FvH4_2g26340

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015551
Physical Location & Seq
Forward (+)
21178836 .. 21180842
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26340.t1

Sequence Viewer

Length: 1332 bp
ATGTCTACAACAATGGCCAAACCCAAAAGCCAAAGAAAACCCACCAAGCCAACCCACCAAAAGATCAAGAAACAGAATCAAAACCAGAACCAGAATCCCCAAAATAAGCCAGAGATCAAACCACCCTATTGGGCTGTTGTAAGAGGCTTACTGACTTGCAAACATCTCCAAGCAGTTCAGCTACTACAACAAGAAGGTAAGCATCACCCACAGAAACAACAAAGTGAGCAACTAGCAGCCATGGAGGAATCCAAGAAATGCAAGAAAATGAGATGCTCTGGCTCATTATGCAGCAACACAAAAATCACACACCGGCCTGAGCTAGGATCTCCTGAAATCCACAAGAAAAGAGCTTCTTCAATGGGCTGCTCCTCTAGCAGCACCAATGAAACTTCAAGCAGATCCATGAAGGCTCCTCTGCATGAGCTCAATGGAGTTGTCTCTACCTCTTCTTCTTCACTATCCGCATCTTCAAACATCTCATCTGCTGGTGGGTCATTCAGAGCAATGCCTTTCAGAAGATTCTCAGGTTGCTATGAGTGTAGAATGGTGGTTGACCCAGTTCTTGGTTTCACCAGAGACCCTTCTCTTAGGGGAAGCATTTGTTCATGTCCTGAATGTGGTGCCATTTTCATGAAAGCCGAAAACCTGGAGCTTCATCAAGCTGTTAGGCATGCAGTTTCCGAGCTTGGTCCAGAGGACACGAGCAAGAACATAGTGGAGATCATATTCCAATCCAGCTGGCTCAAGAAACAAGCCCCTATCTGCAAGATCGATCGGATCCTAAAAGTCCACAACACACAGAAAACCATCTCCAAATTCGAAGAGTACCGAGACTCCATCAAAGCCAAAGCCACCAAGCTGCAGCTCCCGAACAAGAAACACCCACGTTGCATTGCCGACGGCAACGAGCTTCTCAGGTTCCACTGCACCACCTTCGACTGCTCTTTGGGCCTCAACGGCTCCTCCAACCTCTGCAACTCCATCCCAAGCTGCAATGTCTGCAGCATTATCAAGAACGGCTTTAAAGTTGATCGGAGTGAGTTGGCCGGAAGTAGTGAGATTCTGACAACGGCGACAAGCGGGAGAGCGCATGACATGGCCGAGGTGGCGGCGGAGGACGGCCGGAGGGCGATGCTGGTGTGTAGAGTGATAGCTGGGAGAGTGAAGAAAAATGCGGATGGACAAGGTGGCGGTGGCGGTGGTGGTGGGGTGGAAGAGTACGACTCGGTGGCCGGAGCTGTGGGGGTGTATTCGAATTTGGATGAGTTGTATGTGTTTAATCCCAAGGCTATTTTGCCTTGTTTTGTTGTCATATATGGAGGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

48.32

Weight (kDa)

9.33

Isoelectric Point (pI)

52.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015551)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26340
malus_domestica MD08G1169800.v1.1 MD15G1356300.v1.1
prunus_persica Prupe.1G503600_v2.0.a1
pyrus_communis pycom08g14520 pycom15g31830
rosa_chinensis RchiOBHm_Chr6g0294921
rosa_laevigata RLG00000011827
rosa_multiflora Rmu_sc0002700.1_g000047
rosa_roxburghii Rroxscaffold_7G00172840
rosa_rugosa Rorug06G0248300
rosa_samantha Rh6AG361500 Rh6BG368900 Rh6CG375500 Rh6DG361900
rosa_wichuraiana Rw6G031520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 566
AccI GTMKAC 1 cut(s) 5
AciI CCGC 7 cut(s) 465, 1083, 1112, 1115, 1178, 1194, 1200
AclWI GGATC 4 cut(s) 334, 396, 775, 788
AcoI YGGCCR 5 cut(s) 15, 1047, 1101, 1123, 1233
AcsI RAATTY 2 cut(s) 818, 1258
AfaI GTAC 2 cut(s) 830, 1223
AfiI CCNNNNNNNGG 3 cut(s) 323, 566, 620
AgsI TTSAA 3 cut(s) 360, 396, 474
AjnI CCWGG 1 cut(s) 648
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 3 cut(s) 445, 573, 828
