MD15G1356300.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015551
Physical Location & Seq
Reverse (-)
42963051 .. 42965101
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1356300.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGTCTTCCTCAGCCATGGCTAGCTCTAATAAACCAAAAACCCAAAGAAAACCCACCAAGCAAACACACCACAAGCTTCAAAAGCAACATGAGAACAAGAACCTGAAGCCCCAAAAGGCGGAGAAGAAGCCGGCAGAGAAGCCGCCTTACTGGTCAGTTGTGAGAGGCCTACTGACCTGCAAACACCTCCAACCGGTTCAGCTACCACAAAAGCAAAAGCAACCAAATGAGCAACAAGCCATGGAGGAAGCTGACAATGCAGCCAAGAAATGCAAGAAAATGAGATGTTCCGGCTCGTTGTGTAACAACACAAAAATAATGCAGAGGCCGGAAATGTTAGCATCTCCAGAAGTCCATAAGAAAAGGGCTTCTACTTCAATGGGTTCTTCAGGCCACAGCAATGAGACTTCAAGCAGATCCATGAAAGCTCCACTCCATGAAATCAATGGGGTTGTTTCCGCATCTTCTTCTTCGTTTTCTGCAGTTTCTTCTTCTAATAATTCTTCTTCTGCTGCTGGCGGTGGGTCGTTTAGAGCAATGCCGTTTCGGCGATTTTCGGGTTGTTATGAGTGCAGGATGGTGGTTGATCCTGTTCTTGGGTTTGCTAGAGACCCTTCTCTGAGGGGGAGCATCTGTCCTTGTCCGGAATGTGGTGAGATTTTCATGAAAGCTGAGAACTTGGAGCTTCATCAGGCTGTTAGACATGCAGTTTCTGAATTGGGTCCTGAAGACACTAGCAAGAACATAGTGGAGATTATATTCCAGTCCAGCTGGCTGAAAAAAGAGACCCCAATCTGCAAAATCGACCGCATCCTTAAAGTCCACAACACCCAAAATACCATCACCAAATTCGAAGAGTATAGAGACTCCATCAAAGCAAAAGCCACCAAGCTCCCCAAGAAACACCCCCGCTGCATCGCGGACGGCAACGAGCTCCTCCGTTTCCACTGCACCACCTTTGCCTGCTCCCTTGGCCTCAACGGCTCCTCCAACCTCTGCAACTCAATCCCACACTGCAATGTTTGCAGCATTATAAAAAACGGCTTCAACAAGTTCTCTGGGGAGTTGACGGAGCTGGGGATTTTGACAACCGCGACAAGCGGCAGAGCTCATGATAAGGCTGTGGTGGCGGCGGAGGAGGGTGACAAGAGGGCAATGCTGGTGTGCCGTGTGATTGCAGGGAGGGTGAAGAAGAACGTGGAGGGTAGTGTTGGAAATGGAAATGTTGGTATGGAAGAGTACGATTCTGTGGCCGGAGCTGTGGGAGTTTACTCGAATTTGGATGAGTTGTATGTGTTTAATCCCAAGGCTATTTTGCCTTGTTTTGTTGTGATATATGGAGGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

49.15

Weight (kDa)

9.33

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015551)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26340
malus_domestica MD08G1169800.v1.1 MD15G1356300.v1.1
prunus_persica Prupe.1G503600_v2.0.a1
pyrus_communis pycom08g14520 pycom15g31830
rosa_chinensis RchiOBHm_Chr6g0294921
rosa_laevigata RLG00000011827
rosa_multiflora Rmu_sc0002700.1_g000047
rosa_roxburghii Rroxscaffold_7G00172840
rosa_rugosa Rorug06G0248300
rosa_samantha Rh6AG361500 Rh6BG368900 Rh6CG375500 Rh6DG361900
rosa_wichuraiana Rw6G031520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1034
Acc36I ACCTGC 1 cut(s) 185
AccII CGCG 2 cut(s) 920, 1094
AccIII TCCGGA 1 cut(s) 643
AclWI GGATC 2 cut(s) 411, 581
AcoI YGGCCR 1 cut(s) 1251
AcsI RAATTY 2 cut(s) 848, 1276
