MD16G1025200.v1.1

rapid alkalinization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010723
Physical Location & Seq
Reverse (-)
1811766 .. 1812113
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1025200.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCCAATTCCTCAGCGATTATCTTTTCCTTGGCCTTCGTGGCCGCTCTGATCATCTCCGTTGATGCAGGCGGCGATCATGGGGTGAGCTGGGTTCCAGTGAGACCTCGCCGCGAGGGTTCTGTAGCAGAGTGTATGGATGATCGTGATGATGAATTTGGCATGGACTCGGAGATTAGCAGGCGCATCTTGGCCACCTCACGAAACATAAGCTATGAAGCTCTGCAGAGAAACACTGTGCCTTGCTCTCAGAGAGGTGCTTCCTCCAACAACTGCAAGCCGGGTGCTCAGAATCAGAGTAATCCCTACAACCGTGGATGCAGTGCTAATGCTCGTTGCCGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

116

Amino Acids

12.41

Weight (kDa)

7.63

Isoelectric Point (pI)

55.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 114 5.3e-22 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 47
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 45, 72, 112
AcoI YGGCCR 3 cut(s) 3, 42, 192
AcsI RAATTY 1 cut(s) 155
AluBI AGCT 3 cut(s) 90, 213, 221
AluI AGCT 3 cut(s) 90, 213, 221
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 97
AoxI GGCC 4 cut(s) 3, 33, 42, 192
ApoI RAATTY 1 cut(s) 155
AspLEI GCGC 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 97
BalI TGGCCA 2 cut(s) 5, 194
Bbv12I GWGCWC 1 cut(s) 289
BbvCI CCTCAGC 1 cut(s) 13
BclI TGATCA 1 cut(s) 51
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 97
BfmI CTRYAG 2 cut(s) 123, 224
BglI GCCNNNNNGGC 1 cut(s) 41
BisI GCNGC 3 cut(s) 45, 73, 112
BlsI GCNGC 3 cut(s) 46, 74, 113
Bme1390I CCNGG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 55, 195, 308
Bpu10I CCTNAGC 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 2 cut(s) 30, 313
Bse1I ACTGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 30, 313
BseGI GGATG 2 cut(s) 145, 323
BseMII CTCAG 3 cut(s) 27, 263, 302
BseNI ACTGG 1 cut(s) 98
BseYI CCCAGC 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 4 cut(s) 5, 35, 44, 194
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 2 cut(s) 281, 340
BsmAI GTCTC 1 cut(s) 97
BsnI GGCC 4 cut(s) 5, 35, 44, 194
Bso31I GGTCTC 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 3 cut(s) 51, 76, 142
BspACI CCGC 3 cut(s) 45, 72, 112
BspANI GGCC 4 cut(s) 5, 35, 44, 194
BspCNI CTCAG 3 cut(s) 26, 262, 301
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 96
BspMAI CTGCAG 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 30, 313
BssMI GATC 3 cut(s) 51, 76, 142
BssT1I CCWWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 238, 314
BstC8I GCNNGC 3 cut(s) 70, 182, 278
BstDEI CTNAG 3 cut(s) 13, 249, 288
BstDSI CCRYGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 145, 323
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 3 cut(s) 54, 79, 145
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 3 cut(s) 51, 76, 142
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 280
BstSFI CTRYAG 2 cut(s) 123, 224
BstUI CGCG 1 cut(s) 114
BsuRI GGCC 4 cut(s) 5, 35, 44, 194
