RchiOBHm_Chr6g0293531

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010723
Physical Location & Seq
Forward (+)
56193904 .. 56194838
935 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26338

Sequence Viewer

Length: 399 bp
ATGGGTCTATTTCTGCTACTACTACTCGTTTTGATATTAAGGATTGCGAGGTCACGGATTCATCCAATTCCAGCTTTCCTTCCAGCAATTGGAGCAAGTTTGAATTTTGCCGTGATCATCGATGCAAGCAATGGCAGCGATCACGGGTTGAGCTTTGTTCCGGCCAAGGCCCGCTGCCAGGGTTCCATTGCAGAGTGCATGGCTGAAGATGAGTTTGAGATGGACTCTGAGATCAACAGGCGTATCTTGGCCACCACAAAGTACATCAGCTATGGAGCTCTGCAGAGGAACACTGTGCCATGCTCTCAGAGGGGTGCTTCCTACTACAACTGCAAGCCCGGCGCACAGGCAAACCCCTACAACCGTGGCTGCAGTGCCATCACTCGCTGCCGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

132

Amino Acids

14.38

Weight (kDa)

9.05

Isoelectric Point (pI)

44.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 71 - 131 6.4e-31 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 89
AciI CCGC 1 cut(s) 172
AcoI YGGCCR 2 cut(s) 162, 249
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 2 cut(s) 89, 178
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 4 cut(s) 74, 153, 270, 278
AluI AGCT 4 cut(s) 74, 153, 270, 278
Alw21I GWGCWC 1 cut(s) 280
AoxI GGCC 3 cut(s) 162, 168, 249
ApeKI GCWGC 4 cut(s) 135, 174, 369, 387
ApoI RAATTY 1 cut(s) 103
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 1 cut(s) 339
BaeI ACNNNNGTAYC 2 cut(s) 226, 259
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 280
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 4 cut(s) 147, 161, 356, 374
BccI CCATC 2 cut(s) 214, 386
BceAI ACGGC 2 cut(s) 95, 375
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 339
BfmI CTRYAG 2 cut(s) 281, 370
BisI GCNGC 4 cut(s) 136, 175, 370, 388
BlsI GCNGC 4 cut(s) 137, 176, 371, 389
Bme1390I CCNGG 2 cut(s) 179, 339
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 1 cut(s) 184
BmrFI CCNGG 2 cut(s) 179, 339
BmsI GCATC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpuMI CCSGG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 165, 178, 364
Bsc4I CCNNNNNNNGG 2 cut(s) 89, 178
Bse3DI GCAATG 2 cut(s) 136, 186
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 165, 178, 364
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 89, 178
BseMI GCAATG 2 cut(s) 136, 186
BseMII CTCAG 2 cut(s) 219, 320
BseXI GCAGC 4 cut(s) 147, 161, 356, 374
BshFI GGCC 3 cut(s) 164, 170, 251
BshVI ATCGAT 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 161, 339
BslI CCNNNNNNNGG 2 cut(s) 89, 178
BsnI GGCC 3 cut(s) 164, 170, 251
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 3 cut(s) 114, 139, 231
BspACI CCGC 1 cut(s) 172
BspANI GGCC 3 cut(s) 164, 170, 251
BspCNI CTCAG 2 cut(s) 220, 319
BspDI ATCGAT 1 cut(s) 120
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 2 cut(s) 285, 374
BsrDI GCAATG 2 cut(s) 136, 186
BssECI CCNNGG 3 cut(s) 165, 178, 364
BssMI GATC 3 cut(s) 114, 139, 231
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 2 cut(s) 295, 365
BstC8I GCNNGC 3 cut(s) 127, 172, 335
BstDEI CTNAG 2 cut(s) 228, 306
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 61
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 3 cut(s) 117, 142, 234
BstMBI GATC 3 cut(s) 114, 139, 231
BstMWI GCNNNNNNNGC 3 cut(s) 92, 135, 339
BstNI CCWGG 1 cut(s) 179
BstSCI CCNGG 2 cut(s) 177, 337
BstSFI CTRYAG 2 cut(s) 281, 370
BstV1I GCAGC 4 cut(s) 147, 161, 356, 374
Bsu15I ATCGAT 1 cut(s) 120
BsuRI GGCC 3 cut(s) 164, 170, 251
BsuTUI ATCGAT 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 379
