Rroxscaffold_3G00269040

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011526
Physical Location & Seq
Reverse (-)
61991417 .. 61992591
1175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269040.1

Sequence Viewer

Length: 1128 bp
ATGTCGACGAGTACAGTCCTTGTTCCAAGGGGAACATACGAGTTATTACAACAAGCAACATTTGAAGGTCCTTGTAAGGCTCCTATTCAGTTTCAGCTCCAAGGAACATTGCAGGATCCAAAAGACCGGAGCCAACTCGATGACTCTTGGGTTCAGTTTAAGAACTTGGACATGCTCACCTTGTCCGGCGGTGGAACTTTCGACGGACAAGGAGCGCATTCTTGGAAGGAGCACCATGACTCTACTCCAGTCAATATTAAGTTCAAAACCGTCAACAATTCCATAATTAGCGACATAACTTTTATGGACAGTGCAAAGTTCCACATTGGTGTTCACGACTGCAACAAGGTTGCATTCCAACGGCTCACCATCACAGCACCCGAAGATAGTCCTAACACCGACGGAATCCACATCTCCAGTTCGACCAAGATCACAGTCTCCGACTCCTACATTGCAACTGGTGACGATTGTGTTTCAATTGGCGATGGCTCCAACCAAATCCAGTGTCCAACATTGCTTGCGGACCAGCCGGTGGTCGGCATCACGGTCAAGAACTGCACGCTCACCGGCACAACCAACGGCGCCAGAATCAAGACATCGCCCAATTCTCCGGCAACAAGCGTTGCTTGGGATATACTCTTCCAGGACATGATCATGGTGAATGTGAAGAACCCTATCCTCATTAACCAGAACTACTGCGACAAAGGCAAGTCGTGTAAGCTAATGATTCCATCCAAAGTACAGATCAGTGATGTGAGGTTCAAGAATATCAGGGGCTTGTCTTCATCTCCGGTAGCCGTCAAGCTGGACTGTAGTCACAGCGCGCCGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCACGTACAGTGGTGACGAAAAAGACAAAATCACGTCCGAGTGTAATAATGTCAAGCCGAGACTTACTCGCGTGACTAAGGCTCTTGCTTGTGCCAATCTAGGTGACATACCAATAGATAACTCACCGCCTCGTACTGATGATGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGCTATATTTTGGGGCAAAAACAGACGGTCCGGGTGATGGTGCTAATTAGTTCCACCATGGCTTTGTGGTTGCTGCACGGCTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

375

Amino Acids

40.59

Weight (kDa)