AcuI CTGAAG 3 cut(s) 125, 372, 747
AfaI GTAC 1 cut(s) 1241
AfiI CCNNNNNNNGG 4 cut(s) 118, 193, 596, 650
AgeI ACCGGT 1 cut(s) 193
AgsI TTSAA 4 cut(s) 80, 378, 411, 1048
Alw21I GWGCWC 2 cut(s) 936, 1111
Alw26I GTCTC 4 cut(s) 398, 603, 779, 858
AlwI GGATC 2 cut(s) 411, 581
AlwNI CAGNNNCTG 1 cut(s) 713
Aor13HI TCCGGA 1 cut(s) 643
AoxI GGCC 5 cut(s) 166, 326, 391, 973, 1251
ApeKI GCWGC 4 cut(s) 260, 512, 912, 1026
ApoI RAATTY 2 cut(s) 848, 1276
AsiGI ACCGGT 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 722
AsuHPI GGTGA 4 cut(s) 665, 835, 1154, 1198
AsuII TTCGAA 1 cut(s) 852
AsuNHI GCTAGC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 722
BanII GRGCYC 2 cut(s) 936, 1111
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 2 cut(s) 936, 1111
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 272, 499, 899, 1038
BccI CCATC 3 cut(s) 571, 848, 878
BceAI ACGGC 5 cut(s) 526, 940, 997, 1057, 1152
BcoDI GTCTC 4 cut(s) 398, 603, 779, 858
BfaI CTAG 3 cut(s) 21, 606, 735
BfmI CTRYAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 185
BglI GCCNNNNNGGC 2 cut(s) 547, 981
BisI GCNGC 7 cut(s) 143, 261, 513, 913, 1027, 1102, 1131
BlsI GCNGC 7 cut(s) 144, 262, 514, 914, 1028, 1103, 1132
Bme18I GGWCC 1 cut(s) 722
BmgT120I GGNCC 1 cut(s) 722
BmiI GGNNCC 2 cut(s) 723, 985
BmsI GCATC 5 cut(s) 350, 470, 639, 819, 924
BmtI GCTAGC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 330
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 852
BsaI GGTCTC 2 cut(s) 603, 779
BsaJI CCNNGG 4 cut(s) 15, 240, 970, 1305
BsaWI WCCGGW 2 cut(s) 193, 643
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 971, 1001
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 193, 596, 650
Bse118I RCCGGY 2 cut(s) 130, 193
Bse1I ACTGG 2 cut(s) 155, 763
Bse3DI GCAATG 4 cut(s) 406, 543, 1024, 1161
BseAI TCCGGA 1 cut(s) 643
BseDI CCNNGG 4 cut(s) 15, 240, 970, 1305
BseGI GGATG 3 cut(s) 582, 810, 1288
BseLI CCNNNNNNNGG 4 cut(s) 118, 193, 596, 650
BseMI GCAATG 4 cut(s) 406, 543, 1024, 1161
BseMII CTCAG 3 cut(s) 24, 611, 663
BseNI ACTGG 2 cut(s) 155, 763
BseRI GAGGAG 3 cut(s) 926, 976, 1151
BseXI GCAGC 4 cut(s) 272, 499, 899, 1038
BseYI CCCAGC 1 cut(s) 1075
BsgI GTGCAG 2 cut(s) 592, 934
Bsh1236I CGCG 2 cut(s) 920, 1094
Bsh1285I CGRYCG 1 cut(s) 808
BshFI GGCC 5 cut(s) 168, 328, 393, 975, 1253
BshTI ACCGGT 1 cut(s) 193
BsiEI CGRYCG 1 cut(s) 808
BsiHKAI GWGCWC 2 cut(s) 936, 1111