BtgI CCRYGG 1 cut(s) 313
BtsCI GGATG 2 cut(s) 145, 323
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 105, 234, 328
Cac8I GCNNGC 3 cut(s) 70, 182, 278
CfoI GCGC 1 cut(s) 186
CviAII CATG 2 cut(s) 80, 163
CviJI RGCY 8 cut(s) 5, 35, 44, 90, 194, 213, 221, 280
CviKI_1 RGCY 8 cut(s) 5, 35, 44, 90, 194, 213, 221, 280
DdeI CTNAG 3 cut(s) 13, 249, 288
DpnI GATC 3 cut(s) 53, 78, 144
DpnII GATC 3 cut(s) 51, 76, 142
EaeI YGGCCR 3 cut(s) 3, 42, 192
Eco130I CCWWGG 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 2 cut(s) 83, 166
FaiI YATR 5 cut(s) 81, 137, 164, 209, 216
FatI CATG 2 cut(s) 79, 162
FbaI TGATCA 1 cut(s) 51
Fnu4HI GCNGC 3 cut(s) 45, 73, 112
FokI GGATG 2 cut(s) 152, 330
Fsp4HI GCNGC 3 cut(s) 45, 73, 112
GlaI GCGC 1 cut(s) 185
GluI GCNGC 3 cut(s) 45, 73, 112
GsaI CCCAGC 1 cut(s) 94
HaeIII GGCC 4 cut(s) 5, 35, 44, 194
HapII CCGG 2 cut(s) 281, 340
HhaI GCGC 1 cut(s) 186
Hin1II CATG 2 cut(s) 83, 166
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HinfI GANTC 2 cut(s) 167, 292
HpaII CCGG 2 cut(s) 281, 340
HphI GGTGA 1 cut(s) 97
Hpy188I TCNGA 5 cut(s) 51, 172, 252, 291, 297
Hpy188III TCNNGA 2 cut(s) 146, 201
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 2 cut(s) 238, 314
HpyCH4V TGCA 4 cut(s) 68, 226, 276, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 3 cut(s) 13, 249, 288
Hsp92II CATG 2 cut(s) 83, 166
HspAI GCGC 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 3 cut(s) 51, 76, 142
LpnPI CCDG 5 cut(s) 54, 76, 111, 166, 294
LweI GCATC 3 cut(s) 55, 195, 308
MalI GATC 3 cut(s) 53, 78, 144
MbiI CCGCTC 1 cut(s) 47
MboI GATC 3 cut(s) 51, 76, 142
MhlI GDGCHC 1 cut(s) 289
MlsI TGGCCA 2 cut(s) 5, 194
MluCI AATT 2 cut(s) 7, 155
MluNI TGGCCA 2 cut(s) 5, 194
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 6 cut(s) 22, 109, 117, 208, 248, 274
Mox20I TGGCCA 2 cut(s) 5, 194
MscI TGGCCA 2 cut(s) 5, 194
Msp20I TGGCCA 2 cut(s) 5, 194
MspI CCGG 2 cut(s) 281, 340
MspR9I CCNGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 282
NdeII GATC 3 cut(s) 51, 76, 142
NlaIII CATG 2 cut(s) 83, 166
NlaIV GGNNCC 1 cut(s) 96
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 3 cut(s) 46, 74, 113
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 96
PstI CTGCAG 1 cut(s) 228
SatI GCNGC 3 cut(s) 45, 73, 112
Sau3AI GATC 3 cut(s) 51, 76, 142
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 289
SetI ASST 6 cut(s) 92, 109, 200, 215, 223, 259
SfaNI GCATC 3 cut(s) 55, 195, 308
SfcI CTRYAG 2 cut(s) 123, 224
SfiI GGCCNNNNNGGCC 1 cut(s) 41
Sse9I AATT 2 cut(s) 7, 155
SsiI CCGC 3 cut(s) 45, 72, 112
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 238, 314
TasI AATT 2 cut(s) 7, 155
TauI GCSGC 3 cut(s) 47, 75, 114
TfiI GAWTC 1 cut(s) 292
TscAI CASTG 3 cut(s) 105, 241, 328
TspDTI ATGAA 2 cut(s) 168, 231
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 3 cut(s) 105, 241, 328
XapI RAATTY 1 cut(s) 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.