pycom03g22590

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010723
Physical Location & Seq
Forward (+)
22674308 .. 22674649
342 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22590.1

Sequence Viewer

Length: 342 bp
ATGGCCAATTCCTCCGCCATTATCTTTTCCTTGGCCCTCGTCACCGCTCTGATCATCTCCACTGACGCAGGCGGTGATCACAGGGTGAGCTGGGTTCCGCTGAGACCCCGCTGCGAGGGTTCGGTAGCAGAGTGCATGGATGATCATGATGATGAGTTTGGCATGGACTCAGAGATCAGCAGGCGCATCTTGGCCACCTCACAGTACATAAGCTATGGAGCTCTGCAGAGAAACACTGTGCCTTGCTCTCACAGAGGTGCTTCCTACTACAACTGCAAGTCGGGTGCCCAGAGCAACCCCTACAAACGCGGATGCAGTTCTATTGCCCGTTGCCGGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

114

Amino Acids

12.28

Weight (kDa)

7.62

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 113 5.3e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AccBSI CCGCTC 1 cut(s) 47
AccII CGCG 1 cut(s) 309
AciI CCGC 6 cut(s) 15, 45, 72, 98, 109, 309
AcoI YGGCCR 2 cut(s) 3, 192
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 3 cut(s) 115, 333, 334
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 3 cut(s) 90, 213, 221
AluI AGCT 3 cut(s) 90, 213, 221
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 3 cut(s) 3, 33, 192
ApeKI GCWGC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 3 cut(s) 34, 86, 97
BaeGI GKGCMC 1 cut(s) 289
BalI TGGCCA 2 cut(s) 5, 194
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 98
BclI TGATCA 3 cut(s) 51, 76, 142
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 224
BisI GCNGC 1 cut(s) 112
BlsI GCNGC 1 cut(s) 113
Bme1390I CCNGG 1 cut(s) 335
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 2 cut(s) 96, 286
BmrFI CCNGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 195, 302
BpuMI CCSGG 1 cut(s) 335
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 30, 334
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 333, 334
BseDI CCNNGG 2 cut(s) 30, 334
BseGI GGATG 2 cut(s) 145, 317
BseLI CCNNNNNNNGG 3 cut(s) 115, 333, 334
BseMII CTCAG 2 cut(s) 92, 183
BseSI GKGCMC 1 cut(s) 289
BseXI GCAGC 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 5, 35, 194
BshNI GGYRCC 1 cut(s) 284
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 334
BslI CCNNNNNNNGG 3 cut(s) 115, 333, 334
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 3 cut(s) 5, 35, 194
Bso31I GGTCTC 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 223, 289
Bsp143I GATC 4 cut(s) 51, 76, 142, 174
BspACI CCGC 6 cut(s) 15, 45, 72, 98, 109, 309
BspANI GGCC 3 cut(s) 5, 35, 194
BspCNI CTCAG 2 cut(s) 93, 182
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 96, 286
BspMAI CTGCAG 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 47
BssECI CCNNGG 2 cut(s) 30, 334
BssMI GATC 4 cut(s) 51, 76, 142, 174
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 204, 238
BstC8I GCNNGC 2 cut(s) 70, 182
BstDEI CTNAG 2 cut(s) 101, 169
BstF5I GGATG 2 cut(s) 145, 317
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 4 cut(s) 54, 79, 145, 177
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 4 cut(s) 51, 76, 142, 174
BstSCI CCNGG 1 cut(s) 333
BstSFI CTRYAG 1 cut(s) 224
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 98
BsuRI GGCC 3 cut(s) 5, 35, 194
BtsCI GGATG 2 cut(s) 145, 317
BtsIMutI CAGTG 2 cut(s) 60, 234
Cac8I GCNNGC 2 cut(s) 70, 182