BtsIMutI CAGTG 2 cut(s) 291, 379
Cac8I GCNNGC 3 cut(s) 127, 172, 335
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 169
ClaI ATCGAT 1 cut(s) 120
Csp6I GTAC 1 cut(s) 262
CviAII CATG 2 cut(s) 199, 300
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 2 cut(s) 228, 306
DpnI GATC 3 cut(s) 116, 141, 233
DpnII GATC 3 cut(s) 114, 139, 231
EaeI YGGCCR 2 cut(s) 162, 249
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 225
EcoICRI GAGCTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 202, 303
FaiI YATR 3 cut(s) 200, 273, 301
FatI CATG 2 cut(s) 198, 299
FauI CCCGC 1 cut(s) 179
FbaI TGATCA 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 136, 175, 370, 388
FokI GGATG 1 cut(s) 48
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 4 cut(s) 136, 175, 370, 388
GlaI GCGC 1 cut(s) 343
GluI GCNGC 4 cut(s) 136, 175, 370, 388
HaeIII GGCC 3 cut(s) 164, 170, 251
HapII CCGG 2 cut(s) 161, 339
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 202, 303
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HinfI GANTC 2 cut(s) 58, 224
HpaII CCGG 2 cut(s) 161, 339
Hpy188I TCNGA 2 cut(s) 229, 309
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 2 cut(s) 295, 365
HpyCH4V TGCA 6 cut(s) 125, 191, 198, 283, 333, 372
HpyF10VI GCNNNNNNNGC 3 cut(s) 92, 135, 339
HpyF3I CTNAG 2 cut(s) 228, 306
Hsp92II CATG 2 cut(s) 202, 303
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 3 cut(s) 114, 139, 231
LmnI GCTCC 2 cut(s) 92, 275
LpnPI CCDG 8 cut(s) 84, 96, 164, 174, 191, 223, 332, 352
Lsp1109I GCAGC 4 cut(s) 147, 161, 356, 374
LweI GCATC 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 51
MalI GATC 3 cut(s) 116, 141, 233
MboI GATC 3 cut(s) 114, 139, 231
MboII GAAGA 1 cut(s) 218
MfeI CAATTG 1 cut(s) 87
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 66, 87, 103
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 3 cut(s) 42, 279, 303
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 38
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 161, 339
MspR9I CCNGG 2 cut(s) 179, 339
MunI CAATTG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 3 cut(s) 92, 135, 339
NciI CCSGG 1 cut(s) 339
NdeII GATC 3 cut(s) 114, 139, 231
NlaIII CATG 2 cut(s) 202, 303
NlaIV GGNNCC 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 51
PfeI GAWTC 1 cut(s) 58
PflMI CCANNNNNTGG 1 cut(s) 89
PkrI GCNGC 4 cut(s) 137, 176, 371, 389
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 169
PstI CTGCAG 2 cut(s) 285, 374
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
SacI GAGCTC 1 cut(s) 280
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 4 cut(s) 136, 175, 370, 388
Sau3AI GATC 3 cut(s) 114, 139, 231
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 179, 339
SduI GDGCHC 1 cut(s) 280
SetI ASST 5 cut(s) 53, 76, 155, 272, 280
SfaNI GCATC 1 cut(s) 112
SfcI CTRYAG 2 cut(s) 281, 370
Sse9I AATT 3 cut(s) 66, 87, 103
SsiI CCGC 1 cut(s) 172
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 177, 337
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 2 cut(s) 295, 365
TaqI TCGA 1 cut(s) 120
TasI AATT 3 cut(s) 66, 87, 103
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 298, 379
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 4 cut(s) 135, 174, 369, 387
Tsp45I GTSAC 1 cut(s) 51
TspDTI ATGAA 1 cut(s) 50
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 2 cut(s) 298, 379
Van91I CCANNNNNTGG 1 cut(s) 89
XapI RAATTY 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.