FvH4_2g25351

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010723
Physical Location & Seq
Forward (+)
20606722 .. 20607381
660 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25351.t1

Sequence Viewer

Length: 345 bp
ATGGCAAAGTCCTCTTCCATTATTCTCTTCTTGGCCTTCTGCTTGTTCTTCACAGCCGTGATCATCGATGCAAGCAACGGCAGCGATCACGGGTTGAGCTTTGTTCCGGCCAAGTCCCGTTGCCAGGGTTCCATTGCAGAGTGCATGGCTGAGGATGAGTTTGACATGGACTCTGAGATCAACAGGCGTATCTTGGCCACCACAAAGTACATCAGCTATGGAGCTCTACAGAGGAACACTGTGCCATGCTCGCAAAGGGGTGCTTCCTACTACAACTGCAAGCCCGGGGCACAGGCTAACCCCTACAACCGTGGCTGCAGTGCCATCACTCGCTGCCGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

115

Amino Acids

12.45

Weight (kDa)

8.52

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 53 - 113 1.2e-30 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 108, 195
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 1 cut(s) 124
AjnI CCWGG 1 cut(s) 123
AleI CACNNNNGTG 1 cut(s) 56
AluBI AGCT 3 cut(s) 99, 216, 224
AluI AGCT 3 cut(s) 99, 216, 224
Alw21I GWGCWC 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 3 cut(s) 33, 108, 195
ApeKI GCWGC 3 cut(s) 81, 315, 333
AsuC2I CCSGG 2 cut(s) 285, 286
AvaI CYCGRG 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 292
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BalI TGGCCA 1 cut(s) 197
BanII GRGCYC 1 cut(s) 226
Bbv12I GWGCWC 1 cut(s) 226
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 93, 302, 320
BccI CCATC 1 cut(s) 332
BceAI ACGGC 3 cut(s) 41, 94, 321
BciT130I CCWGG 1 cut(s) 125
BclI TGATCA 1 cut(s) 60
BcnI CCSGG 2 cut(s) 285, 286
BfmI CTRYAG 2 cut(s) 227, 316
BisI GCNGC 3 cut(s) 82, 316, 334
BlsI GCNGC 3 cut(s) 83, 317, 335
Bme1390I CCNGG 3 cut(s) 125, 285, 286
BmeT110I CYCGRG 1 cut(s) 284
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 3 cut(s) 125, 285, 286
BmsI GCATC 1 cut(s) 58
Bpu10I CCTNAGC 1 cut(s) 150
BpuMI CCSGG 2 cut(s) 285, 286
Bsa29I ATCGAT 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 124, 284, 285, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 125
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 4 cut(s) 124, 284, 285, 310
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 2 cut(s) 141, 165
BseSI GKGCMC 1 cut(s) 292
BseXI GCAGC 3 cut(s) 93, 302, 320
BshFI GGCC 3 cut(s) 35, 110, 197
BshVI ATCGAT 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 2 cut(s) 107, 285
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 124
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 3 cut(s) 35, 110, 197
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 226, 292
Bsp143I GATC 3 cut(s) 60, 85, 177
BspANI GGCC 3 cut(s) 35, 110, 197
BspCNI CTCAG 2 cut(s) 142, 166
BspDI ATCGAT 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 132
BssECI CCNNGG 4 cut(s) 124, 284, 285, 310
BssMI GATC 3 cut(s) 60, 85, 177
Bst2UI CCWGG 1 cut(s) 125
Bst4CI ACNGT 2 cut(s) 241, 311
Bst6I CTCTTC 2 cut(s) 19, 32
BstC8I GCNNGC 3 cut(s) 73, 251, 281
BstDEI CTNAG 2 cut(s) 150, 174
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 1 cut(s) 160
BstKTI GATC 3 cut(s) 63, 88, 180
BstMBI GATC 3 cut(s) 60, 85, 177
BstMWI GCNNNNNNNGC 2 cut(s) 81, 250
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 3 cut(s) 123, 283, 284