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 136, 146, 163
CviJI RGCY 6 cut(s) 5, 35, 90, 194, 213, 221
CviKI_1 RGCY 6 cut(s) 5, 35, 90, 194, 213, 221
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 2 cut(s) 101, 169
DpnI GATC 4 cut(s) 53, 78, 144, 176
DpnII GATC 4 cut(s) 51, 76, 142, 174
DraIII CACNNNGTG 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 3, 192
EciI GGCGGA 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 3 cut(s) 139, 149, 166
FaiI YATR 5 cut(s) 137, 147, 164, 209, 216
FatI CATG 3 cut(s) 135, 145, 162
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 3 cut(s) 51, 76, 142
Fnu4HI GCNGC 1 cut(s) 112
FokI GGATG 2 cut(s) 152, 324
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 112
GlaI GCGC 1 cut(s) 185
GluI GCNGC 1 cut(s) 112
GsaI CCCAGC 1 cut(s) 94
HaeIII GGCC 3 cut(s) 5, 35, 194
HapII CCGG 1 cut(s) 334
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 186
Hin1II CATG 3 cut(s) 139, 149, 166
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 1 cut(s) 167
HpaII CCGG 1 cut(s) 334
HphI GGTGA 3 cut(s) 34, 86, 97
Hpy188I TCNGA 2 cut(s) 51, 172
Hpy188III TCNNGA 1 cut(s) 146
HpyCH4III ACNGT 2 cut(s) 204, 238
HpyCH4V TGCA 4 cut(s) 135, 226, 276, 315
HpyF3I CTNAG 2 cut(s) 101, 169
Hsp92II CATG 3 cut(s) 139, 149, 166
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 3 cut(s) 51, 76, 142
Kzo9I GATC 4 cut(s) 51, 76, 142, 174
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 5 cut(s) 54, 67, 76, 166, 302
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 2 cut(s) 195, 302
MaeIII GTNAC 1 cut(s) 40
MalI GATC 4 cut(s) 53, 78, 144, 176
MbiI CCGCTC 1 cut(s) 47
MboI GATC 4 cut(s) 51, 76, 142, 174
MhlI GDGCHC 2 cut(s) 223, 289
MlsI TGGCCA 2 cut(s) 5, 194
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 2 cut(s) 5, 194
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 5 cut(s) 22, 47, 109, 208, 248
Mox20I TGGCCA 2 cut(s) 5, 194
MscI TGGCCA 2 cut(s) 5, 194
MslI CAYNNNNRTG 2 cut(s) 150, 255
Msp20I TGGCCA 2 cut(s) 5, 194
MspA1I CMGCKG 2 cut(s) 100, 111
MspI CCGG 1 cut(s) 334
MspR9I CCNGG 1 cut(s) 335
MvnI CGCG 1 cut(s) 309
NciI CCSGG 1 cut(s) 335
NdeII GATC 4 cut(s) 51, 76, 142, 174
NlaIII CATG 3 cut(s) 139, 149, 166
NlaIV GGNNCC 2 cut(s) 96, 286
NmuCI GTSAC 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 255
PagI TCATGA 1 cut(s) 145
PkrI GCNGC 1 cut(s) 113
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 2 cut(s) 96, 286
PspPI GGNCC 1 cut(s) 34
PstI CTGCAG 1 cut(s) 228
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 2 cut(s) 150, 255
SacI GAGCTC 1 cut(s) 223
SatI GCNGC 1 cut(s) 112
Sau3AI GATC 4 cut(s) 51, 76, 142, 174
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 2 cut(s) 223, 289
SetI ASST 5 cut(s) 92, 200, 215, 223, 259
SfaNI GCATC 2 cut(s) 195, 302
SfcI CTRYAG 1 cut(s) 224
SmiMI CAYNNNNRTG 2 cut(s) 150, 255
Sse9I AATT 1 cut(s) 7
SsiI CCGC 6 cut(s) 15, 45, 72, 98, 109, 309
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 333
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 204, 238
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 204
TscAI CASTG 2 cut(s) 67, 241
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 111
Tsp45I GTSAC 1 cut(s) 40
TspRI CASTG 2 cut(s) 67, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.