5.9

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 3 - 305 1.7e-71 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 581
AccB7I CCANNNNNTGG 1 cut(s) 532
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 824, 930
AciI CCGC 5 cut(s) 189, 521, 986, 1032, 1035
AclWI GGATC 2 cut(s) 110, 123
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 4 cut(s) 13, 741, 866, 994
AfiI CCNNNNNNNGG 4 cut(s) 532, 536, 838, 1075
AgsI TTSAA 4 cut(s) 65, 265, 477, 763
AjiI CACGTC 1 cut(s) 894
AjnI CCWGG 1 cut(s) 642
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 4 cut(s) 97, 721, 805, 844
AluI AGCT 4 cut(s) 97, 721, 805, 844
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 2 cut(s) 442, 913
AlwI GGATC 2 cut(s) 110, 123
ApeKI GCWGC 1 cut(s) 1111
ArsI GACNNNNNNTTYG 2 cut(s) 490, 522
AspLEI GCGC 4 cut(s) 217, 584, 824, 826
AspS9I GGNCC 4 cut(s) 68, 523, 1019, 1066
AsuC2I CCSGG 1 cut(s) 1070
AsuHPI GGTGA 9 cut(s) 169, 358, 473, 556, 670, 884, 974, 975, 1084
AvaII GGWCC 4 cut(s) 68, 523, 1019, 1066
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 774
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 1 cut(s) 1098
BccI CCATC 5 cut(s) 377, 479, 739, 1001, 1069
BceAI ACGGC 4 cut(s) 377, 595, 782, 811
BciT130I CCWGG 1 cut(s) 644
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 1070
BcoDI GTCTC 2 cut(s) 442, 913
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 1 cut(s) 811
BfoI RGCGCY 1 cut(s) 585
BisI GCNGC 2 cut(s) 1035, 1112
BlsI GCNGC 2 cut(s) 1036, 1113
Bme1390I CCNGG 2 cut(s) 644, 1070
Bme18I GGWCC 4 cut(s) 68, 523, 1019, 1066
BmgBI CACGTC 1 cut(s) 894
BmgT120I GGNCC 4 cut(s) 68, 523, 1019, 1066
BmiI GGNNCC 5 cut(s) 81, 117, 131, 490, 583
BmrFI CCNGG 2 cut(s) 644, 1070
BmsI GCATC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 774
BplI GAGNNNNNCTC 2 cut(s) 910, 942
BpmI CTGGAG 2 cut(s) 231, 400
BpuMI CCSGG 1 cut(s) 1070
BsaAI YACGTR 1 cut(s) 864
BsaBI GATNNNNATC 1 cut(s) 855
BsaHI GRCGYC 1 cut(s) 582
BsaJI CCNNGG 3 cut(s) 26, 100, 1095
BsaWI WCCGGW 2 cut(s) 126, 790
Bsc4I CCNNNNNNNGG 4 cut(s) 532, 536, 838, 1075
Bse118I RCCGGY 3 cut(s) 529, 566, 1037
Bse1I ACTGG 4 cut(s) 248, 417, 463, 502
Bse3DI GCAATG 3 cut(s) 107, 450, 512
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 3 cut(s) 26, 100, 1095
BseGI GGATG 2 cut(s) 731, 1025
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 4 cut(s) 532, 536, 838, 1075
BseMI GCAATG 3 cut(s) 107, 450, 512
BseNI ACTGG 4 cut(s) 248, 417, 463, 502
BsePI GCGCGC 1 cut(s) 822
BseXI GCAGC 1 cut(s) 1098
BsgI GTGCAG 2 cut(s) 541, 1097
Bsh1236I CGCG 2 cut(s) 824, 930
BshNI GGYRCC 1 cut(s) 581
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 9 cut(s) 127, 186, 530, 567, 611, 791, 832, 1038, 1069
BslI CCNNNNNNNGG 4 cut(s) 532, 536, 838, 1075
BsmAI GTCTC 2 cut(s) 442, 913
BsmI GAATGC 2 cut(s) 217, 353
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 5 cut(s) 115, 429, 651, 744, 856
Bsp19I CCATGG 1 cut(s) 1095
BspACI CCGC 5 cut(s) 189, 521, 986, 1032, 1035
BspFNI CGCG 2 cut(s) 824, 930
BspLI GGNNCC 5 cut(s) 81, 117, 131, 490, 583
BspPI GGATC 2 cut(s) 110, 123
BspT107I GGYRCC 1 cut(s) 581
BsrDI GCAATG 3 cut(s) 107, 450, 512
BsrFI RCCGGY 3 cut(s) 529, 566, 1037
BsrI ACTGG 4 cut(s) 248, 417, 463, 502
BssAI RCCGGY 3 cut(s) 529, 566, 1037
BssECI CCNNGG 3 cut(s) 26, 100, 1095
BssHII GCGCGC 1 cut(s) 822
BssMI GATC 5 cut(s) 115, 429, 651, 744, 856
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 3 cut(s) 26, 100, 1095
