pycom11g26630

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0010723
Physical Location & Seq
Forward (+)
29131682 .. 29132099
418 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g26630.1

Sequence Viewer

Length: 378 bp
ATGACCTTCCAAAATACACACAACATACCAAAACCTATGGCCAATTCCTCCGCCATTATCTTTTCCTTGGCCTTCGTGGCTGCTTTGATCATCTCCGTTGATGCAAGCAGCGGTCATGGGGCGAGCTGGGTTCCGGTGAGACCCCGCTGTGAGGGTTCGGTAGCAGAGTGCATGGATGATCATGATGATGAGTTTGGCATGGACTCGGAGATCAGCAGGCGCATCTTGGCCACCTCACAATACATAAGCTATGGAGCTCTGCAGAGAAACACTGTACCTTGCTCTCAGAGAGGTGCTTCCTACTACAACTGCAAGCCAGGTGCTCAAAGCAACCCATACAACCGTGGATGCAGTGCTATTGCTCATTGCCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

126

Amino Acids

13.54

Weight (kDa)

6.87

Isoelectric Point (pI)

45.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 64 - 124 7e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 51, 111, 145
AcoI YGGCCR 2 cut(s) 39, 228
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 151
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 3 cut(s) 126, 249, 257
AluI AGCT 3 cut(s) 126, 249, 257
Alw21I GWGCWC 2 cut(s) 259, 325
Alw26I GTCTC 1 cut(s) 133
AoxI GGCC 3 cut(s) 39, 69, 228
ApeKI GCWGC 2 cut(s) 80, 108
AspLEI GCGC 1 cut(s) 222
AsuHPI GGTGA 1 cut(s) 148
BalI TGGCCA 2 cut(s) 41, 230
BanII GRGCYC 1 cut(s) 259
Bbv12I GWGCWC 2 cut(s) 259, 325
BbvI GCAGC 2 cut(s) 67, 120
BciT130I CCWGG 1 cut(s) 318
BclI TGATCA 2 cut(s) 87, 178
BcoDI GTCTC 1 cut(s) 133
BfmI CTRYAG 1 cut(s) 260
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 2 cut(s) 81, 109
BlsI GCNGC 2 cut(s) 82, 110
Bme1390I CCNGG 1 cut(s) 318
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 318
BmsI GCATC 3 cut(s) 91, 231, 338
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 66, 343
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 364
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 2 cut(s) 66, 343
BseGI GGATG 2 cut(s) 181, 353
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 1 cut(s) 299
BseXI GCAGC 2 cut(s) 67, 120
BseYI CCCAGC 1 cut(s) 126
BshFI GGCC 3 cut(s) 41, 71, 230
BsiHKAI GWGCWC 2 cut(s) 259, 325
BsiSI CCGG 2 cut(s) 134, 370
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 133
BsnI GGCC 3 cut(s) 41, 71, 230
Bso31I GGTCTC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 259, 325
Bsp143I GATC 3 cut(s) 87, 178, 210
BspACI CCGC 3 cut(s) 51, 111, 145
BspANI GGCC 3 cut(s) 41, 71, 230
BspCNI CTCAG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 132
BspMAI CTGCAG 1 cut(s) 264
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 364
BssECI CCNNGG 2 cut(s) 66, 343
BssMI GATC 3 cut(s) 87, 178, 210
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 274, 344
BstC8I GCNNGC 4 cut(s) 106, 124, 218, 314
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 2 cut(s) 181, 353
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 3 cut(s) 90, 181, 213
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 3 cut(s) 87, 178, 210
BstMWI GCNNNNNNNGC 1 cut(s) 77
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 316
BstSFI CTRYAG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 67, 120
BsuRI GGCC 3 cut(s) 41, 71, 230
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 2 cut(s) 181, 353
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 270, 358
Cac8I GCNNGC 4 cut(s) 106, 124, 218, 314
