Rw7G006070

Exopolygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011526
Physical Location & Seq
Forward (+)
5131353 .. 5132690
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G006070.1

Sequence Viewer

Length: 1338 bp
ATGAAATCGACCAACATATATTTTGGCAAACTTTTGCTCTCATTGTTAGCATTCACCGTTGTAGCTGATCAATCCAATGTCTTTGATGTGACTAGCTCAAAATATGGTGGAAAGCCTGACTCGGATATTTCAAAGGCAATGACAATGTCGTGGACAGATGCGTGTGCATCAATGTCGACGAGTACAGTCCTTGTTCCAAGGGGAACATACGAGTTATTACAAGAAGCAACATTTGAAGGTCCTTGTAAGGCTCCTGTTCAGTTTCAGCTCCAAGGAACATTGCAGGCTCCAAAAGACCGGAGCCAACTCGATGACTCTTGGGTTCAGTTTAAGAACTTGGACATGCTCACCTTGTCCGGCGGTGGAACTTTCGACGGACAAGGAGCGCATTCTTGGAAGGAGCACCATGACTCTACTCCAGTGGATATTAAGTTCAAAACCGTCAACAATTCCATAATTAGCGACATAACTTTTATGGACAGTGCAAAGTTCCACATTGGTGTTCACGACTGCAACAACGTTGCATTCCAACGGCTCACCATCACAGCACCCGAAGATAGTCCTAACACCGACGGAATCCACATCTCCAGTTCGACCAAGATCACAGTCTCCGACTCCTACATTGCAACTGGTGACGATTGTGTTTCAATTGGCGATGGCTCCAACCAAATCCTAGTGTCCAACATTGCTTGCGGACCAGGTCATGGAATAAGCATAGGCAGTCTAGGAAAGTATCCAGATGAACAGCCGGTGGTCGGCATCACGGTCAAGAACTGCACGCTTACCGGCACAACCAACGGCGCGAGAATCAAGACATGGCCCAATTCTCCGGCAACAAGCGTTGCTTGGGATATACTCTTCCAGGACATGATCATGGTGAATGTGAAGAACCCTATCCTCATTAACCAGAACTACTGCGACAAAGGCAAGTCGTGTAAGCTAATGATTCCATCCAAAGTACAGATCAGTGATGTGAGGTTCAAGAATATCAGGGGCTCGTCTTCATCTCCGGTAGCCGTCAAGCTGGACTGTAGTCACAGCGCGCCGTGCCGGAATGTGGAGCTGTCCGATATTGATCTCACGTACAGTGGTGACGAAAAAGACAAAATCACGTCCGAGTGTAATAATGTCAAGCCCAGACTTACTCGCGTGACTAAGGCTCTTGCTTGTGCCAATCTAGGTGACATACCAATAGATAATTCACCGCCTCATGATGATGGTGGTTTTGGACCAACATCCTCCGCCGCCGGCAATAGTTTGGGGCAAAACCCGACGGTCCGGGTGATGGTGCTAATTAGTTCCACCATGGCTTTGTGGTTGCTGCACGGCTTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

445

Amino Acids

47.78

Weight (kDa)

