Rorug06G0468000

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011526
Physical Location & Seq
Reverse (-)
59227738 .. 59230163
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0468000.1

Sequence Viewer

Length: 1254 bp
ATGGCCATGGAGAGAGCAAGATCATTCCTTCAGTCTCCCACTTATGGAAACCTCGTCACTGTTCTTAGCATTGATGGAGGTGGAATAAGAGGGATTATCCCCGGAACTATGCTCAGCTTCTTAGAGTCCGAGCTTCAGAAGCTGGATGGGGAAGATGCAAGACTAGCAGATTATTTTGATGTAATTGCAGGGACCAGCACAGGTGGTCTTGTCACTGCTATGCTTACTGCCCCAGATGAAAATAACCGTCCAATATTTGCTGCTAAAGATATTAACGATTTTTATCTTGAACACTGTCCTAAAATCTTCCCCCAAGACAGCACCTCAATAATTGGTGAAGCTGTAGAAAAGTTGAAAGTTGTGAAAGGGCCAAAATATGATGGGGAGTATTTACATAAGATAGTCAGGGAAAAGTTGGGAGACATACGATTGAACGAGACATTGACTAACGTTGTAATCCCAACTTTTGATATAAAGAGGATTCAGCCAGTCATCTTCTCCAGCTATGAGGTTAAGAAGAATGATAGCTTAGATGCTTTACTCTCCGACATATGCATTGGAACTTCAGCAGCCCCAACTTATCTCCCAGCTCATCATTTTGAAACCCAAAATTCCAGAACTGGAGAAGCAAGAGAATTCAATCTGATTGACGGTGCAGTCGCTGCTAATAATCCGGCTTTGGTTGCTATAAACGAAGTAACAAAAGAAATCCACCAAGGAAACACCGACTTCTTTCCTATGAGGCCAACTGAGTATGGGCGATTCCTCGTTATATCACTGGGTACTGGTTCAGGGAAATGCGAGGAGAAGTATGCCGCCGCAAATGCTGCTAAGTGGGGCGTTTTGGGGTGGCTAACCGCAGGTGGTTCATGTCCATTGGTTGACGTATTTACTCAAGCAAGTAGTGACATGGTCGACTTCCACCTAGCCACCGTTTTCCAAGCTCTTCATTCTGAGAAAAATTACCTTCGGATTCAAGACGATTCACTGGAGGGAACAGTGTCTTCAGTGGACATTGCTACGCATGAGAACTTGAACAACCTTGCAAAAGTTGGAGAGGAGTTGTTAAAGACACCGGTTTCAAGGGTGAATATGGAAACTGGTATATATGAGCCTGCCCATCAGTCCACAAATGAAGAGGCTCTCGTAAGGGTAGCGGGAATTCTGTCTAGAGAGAGGAGAGTTCGTGAAATGAGATCACCACATGGGAAGGCTGCAGGCGCAGTTACTTCTAATACTACTAGCTCCGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

417

Amino Acids

45.45

Weight (kDa)

