RLG00000004922

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011526
Physical Location & Seq
Reverse (-)
63673516 .. 63674862
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004922

Sequence Viewer

Length: 1347 bp
ATGAAATCGACCAACATATATTTTGGCAGACTTTTGCTCTCATTGTTAGCATTCACCGTTGTAGCTGATCAATCCAATGTCTTTGATGTCACAAGCTCAAAATATGGTGGAAATCCCGACTCGGATATTTCAAAGGCAATGACAATGTCGTGGACAGATGCGTGTGCATCAACGTCGATGAGTACAGTACTTGTTCCAAGGGGAACATACGAGTTATTACAACAAGCAACATTTGAAGGTCCTTGTAAGGCTCCTATTCAGTTTCAGCTCCAAGGAACATTACAGGCTCCAAAAGACCGGAGCCAACTCGATGACTCTTGGGTTCAGTTTAAGAACTTGGACATGCTCACCTTGTCCGGCGGTGGAACTTTCGACGGACAAGGAGCGCATTCTTGGAAGGAGCACCATGACTCTACTCCAGTCGATATTAAGTTCAAAACCGTCAACAATTCCATAATTAGCGACATAACTTTTATGGACAGTGCAAAGTTCCACATTAGTGTTCACGACTGCAACAACGTTGCATTCCAACGGCTCACCATAACAGGACCCGAAGATAGTCCTAACACCGACGGAATCCACATCTCCAGTTCGACCAAGATCACAGTCTCCGACTCCTACATTGCAACTGGTGACGATTGTGTTTCAATTGGCGATGGCTCCAACCAAATCCTAGTGTCCAATATTGCTTGCGGACCAGGTCATGGAATAAGCATAGGCAGTCTAGGAAAGTATCCAGATGAACAGCCGGTGGTCGGCATCACGGTCAAGAACTGCACACTTACCGGCACAACCAACGGCGCGAGAATCAAGACATGGCCCAATTCTCCGGCAACAAGCGTTGCTTGGGATATACTCTTCCAGGACATGATCATGGTGAATGTGAAGAACCCTATCCTCATTAACCAGAACTACTGCGACAAAGGCAAGTCGTGTCAGCTAACGATTCCATCCAAAGTACAGATCAGTGATGTCAGGTTCAAGAATATCAGGGGCTCGTCTTCATCTCCGGTAGCCGTCAAGCTGGACTGTAGTCACAGCGCGCCGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCACGTACAGTGGTGACGAAAAAGACAAAATCAGGTCCGAGTGTAATAATGTCAGGCCGAGACTTACTCGCGTGACTAGGGCTCTTGCTTGTACCAATCTAGGTGACATACCAATAGATAATTCACCGCCTCGTACTGATGATGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGCAATATTTTGGGGCAAAAACCGATGGTCCGGGTGATGGTGCTAATCAGTTCCACCATGGCTTTGTGGTTGCTGCACGGCTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

449

Amino Acids

48.35

Weight (kDa)

