisI GCNGC 3 cut(s) 277, 327, 623
BlsI GCNGC 3 cut(s) 278, 328, 624
Bme1390I CCNGG 4 cut(s) 147, 185, 635, 708
Bme18I GGWCC 2 cut(s) 308, 399
BmgT120I GGNCC 2 cut(s) 308, 399
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 4 cut(s) 147, 185, 635, 708
BmsI GCATC 3 cut(s) 481, 726, 814
BoxI GACNNNNGTC 1 cut(s) 546
BpmI CTGGAG 2 cut(s) 168, 303
Bpu14I TTCGAA 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 62, 107
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 245, 317, 415
Bse118I RCCGGY 1 cut(s) 508
Bse1I ACTGG 2 cut(s) 144, 286
Bse21I CCTNAGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 202
BseBI CCWGG 4 cut(s) 147, 185, 635, 708
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 4 cut(s) 55, 496, 736, 829
BseLI CCNNNNNNNGG 4 cut(s) 139, 245, 317, 415
BseMI GCAATG 1 cut(s) 202
BseNI ACTGG 2 cut(s) 144, 286
BseXI GCAGC 2 cut(s) 288, 609
BsgI GTGCAG 1 cut(s) 389
BshFI GGCC 1 cut(s) 775
BshNI GGYRCC 1 cut(s) 142
BshTI ACCGGT 1 cut(s) 508
BsiSI CCGG 1 cut(s) 509
BslI CCNNNNNNNGG 4 cut(s) 139, 245, 317, 415
BsmAI GTCTC 4 cut(s) 222, 279, 579, 827
BsnI GGCC 1 cut(s) 775
Bso31I GGTCTC 1 cut(s) 279
Bsp119I TTCGAA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 6, 199, 683
BspACI CCGC 2 cut(s) 326, 821
BspANI GGCC 1 cut(s) 775
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 1 cut(s) 144
BspT104I TTCGAA 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 508
BsrI ACTGG 2 cut(s) 144, 286
BssAI RCCGGY 1 cut(s) 508
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 3 cut(s) 6, 199, 683
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 4 cut(s) 147, 185, 635, 708
Bst4CI ACNGT 2 cut(s) 618, 874
BstBI TTCGAA 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 464, 726
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 4 cut(s) 55, 496, 736, 829
BstHHI GCGC 1 cut(s) 829
BstKTI GATC 3 cut(s) 9, 202, 686
BstMAI GTCTC 4 cut(s) 222, 279, 579, 827
BstMBI GATC 3 cut(s) 6, 199, 683
BstMWI GCNNNNNNNGC 3 cut(s) 26, 428, 500
BstNI CCWGG 4 cut(s) 147, 185, 635, 708
BstPAI GACNNNNGTC 1 cut(s) 546
BstSCI CCNGG 4 cut(s) 145, 183, 633, 706
BstSFI CTRYAG 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 288, 609
BstX2I RGATCY 1 cut(s) 683
BstXI CCANNNNNNTGG 2 cut(s) 478, 688
BstYI RGATCY 1 cut(s) 683
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 1 cut(s) 775
BtsCI GGATG 4 cut(s) 55, 496, 736, 829
BtsIMutI CAGTG 1 cut(s) 279
Cac8I GCNNGC 1 cut(s) 433
CciI TCATGA 1 cut(s) 220
CfoI GCGC 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 508
Cfr13I GGNCC 2 cut(s) 308, 399
CseI GACGC 2 cut(s) 282, 536
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 1 cut(s) 506
CspAI ACCGGT 1 cut(s) 508
CviAII CATG 4 cut(s) 89, 98, 221, 254
CviJI RGCY 8 cut(s) 29, 252, 329, 422, 503, 704, 775, 867
CviKI_1 RGCY 8 cut(s) 29, 252, 329, 422, 503, 704, 775, 867
CviQI GTAC 1 cut(s) 506
DdeI CTNAG 2 cut(s) 464, 726
DpnI GATC 3 cut(s) 8, 201, 685
DpnII GATC 3 cut(s) 6, 199, 683
Eco130I CCWWGG 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 279
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 2 cut(s) 308, 399
Eco81I CCTNAGG 1 cut(s) 726
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 399
EcoRII CCWGG 4 cut(s) 145, 183, 633, 706
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 4 cut(s) 92, 101, 224, 257
FaiI YATR 9 cut(s) 90, 99, 169, 222, 255, 269, 446, 659, 695
FatI CATG 4 cut(s) 88, 97, 220, 253
FbaI TGATCA 2 cut(s) 6, 199
Fnu4HI GCNGC 3 cut(s) 277, 327, 623
FokI GGATG 4 cut(s) 62, 503, 743, 836
Fsp4HI GCNGC 3 cut(s) 277, 327, 623
FspBI CTAG 2 cut(s) 317, 864
FspI TGCGCA 1 cut(s) 828
GlaI GCGC 1 cut(s) 828
GluI GCNGC 3 cut(s) 277, 327, 623
GsuI CTGGAG 2 cut(s) 168, 303
