MD11G1304400.v1.1

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010723
Physical Location & Seq
Forward (+)
41944242 .. 41945169
928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1304400.v1.1.491

Sequence Viewer

Length: 342 bp
ATGGCCAATTCCTCTGCCATTATCTTTTCCTTGGCCTTCGTTGCTGCTTTGATCATCTCCGTTGATGCAAGCGGCGGTCATGGGGCGAGCTGGGTTCCAGTGAGACCCCGCTGCGAGGGTTCAGTAGCGGAGTGCATGGATGATCATGATGATGTGTTTGGCATGGACTCGGAGATCAGCAGGCGCATCTTGGCCACCTCACAATACATAAGCTATGGAGCTCTGCAGAGAAACACTGTGCCTTGCTCTCAGAGAGGTGCTTCCTACTACAACTGCAAGCCGGGTGTTCAGAGCAACCCATACAACCGTGGATGCAGTGCTATTGCTCGTTGCCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

114

Amino Acids

12.11

Weight (kDa)

7.6

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 53 - 112 9e-29 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 72, 75, 109, 128
AcoI YGGCCR 2 cut(s) 3, 192
AfiI CCNNNNNNNGG 1 cut(s) 115
AluBI AGCT 3 cut(s) 90, 213, 221
AluI AGCT 3 cut(s) 90, 213, 221
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 3 cut(s) 3, 33, 192
ApeKI GCWGC 2 cut(s) 44, 111
AspLEI GCGC 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 282
BalI TGGCCA 2 cut(s) 5, 194
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 31, 98
BclI TGATCA 2 cut(s) 51, 142
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 1 cut(s) 224
BisI GCNGC 3 cut(s) 45, 73, 112
BlsI GCNGC 3 cut(s) 46, 74, 113
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 55, 195, 302
BpuMI CCSGG 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 30, 307
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 30, 307
BseGI GGATG 2 cut(s) 145, 317
BseLI CCNNNNNNNGG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 98
BseXI GCAGC 2 cut(s) 31, 98
BseYI CCCAGC 1 cut(s) 90
BshFI GGCC 3 cut(s) 5, 35, 194
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 2 cut(s) 281, 334
BslI CCNNNNNNNGG 1 cut(s) 115
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 3 cut(s) 5, 35, 194
Bso31I GGTCTC 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 223
Bsp143I GATC 3 cut(s) 51, 142, 174
BspACI CCGC 4 cut(s) 72, 75, 109, 128
BspANI GGCC 3 cut(s) 5, 35, 194
BspCNI CTCAG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 97
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 30, 307
BssMI GATC 3 cut(s) 51, 142, 174
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 238, 308
BstC8I GCNNGC 4 cut(s) 70, 88, 182, 278
BstDEI CTNAG 1 cut(s) 249
BstDSI CCRYGG 1 cut(s) 307
BstF5I GGATG 2 cut(s) 145, 317
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 3 cut(s) 54, 145, 177
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 3 cut(s) 51, 142, 174
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 31, 98
BsuRI GGCC 3 cut(s) 5, 35, 194
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 2 cut(s) 145, 317
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 3 cut(s) 105, 234, 322
Cac8I GCNNGC 4 cut(s) 70, 88, 182, 278
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 186
CviAII CATG 4 cut(s) 80, 136, 146, 163
CviJI RGCY 7 cut(s) 5, 35, 90, 194, 213, 221, 280
CviKI_1 RGCY 7 cut(s) 5, 35, 90, 194, 213, 221, 280
DdeI CTNAG 1 cut(s) 249
DpnI GATC 3 cut(s) 53, 144, 176
DpnII GATC 3 cut(s) 51, 142, 174
EaeI YGGCCR 2 cut(s) 3, 192
Ecl136II GAGCTC 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 223
Eco31I GGTCTC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 83, 139, 149, 166
FaiI YATR 7 cut(s) 81, 137, 147, 164, 209, 216, 301
FatI CATG 4 cut(s) 79, 135, 145, 162
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 2 cut(s) 51, 142
Fnu4HI GCNGC 3 cut(s) 45, 73, 112
FokI GGATG 2 cut(s) 152, 324
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 3 cut(s) 45, 73, 112
GlaI GCGC 1 cut(s) 185
GluI GCNGC 3 cut(s) 45, 73, 112
GsaI CCCAGC 1 cut(s) 94
HaeIII GGCC 3 cut(s) 5, 35, 194
HapII CCGG 2 cut(s) 281, 334
HhaI GCGC 1 cut(s) 186
Hin1II CATG 4 cut(s) 83, 139, 149, 166
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 1 cut(s) 167
HpaII CCGG 2 cut(s) 281, 334
Hpy188I TCNGA 3 cut(s) 172, 252, 291
Hpy188III TCNNGA 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 238, 308
HpyCH4V TGCA 5 cut(s) 68, 135, 226, 276, 315
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 1 cut(s) 249
Hsp92II CATG 4 cut(s) 83, 139, 149, 166
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 2 cut(s) 51, 142
Kzo9I GATC 3 cut(s) 51, 142, 174
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 4 cut(s) 76, 111, 166, 294
Lsp1109I GCAGC 2 cut(s) 31, 98
LweI GCATC 3 cut(s) 55, 195, 302
MalI GATC 3 cut(s) 53, 144, 176
MboI GATC 3 cut(s) 51, 142, 174
MhlI GDGCHC 1 cut(s) 223
MlsI TGGCCA 2 cut(s) 5, 194
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 2 cut(s) 5, 194
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 4 cut(s) 22, 109, 208, 248
Mox20I TGGCCA 2 cut(s) 5, 194
MscI TGGCCA 2 cut(s) 5, 194
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 2 cut(s) 5, 194
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 2 cut(s) 281, 334
MspR9I CCNGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 282
NdeII GATC 3 cut(s) 51, 142, 174
NlaIII CATG 4 cut(s) 83, 139, 149, 166
NlaIV GGNNCC 1 cut(s) 96
PagI TCATGA 1 cut(s) 145
PkrI GCNGC 3 cut(s) 46, 74, 113
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 1 cut(s) 223
SatI GCNGC 3 cut(s) 45, 73, 112
Sau3AI GATC 3 cut(s) 51, 142, 174
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 223
SetI ASST 5 cut(s) 92, 200, 215, 223, 259
SfaNI GCATC 3 cut(s) 55, 195, 302
SfcI CTRYAG 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 150
Sse9I AATT 1 cut(s) 7
SsiI CCGC 4 cut(s) 72, 75, 109, 128
SstI GAGCTC 1 cut(s) 223
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 238, 308
TasI AATT 1 cut(s) 7
TauI GCSGC 1 cut(s) 75
TscAI CASTG 3 cut(s) 105, 241, 322
TseI GCWGC 2 cut(s) 44, 111
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 3 cut(s) 105, 241, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.