5.71

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 378 8.7e-96 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 704
AccII CGCG 3 cut(s) 803, 1043, 1149
AciI CCGC 5 cut(s) 360, 693, 1205, 1251, 1254
AclI AACGTT 1 cut(s) 519
AfaI GTAC 6 cut(s) 184, 189, 960, 1085, 1171, 1213
AfiI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1294
AgsI TTSAA 5 cut(s) 132, 236, 436, 648, 982
AjnI CCWGG 2 cut(s) 697, 861
AjuI GAANNNNNNNTTGG 2 cut(s) 1183, 1215
AleI CACNNNNGTG 1 cut(s) 498
AluBI AGCT 6 cut(s) 65, 96, 268, 940, 1024, 1063
AluI AGCT 6 cut(s) 65, 96, 268, 940, 1024, 1063
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 2 cut(s) 613, 1132
AoxI GGCC 2 cut(s) 818, 1133
ApeKI GCWGC 1 cut(s) 1330
AspLEI GCGC 4 cut(s) 388, 803, 1043, 1045
AspS9I GGNCC 7 cut(s) 239, 548, 695, 819, 1113, 1238, 1285
AsuC2I CCSGG 1 cut(s) 1289
AsuHPI GGTGA 9 cut(s) 46, 340, 529, 644, 889, 1103, 1193, 1194, 1303
AvaII GGWCC 6 cut(s) 239, 548, 695, 1113, 1238, 1285
BanII GRGCYC 2 cut(s) 998, 1162
BbsI GAAGAC 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 1317
BccI CCATC 5 cut(s) 650, 958, 1220, 1276, 1288
BceAI ACGGC 4 cut(s) 548, 814, 1001, 1030
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 2 cut(s) 699, 863
BciVI GTATCC 1 cut(s) 744
BclI TGATCA 2 cut(s) 67, 870
BcnI CCSGG 1 cut(s) 1289
BcoDI GTCTC 2 cut(s) 613, 1132
BfaI CTAG 4 cut(s) 674, 725, 1155, 1178
BfmI CTRYAG 1 cut(s) 1030
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 2 cut(s) 1254, 1331
BlsI GCNGC 2 cut(s) 1255, 1332
BmcAI AGTACT 1 cut(s) 189
Bme1390I CCNGG 3 cut(s) 699, 863, 1289
Bme18I GGWCC 6 cut(s) 239, 548, 695, 1113, 1238, 1285
BmgT120I GGNCC 7 cut(s) 239, 548, 695, 819, 1113, 1238, 1285
BmiI GGNNCC 5 cut(s) 252, 288, 302, 550, 661
BmrFI CCNGG 3 cut(s) 699, 863, 1289
BmsI GCATC 3 cut(s) 148, 176, 768
BoxI GACNNNNGTC 1 cut(s) 1032
BpiI GAAGAC 1 cut(s) 993
BplI GAGNNNNNCTC 2 cut(s) 1129, 1161
BpmI CTGGAG 2 cut(s) 402, 571
BpuMI CCSGG 1 cut(s) 1289
BsaAI YACGTR 1 cut(s) 1083
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 197, 271, 1314
BsaWI WCCGGW 2 cut(s) 297, 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1294
Bse118I RCCGGY 3 cut(s) 748, 785, 1256
Bse1I ACTGG 3 cut(s) 419, 588, 634
Bse3DI GCAATG 2 cut(s) 144, 621
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 2 cut(s) 699, 863
BseDI CCNNGG 3 cut(s) 197, 271, 1314
BseGI GGATG 2 cut(s) 950, 1244
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1294
BseMI GCAATG 2 cut(s) 144, 621
BseNI ACTGG 3 cut(s) 419, 588, 634
BsePI GCGCGC 1 cut(s) 1041
BseXI GCAGC 1 cut(s) 1317
BsgI GTGCAG 2 cut(s) 760, 1316
Bsh1236I CGCG 3 cut(s) 803, 1043, 1149
BshFI GGCC 2 cut(s) 820, 1135
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1257, 1288
