pycom08g14520

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015551
Physical Location & Seq
Reverse (-)
13761425 .. 13762515
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g14520.2

Sequence Viewer

Length: 777 bp
ATGTCTTCCTCAGCCATGGCTAGCTCTCATAAACCCAAAACCCAAAGAAAACCCACCAAGCAAACGCACCAAAAGCTCCAGAAGCACCATGAGAACAAGAACCCAAAGCCCCGAAAGGCAGAGGAGAAGCCGGCGGAGAAGCCGCCTTACTGGTCAGTTGTGAGAGGCTTACTGACCTGCAAACACCTCCAACCAGTTCAGCTACCACAAAAGCAACCTAATGAGCAACAAGCCATGGAGGAAGCTAGCAATGCTGCAAAGAAATGTAAGAAAATGAGATGTTCCGGCTCGTTGTGCAACAACACAAAAATAATGCAGAGGCCGGAACTGTTAGCATCTCCAGAAGTCCATAAGAAAAGGGCTTCTTCTTCAATGGGTTCTTCAAGCAACAGCAATGAGGCTTCAAGCAGATCCATGAAAGCTCCACTCCACGAAATCAACGGGGTTGTTTTGACATCTTCTTCTTCGTTTTCTGCAGTTTCTTCTTCCTCTAATAATAACTCTTCTGCTGGTGGTGGTGGGTCGTTTAGAGCAATGCCTTTTCGGCGATTTTCAGGCTGCTATGAGTGTAGGATGGTGGTTGATCCTGTTCTTGGTTTTGCTAGAGACCCTTCTCTGAGGGGCAGCATTTGTTCTTGCCCTGAATGTGGTGAGATTTTCACGAAAGCTGAGAACTTGGAGCTTCATCAGGCTGTTAGACATGCAGTTTCAGAACTTGGTCCTGAAGACACAAGCAAGAACATAGTGGATCGATCATATTCCAATCAAGCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.18

Weight (kDa)