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 222
Csp6I GTAC 1 cut(s) 275
CviAII CATG 4 cut(s) 116, 172, 182, 199
CviJI RGCY 8 cut(s) 41, 71, 80, 126, 230, 249, 257, 316
CviKI_1 RGCY 8 cut(s) 41, 71, 80, 126, 230, 249, 257, 316
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 285
DpnI GATC 3 cut(s) 89, 180, 212
DpnII GATC 3 cut(s) 87, 178, 210
EaeI YGGCCR 2 cut(s) 39, 228
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 66
Eco24I GRGCYC 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 4 cut(s) 119, 175, 185, 202
FaiI YATR 9 cut(s) 26, 38, 117, 173, 183, 200, 245, 252, 337
FatI CATG 4 cut(s) 115, 171, 181, 198
FauI CCCGC 1 cut(s) 152
FbaI TGATCA 2 cut(s) 87, 178
Fnu4HI GCNGC 2 cut(s) 81, 109
FokI GGATG 2 cut(s) 188, 360
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 2 cut(s) 81, 109
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 81, 109
GsaI CCCAGC 1 cut(s) 130
HaeIII GGCC 3 cut(s) 41, 71, 230
HapII CCGG 2 cut(s) 134, 370
HhaI GCGC 1 cut(s) 222
Hin1II CATG 4 cut(s) 119, 175, 185, 202
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HinfI GANTC 1 cut(s) 203
HpaII CCGG 2 cut(s) 134, 370
HphI GGTGA 1 cut(s) 148
Hpy188I TCNGA 2 cut(s) 208, 288
Hpy188III TCNNGA 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 16, 82
HpyCH4III ACNGT 2 cut(s) 274, 344
HpyCH4V TGCA 5 cut(s) 104, 171, 262, 312, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 77
HpyF3I CTNAG 1 cut(s) 285
Hsp92II CATG 4 cut(s) 119, 175, 185, 202
HspAI GCGC 1 cut(s) 220
Ksp22I TGATCA 2 cut(s) 87, 178
Kzo9I GATC 3 cut(s) 87, 178, 210
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 5 cut(s) 112, 147, 202, 303, 330
Lsp1109I GCAGC 2 cut(s) 67, 120
LweI GCATC 3 cut(s) 91, 231, 338
MalI GATC 3 cut(s) 89, 180, 212
MboI GATC 3 cut(s) 87, 178, 210
MhlI GDGCHC 2 cut(s) 259, 325
MlsI TGGCCA 2 cut(s) 41, 230
MluCI AATT 1 cut(s) 43
MluNI TGGCCA 2 cut(s) 41, 230
MlyI GAGTC 1 cut(s) 197
MnlI CCTC 4 cut(s) 58, 145, 244, 284
Mox20I TGGCCA 2 cut(s) 41, 230
MscI TGGCCA 2 cut(s) 41, 230
MslI CAYNNNNRTG 1 cut(s) 186
Msp20I TGGCCA 2 cut(s) 41, 230
MspA1I CMGCKG 2 cut(s) 111, 147
MspI CCGG 2 cut(s) 134, 370
MspR9I CCNGG 1 cut(s) 318
MvaI CCWGG 1 cut(s) 318
MwoI GCNNNNNNNGC 1 cut(s) 77
NdeII GATC 3 cut(s) 87, 178, 210
NlaIII CATG 4 cut(s) 119, 175, 185, 202
NlaIV GGNNCC 1 cut(s) 132
PagI TCATGA 1 cut(s) 181
PkrI GCNGC 2 cut(s) 82, 110
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 259
Psp6I CCWGG 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 126
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 132
PstI CTGCAG 1 cut(s) 264
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 259
SatI GCNGC 2 cut(s) 81, 109
Sau3AI GATC 3 cut(s) 87, 178, 210
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 318
SduI GDGCHC 2 cut(s) 259, 325
SetI ASST 9 cut(s) 8, 37, 128, 236, 251, 259, 280, 295, 322
SfaNI GCATC 3 cut(s) 91, 231, 338
SfcI CTRYAG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 1 cut(s) 43
SsiI CCGC 3 cut(s) 51, 111, 145
SstI GAGCTC 1 cut(s) 259
StyD4I CCNGG 1 cut(s) 316
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 2 cut(s) 274, 344
TasI AATT 1 cut(s) 43
TscAI CASTG 2 cut(s) 277, 358
TseI GCWGC 2 cut(s) 80, 108
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 2 cut(s) 277, 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.