BslI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1294
BsmAI GTCTC 2 cut(s) 613, 1132
BsmI GAATGC 3 cut(s) 50, 388, 524
BsnI GGCC 2 cut(s) 820, 1135
Bsp1286I GDGCHC 3 cut(s) 405, 998, 1162
Bsp143I GATC 5 cut(s) 67, 600, 870, 963, 1075
Bsp19I CCATGG 1 cut(s) 1314
BspACI CCGC 5 cut(s) 360, 693, 1205, 1251, 1254
BspANI GGCC 2 cut(s) 820, 1135
BspFNI CGCG 3 cut(s) 803, 1043, 1149
BspLI GGNNCC 5 cut(s) 252, 288, 302, 550, 661
BsrDI GCAATG 2 cut(s) 144, 621
BsrFI RCCGGY 3 cut(s) 748, 785, 1256
BsrI ACTGG 3 cut(s) 419, 588, 634
BssAI RCCGGY 3 cut(s) 748, 785, 1256
BssECI CCNNGG 3 cut(s) 197, 271, 1314
BssHII GCGCGC 1 cut(s) 1041
BssMI GATC 5 cut(s) 67, 600, 870, 963, 1075
BssT1I CCWWGG 3 cut(s) 197, 271, 1314
Bst2UI CCWGG 2 cut(s) 699, 863
Bst4CI ACNGT 8 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088
Bst6I CTCTTC 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 1083
BstC8I GCNNGC 3 cut(s) 691, 1043, 1258
BstDSI CCRYGG 1 cut(s) 1314
BstF5I GGATG 2 cut(s) 950, 1244
BstFNI CGCG 3 cut(s) 803, 1043, 1149
BstHHI GCGC 4 cut(s) 388, 803, 1043, 1045
BstKTI GATC 5 cut(s) 70, 603, 873, 966, 1078
BstMAI GTCTC 2 cut(s) 613, 1132
BstMBI GATC 5 cut(s) 67, 600, 870, 963, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 924, 1047
BstNI CCWGG 2 cut(s) 699, 863
BstNSI RCATGY 1 cut(s) 346
BstPAI GACNNNNGTC 1 cut(s) 1032
BstSCI CCNGG 3 cut(s) 697, 861, 1287
BstSFI CTRYAG 1 cut(s) 1030
BstUI CGCG 3 cut(s) 803, 1043, 1149
BstV1I GCAGC 1 cut(s) 1317
BstV2I GAAGAC 1 cut(s) 993
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 2 cut(s) 820, 1135
BtgI CCRYGG 1 cut(s) 1314
BtgZI GCGATG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 950, 1244
BtsIMutI CAGTG 3 cut(s) 487, 973, 1093
Cac8I GCNNGC 3 cut(s) 691, 1043, 1258
CfoI GCGC 4 cut(s) 388, 803, 1043, 1045
Cfr10I RCCGGY 3 cut(s) 748, 785, 1256
Cfr13I GGNCC 7 cut(s) 239, 548, 695, 819, 1113, 1238, 1285
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 6 cut(s) 183, 188, 959, 1084, 1170, 1212
CviAII CATG 7 cut(s) 343, 407, 704, 816, 868, 874, 1315
CviQI GTAC 6 cut(s) 183, 188, 959, 1084, 1170, 1212
DpnI GATC 5 cut(s) 69, 602, 872, 965, 1077
DpnII GATC 5 cut(s) 67, 600, 870, 963, 1075
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
EciI GGCGGA 1 cut(s) 1240
Eco130I CCWWGG 3 cut(s) 197, 271, 1314
Eco24I GRGCYC 2 cut(s) 998, 1162
Eco47I GGWCC 6 cut(s) 239, 548, 695, 1113, 1238, 1285
EcoO109I RGGNCCY 2 cut(s) 239, 548
EcoRII CCWGG 2 cut(s) 697, 861
EcoT14I CCWWGG 3 cut(s) 197, 271, 1314
EcoT38I GRGCYC 2 cut(s) 998, 1162
ErhI CCWWGG 3 cut(s) 197, 271, 1314
FaeI CATG 7 cut(s) 346, 410, 707, 819, 871, 877, 1318
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FatI CATG 7 cut(s) 342, 406, 703, 815, 867, 873, 1314
FbaI TGATCA 2 cut(s) 67, 870