9.62

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015551)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26340
malus_domestica MD08G1169800.v1.1 MD15G1356300.v1.1
prunus_persica Prupe.1G503600_v2.0.a1
pyrus_communis pycom08g14520 pycom15g31830
rosa_chinensis RchiOBHm_Chr6g0294921
rosa_laevigata RLG00000011827
rosa_multiflora Rmu_sc0002700.1_g000047
rosa_roxburghii Rroxscaffold_7G00172840
rosa_rugosa Rorug06G0248300
rosa_samantha Rh6AG361500 Rh6BG368900 Rh6CG375500 Rh6DG361900
rosa_wichuraiana Rw6G031520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
AciI CCGC 2 cut(s) 134, 143
AclWI GGATC 3 cut(s) 405, 578, 756
AcuI CTGAAG 1 cut(s) 744
AfiI CCNNNNNNNGG 2 cut(s) 593, 647
AgsI TTSAA 3 cut(s) 372, 384, 405
AluBI AGCT 8 cut(s) 24, 76, 202, 245, 422, 668, 682, 770
AluI AGCT 8 cut(s) 24, 76, 202, 245, 422, 668, 682, 770
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 3 cut(s) 405, 578, 756
AoxI GGCC 1 cut(s) 320
ApeKI GCWGC 3 cut(s) 254, 558, 624
AspS9I GGNCC 1 cut(s) 719
AsuHPI GGTGA 1 cut(s) 662
AsuNHI GCTAGC 2 cut(s) 20, 245
AvaII GGWCC 1 cut(s) 719
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BbsI GAAGAC 1 cut(s) 732
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 3 cut(s) 241, 545, 636
BccI CCATC 1 cut(s) 568
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 3 cut(s) 21, 246, 603
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 544
BisI GCNGC 4 cut(s) 143, 255, 559, 625
BlsI GCNGC 4 cut(s) 144, 256, 560, 626
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 1 cut(s) 719
BmsI GCATC 1 cut(s) 344
BmtI GCTAGC 2 cut(s) 24, 249
BpiI GAAGAC 1 cut(s) 732
BpmI CTGGAG 2 cut(s) 62, 324
Bpu10I CCTNAGC 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 751
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 2 cut(s) 15, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 647
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 155, 194
Bse3DI GCAATG 3 cut(s) 256, 400, 540
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 2 cut(s) 15, 234
BseGI GGATG 1 cut(s) 579
BseLI CCNNNNNNNGG 2 cut(s) 593, 647
BseMI GCAATG 3 cut(s) 256, 400, 540
BseMII CTCAG 3 cut(s) 24, 608, 660
BseNI ACTGG 2 cut(s) 155, 194
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 241, 545, 636
BshFI GGCC 1 cut(s) 322
BshVI ATCGAT 1 cut(s) 751
BsiSI CCGG 3 cut(s) 131, 285, 323
BslI CCNNNNNNNGG 2 cut(s) 593, 647
BsmAI GTCTC 1 cut(s) 600
BsnI GGCC 1 cut(s) 322
Bso31I GGTCTC 1 cut(s) 600
Bsp143I GATC 4 cut(s) 410, 583, 748, 752
Bsp19I CCATGG 2 cut(s) 15, 234
BspACI CCGC 2 cut(s) 134, 143
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 3 cut(s) 23, 609, 661
BspDI ATCGAT 1 cut(s) 751
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 2 cut(s) 24, 249
BspPI GGATC 3 cut(s) 405, 578, 756
BspTNI GGTCTC 1 cut(s) 600
BsrDI GCAATG 3 cut(s) 256, 400, 540
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 155, 194
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 2 cut(s) 15, 234
BssMI GATC 4 cut(s) 410, 583, 748, 752
BssT1I CCWWGG 2 cut(s) 15, 234
Bst4CI ACNGT 1 cut(s) 330
Bst6I CTCTTC 1 cut(s) 508
BstC8I GCNNGC 4 cut(s) 22, 132, 247, 772
BstDEI CTNAG 3 cut(s) 10, 617, 669
BstDSI CCRYGG 2 cut(s) 15, 234
BstF5I GGATG 1 cut(s) 579
BstKTI GATC 4 cut(s) 413, 586, 751, 755
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 4 cut(s) 410, 583, 748, 752
BstMWI GCNNNNNNNGC 5 cut(s) 73, 82, 251, 294, 544
BstNSI RCATGY 1 cut(s) 704
BstSFI CTRYAG 1 cut(s) 474
BstV1I GCAGC 3 cut(s) 241, 545, 636
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
Bsu15I ATCGAT 1 cut(s) 751
BsuRI GGCC 1 cut(s) 322
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 2 cut(s) 15, 234
BtsCI GGATG 1 cut(s) 579
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 4 cut(s) 22, 132, 247, 772
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 719
ClaI ATCGAT 1 cut(s) 751
CviAII CATG 5 cut(s) 16, 89, 235, 415, 701
DdeI CTNAG 3 cut(s) 10, 617, 669
DpnI GATC 4 cut(s) 412, 585, 750, 754
DpnII GATC 4 cut(s) 410, 583, 748, 752
Eam1104I CTCTTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 508
EciI GGCGGA 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 15, 234
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 719
Eco57I CTGAAG 1 cut(s) 744
EcoT14I CCWWGG 2 cut(s) 15, 234
ErhI CCWWGG 2 cut(s) 15, 234
FaeI CATG 5 cut(s) 19, 92, 238, 418, 704
FalI AAGNNNNNCTT 2 cut(s) 349, 381
FatI CATG 5 cut(s) 15, 88, 234, 414, 700
Fnu4HI GCNGC 4 cut(s) 143, 255, 559, 625
FokI GGATG 1 cut(s) 586
Fsp4HI GCNGC 4 cut(s) 143, 255, 559, 625
FspBI CTAG 3 cut(s) 21, 246, 603
GluI GCNGC 4 cut(s) 143, 255, 559, 625
GsuI CTGGAG 2 cut(s) 62, 324
HaeIII GGCC 1 cut(s) 322
HapII CCGG 3 cut(s) 131, 285, 323
Hin1II CATG 5 cut(s) 19, 92, 238, 418, 704
HpaII CCGG 3 cut(s) 131, 285, 323
HphI GGTGA 1 cut(s) 662
Hpy188I TCNGA 2 cut(s) 618, 712
Hpy188III TCNNGA 4 cut(s) 79, 341, 661, 722
HpyAV CCTTC 1 cut(s) 621
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4V TGCA 6 cut(s) 180, 257, 297, 316, 476, 704
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 82, 251, 294, 544
HpyF3I CTNAG 3 cut(s) 10, 617, 669
Hsp92II CATG 5 cut(s) 19, 92, 238, 418, 704
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 4 cut(s) 410, 583, 748, 752
LmnI GCTCC 3 cut(s) 81, 427, 679
Lsp1109I GCAGC 3 cut(s) 241, 545, 636
LweI GCATC 1 cut(s) 344
MaeI CTAG 3 cut(s) 21, 246, 603
MalI GATC 4 cut(s) 412, 585, 750, 754
MboI GATC 4 cut(s) 410, 583, 748, 752
MflI RGATCY 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 9 cut(s) 19, 115, 158, 197, 232, 312, 391, 499, 612
MroNI GCCGGC 1 cut(s) 130
MspI CCGG 3 cut(s) 131, 285, 323
MwoI GCNNNNNNNGC 5 cut(s) 73, 82, 251, 294, 544
NaeI GCCGGC 1 cut(s) 132
NcoI CCATGG 2 cut(s) 15, 234
NdeII GATC 4 cut(s) 410, 583, 748, 752
NgoMIV GCCGGC 1 cut(s) 130
NheI GCTAGC 2 cut(s) 20, 245
NlaIII CATG 5 cut(s) 19, 92, 238, 418, 704
NspI RCATGY 1 cut(s) 704
PdiI GCCGGC 1 cut(s) 132
PkrI GCNGC 4 cut(s) 144, 256, 560, 626
PspPI GGNCC 1 cut(s) 719
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 1 cut(s) 410
SatI GCNGC 4 cut(s) 143, 255, 559, 625
Sau3AI GATC 4 cut(s) 410, 583, 748, 752
Sau96I GGNCC 1 cut(s) 719
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 474
SinI GGWCC 1 cut(s) 719
SsiI CCGC 2 cut(s) 134, 143
SspMI CTAG 3 cut(s) 21, 246, 603
StyI CCWWGG 2 cut(s) 15, 234
TaaI ACNGT 1 cut(s) 330
TaqI TCGA 1 cut(s) 751
TauI GCSGC 1 cut(s) 145
TseI GCWGC 3 cut(s) 254, 558, 624
TspDTI ATGAA 2 cut(s) 431, 674
VpaK11BI GGWCC 1 cut(s) 719
XceI RCATGY 1 cut(s) 704
XspI CTAG 3 cut(s) 21, 246, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.