5.79

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 55 - 378 2.1e-95 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 1 cut(s) 176
AccII CGCG 3 cut(s) 803, 1043, 1149
AciI CCGC 5 cut(s) 360, 693, 1205, 1242, 1245
AclI AACGTT 1 cut(s) 519
AfaI GTAC 3 cut(s) 184, 960, 1085
AfiI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1285
AgsI TTSAA 5 cut(s) 132, 236, 436, 648, 982
AjiI CACGTC 1 cut(s) 1113
AjnI CCWGG 2 cut(s) 697, 861
AjuI GAANNNNNNNTTGG 2 cut(s) 1183, 1215
AleI CACNNNNGTG 1 cut(s) 498
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 6 cut(s) 65, 96, 268, 940, 1024, 1063
AluI AGCT 6 cut(s) 65, 96, 268, 940, 1024, 1063
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 613
AoxI GGCC 1 cut(s) 818
ApeKI GCWGC 1 cut(s) 1321
AspLEI GCGC 4 cut(s) 388, 803, 1043, 1045
AspS9I GGNCC 5 cut(s) 239, 695, 819, 1229, 1276
AsuC2I CCSGG 1 cut(s) 1280
AsuHPI GGTGA 9 cut(s) 46, 340, 529, 644, 889, 1103, 1193, 1194, 1294
AvaII GGWCC 4 cut(s) 239, 695, 1229, 1276
BanII GRGCYC 1 cut(s) 998
BbsI GAAGAC 1 cut(s) 993
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 1308
BccI CCATC 5 cut(s) 548, 650, 958, 1211, 1279
BceAI ACGGC 4 cut(s) 548, 814, 1001, 1030
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 2 cut(s) 699, 863
BciVI GTATCC 1 cut(s) 744
BclI TGATCA 2 cut(s) 67, 870
BcnI CCSGG 1 cut(s) 1280
BcoDI GTCTC 1 cut(s) 613
BfaI CTAG 4 cut(s) 93, 674, 725, 1178
BfmI CTRYAG 1 cut(s) 1030
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 2 cut(s) 1245, 1322
BlsI GCNGC 2 cut(s) 1246, 1323
Bme1390I CCNGG 3 cut(s) 699, 863, 1280
Bme18I GGWCC 4 cut(s) 239, 695, 1229, 1276
BmgBI CACGTC 1 cut(s) 1113
BmgT120I GGNCC 5 cut(s) 239, 695, 819, 1229, 1276
BmiI GGNNCC 4 cut(s) 252, 288, 302, 661
BmrFI CCNGG 3 cut(s) 699, 863, 1280
BmsI GCATC 3 cut(s) 148, 176, 768
BoxI GACNNNNGTC 1 cut(s) 1032
BpiI GAAGAC 1 cut(s) 993
BpmI CTGGAG 2 cut(s) 402, 571
BpuMI CCSGG 1 cut(s) 1280
BsaAI YACGTR 1 cut(s) 1083
BsaBI GATNNNNATC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 197, 271, 1305
BsaWI WCCGGW 2 cut(s) 297, 1009
Bsc4I CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1285
Bse118I RCCGGY 3 cut(s) 748, 785, 1247
Bse1I ACTGG 3 cut(s) 419, 588, 634
Bse3DI GCAATG 4 cut(s) 144, 278, 621, 684
Bse8I GATNNNNATC 1 cut(s) 1074
BseBI CCWGG 2 cut(s) 699, 863
BseDI CCNNGG 3 cut(s) 197, 271, 1305
BseGI GGATG 2 cut(s) 950, 1235
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1285
BseMI GCAATG 4 cut(s) 144, 278, 621, 684
BseNI ACTGG 3 cut(s) 419, 588, 634
BsePI GCGCGC 1 cut(s) 1041
BseXI GCAGC 1 cut(s) 1308
BsgI GTGCAG 2 cut(s) 760, 1307
Bsh1236I CGCG 3 cut(s) 803, 1043, 1149
BshFI GGCC 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1248, 1279
BslI CCNNNNNNNGG 4 cut(s) 704, 755, 1057, 1285
BsmAI GTCTC 1 cut(s) 613
