MD08G1169800.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0015551
Physical Location & Seq
Reverse (-)
20324842 .. 20326768
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1169800.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGTCTTCCTCAGCCATGGCTAGCTCTCATAAACCCAAAACCCAAAGAAAACCCACCAAGCAAACGCACCAAAAGCTCCAGAAGCACCATGAGAACAAGAACCCGAAGCCCCGAAAGGCAGAGGAGAAGCCGGCGGAGAAGCCGCCTTACTGGTCAGTTGTGAGAGGCTTACTGACCTGCAAACACCTCCAACCAGTTCAGCTACCACAAAAGCAACCTAATGAGCAACAAGCCATGGAGGAAGCTAACAATACTGCAAAGAAATGTAAGAAAATGAGATGTTCCGGCTCGTTGTGCAACAACACAAAAATAATGCAGAGGCCGGAACTGTTAGCATCTCCAGAAGTCCACAAGAAAAGGGCTTCTTCTTCAATGGGTTCTTCAAGCAACAGCAATGAGGCTTCAAGCAGATCCATGAAAGCTCCACTCCACGAAATCAACGGGGTTGTTTCGACATCTTCTTCTTCGTTTTCTGCAGTTTCTTCTTCCTCTAATAATAACTCTTCTGCTGGTGGTGGTGGGTCGTTTAGAGCAATGCCTTTTCGGCGATTTTCAGGCTGCTATGAGTGCAGGATGGTGGTTGATCCTGTTCTTGGTTTTGCTAGAGACCCTTCTCTGAGGGGCAGCATTTGTTCTTGCCCTGAATGTGGTGAGATTTTCATGAAAGCTGAGAACTTGGAGCTTCATCAGGCTGTTAGACATGCAGTTTCAGAACTTGGTCCTGAAGACACAAGCAAGAACATAGTGGAGATCATATTCCAATCAAGCTGGCTGAAAAAACAGACCCCAATCTGCAAAATCGACCGCATCCTTAAAGTCCACAACACCCAAAAAACCATCACCAAATTCGAAGAGTACAGAGACTCCATCAAAGCAAAAGCCACCAAGCTCCCCAAGAAACACCCCCGCTGCATCGCCGACGGCAACGAGCTCCTCCGTTTCCACTGCACCACATTTGCCTGCTCCCTCGGCCTCAACGGCTCCTCCAACCTCTGCAACTCAATCCCACACTGCAATGTATGCAGCATTATAAAAAACGGCTTCAACAAGTTCTCTGGGGAGCTGACGGAGCAGGGGATCTTGACGACCGCGACAAGCGGCAGAGCTCATGATAAGGCGGTGGTGGCGGCGGAGGAGGGTGACAAGAGGGCAATGCTGGTGTGCCGTGTGATTGCCGGGAGGGTGAAGAAGAACTTGGAGGGTAGTGTTGGAAATGGAAATGTTGGTATGGAAGAGTATGACTCTGTGGCTGGAGCTGTGGGAGTATACTCTAATTTGGATGAGTTGTATGTGTTCAATCCCAAGGCTATTTTGCCTTGTTTTGTTGTCATATATGGAGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

449

Amino Acids

49.07

Weight (kDa)

9.37

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015551)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26340
malus_domestica MD08G1169800.v1.1 MD15G1356300.v1.1
prunus_persica Prupe.1G503600_v2.0.a1
pyrus_communis pycom08g14520 pycom15g31830
rosa_chinensis RchiOBHm_Chr6g0294921
rosa_laevigata RLG00000011827
rosa_multiflora Rmu_sc0002700.1_g000047
rosa_roxburghii Rroxscaffold_7G00172840
rosa_rugosa Rorug06G0248300
rosa_samantha Rh6AG361500 Rh6BG368900 Rh6CG375500 Rh6DG361900
rosa_wichuraiana Rw6G031520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
Acc36I ACCTGC 1 cut(s) 185
AccI GTMKAC 1 cut(s) 1266
AccII CGCG 1 cut(s) 1091
AciI CCGC 9 cut(s) 134, 143, 805, 907, 1089, 1098, 1118, 1127, 1130
AclWI GGATC 3 cut(s) 405, 578, 1085
AcsI RAATTY 1 cut(s) 845
AcuI CTGAAG 1 cut(s) 744
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 2 cut(s) 593, 647
AgsI TTSAA 5 cut(s) 372, 384, 405, 1045, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1178, 1210
Alw21I GWGCWC 2 cut(s) 933, 1108
Alw26I GTCTC 2 cut(s) 600, 855
AlwI GGATC 3 cut(s) 405, 578, 1085
AoxI GGCC 2 cut(s) 320, 970
ApeKI GCWGC 4 cut(s) 558, 624, 909, 1023
ApoI RAATTY 1 cut(s) 845
