RLG00000012018

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0011526
Physical Location & Seq
Forward (+)
14596490 .. 14601462
4973 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012018

Sequence Viewer

Length: 2067 bp
ATGGGTCTGAAATCGATGAACATTTTGGCAATACTTTTGCTCTCATTTTTAGCATCCACCGCTGTAGCTGATCAATCCATTGTCTTCGATGTGACAAGCTCAAAATATGGTGGAAAGCCCAACTCGGATATTTCAAAGGCATTGGCAATGGCATGGACAGATGCGTGTGCATCAACGTCAACGAGTGCTGTCCTTGTTCCGAGGGGAACATACAAGTTATTACAAGAAGCAAAATTTGAAGGTCCTTGCAAGGCTCCTATTCAGTTTCAGCTCCAAGGAACATTGCAGGCTCCGAAAGACCGAAGCCAACTCGATGACTCTTGGATTTCCTTTAACAACATCGACTTGCTCACCTTGTCCGGCGGTGGAACTACCGACGGACAAGGAGCCCTTTCTTGGAAGGAGAATCATGACTCTACTCCCGTTGATATTAGGTTCCAAACTGTCAACAATTCCACAATTAGCGACATAGCTTCAATCGACAGCGCAAAGTTCCACATTAGTGTTCATGGCTGCAACAACGTTACGTTCCAACGGCTCACCATCACAGCACCCGAAGACAGTCCTAACACAGACGGAATCCACATCTATAGTTCGACCAAGATCACAGTCTCCGACTCCTACATTGCAACCGGTGACGATTGTGTTTCAATTAGCTATGGCTCCAACCAAATCCTAGTGTCCAACATTACTTGCAGACCAGGTCATGGAATAAGCATAGGCAGTCTAGGAAAGTATCCAGACGAACAGCCAGTGGTCGGCGTCACGGTCAAGAACTGCACGCTTACCGGCACAAGCAATGGCGCCAGAATCAAGACATGGCCCAATTCTCCGGCAACAAGCGTTGCTCGGAATATACTCATCCAAGACATGGTCATGGTGAATGTGAAGAACCCTATCCTCATCGACCAGAACTACTGTGACAAAGGCAAGTCGTGTAAGCTAATGATTCCATCCAAAGTCCAGATCAGTAACGTGACGTTCAAGGATATCAGGGGCTCGTCTTCGTCTCCGGTTGCCGTCAAGCTCGACTTGGAAACAAAAAAGACGAAATCACGTCTGTGTAATAATGTCAAGCCGAGAATTACTGGCGTGACTAAGGCTCTTGCTTGTGCCAATCTAGGTGACATACCAATAGATAATTCACCGCCGCCATCGACTTCACCGCCTCTTCCACCTCCGCTACCGGTTTCACATCCTCTTCCACCGCCGCTGCCGGCTTCACCTCCTCTTCCAGTACAACAGTCACTACCGCCGCCTCCGGTAGAAAAACGCCATTCCCCTTCTCCGCCACCATTTTCGCCTCCTCTTTCACCGCCGGCGCCATTTGTTTCACCTTCACCGCCACCATCTTTACCTCCTTTTTTGCCGCCGCCATTTGTTTTACCTTCTCCACCACCATCTTCACCTCCTCTTTCGCCGCCGGAGCCATTTATTTCACCTTCACCGCCACCATCTTTACCTCCTCTTTTGCCGCCGCCATTCGTTTTACCTTCTCCACCACCATCTTCACCTCCTCTTTCGCCGCCGGAGCCATTTTTATCTCCTCTTTTGCCGCCGCCATTCATTTTACCTACTCCACCACCATCTTCACCTCCTCTTTCACCGCCGGAGCCATTTATTTCACCTTCACCGCTACCATCTTTACCTCCTCCTTTGCCGCCGCCATTCGTTTTACCTTCTCCTCCACAATCTTCACCTCCTCTTTCGCCGCCGGCGCCATTTGTTTTACCTCCACCGCCACCATCTTTACCTCTTCTTTTGCCGCCTCCATTAGTTTCACCTTCTCCTCCACCATTTTTACTGCCTCTTTCGCCGCCACCATTCGTATCACCTCCTCTTCCGCCGATGGTGTTCACACCCCCTCCCCCTACGGTAGCATCAGACGATTCGTCTCCGCCGATAATAATGGTATATGGGTATCCATATCCTCTACCTCGTACTGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGCAGTATTTTTGGGCAAAAACCGACAGTCAGAATGATGGTGCTTAGTTCCGTCCCGGCTTTGTGGTTGCTGCACGCCTTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