BsiSI CCGG 6 cut(s) 131, 194, 291, 329, 644, 1254
BslI CCNNNNNNNGG 4 cut(s) 118, 193, 596, 650
BsmAI GTCTC 4 cut(s) 398, 603, 779, 858
BsnI GGCC 5 cut(s) 168, 328, 393, 975, 1253
Bso31I GGTCTC 2 cut(s) 603, 779
Bsp119I TTCGAA 1 cut(s) 852
Bsp1286I GDGCHC 2 cut(s) 936, 1111
Bsp13I TCCGGA 1 cut(s) 643
Bsp143I GATC 2 cut(s) 416, 586
Bsp19I CCATGG 2 cut(s) 15, 240
BspANI GGCC 5 cut(s) 168, 328, 393, 975, 1253
BspCNI CTCAG 3 cut(s) 23, 612, 664
BspEI TCCGGA 1 cut(s) 643
BspFNI CGCG 2 cut(s) 920, 1094
BspHI TCATGA 2 cut(s) 663, 1111
BspLI GGNNCC 2 cut(s) 723, 985
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 2 cut(s) 411, 581
BspT104I TTCGAA 1 cut(s) 852
BspTNI GGTCTC 2 cut(s) 603, 779
BsrDI GCAATG 4 cut(s) 406, 543, 1024, 1161
BsrFI RCCGGY 2 cut(s) 130, 193
BsrI ACTGG 2 cut(s) 155, 763
BssAI RCCGGY 2 cut(s) 130, 193
BssECI CCNNGG 4 cut(s) 15, 240, 970, 1305
BssMI GATC 2 cut(s) 416, 586
BssT1I CCWWGG 4 cut(s) 15, 240, 970, 1305
Bst6I CTCTTC 2 cut(s) 849, 1230
BstAPI GCANNNNNTGC 1 cut(s) 1023
BstBI TTCGAA 1 cut(s) 852
BstC8I GCNNGC 5 cut(s) 22, 132, 517, 773, 964
BstDEI CTNAG 3 cut(s) 10, 620, 672
BstDSI CCRYGG 2 cut(s) 15, 240
BstF5I GGATG 3 cut(s) 582, 810, 1288
BstFNI CGCG 2 cut(s) 920, 1094
BstKTI GATC 2 cut(s) 419, 589
BstMAI GTCTC 4 cut(s) 398, 603, 779, 858
BstMBI GATC 2 cut(s) 416, 586
BstMCI CGRYCG 1 cut(s) 808
BstMWI GCNNNNNNNGC 7 cut(s) 82, 257, 547, 972, 981, 1023, 1127
BstNSI RCATGY 1 cut(s) 707
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 2 cut(s) 920, 1094
BstV1I GCAGC 4 cut(s) 272, 499, 899, 1038
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 5 cut(s) 168, 328, 393, 975, 1253
BtgI CCRYGG 2 cut(s) 15, 240
BtgZI GCGATG 1 cut(s) 901
BtsCI GGATG 3 cut(s) 582, 810, 1288
BtsI GCAGTG 2 cut(s) 946, 1012
BtsIMutI CAGTG 2 cut(s) 946, 1012
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 5 cut(s) 22, 132, 517, 773, 964
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 2 cut(s) 663, 1111
Cfr10I RCCGGY 2 cut(s) 130, 193
Cfr13I GGNCC 1 cut(s) 722
Csp6I GTAC 1 cut(s) 1240
CspAI ACCGGT 1 cut(s) 193
CviAII CATG 8 cut(s) 16, 89, 241, 421, 437, 664, 704, 1112
CviQI GTAC 1 cut(s) 1240
DdeI CTNAG 3 cut(s) 10, 620, 672
DpnI GATC 2 cut(s) 418, 588
DpnII GATC 2 cut(s) 416, 586
EaeI YGGCCR 1 cut(s) 1251
Eam1104I CTCTTC 2 cut(s) 849, 1230
EarI CTCTTC 2 cut(s) 849, 1230
EciI GGCGGA 2 cut(s) 134, 1148
Ecl136II GAGCTC 2 cut(s) 934, 1109
Eco130I CCWWGG 4 cut(s) 15, 240, 970, 1305
