MD03G1284900.v1.1

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010723
Physical Location & Seq
Forward (+)
36495609 .. 36496183
575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1284900.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGCCAATTCCTCCACCATTATCTTTTCCTTGGCCCTCGTCGCCGCTCTGATCATCTCCGCTGATGCAGGGGGCGATCACAGGGTGAGCTGGGTTCCGCTGAGACCCGGCTGCGAGGGTTCGGTAGCAGAGTGCATGGATGATCATGATGTTGAGTTTGGCATGGACTCAGAGATCAGCAGGCGCATCTTGGCCACCTCACAGTACATAAGCTATGGAGCTCTGCAGAGAAACACCGTGCCTTGCTCTCACAGAGGTGCTTCCTACTACAACTGCAAGTCAGGTGCTCAGAGCAACCCCTACAAACGCGGATGCACTGCTATTGCCCGTTGCCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

114

Amino Acids

12.16

Weight (kDa)

7.62

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 112 2e-28 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 47
AccII CGCG 1 cut(s) 309
AciI CCGC 4 cut(s) 45, 60, 98, 309
AcoI YGGCCR 2 cut(s) 3, 192
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 333
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 3 cut(s) 90, 213, 221
AluI AGCT 3 cut(s) 90, 213, 221
Alw21I GWGCWC 2 cut(s) 223, 289
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 3 cut(s) 3, 33, 192
ApeKI GCWGC 1 cut(s) 111
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 97
BalI TGGCCA 2 cut(s) 5, 194
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 2 cut(s) 223, 289
BbvI GCAGC 1 cut(s) 98
BclI TGATCA 2 cut(s) 51, 142
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 224
BisI GCNGC 2 cut(s) 45, 112
BlsI GCNGC 2 cut(s) 46, 113
Bme1390I CCNGG 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 3 cut(s) 55, 195, 302
BpuMI CCSGG 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 333
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 2 cut(s) 145, 317
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMII CTCAG 3 cut(s) 92, 183, 302
BseXI GCAGC 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 5, 35, 194
BsiHKAI GWGCWC 2 cut(s) 223, 289
BsiSI CCGG 2 cut(s) 108, 334
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 3 cut(s) 5, 35, 194
Bso31I GGTCTC 1 cut(s) 97
Bsp1286I GDGCHC 2 cut(s) 223, 289
Bsp143I GATC 4 cut(s) 51, 76, 142, 174
BspACI CCGC 4 cut(s) 45, 60, 98, 309
BspANI GGCC 3 cut(s) 5, 35, 194
BspCNI CTCAG 3 cut(s) 93, 182, 301
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 4 cut(s) 51, 76, 142, 174
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 204, 238
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 3 cut(s) 101, 169, 288
BstF5I GGATG 2 cut(s) 145, 317
BstFNI CGCG 1 cut(s) 309
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 4 cut(s) 54, 79, 145, 177
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 4 cut(s) 51, 76, 142, 174
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 224
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 1 cut(s) 98
BsuRI GGCC 3 cut(s) 5, 35, 194
BtsCI GGATG 2 cut(s) 145, 317
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 182
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 1 cut(s) 205
CviAII CATG 3 cut(s) 136, 146, 163
CviJI RGCY 7 cut(s) 5, 35, 90, 111, 194, 213, 221
CviKI_1 RGCY 7 cut(s) 5, 35, 90, 111, 194, 213, 221
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 3 cut(s) 101, 169, 288
DpnI GATC 4 cut(s) 53, 78, 144, 176
DpnII GATC 4 cut(s) 51, 76, 142, 174
DraIII CACNNNGTG 1 cut(s) 85
EaeI YGGCCR 2 cut(s) 3, 192
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 3 cut(s) 139, 149, 166
FaiI YATR 5 cut(s) 137, 147, 164, 209, 216
FatI CATG 3 cut(s) 135, 145, 162
FbaI TGATCA 2 cut(s) 51, 142
Fnu4HI GCNGC 2 cut(s) 45, 112
FokI GGATG 2 cut(s) 152, 324
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 2 cut(s) 45, 112
GlaI GCGC 1 cut(s) 185
GluI GCNGC 2 cut(s) 45, 112
GsaI CCCAGC 1 cut(s) 94
HaeIII GGCC 3 cut(s) 5, 35, 194
HapII CCGG 2 cut(s) 108, 334
HhaI GCGC 1 cut(s) 186
Hin1II CATG 3 cut(s) 139, 149, 166
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 1 cut(s) 167
HpaII CCGG 2 cut(s) 108, 334
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 3 cut(s) 51, 172, 291
Hpy188III TCNNGA 1 cut(s) 146
Hpy99I CGWCG 1 cut(s) 44
HpyCH4III ACNGT 2 cut(s) 204, 238
HpyCH4V TGCA 5 cut(s) 68, 135, 226, 276, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 101, 169, 288
Hsp92II CATG 3 cut(s) 139, 149, 166
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 2 cut(s) 51, 142
Kzo9I GATC 4 cut(s) 51, 76, 142, 174
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 6 cut(s) 54, 67, 76, 121, 166, 267
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 3 cut(s) 55, 195, 302
MalI GATC 4 cut(s) 53, 78, 144, 176
MbiI CCGCTC 1 cut(s) 47
MboI GATC 4 cut(s) 51, 76, 142, 174
MhlI GDGCHC 2 cut(s) 223, 289
MlsI TGGCCA 2 cut(s) 5, 194
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 2 cut(s) 5, 194
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 5 cut(s) 22, 47, 109, 208, 248
Mox20I TGGCCA 2 cut(s) 5, 194
MscI TGGCCA 2 cut(s) 5, 194
MslI CAYNNNNRTG 1 cut(s) 255
Msp20I TGGCCA 2 cut(s) 5, 194
MspA1I CMGCKG 2 cut(s) 62, 100
MspI CCGG 2 cut(s) 108, 334
MspR9I CCNGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 51, 76, 142, 174
NlaIII CATG 3 cut(s) 139, 149, 166
NlaIV GGNNCC 1 cut(s) 96
OliI CACNNNNGTG 1 cut(s) 255
PagI TCATGA 1 cut(s) 145
PkrI GCNGC 2 cut(s) 46, 113
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 1 cut(s) 34
PstI CTGCAG 1 cut(s) 228
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
RseI CAYNNNNRTG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 223
SatI GCNGC 2 cut(s) 45, 112
Sau3AI GATC 4 cut(s) 51, 76, 142, 174
Sau96I GGNCC 1 cut(s) 34
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 2 cut(s) 223, 289
SetI ASST 6 cut(s) 92, 200, 215, 223, 259, 286
SfaNI GCATC 3 cut(s) 55, 195, 302
SfcI CTRYAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 1 cut(s) 7
SsiI CCGC 4 cut(s) 45, 60, 98, 309
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 204, 238
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 47
TscAI CASTG 1 cut(s) 322
TseI GCWGC 1 cut(s) 111
TspRI CASTG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.