BsmI GAATGC 3 cut(s) 50, 388, 524
BsnI GGCC 1 cut(s) 820
Bsp1286I GDGCHC 2 cut(s) 405, 998
Bsp143I GATC 5 cut(s) 67, 600, 870, 963, 1075
Bsp19I CCATGG 1 cut(s) 1305
BspACI CCGC 5 cut(s) 360, 693, 1205, 1242, 1245
BspANI GGCC 1 cut(s) 820
BspFNI CGCG 3 cut(s) 803, 1043, 1149
BspHI TCATGA 1 cut(s) 1210
BspLI GGNNCC 4 cut(s) 252, 288, 302, 661
BsrDI GCAATG 4 cut(s) 144, 278, 621, 684
BsrFI RCCGGY 3 cut(s) 748, 785, 1247
BsrI ACTGG 3 cut(s) 419, 588, 634
BssAI RCCGGY 3 cut(s) 748, 785, 1247
BssECI CCNNGG 3 cut(s) 197, 271, 1305
BssHII GCGCGC 1 cut(s) 1041
BssMI GATC 5 cut(s) 67, 600, 870, 963, 1075
BssT1I CCWWGG 3 cut(s) 197, 271, 1305
Bst2UI CCWGG 2 cut(s) 699, 863
Bst4CI ACNGT 9 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088, 1276
Bst6I CTCTTC 1 cut(s) 863
BstBAI YACGTR 1 cut(s) 1083
BstC8I GCNNGC 5 cut(s) 285, 691, 779, 1043, 1249
BstDEI CTNAG 1 cut(s) 1155
BstDSI CCRYGG 1 cut(s) 1305
BstF5I GGATG 2 cut(s) 950, 1235
BstFNI CGCG 3 cut(s) 803, 1043, 1149
BstHHI GCGC 4 cut(s) 388, 803, 1043, 1045
BstKTI GATC 5 cut(s) 70, 603, 873, 966, 1078
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 5 cut(s) 67, 600, 870, 963, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 924, 1047
BstNI CCWGG 2 cut(s) 699, 863
BstNSI RCATGY 1 cut(s) 346
BstPAI GACNNNNGTC 1 cut(s) 1032
BstSCI CCNGG 3 cut(s) 697, 861, 1278
BstSFI CTRYAG 1 cut(s) 1030
BstUI CGCG 3 cut(s) 803, 1043, 1149
BstV1I GCAGC 1 cut(s) 1308
BstV2I GAAGAC 1 cut(s) 993
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 1 cut(s) 820
BtgI CCRYGG 1 cut(s) 1305
BtgZI GCGATG 1 cut(s) 669
BtrI CACGTC 1 cut(s) 1113
BtsCI GGATG 2 cut(s) 950, 1235
BtsIMutI CAGTG 4 cut(s) 426, 487, 973, 1093
Cac8I GCNNGC 5 cut(s) 285, 691, 779, 1043, 1249
CciI TCATGA 1 cut(s) 1210
CfoI GCGC 4 cut(s) 388, 803, 1043, 1045
Cfr10I RCCGGY 3 cut(s) 748, 785, 1247
Cfr13I GGNCC 5 cut(s) 239, 695, 819, 1229, 1276
CpoI CGGWCCG 1 cut(s) 1276
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 3 cut(s) 183, 959, 1084
CspI CGGWCCG 1 cut(s) 1276
CviAII CATG 8 cut(s) 343, 407, 704, 816, 868, 874, 1211, 1306
CviQI GTAC 3 cut(s) 183, 959, 1084
DdeI CTNAG 1 cut(s) 1155
DpnI GATC 5 cut(s) 69, 602, 872, 965, 1077
DpnII GATC 5 cut(s) 67, 600, 870, 963, 1075
Eam1104I CTCTTC 1 cut(s) 863
EarI CTCTTC 1 cut(s) 863
EciI GGCGGA 1 cut(s) 1231
Eco130I CCWWGG 3 cut(s) 197, 271, 1305
Eco24I GRGCYC 1 cut(s) 998
Eco47I GGWCC 4 cut(s) 239, 695, 1229, 1276
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 697, 861
EcoT14I CCWWGG 3 cut(s) 197, 271, 1305
EcoT38I GRGCYC 1 cut(s) 998
ErhI CCWWGG 3 cut(s) 197, 271, 1305
FaeI CATG 8 cut(s) 346, 410, 707, 819, 871, 877, 1214, 1309
FalI AAGNNNNNCTT 2 cut(s) 829, 861
FatI CATG 8 cut(s) 342, 406, 703, 815, 867, 873, 1210, 1305
FbaI TGATCA 2 cut(s) 67, 870