5.2

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 230 2e-24 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AasI GACNNNNNNGTC 1 cut(s) 656
Acc36I ACCTGC 1 cut(s) 851
AccI GTMKAC 1 cut(s) 915
AciI CCGC 4 cut(s) 816, 819, 858, 1157
AclI AACGTT 1 cut(s) 450
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 610, 635, 1161
AcuI CTGAAG 4 cut(s) 14, 119, 549, 990
AfaI GTAC 1 cut(s) 784
AfiI CCNNNNNNNGG 1 cut(s) 44
AgeI ACCGGT 1 cut(s) 1075
AgsI TTSAA 8 cut(s) 290, 355, 433, 602, 640, 977, 1036, 1083
AluBI AGCT 9 cut(s) 117, 133, 142, 341, 504, 528, 590, 944, 1245
AluI AGCT 9 cut(s) 117, 133, 142, 341, 504, 528, 590, 944, 1245
Alw26I GTCTC 3 cut(s) 39, 414, 431
AlwNI CAGNNNCTG 2 cut(s) 142, 662
AoxI GGCC 3 cut(s) 3, 368, 743
ApeKI GCWGC 5 cut(s) 260, 569, 662, 827, 1214
ApoI RAATTY 3 cut(s) 610, 635, 1161
AsiGI ACCGGT 1 cut(s) 1075
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 2 cut(s) 192, 368
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 3 cut(s) 347, 1099, 1191
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 996
BbvI GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BccI CCATC 4 cut(s) 68, 140, 374, 1128
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 3 cut(s) 39, 414, 431
BfaI CTAG 4 cut(s) 164, 926, 1170, 1242
BfmI CTRYAG 2 cut(s) 342, 1215
BfuAI ACCTGC 1 cut(s) 851
BisI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 7 cut(s) 262, 571, 664, 817, 820, 829, 1216
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 192, 368
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 1 cut(s) 788
BmsI GCATC 2 cut(s) 145, 523
BmuI ACTGGG 1 cut(s) 788
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 3 cut(s) 484, 642, 1010
Bpu1102I GCTNAGC 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 879
BpuMI CCSGG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 6, 100, 715
BsaWI WCCGGW 1 cut(s) 1075
BsaXI ACNNNNNCTCC 2 cut(s) 1171, 1201
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
Bse3DI GCAATG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 6, 100, 715
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 3 cut(s) 127, 741, 945
BseNI ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
BseRI GAGGAG 3 cut(s) 818, 1073, 1192
BseXI GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BseYI CCCAGC 1 cut(s) 586
BsgI GTGCAG 1 cut(s) 675
BshFI GGCC 3 cut(s) 5, 370, 745
BshTI ACCGGT 1 cut(s) 1075
BsiSI CCGG 3 cut(s) 102, 674, 1076
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 3 cut(s) 39, 414, 431
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 3 cut(s) 5, 370, 745
Bsp143I GATC 2 cut(s) 20, 1196
Bsp1720I GCTNAGC 1 cut(s) 113
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 4 cut(s) 816, 819, 858, 1157
BspANI GGCC 3 cut(s) 5, 370, 745
BspCNI CTCAG 3 cut(s) 126, 742, 946
BspLI GGNNCC 1 cut(s) 193
BspMAI CTGCAG 1 cut(s) 1219
BspMI ACCTGC 1 cut(s) 851
BspQI GCTCTTC 1 cut(s) 951
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 6 cut(s) 488, 625, 783, 790, 993, 1105
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 3 cut(s) 6, 100, 715
BssMI GATC 2 cut(s) 20, 1196
BssT1I CCWWGG 2 cut(s) 6, 715
Bst4CI ACNGT 6 cut(s) 61, 248, 296, 653, 934, 1000
Bst6I CTCTTC 2 cut(s) 951, 1131
BstAPI GCANNNNNTGC 2 cut(s) 662, 827
BstC8I GCNNGC 2 cut(s) 1116, 1219
BstDEI CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 151
BstHHI GCGC 1 cut(s) 1223
BstKTI GATC 2 cut(s) 23, 1199
BstMAI GTCTC 3 cut(s) 39, 414, 431
BstMBI GATC 2 cut(s) 20, 1196
BstMWI GCNNNNNNNGC 7 cut(s) 139, 164, 662, 683, 824, 827, 1220
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 2 cut(s) 342, 1215
BstV1I GCAGC 5 cut(s) 247, 581, 649, 814, 1201
BstV2I GAAGAC 1 cut(s) 996
BsuRI GGCC 3 cut(s) 5, 370, 745
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 7 cut(s) 57, 213, 292, 776, 986, 1005, 1014
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 1116, 1219
CaiI CAGNNNCTG 2 cut(s) 142, 662
CfoI GCGC 1 cut(s) 1223
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 2 cut(s) 192, 368
Csp6I GTAC 1 cut(s) 783
CspAI ACCGGT 1 cut(s) 1075
CviAII CATG 5 cut(s) 7, 870, 910, 1025, 1205
CviQI GTAC 1 cut(s) 783
DdeI CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
DpnI GATC 2 cut(s) 22, 1198
DpnII GATC 2 cut(s) 20, 1196
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 951, 1131
EarI CTCTTC 2 cut(s) 951, 1131
Eco130I CCWWGG 2 cut(s) 6, 715
Eco47I GGWCC 1 cut(s) 192
Eco57I CTGAAG 4 cut(s) 14, 119, 549, 990