AlwI GGATC 4 cut(s) 334, 396, 775, 788
AoxI GGCC 7 cut(s) 15, 314, 952, 1047, 1101, 1123, 1233
ApeKI GCWGC 8 cut(s) 236, 291, 366, 378, 862, 865, 993, 1005
ApoI RAATTY 2 cut(s) 818, 1258
AspLEI GCGC 1 cut(s) 1093
AspS9I GGNCC 2 cut(s) 692, 952
AsuHPI GGTGA 2 cut(s) 197, 565
AsuII TTCGAA 2 cut(s) 822, 1256
AvaII GGWCC 1 cut(s) 692
BalI TGGCCA 1 cut(s) 17
BamHI GGATCC 1 cut(s) 780
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 429
BauI CACGAG 1 cut(s) 703
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 8 cut(s) 248, 303, 353, 390, 849, 877, 980, 1017
BccI CCATC 4 cut(s) 818, 848, 992, 1175
BceAI ACGGC 5 cut(s) 919, 976, 1036, 1089, 1138
BcgI CGANNNNNNTGC 2 cut(s) 919, 953
BciT130I CCWGG 1 cut(s) 650
BcoDI GTCTC 3 cut(s) 445, 573, 828
BfaI CTAG 3 cut(s) 233, 323, 375
BfmI CTRYAG 2 cut(s) 863, 1003
BglI GCCNNNNNGGC 2 cut(s) 960, 1109
BisI GCNGC 9 cut(s) 237, 292, 367, 379, 863, 866, 994, 1006, 1113
BlsI GCNGC 9 cut(s) 238, 293, 368, 380, 864, 867, 995, 1007, 1114
Bme1390I CCNGG 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 692
BmgT120I GGNCC 2 cut(s) 692, 952
BmiI GGNNCC 5 cut(s) 414, 625, 782, 923, 964
BmrFI CCNGG 1 cut(s) 650
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 4 cut(s) 211, 263, 476, 1125
BmuI ACTGGG 1 cut(s) 554
BplI GAGNNNNNCTC 2 cut(s) 1211, 1243
BpmI CTGGAG 1 cut(s) 671
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 2 cut(s) 822, 1256
BpuEI CTTGAG 1 cut(s) 731
Bsa29I ATCGAT 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 573
BsaJI CCNNGG 3 cut(s) 240, 1104, 1287
BsaXI ACNNNNNCTCC 4 cut(s) 821, 851, 950, 980
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 566, 620
Bse118I RCCGGY 1 cut(s) 312
Bse1I ACTGG 1 cut(s) 560
Bse3DI GCAATG 3 cut(s) 513, 894, 1003
BseBI CCWGG 1 cut(s) 650
BseCI ATCGAT 1 cut(s) 774
BseDI CCNNGG 3 cut(s) 240, 1104, 1287
BseGI GGATG 3 cut(s) 984, 1186, 1270
BseLI CCNNNNNNNGG 3 cut(s) 323, 566, 620
BseMI GCAATG 3 cut(s) 513, 894, 1003
BseMII CTCAG 3 cut(s) 309, 540, 931
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 3 cut(s) 361, 405, 955
BseX3I CGGCCG 1 cut(s) 1123
BseXI GCAGC 8 cut(s) 248, 303, 353, 390, 849, 877, 980, 1017
BseYI CCCAGC 1 cut(s) 1157
BsgI GTGCAG 1 cut(s) 913
Bsh1285I CGRYCG 2 cut(s) 778, 1126
BshFI GGCC 7 cut(s) 17, 316, 954, 1049, 1103, 1125, 1235
BshNI GGYRCC 1 cut(s) 623
BshVI ATCGAT 1 cut(s) 774
BsiEI CGRYCG 2 cut(s) 778, 1126
BsiHKAI GWGCWC 1 cut(s) 429
BsiSI CCGG 4 cut(s) 313, 1050, 1126, 1236
BslI CCNNNNNNNGG 3 cut(s) 323, 566, 620
BsmAI GTCTC 3 cut(s) 445, 573, 828
BsnI GGCC 7 cut(s) 17, 316, 954, 1049, 1103, 1125, 1235
Bso31I GGTCTC 1 cut(s) 573