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 2 cut(s) 1245, 1322
FokI GGATG 2 cut(s) 937, 1222
FriOI GRGCYC 1 cut(s) 998
Fsp4HI GCNGC 2 cut(s) 1245, 1322
FspBI CTAG 4 cut(s) 93, 674, 725, 1178
GlaI GCGC 4 cut(s) 387, 802, 1042, 1044
GluI GCNGC 2 cut(s) 1245, 1322
GsuI CTGGAG 2 cut(s) 402, 571
HaeIII GGCC 1 cut(s) 820
HapII CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1248, 1279
HhaI GCGC 4 cut(s) 388, 803, 1043, 1045
Hin1II CATG 8 cut(s) 346, 410, 707, 819, 871, 877, 1214, 1309
Hin6I GCGC 4 cut(s) 386, 801, 1041, 1043
HinP1I GCGC 4 cut(s) 386, 801, 1041, 1043
HincII GTYRAC 2 cut(s) 177, 445
HindII GTYRAC 2 cut(s) 177, 445
HinfI GANTC 7 cut(s) 119, 314, 410, 576, 614, 807, 946
HpaII CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1248, 1279
HphI GGTGA 9 cut(s) 46, 340, 529, 644, 889, 1103, 1193, 1194, 1294
Hpy166II GTNNAC 4 cut(s) 153, 177, 445, 505
Hpy188I TCNGA 4 cut(s) 124, 613, 1069, 1117
Hpy188III TCNNGA 6 cut(s) 506, 737, 769, 811, 982, 1211
Hpy8I GTNNAC 4 cut(s) 153, 177, 445, 505
Hpy99I CGWCG 4 cut(s) 181, 377, 575, 1276
HpyAV CCTTC 2 cut(s) 230, 391
HpyCH4III ACNGT 9 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088, 1276
HpyCH4IV ACGT 3 cut(s) 519, 1082, 1112
HpyCH4V TGCA 8 cut(s) 167, 283, 485, 513, 524, 626, 777, 1324
HpyF10VI GCNNNNNNNGC 2 cut(s) 924, 1047
HpyF3I CTNAG 1 cut(s) 1155
HpySE526I ACGT 3 cut(s) 519, 1082, 1112
Hsp92II CATG 8 cut(s) 346, 410, 707, 819, 871, 877, 1214, 1309
HspAI GCGC 4 cut(s) 386, 801, 1041, 1043
KroI GCCGGC 1 cut(s) 1247
KroNI GCCGGC 1 cut(s) 1249
Ksp22I TGATCA 2 cut(s) 67, 870
Kzo9I GATC 5 cut(s) 67, 600, 870, 963, 1075
LmnI GCTCC 8 cut(s) 256, 273, 292, 300, 383, 400, 665, 1060
Lsp1109I GCAGC 1 cut(s) 1308
LweI GCATC 3 cut(s) 148, 176, 768
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 4 cut(s) 93, 674, 725, 1178
MaeII ACGT 3 cut(s) 519, 1082, 1112
MaeIII GTNAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
MalI GATC 5 cut(s) 69, 602, 872, 965, 1077
MboI GATC 5 cut(s) 67, 600, 870, 963, 1075
MboII GAAGA 4 cut(s) 566, 850, 898, 993
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 2 cut(s) 405, 998
MluCI AATT 6 cut(s) 448, 456, 648, 823, 1198, 1293
MlyI GAGTC 4 cut(s) 113, 308, 404, 608
MmeI TCCRAC 4 cut(s) 553, 636, 687, 705
MnlI CCTC 4 cut(s) 908, 969, 1218, 1249
MroNI GCCGGC 1 cut(s) 1247
MseI TTAA 3 cut(s) 330, 429, 903
MslI CAYNNNNRTG 3 cut(s) 498, 872, 1215
MspI CCGG 9 cut(s) 298, 357, 749, 786, 830, 1010, 1051, 1248, 1279
MspR9I CCNGG 3 cut(s) 699, 863, 1280
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 3 cut(s) 50, 388, 524
MvaI CCWGG 2 cut(s) 699, 863
MvnI CGCG 3 cut(s) 803, 1043, 1149
MwoI GCNNNNNNNGC 2 cut(s) 924, 1047
NaeI GCCGGC 1 cut(s) 1249
NciI CCSGG 1 cut(s) 1280
NcoI CCATGG 1 cut(s) 1305
