MD16G1025500.v1.1

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010723
Physical Location & Seq
Reverse (-)
1826589 .. 1826936
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1025500.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCTAATTCCTCTGCGATTATCTTTTCGTTGATTTTCGTGGCCGCTCTGGTCATCTCCGTTGATGCAGGCGGCGATCATGGGGTGAGCTGGGTTCCAGTGAGACCTCGCTGCGAGGGTTCTGTAGCAGAGTGCATGGATGATCGTGATGATGAGTTTGGCATGGACTCGGAGATTAGCAGACGCATCTTGGCCACCTCACGAAACATAAGCTATGAAGCGCTGCAGAGAAACACTGTTCCTTGCTCTCAGAGAGGTGCTTCCTCCAACAACTGCAAGCCGGGTGCTCAGAATCAGAGCAACCCCTACAACCACGGATGCAGTGCCAATGCTCGTTGCCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

116

Amino Acids

12.37

Weight (kDa)

6.05

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 114 9.6e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 47
AciI CCGC 2 cut(s) 45, 72
AcoI YGGCCR 2 cut(s) 42, 192
AfeI AGCGCT 1 cut(s) 222
AluBI AGCT 2 cut(s) 90, 213
AluI AGCT 2 cut(s) 90, 213
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 97
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 2 cut(s) 42, 192
ApeKI GCWGC 2 cut(s) 111, 223
AspLEI GCGC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 97
BalI TGGCCA 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 289
BbvI GCAGC 2 cut(s) 98, 210
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 2 cut(s) 123, 224
BfoI RGCGCY 1 cut(s) 224
BisI GCNGC 4 cut(s) 45, 73, 112, 224
BlsI GCNGC 4 cut(s) 46, 74, 113, 225
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 55, 195, 308
BpuMI CCSGG 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 313
Bse1I ACTGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 313
BseGI GGATG 2 cut(s) 145, 323
BseMII CTCAG 2 cut(s) 263, 302
BseNI ACTGG 1 cut(s) 98
BseXI GCAGC 2 cut(s) 98, 210
BseYI CCCAGC 1 cut(s) 90
BshFI GGCC 2 cut(s) 44, 194
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 2 cut(s) 281, 340
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 2 cut(s) 44, 194
Bso31I GGTCTC 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 76, 142
BspACI CCGC 2 cut(s) 45, 72
BspANI GGCC 2 cut(s) 44, 194
BspCNI CTCAG 2 cut(s) 262, 301
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 2 cut(s) 76, 142
Bst4CI ACNGT 1 cut(s) 238
BstC8I GCNNGC 2 cut(s) 70, 278
BstDEI CTNAG 2 cut(s) 249, 288
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 145, 323
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 79, 145
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 2 cut(s) 76, 142
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 2 cut(s) 123, 224
BstV1I GCAGC 2 cut(s) 98, 210
BsuRI GGCC 2 cut(s) 44, 194
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 145, 323
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 105, 234, 328
Cac8I GCNNGC 2 cut(s) 70, 278
CfoI GCGC 1 cut(s) 223
CseI GACGC 1 cut(s) 192
CviAII CATG 3 cut(s) 80, 136, 163
CviJI RGCY 6 cut(s) 5, 44, 90, 194, 213, 280
CviKI_1 RGCY 6 cut(s) 5, 44, 90, 194, 213, 280
DdeI CTNAG 2 cut(s) 249, 288
DpnI GATC 2 cut(s) 78, 144
DpnII GATC 2 cut(s) 76, 142
EaeI YGGCCR 2 cut(s) 42, 192
Eco31I GGTCTC 1 cut(s) 97
Eco47III AGCGCT 1 cut(s) 222
FaeI CATG 3 cut(s) 83, 139, 166
FaiI YATR 5 cut(s) 81, 137, 164, 209, 216
FatI CATG 3 cut(s) 79, 135, 162
Fnu4HI GCNGC 4 cut(s) 45, 73, 112, 224
FokI GGATG 2 cut(s) 152, 330
Fsp4HI GCNGC 4 cut(s) 45, 73, 112, 224
GlaI GCGC 1 cut(s) 222
GluI GCNGC 4 cut(s) 45, 73, 112, 224
GsaI CCCAGC 1 cut(s) 94
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 2 cut(s) 44, 194
HapII CCGG 2 cut(s) 281, 340
HgaI GACGC 1 cut(s) 192
HhaI GCGC 1 cut(s) 223
Hin1II CATG 3 cut(s) 83, 139, 166
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 2 cut(s) 167, 292
HpaII CCGG 2 cut(s) 281, 340
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 4 cut(s) 172, 252, 291, 297
Hpy188III TCNNGA 2 cut(s) 146, 201
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4V TGCA 5 cut(s) 68, 135, 226, 276, 321
HpyF3I CTNAG 2 cut(s) 249, 288
Hsp92II CATG 3 cut(s) 83, 139, 166
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 2 cut(s) 76, 142
LpnPI CCDG 5 cut(s) 35, 54, 76, 111, 294
Lsp1109I GCAGC 2 cut(s) 98, 210
LweI GCATC 3 cut(s) 55, 195, 308
MalI GATC 2 cut(s) 78, 144
MbiI CCGCTC 1 cut(s) 47
MboI GATC 2 cut(s) 76, 142
MhlI GDGCHC 1 cut(s) 289
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 6 cut(s) 22, 109, 117, 208, 248, 274
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
Msp20I TGGCCA 1 cut(s) 194
MspI CCGG 2 cut(s) 281, 340
MspR9I CCNGG 1 cut(s) 282
NciI CCSGG 1 cut(s) 282
NdeII GATC 2 cut(s) 76, 142
NlaIII CATG 3 cut(s) 83, 139, 166
NlaIV GGNNCC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 4 cut(s) 46, 74, 113, 225
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 228
SatI GCNGC 4 cut(s) 45, 73, 112, 224
Sau3AI GATC 2 cut(s) 76, 142
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 289
SetI ASST 5 cut(s) 92, 109, 200, 215, 259
SfaNI GCATC 3 cut(s) 55, 195, 308
SfcI CTRYAG 2 cut(s) 123, 224
Sse9I AATT 1 cut(s) 7
SsiI CCGC 2 cut(s) 45, 72
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 1 cut(s) 238
TasI AATT 1 cut(s) 7
TauI GCSGC 2 cut(s) 47, 75
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 3 cut(s) 105, 241, 328
TseI GCWGC 2 cut(s) 111, 223
TspDTI ATGAA 1 cut(s) 231
TspGWI ACGGA 2 cut(s) 49, 330
TspRI CASTG 3 cut(s) 105, 241, 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.