Bsp119I TTCGAA 2 cut(s) 822, 1256
Bsp1286I GDGCHC 1 cut(s) 429
Bsp143I GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 7 cut(s) 465, 1083, 1112, 1115, 1178, 1194, 1200
BspANI GGCC 7 cut(s) 17, 316, 954, 1049, 1103, 1125, 1235
BspCNI CTCAG 3 cut(s) 310, 539, 930
BspDI ATCGAT 1 cut(s) 774
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 5 cut(s) 414, 625, 782, 923, 964
BspMAI CTGCAG 2 cut(s) 867, 1007
BspPI GGATC 4 cut(s) 334, 396, 775, 788
BspT104I TTCGAA 2 cut(s) 822, 1256
BspT107I GGYRCC 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 573
BsrDI GCAATG 3 cut(s) 513, 894, 1003
BsrFI RCCGGY 1 cut(s) 312
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 3 cut(s) 240, 1104, 1287
BssMI GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
BssSI CACGAG 1 cut(s) 703
BssT1I CCWWGG 2 cut(s) 240, 1287
Bst2BI CACGAG 1 cut(s) 703
Bst2UI CCWGG 1 cut(s) 650
Bst6I CTCTTC 3 cut(s) 454, 819, 1212
BstAPI GCANNNNNTGC 1 cut(s) 1002
BstBI TTCGAA 2 cut(s) 822, 1256
BstC8I GCNNGC 2 cut(s) 675, 743
BstDEI CTNAG 4 cut(s) 318, 526, 590, 917
BstDSI CCRYGG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 984, 1186, 1270
BstHHI GCGC 1 cut(s) 1093
BstKTI GATC 9 cut(s) 66, 117, 329, 404, 726, 774, 778, 783, 1036
BstMAI GTCTC 3 cut(s) 445, 573, 828
BstMBI GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
BstMCI CGRYCG 2 cut(s) 778, 1126
BstMWI GCNNNNNNNGC 6 cut(s) 288, 375, 951, 960, 1002, 1109
BstNI CCWGG 1 cut(s) 650
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 1 cut(s) 648
BstSFI CTRYAG 2 cut(s) 863, 1003
BstV1I GCAGC 8 cut(s) 248, 303, 353, 390, 849, 877, 980, 1017
BstX2I RGATCY 3 cut(s) 326, 401, 780
BstXI CCANNNNNNTGG 1 cut(s) 129
BstYI RGATCY 3 cut(s) 326, 401, 780
BstZI CGGCCG 1 cut(s) 1123
Bsu15I ATCGAT 1 cut(s) 774
BsuRI GGCC 7 cut(s) 17, 316, 954, 1049, 1103, 1125, 1235
BsuTUI ATCGAT 1 cut(s) 774
BtgI CCRYGG 1 cut(s) 240
BtgZI GCGATG 1 cut(s) 1148
BtsCI GGATG 3 cut(s) 984, 1186, 1270
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 1 cut(s) 925
Cac8I GCNNGC 2 cut(s) 675, 743
CciI TCATGA 1 cut(s) 633
CfoI GCGC 1 cut(s) 1093
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 2 cut(s) 692, 952
ClaI ATCGAT 1 cut(s) 774
Csp6I GTAC 2 cut(s) 829, 1222
CviAII CATG 8 cut(s) 241, 406, 422, 609, 634, 674, 1094, 1099
CviQI GTAC 2 cut(s) 829, 1222
DdeI CTNAG 4 cut(s) 318, 526, 590, 917
DpnI GATC 9 cut(s) 65, 116, 328, 403, 725, 773, 777, 782, 1035
DpnII GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
DraI TTTAAA 1 cut(s) 1027
EaeI YGGCCR 5 cut(s) 15, 1047, 1101, 1123, 1233
EagI CGGCCG 1 cut(s) 1123
Eam1104I CTCTTC 3 cut(s) 454, 819, 1212
EarI CTCTTC 3 cut(s) 454, 819, 1212