689

Amino Acids

72.4

Weight (kDa)

7.53

Isoelectric Point (pI)

85.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 56 - 373 6.5e-87 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 803, 1321, 1717
AccB7I CCANNNNNTGG 2 cut(s) 707, 871
AclI AACGTT 1 cut(s) 522
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 4 cut(s) 762, 804, 1322, 1718
AfaI GTAC 2 cut(s) 1239, 1942
AfiI CCNNNNNNNGG 4 cut(s) 396, 707, 758, 871
AgeI ACCGGT 2 cut(s) 632, 1186
AgsI TTSAA 5 cut(s) 135, 239, 477, 651, 985
AhdI GACNNNNNGTC 1 cut(s) 1891
AjiI CACGTC 1 cut(s) 1058
AjnI CCWGG 1 cut(s) 700
AjuI GAANNNNNNNTTGG 2 cut(s) 1126, 1158
AleI CACNNNNGTG 2 cut(s) 501, 1060
AluBI AGCT 7 cut(s) 68, 99, 271, 473, 657, 943, 1027
AluI AGCT 7 cut(s) 68, 99, 271, 473, 657, 943, 1027
Alw26I GTCTC 3 cut(s) 616, 1014, 1899
AoxI GGCC 1 cut(s) 821
ApeKI GCWGC 3 cut(s) 513, 1213, 2050
ApoI RAATTY 1 cut(s) 233
AsiGI ACCGGT 2 cut(s) 632, 1186
AspLEI GCGC 4 cut(s) 488, 806, 1324, 1720
AspS9I GGNCC 3 cut(s) 242, 822, 1961
AsuC2I CCSGG 1 cut(s) 2036
AvaII GGWCC 2 cut(s) 242, 1961
BanI GGYRCC 3 cut(s) 803, 1321, 1717
BanII GRGCYC 2 cut(s) 391, 1001
BbsI GAAGAC 3 cut(s) 76, 564, 996
BbvI GCAGC 3 cut(s) 500, 1200, 2037
BceAI ACGGC 2 cut(s) 551, 1004
BciT130I CCWGG 1 cut(s) 702
BciVI GTATCC 2 cut(s) 747, 1932
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 2036
BcoDI GTCTC 3 cut(s) 616, 1014, 1899
BfaI CTAG 3 cut(s) 677, 728, 1121
BfmI CTRYAG 2 cut(s) 63, 589
BfoI RGCGCY 3 cut(s) 807, 1325, 1721
BfuI GTATCC 2 cut(s) 747, 1932
Bme1390I CCNGG 2 cut(s) 702, 2036
Bme18I GGWCC 2 cut(s) 242, 1961
BmeRI GACNNNNNGTC 1 cut(s) 1891
BmgBI CACGTC 1 cut(s) 1058
BmgT120I GGNCC 3 cut(s) 242, 822, 1961
BmrFI CCNGG 2 cut(s) 702, 2036
BmsI GCATC 4 cut(s) 62, 151, 179, 1889
BpiI GAAGAC 3 cut(s) 76, 564, 996
BpuMI CCSGG 1 cut(s) 2036
Bsa29I ATCGAT 1 cut(s) 14
BsaHI GRCGYC 4 cut(s) 762, 804, 1322, 1718
BsaJI CCNNGG 2 cut(s) 200, 274
BsaWI WCCGGW 4 cut(s) 632, 1012, 1186, 1261
BsaXI ACNNNNNCTCC 2 cut(s) 1849, 1879
Bsc4I CCNNNNNNNGG 4 cut(s) 396, 707, 758, 871
Bse118I RCCGGY 7 cut(s) 632, 788, 1186, 1216, 1318, 1714, 1979
Bse1I ACTGG 3 cut(s) 752, 1093, 1235
Bse3DI GCAATG 4 cut(s) 153, 281, 624, 805
BseBI CCWGG 1 cut(s) 702
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 200, 274
BseGI GGATG 5 cut(s) 53, 861, 953, 1195, 1967
BseLI CCNNNNNNNGG 4 cut(s) 396, 707, 758, 871
BseMI GCAATG 4 cut(s) 153, 281, 624, 805
BseNI ACTGG 3 cut(s) 752, 1093, 1235
BseXI GCAGC 3 cut(s) 500, 1200, 2037
BsgI GTGCAG 2 cut(s) 763, 2036
BshFI GGCC 1 cut(s) 823
BshNI GGYRCC 3 cut(s) 803, 1321, 1717
BshTI ACCGGT 2 cut(s) 632, 1186
BshVI ATCGAT 1 cut(s) 14