NdeII GATC 5 cut(s) 67, 600, 870, 963, 1075
NgoMIV GCCGGC 1 cut(s) 1247
NlaIII CATG 8 cut(s) 346, 410, 707, 819, 871, 877, 1214, 1309
NlaIV GGNNCC 4 cut(s) 252, 288, 302, 661
NmuCI GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
NspI RCATGY 1 cut(s) 346
OliI CACNNNNGTG 1 cut(s) 498
PagI TCATGA 1 cut(s) 1210
PauI GCGCGC 1 cut(s) 1041
PctI GAATGC 3 cut(s) 50, 388, 524
PdiI GCCGGC 1 cut(s) 1249
PfeI GAWTC 3 cut(s) 576, 807, 946
PflFI GACNNNGTC 2 cut(s) 145, 699
PflMI CCANNNNNTGG 1 cut(s) 704
PfoI TCCNGGA 1 cut(s) 861
PkrI GCNGC 2 cut(s) 1246, 1323
PleI GAGTC 4 cut(s) 113, 308, 404, 608
PpsI GAGTC 4 cut(s) 113, 308, 404, 608
Ppu21I YACGTR 1 cut(s) 1083
PpuMI RGGWCCY 1 cut(s) 239
PshAI GACNNNNGTC 1 cut(s) 1032
Psp1406I AACGTT 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 2 cut(s) 697, 861
PspGI CCWGG 2 cut(s) 697, 861
PspN4I GGNNCC 4 cut(s) 252, 288, 302, 661
PspPI GGNCC 5 cut(s) 239, 695, 819, 1229, 1276
PspPPI RGGWCCY 1 cut(s) 239
PsyI GACNNNGTC 2 cut(s) 145, 699
PteI GCGCGC 1 cut(s) 1041
RsaI GTAC 3 cut(s) 184, 960, 1085
RsaNI GTAC 3 cut(s) 183, 959, 1084
RseI CAYNNNNRTG 3 cut(s) 498, 872, 1215
Rsr2I CGGWCCG 1 cut(s) 1276
RsrII CGGWCCG 1 cut(s) 1276
SalI GTCGAC 1 cut(s) 175
SaqAI TTAA 3 cut(s) 330, 429, 903
SatI GCNGC 2 cut(s) 1245, 1322
Sau3AI GATC 5 cut(s) 67, 600, 870, 963, 1075
Sau96I GGNCC 5 cut(s) 239, 695, 819, 1229, 1276
SchI GAGTC 4 cut(s) 113, 308, 404, 608
ScrFI CCNGG 3 cut(s) 699, 863, 1280
SduI GDGCHC 2 cut(s) 405, 998
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 3 cut(s) 148, 176, 768
SfcI CTRYAG 1 cut(s) 1030
SinI GGWCC 4 cut(s) 239, 695, 1229, 1276
SmiMI CAYNNNNRTG 3 cut(s) 498, 872, 1215
Sse9I AATT 6 cut(s) 448, 456, 648, 823, 1198, 1293
SsiI CCGC 5 cut(s) 360, 693, 1205, 1242, 1245
SspMI CTAG 4 cut(s) 93, 674, 725, 1178
StyD4I CCNGG 3 cut(s) 697, 861, 1278
StyI CCWWGG 3 cut(s) 197, 271, 1305
TaaI ACNGT 9 cut(s) 58, 187, 442, 482, 607, 766, 1031, 1088, 1276
TaiI ACGT 3 cut(s) 522, 1085, 1115
TaqI TCGA 5 cut(s) 8, 176, 309, 372, 593
TasI AATT 6 cut(s) 448, 456, 648, 823, 1198, 1293
TatI WGTACW 2 cut(s) 182, 958
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 3 cut(s) 576, 807, 946
Tru1I TTAA 3 cut(s) 330, 429, 903
Tru9I TTAA 3 cut(s) 330, 429, 903
TscAI CASTG 4 cut(s) 426, 487, 973, 1093
TseFI GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
TseI GCWGC 1 cut(s) 1321
Tsp45I GTSAC 6 cut(s) 88, 632, 1034, 1091, 1150, 1181
TspDTI ATGAA 3 cut(s) 17, 756, 993
TspGWI ACGGA 2 cut(s) 390, 588
TspRI CASTG 4 cut(s) 426, 487, 973, 1093
Tth111I GACNNNGTC 2 cut(s) 145, 699
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 4 cut(s) 239, 695, 1229, 1276
XceI RCATGY 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 4 cut(s) 93, 674, 725, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.