EciI GGCGGA 1 cut(s) 1130
Ecl136II GAGCTC 1 cut(s) 427
EclXI CGGCCG 1 cut(s) 1123
Eco130I CCWWGG 2 cut(s) 240, 1287
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 692
Eco52I CGGCCG 1 cut(s) 1123
Eco53kI GAGCTC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 240, 1287
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 2 cut(s) 240, 1287
FaeI CATG 8 cut(s) 244, 409, 425, 612, 637, 677, 1097, 1102
FalI AAGNNNNNCTT 4 cut(s) 340, 372, 1007, 1039
FatI CATG 8 cut(s) 240, 405, 421, 608, 633, 673, 1093, 1098
FauI CCCGC 1 cut(s) 1076
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 9 cut(s) 237, 292, 367, 379, 863, 866, 994, 1006, 1113
FokI GGATG 3 cut(s) 971, 1193, 1277
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 9 cut(s) 237, 292, 367, 379, 863, 866, 994, 1006, 1113
FspBI CTAG 3 cut(s) 233, 323, 375
GlaI GCGC 1 cut(s) 1092
GluI GCNGC 9 cut(s) 237, 292, 367, 379, 863, 866, 994, 1006, 1113
GsaI CCCAGC 1 cut(s) 1161
GsuI CTGGAG 1 cut(s) 671
HaeIII GGCC 7 cut(s) 17, 316, 954, 1049, 1103, 1125, 1235
HapII CCGG 4 cut(s) 313, 1050, 1126, 1236
HhaI GCGC 1 cut(s) 1093
Hin1II CATG 8 cut(s) 244, 409, 425, 612, 637, 677, 1097, 1102
Hin6I GCGC 1 cut(s) 1091
HinP1I GCGC 1 cut(s) 1091
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 7 cut(s) 76, 94, 248, 522, 836, 1063, 1226
HpaII CCGG 4 cut(s) 313, 1050, 1126, 1236
HphI GGTGA 2 cut(s) 197, 565
Hpy166II GTNNAC 3 cut(s) 6, 556, 793
Hpy188I TCNGA 6 cut(s) 503, 518, 685, 780, 1038, 1068
Hpy188III TCNNGA 8 cut(s) 67, 332, 614, 634, 695, 748, 871, 1015
Hpy8I GTNNAC 3 cut(s) 6, 556, 793
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 188, 403, 594, 946
HpyCH4IV ACGT 1 cut(s) 889
HpyF10VI GCNNNNNNNGC 6 cut(s) 288, 375, 951, 960, 1002, 1109
HpyF3I CTNAG 4 cut(s) 318, 526, 590, 917
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 8 cut(s) 244, 409, 425, 612, 637, 677, 1097, 1102
HspAI GCGC 1 cut(s) 1091
Kzo9I GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
LmnI GCTCC 6 cut(s) 374, 418, 652, 873, 968, 1238
Lsp1109I GCAGC 8 cut(s) 248, 303, 353, 390, 849, 877, 980, 1017
LweI GCATC 4 cut(s) 211, 263, 476, 1125
MaeI CTAG 3 cut(s) 233, 323, 375
MaeII ACGT 1 cut(s) 889
MalI GATC 9 cut(s) 65, 116, 328, 403, 725, 773, 777, 782, 1035
MboI GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
MboII GAAGA 9 cut(s) 348, 441, 444, 447, 462, 531, 836, 1180, 1229
MflI RGATCY 3 cut(s) 326, 401, 780
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 2 cut(s) 818, 1258
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 2 cut(s) 830, 1220
MmeI TCCRAC 1 cut(s) 993
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