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 2 cut(s) 936, 1111
Eco31I GGTCTC 2 cut(s) 603, 779
Eco47I GGWCC 1 cut(s) 722
Eco53kI GAGCTC 2 cut(s) 934, 1109
Eco57I CTGAAG 3 cut(s) 125, 372, 747
EcoICRI GAGCTC 2 cut(s) 934, 1109
EcoO109I RGGNCCY 1 cut(s) 722
EcoT14I CCWWGG 4 cut(s) 15, 240, 970, 1305
EcoT38I GRGCYC 2 cut(s) 936, 1111
ErhI CCWWGG 4 cut(s) 15, 240, 970, 1305
FaeI CATG 8 cut(s) 19, 92, 244, 424, 440, 667, 707, 1115
FatI CATG 8 cut(s) 15, 88, 240, 420, 436, 663, 703, 1111
FauI CCCGC 1 cut(s) 917
Fnu4HI GCNGC 7 cut(s) 143, 261, 513, 913, 1027, 1102, 1131
FokI GGATG 3 cut(s) 589, 797, 1295
FriOI GRGCYC 2 cut(s) 936, 1111
Fsp4HI GCNGC 7 cut(s) 143, 261, 513, 913, 1027, 1102, 1131
FspBI CTAG 3 cut(s) 21, 606, 735
GluI GCNGC 7 cut(s) 143, 261, 513, 913, 1027, 1102, 1131
GsaI CCCAGC 1 cut(s) 1079
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 5 cut(s) 168, 328, 393, 975, 1253
HapII CCGG 6 cut(s) 131, 194, 291, 329, 644, 1254
Hin1II CATG 8 cut(s) 19, 92, 244, 424, 440, 667, 707, 1115
HincII GTYRAC 1 cut(s) 1068
HindII GTYRAC 1 cut(s) 1068
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 2 cut(s) 866, 1244
HpaII CCGG 6 cut(s) 131, 194, 291, 329, 644, 1254
HphI GGTGA 4 cut(s) 665, 835, 1154, 1198
Hpy166II GTNNAC 3 cut(s) 823, 1068, 1270
Hpy188I TCNGA 2 cut(s) 621, 715
Hpy188III TCNNGA 5 cut(s) 347, 644, 664, 725, 1112
Hpy8I GTNNAC 3 cut(s) 823, 1068, 1270
HpyAV CCTTC 1 cut(s) 624
HpyCH4IV ACGT 1 cut(s) 1197
HpyF10VI GCNNNNNNNGC 7 cut(s) 82, 257, 547, 972, 981, 1023, 1127
HpyF3I CTNAG 3 cut(s) 10, 620, 672
HpySE526I ACGT 1 cut(s) 1197
Hsp92II CATG 8 cut(s) 19, 92, 244, 424, 440, 667, 707, 1115
Kpn2I TCCGGA 1 cut(s) 643
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 2 cut(s) 416, 586
LmnI GCTCC 9 cut(s) 433, 627, 682, 897, 939, 971, 989, 1072, 1256
Lsp1109I GCAGC 4 cut(s) 272, 499, 899, 1038
LweI GCATC 5 cut(s) 350, 470, 639, 819, 924
MaeI CTAG 3 cut(s) 21, 606, 735
MaeII ACGT 1 cut(s) 1197
MaeIII GTNAC 3 cut(s) 302, 1142, 1343
MalI GATC 2 cut(s) 418, 588
MboI GATC 2 cut(s) 416, 586
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 936, 1111
MluCI AATT 4 cut(s) 499, 716, 848, 1276
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 3 cut(s) 214, 1014, 1192
MroI TCCGGA 1 cut(s) 643
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 2 cut(s) 816, 1299
MslI CAYNNNNRTG 2 cut(s) 399, 1017
MspA1I CMGCKG 2 cut(s) 771, 912