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 1 cut(s) 1177
AsuHPI GGTGA 4 cut(s) 662, 832, 1151, 1195
AsuII TTCGAA 1 cut(s) 849
AsuNHI GCTAGC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 719
BanII GRGCYC 2 cut(s) 933, 1108
BarI GAAGNNNNNNTAC 2 cut(s) 131, 163
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 2 cut(s) 933, 1108
BbvCI CCTCAGC 1 cut(s) 10
BbvI GCAGC 4 cut(s) 545, 636, 896, 1035
BccI CCATC 3 cut(s) 568, 845, 875
BceAI ACGGC 4 cut(s) 937, 994, 1054, 1149
BcnI CCSGG 1 cut(s) 1177
BcoDI GTCTC 2 cut(s) 600, 855
BfaI CTAG 2 cut(s) 21, 603
BfmI CTRYAG 1 cut(s) 474
BfuAI ACCTGC 1 cut(s) 185
BglI GCCNNNNNGGC 2 cut(s) 544, 978
BisI GCNGC 7 cut(s) 143, 559, 625, 910, 1024, 1099, 1128
BlsI GCNGC 7 cut(s) 144, 560, 626, 911, 1025, 1100, 1129
Bme1390I CCNGG 1 cut(s) 1177
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 1 cut(s) 982
BmrFI CCNGG 1 cut(s) 1177
BmsI GCATC 3 cut(s) 344, 816, 921
BmtI GCTAGC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 1226, 1258
BpmI CTGGAG 3 cut(s) 62, 324, 1272
Bpu10I CCTNAGC 1 cut(s) 10
Bpu14I TTCGAA 1 cut(s) 849
BpuMI CCSGG 1 cut(s) 1177
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 15, 234, 967, 1302
BsaXI ACNNNNNCTCC 4 cut(s) 848, 878, 968, 998
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 647
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 2 cut(s) 155, 194
Bse3DI GCAATG 4 cut(s) 400, 540, 1021, 1158
BseDI CCNNGG 4 cut(s) 15, 234, 967, 1302
BseGI GGATG 3 cut(s) 579, 807, 1285
BseLI CCNNNNNNNGG 2 cut(s) 593, 647
BseMI GCAATG 4 cut(s) 400, 540, 1021, 1158
BseMII CTCAG 3 cut(s) 24, 608, 660
BseNI ACTGG 2 cut(s) 155, 194
BseRI GAGGAG 4 cut(s) 137, 923, 973, 1148
BseXI GCAGC 4 cut(s) 545, 636, 896, 1035
BsgI GTGCAG 2 cut(s) 589, 931
Bsh1236I CGCG 1 cut(s) 1091
Bsh1285I CGRYCG 2 cut(s) 805, 1089
BshFI GGCC 2 cut(s) 322, 972
BsiEI CGRYCG 2 cut(s) 805, 1089
BsiHKAI GWGCWC 2 cut(s) 933, 1108
BsiSI CCGG 4 cut(s) 131, 285, 323, 1176
BslI CCNNNNNNNGG 2 cut(s) 593, 647
BsmAI GTCTC 2 cut(s) 600, 855
BsnI GGCC 2 cut(s) 322, 972
Bso31I GGTCTC 1 cut(s) 600
Bsp119I TTCGAA 1 cut(s) 849
Bsp1286I GDGCHC 2 cut(s) 933, 1108
Bsp143I GATC 4 cut(s) 410, 583, 750, 1077
Bsp19I CCATGG 2 cut(s) 15, 234
BspACI CCGC 9 cut(s) 134, 143, 805, 907, 1089, 1098, 1118, 1127, 1130
BspANI GGCC 2 cut(s) 322, 972
BspCNI CTCAG 3 cut(s) 23, 609, 661
BspFNI CGCG 1 cut(s) 1091
BspHI TCATGA 2 cut(s) 660, 1108
BspLI GGNNCC 1 cut(s) 982
BspMAI CTGCAG 1 cut(s) 478
BspMI ACCTGC 1 cut(s) 185
BspOI GCTAGC 1 cut(s) 24
BspPI GGATC 3 cut(s) 405, 578, 1085
BspT104I TTCGAA 1 cut(s) 849
BspTNI GGTCTC 1 cut(s) 600
BsrDI GCAATG 4 cut(s) 400, 540, 1021, 1158
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 2 cut(s) 155, 194
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 4 cut(s) 15, 234, 967, 1302
BssMI GATC 4 cut(s) 410, 583, 750, 1077
BssNAI GTATAC 1 cut(s) 1267