BslFI GGGAC 1 cut(s) 2018
BslI CCNNNNNNNGG 4 cut(s) 396, 707, 758, 871
BsmAI GTCTC 3 cut(s) 616, 1014, 1899
BsmBI CGTCTC 2 cut(s) 1014, 1899
BsmFI GGGAC 1 cut(s) 2018
BsnI GGCC 1 cut(s) 823
Bsp1286I GDGCHC 2 cut(s) 391, 1001
Bsp143I GATC 3 cut(s) 70, 603, 966
BspANI GGCC 1 cut(s) 823
BspDI ATCGAT 1 cut(s) 14
BspHI TCATGA 1 cut(s) 409
BspT107I GGYRCC 3 cut(s) 803, 1321, 1717
BsrDI GCAATG 4 cut(s) 153, 281, 624, 805
BsrFI RCCGGY 7 cut(s) 632, 788, 1186, 1216, 1318, 1714, 1979
BsrI ACTGG 3 cut(s) 752, 1093, 1235
BssAI RCCGGY 7 cut(s) 632, 788, 1186, 1216, 1318, 1714, 1979
BssECI CCNNGG 2 cut(s) 200, 274
BssMI GATC 3 cut(s) 70, 603, 966
BssNI GRCGYC 4 cut(s) 762, 804, 1322, 1718
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 8 cut(s) 445, 563, 610, 769, 920, 1245, 1876, 2008
Bst6I CTCTTC 5 cut(s) 1176, 1206, 1236, 1761, 1845
BstACI GRCGYC 4 cut(s) 762, 804, 1322, 1718
BstC8I GCNNGC 7 cut(s) 288, 782, 1218, 1320, 1716, 1981, 2055
BstDEI CTNAG 2 cut(s) 1098, 2024
BstF5I GGATG 5 cut(s) 53, 861, 953, 1195, 1967
BstH2I RGCGCY 3 cut(s) 807, 1325, 1721
BstHHI GCGC 4 cut(s) 488, 806, 1324, 1720
BstKTI GATC 3 cut(s) 73, 606, 969
BstMAI GTCTC 3 cut(s) 616, 1014, 1899
BstMBI GATC 3 cut(s) 70, 603, 966
BstMWI GCNNNNNNNGC 5 cut(s) 59, 1426, 1531, 1717, 1813
BstNI CCWGG 1 cut(s) 702
BstSCI CCNGG 2 cut(s) 700, 2034
BstSFI CTRYAG 2 cut(s) 63, 589
BstV1I GCAGC 3 cut(s) 500, 1200, 2037
BstV2I GAAGAC 3 cut(s) 76, 564, 996
Bsu15I ATCGAT 1 cut(s) 14
BsuI GTATCC 2 cut(s) 747, 1932
BsuRI GGCC 1 cut(s) 823
BsuTUI ATCGAT 1 cut(s) 14
BtrI CACGTC 1 cut(s) 1058
BtsCI GGATG 5 cut(s) 53, 861, 953, 1195, 1967
BtsIMutI CAGTG 1 cut(s) 759
Cac8I GCNNGC 7 cut(s) 288, 782, 1218, 1320, 1716, 1981, 2055
CciI TCATGA 1 cut(s) 409
CfoI GCGC 4 cut(s) 488, 806, 1324, 1720
Cfr10I RCCGGY 7 cut(s) 632, 788, 1186, 1216, 1318, 1714, 1979
Cfr13I GGNCC 3 cut(s) 242, 822, 1961
ClaI ATCGAT 1 cut(s) 14
CseI GACGC 1 cut(s) 751
CsiI ACCWGGT 1 cut(s) 700
Csp6I GTAC 2 cut(s) 1238, 1941
CspAI ACCGGT 2 cut(s) 632, 1186
CviAII CATG 7 cut(s) 153, 410, 509, 707, 819, 871, 877
CviQI GTAC 2 cut(s) 1238, 1941
DdeI CTNAG 2 cut(s) 1098, 2024
DinI GGCGCC 3 cut(s) 805, 1323, 1719
DpnI GATC 3 cut(s) 72, 605, 968
DpnII GATC 3 cut(s) 70, 603, 966
DriI GACNNNNNGTC 1 cut(s) 1891
Eam1104I CTCTTC 5 cut(s) 1176, 1206, 1236, 1761, 1845
Eam1105I GACNNNNNGTC 1 cut(s) 1891
EarI CTCTTC 5 cut(s) 1176, 1206, 1236, 1761, 1845
EciI GGCGGA 4 cut(s) 1278, 1833, 1887, 1963
Eco130I CCWWGG 1 cut(s) 274
Eco24I GRGCYC 2 cut(s) 391, 1001
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 242, 1961
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 700