MspI CCGG 6 cut(s) 131, 194, 291, 329, 644, 1254
MvnI CGCG 2 cut(s) 920, 1094
MwoI GCNNNNNNNGC 7 cut(s) 82, 257, 547, 972, 981, 1023, 1127
NaeI GCCGGC 1 cut(s) 132
NcoI CCATGG 2 cut(s) 15, 240
NdeII GATC 2 cut(s) 416, 586
NgoMIV GCCGGC 1 cut(s) 130
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 8 cut(s) 19, 92, 244, 424, 440, 667, 707, 1115
NlaIV GGNNCC 2 cut(s) 723, 985
NmuCI GTSAC 1 cut(s) 1142
NspI RCATGY 1 cut(s) 707
NspV TTCGAA 1 cut(s) 852
PagI TCATGA 2 cut(s) 663, 1111
PceI AGGCCT 1 cut(s) 168
PdiI GCCGGC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 1244
PinAI ACCGGT 1 cut(s) 193
PkrI GCNGC 7 cut(s) 144, 262, 514, 914, 1028, 1103, 1132
PleI GAGTC 1 cut(s) 860
PpsI GAGTC 1 cut(s) 860
PpuMI RGGWCCY 1 cut(s) 722
PsiI TTATAA 1 cut(s) 1034
Psp124BI GAGCTC 2 cut(s) 936, 1111
Psp5II RGGWCCY 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 1075
PspN4I GGNNCC 2 cut(s) 723, 985
PspPI GGNCC 1 cut(s) 722
PspPPI RGGWCCY 1 cut(s) 722
PstI CTGCAG 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 416
PvuII CAGCTG 1 cut(s) 771
RsaI GTAC 1 cut(s) 1241
RsaNI GTAC 1 cut(s) 1240
RseI CAYNNNNRTG 2 cut(s) 399, 1017
SacI GAGCTC 2 cut(s) 936, 1111
SaqAI TTAA 2 cut(s) 816, 1299
SatI GCNGC 7 cut(s) 143, 261, 513, 913, 1027, 1102, 1131
Sau3AI GATC 2 cut(s) 416, 586
Sau96I GGNCC 1 cut(s) 722
SchI GAGTC 1 cut(s) 860
SduI GDGCHC 2 cut(s) 936, 1111
SfaNI GCATC 5 cut(s) 350, 470, 639, 819, 924
SfcI CTRYAG 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 852
SinI GGWCC 1 cut(s) 722
SmiMI CAYNNNNRTG 2 cut(s) 399, 1017
Sse9I AATT 4 cut(s) 499, 716, 848, 1276
SseBI AGGCCT 1 cut(s) 168
SspMI CTAG 3 cut(s) 21, 606, 735
SstI GAGCTC 2 cut(s) 936, 1111
StuI AGGCCT 1 cut(s) 168
StyI CCWWGG 4 cut(s) 15, 240, 970, 1305
TaiI ACGT 1 cut(s) 1200
TaqI TCGA 3 cut(s) 804, 852, 1274
TasI AATT 4 cut(s) 499, 716, 848, 1276
TauI GCSGC 3 cut(s) 145, 1104, 1133
TfiI GAWTC 1 cut(s) 1244
Tru1I TTAA 2 cut(s) 816, 1299
Tru9I TTAA 2 cut(s) 816, 1299
TscAI CASTG 2 cut(s) 953, 1019
TseFI GTSAC 1 cut(s) 1142
TseI GCWGC 4 cut(s) 260, 512, 912, 1026
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 5 cut(s) 437, 453, 652, 677, 680
TspGWI ACGGA 2 cut(s) 929, 1085
TspRI CASTG 2 cut(s) 953, 1019
VpaK11BI GGWCC 1 cut(s) 722
XapI RAATTY 2 cut(s) 848, 1276
XceI RCATGY 1 cut(s) 707
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XspI CTAG 3 cut(s) 21, 606, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.