BssT1I CCWWGG 3 cut(s) 15, 234, 1302
Bst1107I GTATAC 1 cut(s) 1267
Bst4CI ACNGT 1 cut(s) 330
Bst6I CTCTTC 3 cut(s) 508, 846, 1227
BstAPI GCANNNNNTGC 1 cut(s) 1020
BstBI TTCGAA 1 cut(s) 849
BstC8I GCNNGC 4 cut(s) 22, 132, 770, 961
BstDEI CTNAG 3 cut(s) 10, 617, 669
BstDSI CCRYGG 2 cut(s) 15, 234
BstF5I GGATG 3 cut(s) 579, 807, 1285
BstFNI CGCG 1 cut(s) 1091
BstKTI GATC 4 cut(s) 413, 586, 753, 1080
BstMAI GTCTC 2 cut(s) 600, 855
BstMBI GATC 4 cut(s) 410, 583, 750, 1077
BstMCI CGRYCG 2 cut(s) 805, 1089
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 1175
BstSFI CTRYAG 1 cut(s) 474
BstUI CGCG 1 cut(s) 1091
BstV1I GCAGC 4 cut(s) 545, 636, 896, 1035
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 2 cut(s) 410, 1077
BstYI RGATCY 2 cut(s) 410, 1077
BstZ17I GTATAC 1 cut(s) 1267
BsuRI GGCC 2 cut(s) 322, 972
BtgI CCRYGG 2 cut(s) 15, 234
BtgZI GCGATG 1 cut(s) 898
BtsCI GGATG 3 cut(s) 579, 807, 1285
BtsI GCAGTG 2 cut(s) 943, 1009
BtsIMutI CAGTG 2 cut(s) 943, 1009
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 4 cut(s) 22, 132, 770, 961
CciI TCATGA 2 cut(s) 660, 1108
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 719
Csp6I GTAC 1 cut(s) 856
CviAII CATG 7 cut(s) 16, 89, 235, 415, 661, 701, 1109
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 3 cut(s) 10, 617, 669
DpnI GATC 4 cut(s) 412, 585, 752, 1079
DpnII GATC 4 cut(s) 410, 583, 750, 1077
Eam1104I CTCTTC 3 cut(s) 508, 846, 1227
EarI CTCTTC 3 cut(s) 508, 846, 1227
EciI GGCGGA 2 cut(s) 149, 1145
Ecl136II GAGCTC 2 cut(s) 931, 1106
Eco130I CCWWGG 3 cut(s) 15, 234, 1302
Eco24I GRGCYC 2 cut(s) 933, 1108
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 1 cut(s) 719
Eco53kI GAGCTC 2 cut(s) 931, 1106
Eco57I CTGAAG 1 cut(s) 744
EcoICRI GAGCTC 2 cut(s) 931, 1106
EcoT14I CCWWGG 3 cut(s) 15, 234, 1302
EcoT38I GRGCYC 2 cut(s) 933, 1108
ErhI CCWWGG 3 cut(s) 15, 234, 1302
FaeI CATG 7 cut(s) 19, 92, 238, 418, 664, 704, 1112
FalI AAGNNNNNCTT 4 cut(s) 349, 381, 1178, 1210
FatI CATG 7 cut(s) 15, 88, 234, 414, 660, 700, 1108
FauI CCCGC 1 cut(s) 914
FblI GTMKAC 1 cut(s) 1266
Fnu4HI GCNGC 7 cut(s) 143, 559, 625, 910, 1024, 1099, 1128
FokI GGATG 3 cut(s) 586, 794, 1292
FriOI GRGCYC 2 cut(s) 933, 1108
Fsp4HI GCNGC 7 cut(s) 143, 559, 625, 910, 1024, 1099, 1128
FspBI CTAG 2 cut(s) 21, 603
GluI GCNGC 7 cut(s) 143, 559, 625, 910, 1024, 1099, 1128
GsuI CTGGAG 3 cut(s) 62, 324, 1272
HaeIII GGCC 2 cut(s) 322, 972
HapII CCGG 4 cut(s) 131, 285, 323, 1176
Hin1II CATG 7 cut(s) 19, 92, 238, 418, 664, 704, 1112
HinfI GANTC 2 cut(s) 863, 1241
HpaII CCGG 4 cut(s) 131, 285, 323, 1176
HphI GGTGA 4 cut(s) 662, 832, 1151, 1195
Hpy166II GTNNAC 3 cut(s) 349, 820, 1267
Hpy188I TCNGA 2 cut(s) 618, 712
Hpy188III TCNNGA 6 cut(s) 79, 341, 661, 722, 1081, 1109
Hpy8I GTNNAC 3 cut(s) 349, 820, 1267
Hpy99I CGWCG 1 cut(s) 923
HpyAV CCTTC 1 cut(s) 621
HpyCH4III ACNGT 1 cut(s) 330