BstSFI CTRYAG 2 cut(s) 227, 316
BstSLI GKGCMC 1 cut(s) 292
BstV1I GCAGC 3 cut(s) 93, 302, 320
Bsu15I ATCGAT 1 cut(s) 66
BsuRI GGCC 3 cut(s) 35, 110, 197
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 237, 325
Cac8I GCNNGC 3 cut(s) 73, 251, 281
Cfr9I CCCGGG 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 66
Csp6I GTAC 1 cut(s) 208
CviAII CATG 3 cut(s) 145, 166, 246
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 150, 174
DpnI GATC 3 cut(s) 62, 87, 179
DpnII GATC 3 cut(s) 60, 85, 177
EaeI YGGCCR 2 cut(s) 108, 195
Eam1104I CTCTTC 2 cut(s) 19, 32
EarI CTCTTC 2 cut(s) 19, 32
Ecl136II GAGCTC 1 cut(s) 224
Eco24I GRGCYC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 224
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 226
FaeI CATG 3 cut(s) 148, 169, 249
FaiI YATR 4 cut(s) 146, 167, 219, 247
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 144, 165, 245
FbaI TGATCA 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 82, 316, 334
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 3 cut(s) 82, 316, 334
GluI GCNGC 3 cut(s) 82, 316, 334
HaeIII GGCC 3 cut(s) 35, 110, 197
HapII CCGG 2 cut(s) 107, 285
Hin1II CATG 3 cut(s) 148, 169, 249
HinfI GANTC 1 cut(s) 170
HpaII CCGG 2 cut(s) 107, 285
Hpy188I TCNGA 1 cut(s) 175
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 241, 311
HpyCH4V TGCA 5 cut(s) 71, 137, 144, 279, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 250
HpyF3I CTNAG 2 cut(s) 150, 174
Hsp92II CATG 3 cut(s) 148, 169, 249
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 3 cut(s) 60, 85, 177
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 110, 120, 137, 169, 278, 298
Lsp1109I GCAGC 3 cut(s) 93, 302, 320
LweI GCATC 1 cut(s) 58
MalI GATC 3 cut(s) 62, 87, 179
MboI GATC 3 cut(s) 60, 85, 177
MboII GAAGA 3 cut(s) 6, 19, 40
MhlI GDGCHC 2 cut(s) 226, 292
MlsI TGGCCA 1 cut(s) 197
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 164
MnlI CCTC 3 cut(s) 22, 145, 225
Mox20I TGGCCA 1 cut(s) 197
MscI TGGCCA 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 2 cut(s) 107, 285
MspR9I CCNGG 3 cut(s) 125, 285, 286
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 81, 250
NciI CCSGG 2 cut(s) 285, 286
NdeII GATC 3 cut(s) 60, 85, 177
NlaIII CATG 3 cut(s) 148, 169, 249
NlaIV GGNNCC 1 cut(s) 130
OliI CACNNNNGTG 1 cut(s) 56
PkrI GCNGC 3 cut(s) 83, 317, 335
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
Psp124BI GAGCTC 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 130
PstI CTGCAG 1 cut(s) 320
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 226
SatI GCNGC 3 cut(s) 82, 316, 334
Sau3AI GATC 3 cut(s) 60, 85, 177
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 125, 285, 286
SduI GDGCHC 2 cut(s) 226, 292
SetI ASST 3 cut(s) 101, 218, 226
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 2 cut(s) 227, 316
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 56
SstI GAGCTC 1 cut(s) 226
StyD4I CCNGG 3 cut(s) 123, 283, 284
TaaI ACNGT 2 cut(s) 241, 311
TaqI TCGA 1 cut(s) 66
TatI WGTACW 1 cut(s) 207
TscAI CASTG 2 cut(s) 244, 325
TseI GCWGC 3 cut(s) 81, 315, 333
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 2 cut(s) 244, 325
XmaI CCCGGG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.