Rh3DG321700

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011526
Physical Location & Seq
Reverse (-)
34929056 .. 34929412
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG321700.1

Sequence Viewer

Length: 357 bp
ATGATTCCATCCAAAGTCCAGATCAGTGGCTTGAGTTTCAAGAATATTAGGGGCTCATCTTCATCTCCGGTTGATGTCAAGCTCGACTGTAGTCACAGTGCACTGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCATGTACAGTGGAGACAAAAAAGACAAAATTACATCCGAGTGTAATAATGTCAAGCCGAGAATTACTGGCGTGACTGAGGCTTTTGGTTGTACCAATCTAGGTGACATACCGCCGGTGGTAGACACACCTCCCCTTTCGCCGGTGGTGGACACACCTCCTCTTCCTCCTCCCCCTACGGTAGCATCAGATGATTCATCTCCACCGATAGTAATAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

118

Amino Acids

12.44

Weight (kDa)

4.64

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 4 - 64 1.9e-10 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AciI CCGC 1 cut(s) 248
AfaI GTAC 2 cut(s) 143, 229
AfiI CCNNNNNNNGG 2 cut(s) 115, 277
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 2 cut(s) 82, 121
AluI AGCT 2 cut(s) 82, 121
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 1 cut(s) 144
Alw44I GTGCAC 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 103
BanII GRGCYC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 103
BccI CCATC 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 88
BmsI GCATC 1 cut(s) 329
BoxI GACNNNNGTC 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 52
BsaBI GATNNNNATC 1 cut(s) 132
BsaWI WCCGGW 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 277
Bse118I RCCGGY 2 cut(s) 250, 277
Bse1I ACTGG 1 cut(s) 208
Bse8I GATNNNNATC 1 cut(s) 132
BseGI GGATG 2 cut(s) 8, 170
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 2 cut(s) 115, 277
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 2 cut(s) 285, 294
BseSI GKGCMC 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 103
BsiSI CCGG 4 cut(s) 68, 109, 251, 278
BslI CCNNNNNNNGG 2 cut(s) 115, 277
BsmAI GTCTC 1 cut(s) 144
Bsp1286I GDGCHC 2 cut(s) 56, 103
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 21, 133
BspACI CCGC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 205
BsrFI RCCGGY 2 cut(s) 250, 277
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 2 cut(s) 250, 277
BssMI GATC 2 cut(s) 21, 133
Bst4CI ACNGT 5 cut(s) 89, 98, 105, 146, 316
Bst6I CTCTTC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 8, 170
BstKTI GATC 2 cut(s) 24, 136
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 2 cut(s) 21, 133
BstPAI GACNNNNGTC 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 103
BstXI CCANNNNNNTGG 1 cut(s) 26
BtsCI GGATG 2 cut(s) 8, 170
BtsIMutI CAGTG 4 cut(s) 31, 101, 103, 151
Cfr10I RCCGGY 2 cut(s) 250, 277
Csp6I GTAC 2 cut(s) 142, 228
CviAII CATG 1 cut(s) 139
CviJI RGCY 6 cut(s) 30, 54, 82, 121, 193, 218
CviKI_1 RGCY 6 cut(s) 30, 54, 82, 121, 193, 218
CviQI GTAC 2 cut(s) 142, 228
DdeI CTNAG 1 cut(s) 213
DpnI GATC 2 cut(s) 23, 135
DpnII GATC 2 cut(s) 21, 133
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco24I GRGCYC 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 56
FaeI CATG 1 cut(s) 142
FaiI YATR 2 cut(s) 140, 245
FatI CATG 1 cut(s) 138
FblI GTMKAC 1 cut(s) 258
FokI GGATG 1 cut(s) 157
FriOI GRGCYC 1 cut(s) 56
FspBI CTAG 1 cut(s) 236
HapII CCGG 4 cut(s) 68, 109, 251, 278
Hin1II CATG 1 cut(s) 142
HinfI GANTC 2 cut(s) 4, 329
HpaII CCGG 4 cut(s) 68, 109, 251, 278
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 3 cut(s) 101, 259, 286
Hpy188I TCNGA 3 cut(s) 127, 175, 325
Hpy188III TCNNGA 2 cut(s) 19, 40
Hpy8I GTNNAC 3 cut(s) 101, 259, 286
HpyCH4III ACNGT 5 cut(s) 89, 98, 105, 146, 316
HpyCH4V TGCA 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 213
Hsp92II CATG 1 cut(s) 142
Kzo9I GATC 2 cut(s) 21, 133
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 6 cut(s) 32, 81, 122, 189, 264, 291
LweI GCATC 1 cut(s) 329
MaeI CTAG 1 cut(s) 236
MaeIII GTNAC 3 cut(s) 92, 208, 239
MalI GATC 2 cut(s) 23, 135
MboI GATC 2 cut(s) 21, 133
MboII GAAGA 2 cut(s) 51, 290
MhlI GDGCHC 2 cut(s) 56, 103
MluCI AATT 2 cut(s) 165, 198
MnlI CCTC 6 cut(s) 208, 276, 303, 306, 312, 315
MslI CAYNNNNRTG 1 cut(s) 175
MspI CCGG 4 cut(s) 68, 109, 251, 278
NdeII GATC 2 cut(s) 21, 133
NlaIII CATG 1 cut(s) 142
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 3 cut(s) 92, 208, 239
PfeI GAWTC 2 cut(s) 4, 329
PshAI GACNNNNGTC 1 cut(s) 90
RsaI GTAC 2 cut(s) 143, 229
RsaNI GTAC 2 cut(s) 142, 228
RseI CAYNNNNRTG 1 cut(s) 175
Sau3AI GATC 2 cut(s) 21, 133
SduI GDGCHC 2 cut(s) 56, 103
SetI ASST 5 cut(s) 84, 123, 241, 268, 295
SfaNI GCATC 1 cut(s) 329
SfcI CTRYAG 1 cut(s) 88
SgrAI CRCCGGYG 2 cut(s) 250, 277
SmiMI CAYNNNNRTG 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 2 cut(s) 165, 198
SsiI CCGC 1 cut(s) 248
SspI AATATT 1 cut(s) 46
SspMI CTAG 1 cut(s) 236
TaaI ACNGT 5 cut(s) 89, 98, 105, 146, 316
TaqI TCGA 1 cut(s) 84
TasI AATT 2 cut(s) 165, 198
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 2 cut(s) 4, 329
TscAI CASTG 4 cut(s) 31, 103, 108, 151
TseFI GTSAC 3 cut(s) 92, 208, 239
Tsp45I GTSAC 3 cut(s) 92, 208, 239
TspDTI ATGAA 2 cut(s) 51, 321
TspRI CASTG 4 cut(s) 31, 103, 108, 151
VneI GTGCAC 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.