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 8 cut(s) 16, 271, 311, 436, 547, 812, 869, 1066
Bst6I CTCTTC 1 cut(s) 644
BstACI GRCGYC 1 cut(s) 582
BstBAI YACGTR 1 cut(s) 864
BstC8I GCNNGC 4 cut(s) 519, 560, 824, 1039
BstDEI CTNAG 1 cut(s) 936
BstDSI CCRYGG 1 cut(s) 1095
BstF5I GGATG 2 cut(s) 731, 1025
BstFNI CGCG 2 cut(s) 824, 930
BstH2I RGCGCY 1 cut(s) 585
BstHHI GCGC 4 cut(s) 217, 584, 824, 826
BstKTI GATC 5 cut(s) 118, 432, 654, 747, 859
BstMAI GTCTC 2 cut(s) 442, 913
BstMBI GATC 5 cut(s) 115, 429, 651, 744, 856
BstMWI GCNNNNNNNGC 2 cut(s) 705, 828
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 175
BstPAI GACNNNNGTC 1 cut(s) 813
BstSCI CCNGG 2 cut(s) 642, 1068
BstSFI CTRYAG 1 cut(s) 811
BstUI CGCG 2 cut(s) 824, 930
BstV1I GCAGC 1 cut(s) 1098
BstV2I GAAGAC 1 cut(s) 774
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 1095
BtgZI GCGATG 2 cut(s) 498, 582
BtrI CACGTC 1 cut(s) 894
BtsCI GGATG 2 cut(s) 731, 1025
BtsIMutI CAGTG 4 cut(s) 316, 509, 754, 874
Cac8I GCNNGC 4 cut(s) 519, 560, 824, 1039
CfoI GCGC 4 cut(s) 217, 584, 824, 826
Cfr10I RCCGGY 3 cut(s) 529, 566, 1037
Cfr13I GGNCC 4 cut(s) 68, 523, 1019, 1066
CpoI CGGWCCG 1 cut(s) 1066
Csp6I GTAC 4 cut(s) 12, 740, 865, 993
CspI CGGWCCG 1 cut(s) 1066
CviAII CATG 5 cut(s) 172, 236, 649, 655, 1096
CviQI GTAC 4 cut(s) 12, 740, 865, 993
DdeI CTNAG 1 cut(s) 936
DinI GGCGCC 1 cut(s) 583
DpnI GATC 5 cut(s) 117, 431, 653, 746, 858
DpnII GATC 5 cut(s) 115, 429, 651, 744, 856
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
EciI GGCGGA 1 cut(s) 1021
Eco130I CCWWGG 3 cut(s) 26, 100, 1095
Eco47I GGWCC 4 cut(s) 68, 523, 1019, 1066
EcoO109I RGGNCCY 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 642
EcoT14I CCWWGG 3 cut(s) 26, 100, 1095
EgeI GGCGCC 1 cut(s) 583
EheI GGCGCC 1 cut(s) 583
ErhI CCWWGG 3 cut(s) 26, 100, 1095
FaeI CATG 5 cut(s) 175, 239, 652, 658, 1099
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FatI CATG 5 cut(s) 171, 235, 648, 654, 1095
FbaI TGATCA 1 cut(s) 651
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 1035, 1112
FokI GGATG 2 cut(s) 718, 1012
Fsp4HI GCNGC 2 cut(s) 1035, 1112
FspBI CTAG 1 cut(s) 959
GlaI GCGC 4 cut(s) 216, 583, 823, 825
GluI GCNGC 2 cut(s) 1035, 1112
GsuI CTGGAG 2 cut(s) 231, 400
HaeII RGCGCY 1 cut(s) 585
HapII CCGG 9 cut(s) 127, 186, 530, 567, 611, 791, 832, 1038, 1069
HhaI GCGC 4 cut(s) 217, 584, 824, 826
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 5 cut(s) 175, 239, 652, 658, 1099
Hin6I GCGC 4 cut(s) 215, 582, 822, 824
HinP1I GCGC 4 cut(s) 215, 582, 822, 824
HincII GTYRAC 2 cut(s) 6, 274
HindII GTYRAC 2 cut(s) 6, 274
HinfI GANTC 6 cut(s) 143, 239, 405, 443, 588, 727
HpaII CCGG 9 cut(s) 127, 186, 530, 567, 611, 791, 832, 1038, 1069
HphI GGTGA 9 cut(s) 169, 358, 473, 556, 670, 884, 974, 975, 1084
Hpy166II GTNNAC 3 cut(s) 6, 274, 334
Hpy188I TCNGA 3 cut(s) 442, 850, 898
Hpy188III TCNNGA 4 cut(s) 335, 550, 592, 763
Hpy8I GTNNAC 3 cut(s) 6, 274, 334
Hpy99I CGWCG 3 cut(s) 10, 206, 404
HpyAV CCTTC 2 cut(s) 59, 220
HpyCH4III ACNGT 8 cut(s) 16, 271, 311, 436, 547, 812, 869, 1066
HpyCH4IV ACGT 2 cut(s) 863, 893
HpyCH4V TGCA 7 cut(s) 112, 314, 342, 353, 455, 558, 1114
HpyF10VI GCNNNNNNNGC 2 cut(s) 705, 828
HpyF3I CTNAG 1 cut(s) 936
HpySE526I ACGT 2 cut(s) 863, 893
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 5 cut(s) 175, 239, 652, 658, 1099
HspAI GCGC 4 cut(s) 215, 582, 822, 824
KasI GGCGCC 1 cut(s) 581
KroI GCCGGC 1 cut(s) 1037
KroNI GCCGGC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 5 cut(s) 115, 429, 651, 744, 856