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 274
EcoT38I GRGCYC 2 cut(s) 391, 1001
EgeI GGCGCC 3 cut(s) 805, 1323, 1719
EheI GGCGCC 3 cut(s) 805, 1323, 1719
ErhI CCWWGG 1 cut(s) 274
Esp3I CGTCTC 2 cut(s) 1014, 1899
FaeI CATG 7 cut(s) 156, 413, 512, 710, 822, 874, 880
FalI AAGNNNNNCTT 4 cut(s) 375, 407, 1016, 1048
FaqI GGGAC 1 cut(s) 2018
FatI CATG 7 cut(s) 152, 409, 508, 706, 818, 870, 876
FbaI TGATCA 1 cut(s) 70
FokI GGATG 5 cut(s) 40, 848, 940, 1182, 1954
FriOI GRGCYC 2 cut(s) 391, 1001
FspBI CTAG 3 cut(s) 677, 728, 1121
GlaI GCGC 4 cut(s) 487, 805, 1323, 1719
HaeII RGCGCY 3 cut(s) 807, 1325, 1721
HaeIII GGCC 1 cut(s) 823
HgaI GACGC 1 cut(s) 751
HhaI GCGC 4 cut(s) 488, 806, 1324, 1720
Hin1I GRCGYC 4 cut(s) 762, 804, 1322, 1718
Hin1II CATG 7 cut(s) 156, 413, 512, 710, 822, 874, 880
Hin6I GCGC 4 cut(s) 486, 804, 1322, 1718
HinP1I GCGC 4 cut(s) 486, 804, 1322, 1718
HincII GTYRAC 2 cut(s) 180, 448
HindII GTYRAC 2 cut(s) 180, 448
HinfI GANTC 8 cut(s) 317, 406, 413, 579, 617, 810, 949, 1889
Hpy166II GTNNAC 3 cut(s) 180, 448, 1857
Hpy188I TCNGA 8 cut(s) 9, 127, 201, 294, 616, 852, 1885, 2012
Hpy188III TCNNGA 5 cut(s) 410, 740, 772, 814, 964
Hpy8I GTNNAC 3 cut(s) 180, 448, 1857
Hpy99I CGWCG 1 cut(s) 380
HpyCH4III ACNGT 8 cut(s) 445, 563, 610, 769, 920, 1245, 1876, 2008
HpyCH4IV ACGT 6 cut(s) 176, 522, 527, 975, 980, 1057
HpyCH4V TGCA 8 cut(s) 170, 249, 286, 516, 629, 696, 780, 2053
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 1426, 1531, 1717, 1813
HpyF3I CTNAG 2 cut(s) 1098, 2024
HpySE526I ACGT 6 cut(s) 176, 522, 527, 975, 980, 1057
Hsp92I GRCGYC 4 cut(s) 762, 804, 1322, 1718
Hsp92II CATG 7 cut(s) 156, 413, 512, 710, 822, 874, 880
HspAI GCGC 4 cut(s) 486, 804, 1322, 1718
KasI GGCGCC 3 cut(s) 803, 1321, 1717
KroI GCCGGC 4 cut(s) 1216, 1318, 1714, 1979
KroNI GCCGGC 4 cut(s) 1218, 1320, 1716, 1981
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 3 cut(s) 70, 603, 966
LmnI GCTCC 8 cut(s) 259, 276, 295, 386, 668, 1426, 1531, 1612
Lsp1109I GCAGC 3 cut(s) 500, 1200, 2037
LweI GCATC 4 cut(s) 62, 151, 179, 1889
MabI ACCWGGT 1 cut(s) 700
MaeI CTAG 3 cut(s) 677, 728, 1121
MaeII ACGT 6 cut(s) 176, 522, 527, 975, 980, 1057
MalI GATC 3 cut(s) 72, 605, 968
MboI GATC 3 cut(s) 70, 603, 966
MhlI GDGCHC 2 cut(s) 391, 1001
MluCI AATT 7 cut(s) 233, 451, 459, 651, 826, 1083, 1141
Mly113I GGCGCC 3 cut(s) 804, 1322, 1718
MlyI GAGTC 3 cut(s) 311, 407, 611
MmeI TCCRAC 4 cut(s) 556, 639, 690, 708
MreI CGCCGGCG 2 cut(s) 1318, 1714
MroNI GCCGGC 4 cut(s) 1216, 1318, 1714, 1979
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 3 cut(s) 501, 875, 1060
MspA1I CMGCKG 2 cut(s) 62, 1213
MspR9I CCNGG 2 cut(s) 702, 2036