MseI TTAA 2 cut(s) 1026, 1281
MslI CAYNNNNRTG 1 cut(s) 632
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 741
MspI CCGG 4 cut(s) 313, 1050, 1126, 1236
MspR9I CCNGG 1 cut(s) 650
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 6 cut(s) 288, 375, 951, 960, 1002, 1109
NcoI CCATGG 1 cut(s) 240
NdeII GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
NlaIII CATG 8 cut(s) 244, 409, 425, 612, 637, 677, 1097, 1102
NlaIV GGNNCC 5 cut(s) 414, 625, 782, 923, 964
NmeAIII GCCGAG 1 cut(s) 1129
NspI RCATGY 1 cut(s) 677
NspV TTCGAA 2 cut(s) 822, 1256
PaeI GCATGC 1 cut(s) 677
PagI TCATGA 1 cut(s) 633
PfeI GAWTC 5 cut(s) 76, 94, 248, 522, 1063
PflMI CCANNNNNTGG 1 cut(s) 566
PkrI GCNGC 9 cut(s) 238, 293, 368, 380, 864, 867, 995, 1007, 1114
Ple19I CGATCG 1 cut(s) 778
PleI GAGTC 2 cut(s) 830, 1220
PpsI GAGTC 2 cut(s) 830, 1220
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 1 cut(s) 648
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 5 cut(s) 414, 625, 782, 923, 964
PspPI GGNCC 2 cut(s) 692, 952
PstI CTGCAG 2 cut(s) 867, 1007
PsuI RGATCY 3 cut(s) 326, 401, 780
PvuI CGATCG 1 cut(s) 778
PvuII CAGCTG 1 cut(s) 741
RsaI GTAC 2 cut(s) 830, 1223
RsaNI GTAC 2 cut(s) 829, 1222
RseI CAYNNNNRTG 1 cut(s) 632
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 2 cut(s) 1026, 1281
SatI GCNGC 9 cut(s) 237, 292, 367, 379, 863, 866, 994, 1006, 1113
Sau3AI GATC 9 cut(s) 63, 114, 326, 401, 723, 771, 775, 780, 1033
Sau96I GGNCC 2 cut(s) 692, 952
SchI GAGTC 2 cut(s) 830, 1220
ScrFI CCNGG 1 cut(s) 650
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 4 cut(s) 211, 263, 476, 1125
SfcI CTRYAG 2 cut(s) 863, 1003
SfuI TTCGAA 2 cut(s) 822, 1256
SinI GGWCC 1 cut(s) 692
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
SphI GCATGC 1 cut(s) 677
Sse9I AATT 2 cut(s) 818, 1258
SsiI CCGC 7 cut(s) 465, 1083, 1112, 1115, 1178, 1194, 1200
SspMI CTAG 3 cut(s) 233, 323, 375
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 648
StyI CCWWGG 2 cut(s) 240, 1287
TaiI ACGT 1 cut(s) 892
TaqI TCGA 4 cut(s) 774, 822, 939, 1256
TasI AATT 2 cut(s) 818, 1258
TauI GCSGC 1 cut(s) 1115
TfiI GAWTC 5 cut(s) 76, 94, 248, 522, 1063
Tru1I TTAA 2 cut(s) 1026, 1281
Tru9I TTAA 2 cut(s) 1026, 1281
TscAI CASTG 1 cut(s) 932
TseI GCWGC 8 cut(s) 236, 291, 366, 378, 862, 865, 993, 1005
TspDTI ATGAA 6 cut(s) 402, 422, 597, 622, 647, 650
TspRI CASTG 1 cut(s) 932
Van91I CCANNNNNTGG 1 cut(s) 566
VpaK11BI GGWCC 1 cut(s) 692
XagI CCTNNNNNAGG 1 cut(s) 321
XapI RAATTY 2 cut(s) 818, 1258
XceI RCATGY 1 cut(s) 677
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 3 cut(s) 233, 323, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.