Fnu4HI GCNGC 2 cut(s) 1254, 1331
FokI GGATG 2 cut(s) 937, 1231
FriOI GRGCYC 2 cut(s) 998, 1162
Fsp4HI GCNGC 2 cut(s) 1254, 1331
FspBI CTAG 4 cut(s) 674, 725, 1155, 1178
GlaI GCGC 4 cut(s) 387, 802, 1042, 1044
GluI GCNGC 2 cut(s) 1254, 1331
GsuI CTGGAG 2 cut(s) 402, 571
HaeIII GGCC 2 cut(s) 820, 1135
HapII CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1257, 1288
HhaI GCGC 4 cut(s) 388, 803, 1043, 1045
Hin1II CATG 7 cut(s) 346, 410, 707, 819, 871, 877, 1318
Hin6I GCGC 4 cut(s) 386, 801, 1041, 1043
HinP1I GCGC 4 cut(s) 386, 801, 1041, 1043
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HinfI GANTC 7 cut(s) 119, 314, 410, 576, 614, 807, 946
HpaII CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1257, 1288
HphI GGTGA 9 cut(s) 46, 340, 529, 644, 889, 1103, 1193, 1194, 1303
Hpy166II GTNNAC 3 cut(s) 153, 445, 505
Hpy188I TCNGA 4 cut(s) 124, 613, 1069, 1117
Hpy188III TCNNGA 6 cut(s) 116, 506, 737, 769, 811, 982
Hpy8I GTNNAC 3 cut(s) 153, 445, 505
Hpy99I CGWCG 3 cut(s) 178, 377, 575
HpyAV CCTTC 2 cut(s) 230, 391
HpyCH4III ACNGT 8 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088
HpyCH4IV ACGT 3 cut(s) 173, 519, 1082
HpyCH4V TGCA 7 cut(s) 167, 485, 513, 524, 626, 777, 1333
HpyF10VI GCNNNNNNNGC 2 cut(s) 924, 1047
HpySE526I ACGT 3 cut(s) 173, 519, 1082
Hsp92II CATG 7 cut(s) 346, 410, 707, 819, 871, 877, 1318
HspAI GCGC 4 cut(s) 386, 801, 1041, 1043
KroI GCCGGC 1 cut(s) 1256
KroNI GCCGGC 1 cut(s) 1258
Ksp22I TGATCA 2 cut(s) 67, 870
Kzo9I GATC 5 cut(s) 67, 600, 870, 963, 1075
LmnI GCTCC 8 cut(s) 256, 273, 292, 300, 383, 400, 665, 1060
Lsp1109I GCAGC 1 cut(s) 1317
LweI GCATC 3 cut(s) 148, 176, 768
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 4 cut(s) 674, 725, 1155, 1178
MaeII ACGT 3 cut(s) 173, 519, 1082
MaeIII GTNAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
MalI GATC 5 cut(s) 69, 602, 872, 965, 1077
MboI GATC 5 cut(s) 67, 600, 870, 963, 1075
MboII GAAGA 4 cut(s) 566, 850, 898, 993
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 3 cut(s) 405, 998, 1162
MluCI AATT 5 cut(s) 448, 456, 648, 823, 1198
MlyI GAGTC 4 cut(s) 113, 308, 404, 608
MmeI TCCRAC 3 cut(s) 553, 636, 687
MnlI CCTC 3 cut(s) 908, 1218, 1258
MroNI GCCGGC 1 cut(s) 1256
MseI TTAA 3 cut(s) 330, 429, 903
MslI CAYNNNNRTG 2 cut(s) 498, 872
MspI CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1257, 1288
MspR9I CCNGG 3 cut(s) 699, 863, 1289
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 3 cut(s) 50, 388, 524
MvaI CCWGG 2 cut(s) 699, 863
MvnI CGCG 3 cut(s) 803, 1043, 1149
MwoI GCNNNNNNNGC 2 cut(s) 924, 1047
NaeI GCCGGC 1 cut(s) 1258
NciI CCSGG 1 cut(s) 1289
NcoI CCATGG 1 cut(s) 1314
NdeII GATC 5 cut(s) 67, 600, 870, 963, 1075
NgoMIV GCCGGC 1 cut(s) 1256
NlaIII CATG 7 cut(s) 346, 410, 707, 819, 871, 877, 1318