MvaI CCWGG 1 cut(s) 702
MwoI GCNNNNNNNGC 5 cut(s) 59, 1426, 1531, 1717, 1813
NaeI GCCGGC 4 cut(s) 1218, 1320, 1716, 1981
NarI GGCGCC 3 cut(s) 804, 1322, 1718
NciI CCSGG 1 cut(s) 2036
NdeII GATC 3 cut(s) 70, 603, 966
NgoMIV GCCGGC 4 cut(s) 1216, 1318, 1714, 1979
NlaIII CATG 7 cut(s) 156, 413, 512, 710, 822, 874, 880
NmeAIII GCCGAG 1 cut(s) 1104
NmuCI GTSAC 8 cut(s) 91, 635, 763, 920, 976, 1093, 1124, 1245
OliI CACNNNNGTG 2 cut(s) 501, 1060
PagI TCATGA 1 cut(s) 409
PcsI WCGNNNNNNNCGW 1 cut(s) 1898
PdiI GCCGGC 4 cut(s) 1218, 1320, 1716, 1981
PfeI GAWTC 5 cut(s) 406, 579, 810, 949, 1889
PflFI GACNNNGTC 2 cut(s) 702, 872
PflMI CCANNNNNTGG 2 cut(s) 707, 871
PinAI ACCGGT 2 cut(s) 632, 1186
PleI GAGTC 3 cut(s) 311, 407, 611
PluTI GGCGCC 3 cut(s) 807, 1325, 1721
PpsI GAGTC 3 cut(s) 311, 407, 611
PpuMI RGGWCCY 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 522
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PspPI GGNCC 3 cut(s) 242, 822, 1961
PspPPI RGGWCCY 1 cut(s) 242
PsyI GACNNNGTC 2 cut(s) 702, 872
RsaI GTAC 2 cut(s) 1239, 1942
RsaNI GTAC 2 cut(s) 1238, 1941
RseI CAYNNNNRTG 3 cut(s) 501, 875, 1060
SaqAI TTAA 1 cut(s) 333
Sau3AI GATC 3 cut(s) 70, 603, 966
Sau96I GGNCC 3 cut(s) 242, 822, 1961
SchI GAGTC 3 cut(s) 311, 407, 611
ScrFI CCNGG 2 cut(s) 702, 2036
SduI GDGCHC 2 cut(s) 391, 1001
SexAI ACCWGGT 1 cut(s) 700
SfaNI GCATC 4 cut(s) 62, 151, 179, 1889
SfcI CTRYAG 2 cut(s) 63, 589
SfoI GGCGCC 3 cut(s) 805, 1323, 1719
SgrAI CRCCGGYG 2 cut(s) 1318, 1714
SinI GGWCC 2 cut(s) 242, 1961
SmiMI CAYNNNNRTG 3 cut(s) 501, 875, 1060
Sse9I AATT 7 cut(s) 233, 451, 459, 651, 826, 1083, 1141
SspDI GGCGCC 3 cut(s) 803, 1321, 1717
SspMI CTAG 3 cut(s) 677, 728, 1121
StyD4I CCNGG 2 cut(s) 700, 2034
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 8 cut(s) 445, 563, 610, 769, 920, 1245, 1876, 2008
TaiI ACGT 6 cut(s) 179, 525, 530, 978, 983, 1060
TaqI TCGA 9 cut(s) 14, 87, 312, 342, 480, 596, 906, 1029, 1157
TaqII GACCGA 1 cut(s) 315
TasI AATT 7 cut(s) 233, 451, 459, 651, 826, 1083, 1141
TatI WGTACW 1 cut(s) 1237
TfiI GAWTC 5 cut(s) 406, 579, 810, 949, 1889
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 1 cut(s) 759
TseFI GTSAC 8 cut(s) 91, 635, 763, 920, 976, 1093, 1124, 1245
TseI GCWGC 3 cut(s) 513, 1213, 2050
Tsp45I GTSAC 8 cut(s) 91, 635, 763, 920, 976, 1093, 1124, 1245
TspDTI ATGAA 3 cut(s) 32, 497, 1555
TspGWI ACGGA 3 cut(s) 393, 591, 2020
TspRI CASTG 1 cut(s) 759
Tth111I GACNNNGTC 2 cut(s) 702, 872
Van91I CCANNNNNTGG 2 cut(s) 707, 871
VpaK11BI GGWCC 2 cut(s) 242, 1961
XapI RAATTY 1 cut(s) 233
XspI CTAG 3 cut(s) 677, 728, 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.