LmnI GCTCC 7 cut(s) 85, 102, 129, 212, 229, 494, 841
Lsp1109I GCAGC 1 cut(s) 1098
LweI GCATC 1 cut(s) 549
MaeI CTAG 1 cut(s) 959
MaeII ACGT 2 cut(s) 863, 893
MaeIII GTNAC 5 cut(s) 461, 815, 872, 931, 962
MalI GATC 5 cut(s) 117, 431, 653, 746, 858
MboI GATC 5 cut(s) 115, 429, 651, 744, 856
MboII GAAGA 4 cut(s) 395, 631, 679, 774
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 5 cut(s) 277, 285, 477, 604, 1083
Mly113I GGCGCC 1 cut(s) 582
MlyI GAGTC 3 cut(s) 137, 233, 437
MmeI TCCRAC 4 cut(s) 382, 465, 516, 533
MnlI CCTC 4 cut(s) 689, 750, 999, 1039
MroNI GCCGGC 1 cut(s) 1037
MseI TTAA 3 cut(s) 159, 258, 684
MslI CAYNNNNRTG 2 cut(s) 327, 653
MspI CCGG 9 cut(s) 127, 186, 530, 567, 611, 791, 832, 1038, 1069
MspR9I CCNGG 2 cut(s) 644, 1070
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 2 cut(s) 217, 353
MvaI CCWGG 1 cut(s) 644
MvnI CGCG 2 cut(s) 824, 930
MwoI GCNNNNNNNGC 2 cut(s) 705, 828
NaeI GCCGGC 1 cut(s) 1039
NarI GGCGCC 1 cut(s) 582
NciI CCSGG 1 cut(s) 1070
NcoI CCATGG 1 cut(s) 1095
NdeII GATC 5 cut(s) 115, 429, 651, 744, 856
NgoMIV GCCGGC 1 cut(s) 1037
NlaIII CATG 5 cut(s) 175, 239, 652, 658, 1099
NlaIV GGNNCC 5 cut(s) 81, 117, 131, 490, 583
NmeAIII GCCGAG 1 cut(s) 942
NmuCI GTSAC 5 cut(s) 461, 815, 872, 931, 962
NspI RCATGY 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 327
PauI GCGCGC 1 cut(s) 822
PctI GAATGC 2 cut(s) 217, 353
PdiI GCCGGC 1 cut(s) 1039
PfeI GAWTC 3 cut(s) 405, 588, 727
PflMI CCANNNNNTGG 1 cut(s) 532
PfoI TCCNGGA 1 cut(s) 642
PkrI GCNGC 2 cut(s) 1036, 1113
PleI GAGTC 3 cut(s) 137, 233, 437
PluTI GGCGCC 1 cut(s) 585
PpsI GAGTC 3 cut(s) 137, 233, 437
Ppu21I YACGTR 1 cut(s) 864
PpuMI RGGWCCY 1 cut(s) 68
PshAI GACNNNNGTC 1 cut(s) 813
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 642
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 5 cut(s) 81, 117, 131, 490, 583
PspPI GGNCC 4 cut(s) 68, 523, 1019, 1066
PspPPI RGGWCCY 1 cut(s) 68
PsuI RGATCY 1 cut(s) 115
PteI GCGCGC 1 cut(s) 822
RsaI GTAC 4 cut(s) 13, 741, 866, 994
RsaNI GTAC 4 cut(s) 12, 740, 865, 993
RseI CAYNNNNRTG 2 cut(s) 327, 653
Rsr2I CGGWCCG 1 cut(s) 1066
RsrII CGGWCCG 1 cut(s) 1066
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 159, 258, 684
SatI GCNGC 2 cut(s) 1035, 1112
Sau3AI GATC 5 cut(s) 115, 429, 651, 744, 856
Sau96I GGNCC 4 cut(s) 68, 523, 1019, 1066
SchI GAGTC 3 cut(s) 137, 233, 437
ScrFI CCNGG 2 cut(s) 644, 1070
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 1 cut(s) 549
SfcI CTRYAG 1 cut(s) 811
SfoI GGCGCC 1 cut(s) 583
SinI GGWCC 4 cut(s) 68, 523, 1019, 1066
SmiMI CAYNNNNRTG 2 cut(s) 327, 653
Sse9I AATT 5 cut(s) 277, 285, 477, 604, 1083
SsiI CCGC 5 cut(s) 189, 521, 986, 1032, 1035
SspDI GGCGCC 1 cut(s) 581
SspI AATATT 1 cut(s) 256
SspMI CTAG 1 cut(s) 959
StyD4I CCNGG 2 cut(s) 642, 1068
StyI CCWWGG 3 cut(s) 26, 100, 1095
TaaI ACNGT 8 cut(s) 16, 271, 311, 436, 547, 812, 869, 1066
TaiI ACGT 2 cut(s) 866, 896
TaqI TCGA 4 cut(s) 5, 138, 201, 422
TasI AATT 5 cut(s) 277, 285, 477, 604, 1083
TatI WGTACW 2 cut(s) 11, 739
TauI GCSGC 1 cut(s) 1037
TfiI GAWTC 3 cut(s) 405, 588, 727
Tru1I TTAA 3 cut(s) 159, 258, 684
Tru9I TTAA 3 cut(s) 159, 258, 684
TscAI CASTG 4 cut(s) 316, 509, 754, 874
TseFI GTSAC 5 cut(s) 461, 815, 872, 931, 962
TseI GCWGC 1 cut(s) 1111
Tsp45I GTSAC 5 cut(s) 461, 815, 872, 931, 962
TspDTI ATGAA 1 cut(s) 774
TspGWI ACGGA 2 cut(s) 219, 417
TspRI CASTG 4 cut(s) 316, 509, 754, 874
Van91I CCANNNNNTGG 1 cut(s) 532
VpaK11BI GGWCC 4 cut(s) 68, 523, 1019, 1066
XceI RCATGY 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.