HpyF3I CTNAG 3 cut(s) 10, 617, 669
Hsp92II CATG 7 cut(s) 19, 92, 238, 418, 664, 704, 1112
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Kzo9I GATC 4 cut(s) 410, 583, 750, 1077
Lsp1109I GCAGC 4 cut(s) 545, 636, 896, 1035
LweI GCATC 3 cut(s) 344, 816, 921
MaeI CTAG 2 cut(s) 21, 603
MaeIII GTNAC 1 cut(s) 1139
MalI GATC 4 cut(s) 412, 585, 752, 1079
MboI GATC 4 cut(s) 410, 583, 750, 1077
MflI RGATCY 2 cut(s) 410, 1077
MhlI GDGCHC 2 cut(s) 933, 1108
MluCI AATT 2 cut(s) 845, 1273
MlyI GAGTC 2 cut(s) 857, 1235
MmeI TCCRAC 3 cut(s) 214, 1011, 1189
MroNI GCCGGC 1 cut(s) 130
MseI TTAA 2 cut(s) 813, 1345
MslI CAYNNNNRTG 1 cut(s) 1014
MspA1I CMGCKG 1 cut(s) 909
MspI CCGG 4 cut(s) 131, 285, 323, 1176
MspR9I CCNGG 1 cut(s) 1177
MvnI CGCG 1 cut(s) 1091
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 1 cut(s) 1177
NcoI CCATGG 2 cut(s) 15, 234
NdeII GATC 4 cut(s) 410, 583, 750, 1077
NgoMIV GCCGGC 1 cut(s) 130
NheI GCTAGC 1 cut(s) 20
NlaIII CATG 7 cut(s) 19, 92, 238, 418, 664, 704, 1112
NlaIV GGNNCC 1 cut(s) 982
NmeAIII GCCGAG 1 cut(s) 948
NmuCI GTSAC 1 cut(s) 1139
NspI RCATGY 1 cut(s) 704
NspV TTCGAA 1 cut(s) 849
PagI TCATGA 2 cut(s) 660, 1108
PdiI GCCGGC 1 cut(s) 132
PkrI GCNGC 7 cut(s) 144, 560, 626, 911, 1025, 1100, 1129
PleI GAGTC 2 cut(s) 857, 1235
PpsI GAGTC 2 cut(s) 857, 1235
PsiI TTATAA 1 cut(s) 1031
Psp124BI GAGCTC 2 cut(s) 933, 1108
PspN4I GGNNCC 1 cut(s) 982
PspPI GGNCC 1 cut(s) 719
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 2 cut(s) 410, 1077
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
RseI CAYNNNNRTG 1 cut(s) 1014
SacI GAGCTC 2 cut(s) 933, 1108
SaqAI TTAA 2 cut(s) 813, 1345
SatI GCNGC 7 cut(s) 143, 559, 625, 910, 1024, 1099, 1128
Sau3AI GATC 4 cut(s) 410, 583, 750, 1077
Sau96I GGNCC 1 cut(s) 719
SchI GAGTC 2 cut(s) 857, 1235
ScrFI CCNGG 1 cut(s) 1177
SduI GDGCHC 2 cut(s) 933, 1108
SfaNI GCATC 3 cut(s) 344, 816, 921
SfcI CTRYAG 1 cut(s) 474
SfuI TTCGAA 1 cut(s) 849
SinI GGWCC 1 cut(s) 719
SmiMI CAYNNNNRTG 1 cut(s) 1014
Sse9I AATT 2 cut(s) 845, 1273
SsiI CCGC 9 cut(s) 134, 143, 805, 907, 1089, 1098, 1118, 1127, 1130
SspMI CTAG 2 cut(s) 21, 603
SstI GAGCTC 2 cut(s) 933, 1108
StyD4I CCNGG 1 cut(s) 1175
StyI CCWWGG 3 cut(s) 15, 234, 1302
TaaI ACNGT 1 cut(s) 330
TaqI TCGA 3 cut(s) 452, 801, 849
TasI AATT 2 cut(s) 845, 1273
TatI WGTACW 1 cut(s) 855
TauI GCSGC 3 cut(s) 145, 1101, 1130
Tru1I TTAA 2 cut(s) 813, 1345
Tru9I TTAA 2 cut(s) 813, 1345
TscAI CASTG 2 cut(s) 950, 1016
TseFI GTSAC 1 cut(s) 1139
TseI GCWGC 4 cut(s) 558, 624, 909, 1023
Tsp45I GTSAC 1 cut(s) 1139
TspDTI ATGAA 4 cut(s) 431, 649, 674, 677
TspGWI ACGGA 2 cut(s) 926, 1082
TspRI CASTG 2 cut(s) 950, 1016
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 1 cut(s) 845
XceI RCATGY 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 1266
XspI CTAG 2 cut(s) 21, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.