NlaIV GGNNCC 5 cut(s) 252, 288, 302, 550, 661
NmeAIII GCCGAG 1 cut(s) 1161
NmuCI GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
NspI RCATGY 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 498
PauI GCGCGC 1 cut(s) 1041
PctI GAATGC 3 cut(s) 50, 388, 524
PdiI GCCGGC 1 cut(s) 1258
PfeI GAWTC 3 cut(s) 576, 807, 946
PflFI GACNNNGTC 2 cut(s) 145, 699
PflMI CCANNNNNTGG 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 861
PkrI GCNGC 2 cut(s) 1255, 1332
PleI GAGTC 4 cut(s) 113, 308, 404, 608
PpsI GAGTC 4 cut(s) 113, 308, 404, 608
Ppu21I YACGTR 1 cut(s) 1083
PpuMI RGGWCCY 2 cut(s) 239, 548
PshAI GACNNNNGTC 1 cut(s) 1032
Psp1406I AACGTT 1 cut(s) 519
Psp5II RGGWCCY 2 cut(s) 239, 548
Psp6I CCWGG 2 cut(s) 697, 861
PspGI CCWGG 2 cut(s) 697, 861
PspN4I GGNNCC 5 cut(s) 252, 288, 302, 550, 661
PspPI GGNCC 7 cut(s) 239, 548, 695, 819, 1113, 1238, 1285
PspPPI RGGWCCY 2 cut(s) 239, 548
PsyI GACNNNGTC 2 cut(s) 145, 699
PteI GCGCGC 1 cut(s) 1041
RsaI GTAC 6 cut(s) 184, 189, 960, 1085, 1171, 1213
RsaNI GTAC 6 cut(s) 183, 188, 959, 1084, 1170, 1212
RseI CAYNNNNRTG 2 cut(s) 498, 872
SaqAI TTAA 3 cut(s) 330, 429, 903
SatI GCNGC 2 cut(s) 1254, 1331
Sau3AI GATC 5 cut(s) 67, 600, 870, 963, 1075
Sau96I GGNCC 7 cut(s) 239, 548, 695, 819, 1113, 1238, 1285
ScaI AGTACT 1 cut(s) 189
SchI GAGTC 4 cut(s) 113, 308, 404, 608
ScrFI CCNGG 3 cut(s) 699, 863, 1289
SduI GDGCHC 3 cut(s) 405, 998, 1162
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 3 cut(s) 148, 176, 768
SfcI CTRYAG 1 cut(s) 1030
SinI GGWCC 6 cut(s) 239, 548, 695, 1113, 1238, 1285
SmiMI CAYNNNNRTG 2 cut(s) 498, 872
Sse9I AATT 5 cut(s) 448, 456, 648, 823, 1198
SsiI CCGC 5 cut(s) 360, 693, 1205, 1251, 1254
SspI AATATT 2 cut(s) 685, 1264
SspMI CTAG 4 cut(s) 674, 725, 1155, 1178
StyD4I CCNGG 3 cut(s) 697, 861, 1287
StyI CCWWGG 3 cut(s) 197, 271, 1314
TaaI ACNGT 8 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088
TaiI ACGT 3 cut(s) 176, 522, 1085
TaqI TCGA 6 cut(s) 8, 176, 309, 372, 423, 593
TasI AATT 5 cut(s) 448, 456, 648, 823, 1198
TatI WGTACW 3 cut(s) 182, 187, 958
TauI GCSGC 1 cut(s) 1256
TfiI GAWTC 3 cut(s) 576, 807, 946
Tru1I TTAA 3 cut(s) 330, 429, 903
Tru9I TTAA 3 cut(s) 330, 429, 903
TscAI CASTG 3 cut(s) 487, 973, 1093
TseFI GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
TseI GCWGC 1 cut(s) 1330
Tsp45I GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
TspDTI ATGAA 3 cut(s) 17, 756, 993
TspGWI ACGGA 2 cut(s) 390, 588
TspRI CASTG 3 cut(s) 487, 973, 1093
Tth111I GACNNNGTC 2 cut(s) 145, 699
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 6 cut(s) 239, 548, 695, 1113, 1238, 1285
XceI RCATGY 1 cut(s) 346
XspI CTAG 4 cut(s) 674, 725, 1155, 1178
ZrmI AGTACT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.