EcoRI GAATTC 2 cut(s) 635, 1161
EcoT14I CCWWGG 2 cut(s) 6, 715
EcoT22I ATGCAT 1 cut(s) 557
ErhI CCWWGG 2 cut(s) 6, 715
FaeI CATG 5 cut(s) 10, 873, 913, 1028, 1208
FaqI GGGAC 1 cut(s) 205
FatI CATG 5 cut(s) 6, 869, 909, 1024, 1204
FauI CCCGC 1 cut(s) 1150
FauNDI CATATG 1 cut(s) 551
FblI GTMKAC 1 cut(s) 915
Fnu4HI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
FspBI CTAG 4 cut(s) 164, 926, 1170, 1242
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 3 cut(s) 484, 642, 1010
HaeIII GGCC 3 cut(s) 5, 370, 745
HapII CCGG 3 cut(s) 102, 674, 1076
HhaI GCGC 1 cut(s) 1223
Hin1II CATG 5 cut(s) 10, 873, 913, 1028, 1208
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HincII GTYRAC 2 cut(s) 883, 916
HindII GTYRAC 2 cut(s) 883, 916
HinfI GANTC 5 cut(s) 125, 481, 762, 973, 983
HpaII CCGG 3 cut(s) 102, 674, 1076
HphI GGTGA 3 cut(s) 347, 1099, 1191
Hpy166II GTNNAC 4 cut(s) 883, 916, 1012, 1128
Hpy188I TCNGA 7 cut(s) 130, 138, 547, 645, 955, 972, 1249
Hpy188III TCNNGA 5 cut(s) 287, 615, 977, 1170, 1187
Hpy8I GTNNAC 4 cut(s) 883, 916, 1012, 1128
HpyAV CCTTC 3 cut(s) 38, 977, 1204
HpyCH4III ACNGT 6 cut(s) 61, 248, 296, 653, 934, 1000
HpyCH4IV ACGT 2 cut(s) 450, 885
HpyCH4V TGCA 6 cut(s) 158, 188, 555, 656, 1046, 1217
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 164, 662, 683, 824, 827, 1220
HpyF3I CTNAG 7 cut(s) 65, 113, 121, 529, 750, 831, 954
HpySE526I ACGT 2 cut(s) 450, 885
Hsp92II CATG 5 cut(s) 10, 873, 913, 1028, 1208
HspAI GCGC 1 cut(s) 1221
Kzo9I GATC 2 cut(s) 20, 1196
LguI GCTCTTC 1 cut(s) 951
LmnI GCTCC 1 cut(s) 1250
Lsp1109I GCAGC 5 cut(s) 247, 581, 649, 814, 1201
LweI GCATC 2 cut(s) 145, 523
MaeI CTAG 4 cut(s) 164, 926, 1170, 1242
MaeII ACGT 2 cut(s) 450, 885
MaeIII GTNAC 5 cut(s) 55, 211, 697, 905, 1225
MalI GATC 2 cut(s) 22, 1198
MboI GATC 2 cut(s) 20, 1196
MboII GAAGA 7 cut(s) 164, 298, 487, 529, 938, 996, 1148
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 570, 1033
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 557
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 273, 513, 1067
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 102, 674, 1076
MspR9I CCNGG 1 cut(s) 102
MwoI GCNNNNNNNGC 7 cut(s) 139, 164, 662, 683, 824, 827, 1220
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 551
NdeII GATC 2 cut(s) 20, 1196
NlaIII CATG 5 cut(s) 10, 873, 913, 1028, 1208
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 3 cut(s) 55, 211, 905
NsiI ATGCAT 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 851
PciSI GCTCTTC 1 cut(s) 951
PfeI GAWTC 4 cut(s) 481, 762, 973, 983
PflFI GACNNNGTC 1 cut(s) 911
PinAI ACCGGT 1 cut(s) 1075
PkrI GCNGC 7 cut(s) 262, 571, 664, 817, 820, 829, 1216
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Psp1406I AACGTT 1 cut(s) 450
PspFI CCCAGC 1 cut(s) 586
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 2 cut(s) 192, 368
PstI CTGCAG 1 cut(s) 1219
PstNI CAGNNNCTG 2 cut(s) 142, 662
PsyI GACNNNGTC 1 cut(s) 911
RsaI GTAC 1 cut(s) 784
RsaNI GTAC 1 cut(s) 783
RseI CAYNNNNRTG 1 cut(s) 218
SalI GTCGAC 1 cut(s) 914
SapI GCTCTTC 1 cut(s) 951
SaqAI TTAA 3 cut(s) 273, 513, 1067
SatI GCNGC 7 cut(s) 261, 570, 663, 816, 819, 828, 1215
Sau3AI GATC 2 cut(s) 20, 1196
Sau96I GGNCC 2 cut(s) 192, 368
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 102
SfaNI GCATC 2 cut(s) 145, 523
SfcI CTRYAG 2 cut(s) 342, 1215
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 894
SmoI CTYRAG 1 cut(s) 894
Sse9I AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
SsiI CCGC 4 cut(s) 816, 819, 858, 1157
SspI AATATT 1 cut(s) 255
SspMI CTAG 4 cut(s) 164, 926, 1170, 1242
StyD4I CCNGG 1 cut(s) 100
StyI CCWWGG 2 cut(s) 6, 715
TaaI ACNGT 6 cut(s) 61, 248, 296, 653, 934, 1000
TaiI ACGT 2 cut(s) 453, 888
TaqI TCGA 1 cut(s) 915
TasI AATT 6 cut(s) 183, 330, 610, 635, 961, 1161
TauI GCSGC 2 cut(s) 818, 821
TfiI GAWTC 4 cut(s) 481, 762, 973, 983
Tru1I TTAA 3 cut(s) 273, 513, 1067
Tru9I TTAA 3 cut(s) 273, 513, 1067
TscAI CASTG 7 cut(s) 64, 220, 299, 783, 993, 1005, 1014
TseFI GTSAC 3 cut(s) 55, 211, 905
TseI GCWGC 5 cut(s) 260, 569, 662, 827, 1214
Tsp45I GTSAC 3 cut(s) 55, 211, 905
TspDTI ATGAA 4 cut(s) 252, 858, 938, 1149
TspRI CASTG 7 cut(s) 64, 220, 299, 783, 993, 1005, 1014
Tth111I GACNNNGTC 1 cut(s) 911
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 3 cut(s) 610, 635, 1161
XbaI TCTAGA 1 cut(s) 1169
XmiI GTMKAC 1 cut(s) 915
XspI CTAG 4 cut(s) 164, 926, 1170, 1242
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.