HaeIII GGCC 1 cut(s) 775
HapII CCGG 1 cut(s) 509
HgaI GACGC 2 cut(s) 282, 536
HhaI GCGC 1 cut(s) 829
Hin1II CATG 4 cut(s) 92, 101, 224, 257
Hin6I GCGC 1 cut(s) 827
HinP1I GCGC 1 cut(s) 827
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 2 cut(s) 459, 592
HpaII CCGG 1 cut(s) 509
HphI GGTGA 2 cut(s) 542, 551
Hpy166II GTNNAC 1 cut(s) 663
Hpy188I TCNGA 1 cut(s) 11
Hpy188III TCNNGA 3 cut(s) 41, 124, 221
Hpy8I GTNNAC 1 cut(s) 663
HpyAV CCTTC 5 cut(s) 321, 478, 576, 765, 808
HpyCH4III ACNGT 2 cut(s) 618, 874
HpyCH4IV ACGT 1 cut(s) 350
HpyCH4V TGCA 5 cut(s) 65, 207, 370, 494, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 428, 500
HpyF3I CTNAG 2 cut(s) 464, 726
HpySE526I ACGT 1 cut(s) 350
Hsp92II CATG 4 cut(s) 92, 101, 224, 257
HspAI GCGC 1 cut(s) 827
Ksp22I TGATCA 2 cut(s) 6, 199
Kzo9I GATC 3 cut(s) 6, 199, 683
LmnI GCTCC 2 cut(s) 709, 782
Lsp1109I GCAGC 2 cut(s) 288, 609
LweI GCATC 3 cut(s) 481, 726, 814
MabI ACCWGGT 1 cut(s) 183
MaeI CTAG 2 cut(s) 317, 864
MaeII ACGT 1 cut(s) 350
MaeIII GTNAC 3 cut(s) 452, 539, 548
MalI GATC 3 cut(s) 8, 201, 685
MboI GATC 3 cut(s) 6, 199, 683
MboII GAAGA 1 cut(s) 772
MflI RGATCY 1 cut(s) 683
MluCI AATT 5 cut(s) 162, 208, 476, 605, 891
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 2 cut(s) 27, 433
MseI TTAA 2 cut(s) 876, 895
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 4 cut(s) 147, 185, 635, 708
MvaI CCWGG 4 cut(s) 147, 185, 635, 708
MwoI GCNNNNNNNGC 3 cut(s) 26, 428, 500
NdeII GATC 3 cut(s) 6, 199, 683
NlaIII CATG 4 cut(s) 92, 101, 224, 257
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 3 cut(s) 452, 539, 548
NsbI TGCGCA 1 cut(s) 828
NspV TTCGAA 1 cut(s) 107
PagI TCATGA 1 cut(s) 220
PceI AGGCCT 1 cut(s) 775
PfeI GAWTC 1 cut(s) 592
PflFI GACNNNGTC 1 cut(s) 458
PfoI TCCNGGA 1 cut(s) 706
PinAI ACCGGT 1 cut(s) 508
PkrI GCNGC 3 cut(s) 278, 328, 624
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 399
PshAI GACNNNNGTC 1 cut(s) 546
Psp1406I AACGTT 1 cut(s) 350
Psp5II RGGWCCY 1 cut(s) 399
Psp6I CCWGG 4 cut(s) 145, 183, 633, 706
PspGI CCWGG 4 cut(s) 145, 183, 633, 706
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 2 cut(s) 308, 399
PspPPI RGGWCCY 1 cut(s) 399
PsuI RGATCY 1 cut(s) 683
PsyI GACNNNGTC 1 cut(s) 458
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 1 cut(s) 507
RsaNI GTAC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 876, 895
SatI GCNGC 3 cut(s) 277, 327, 623
Sau3AI GATC 3 cut(s) 6, 199, 683
Sau96I GGNCC 2 cut(s) 308, 399
SchI GAGTC 1 cut(s) 468
ScrFI CCNGG 4 cut(s) 147, 185, 635, 708
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 3 cut(s) 481, 726, 814
SfcI CTRYAG 1 cut(s) 394
SfuI TTCGAA 1 cut(s) 107
SinI GGWCC 2 cut(s) 308, 399
SmlI CTYRAG 2 cut(s) 41, 122
SmoI CTYRAG 2 cut(s) 41, 122
Sse9I AATT 5 cut(s) 162, 208, 476, 605, 891
SseBI AGGCCT 1 cut(s) 775
SsiI CCGC 2 cut(s) 326, 821
SspI AATATT 1 cut(s) 715
SspMI CTAG 2 cut(s) 317, 864
StuI AGGCCT 1 cut(s) 775
StyD4I CCNGG 4 cut(s) 145, 183, 633, 706
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 618, 874
TaiI ACGT 1 cut(s) 353
TaqI TCGA 2 cut(s) 107, 159
TasI AATT 5 cut(s) 162, 208, 476, 605, 891
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 1 cut(s) 592
Tru1I TTAA 2 cut(s) 876, 895
Tru9I TTAA 2 cut(s) 876, 895
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 3 cut(s) 452, 539, 548
TseI GCWGC 2 cut(s) 276, 622
Tsp45I GTSAC 3 cut(s) 452, 539, 548
TspDTI ATGAA 2 cut(s) 77, 156
TspRI CASTG 1 cut(s) 286
Tth111I GACNNNGTC 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 308, 399
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 2 cut(s) 162, 891
XspI CTAG 2 cut(s) 317, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.