FvH4_2g28010

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006780
Physical Location & Seq
Forward (+)
22106538 .. 22107402
865 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28010.t1

Sequence Viewer

Length: 480 bp
ATGTCTCCCCTGATCAGCAACGATCTAGAGCGGATTTTCGAGAAGCTTGACAAGAACCGAGACGGGCAAGTGAGTCTCGAGGAGCTCAACTGGCTCCTCGAGAGAATCGGAGTCGAGTTCAGCCTGCATGAGCTCGAGTCTCTTGTTGGCAAACCGGGGCTCGACTTCAATGAGTTCTTGTTCTTCTACAAGTCCATATCTGCGCCGGATAGTAATGACGGCGACGATGGTAGTGGTAGTGGCAGTAGTGATCATGAAGACGAGAGTGATCTTGTGAAGGCGTTCAATGTGTTCGATGTAAACGGCGACGGTTACATTTCTTGTGAGGAGCTGGACAGCGTTTTGAGGAGATTAGGAGTAATGGAAGAGAATAGTAGCCGTGACTGCAGAAGTATGATTCACGTGTACGACACCAATTTGGACGGGATGCTTGATTTCCAGGAATTCAAACACATGATGTTGCAGAATACCATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

160

Amino Acids

18.04

Weight (kDa)

4.24

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 8 - 34 6e-06 EF hand domain
EF-hand_7 PF13499 88 - 153 2.1e-14 EF-hand domain pair
EF-hand_1 PF00036 91 - 118 3.8e-07 EF hand domain
EF-hand_6 PF13405 91 - 119 1.1e-07 EF-hand domain
EF-hand_5 PF13202 92 - 114 3.3e-06 EF hand
EF-hand_1 PF00036 128 - 153 6.6e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 31
AciI CCGC 1 cut(s) 31
AcsI RAATTY 1 cut(s) 443
AcvI CACGTG 1 cut(s) 403
AfaI GTAC 1 cut(s) 407
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 169, 286, 448
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 4 cut(s) 46, 85, 133, 331
AluI AGCT 4 cut(s) 46, 85, 133, 331
Alw21I GWGCWC 2 cut(s) 87, 135
Alw26I GTCTC 4 cut(s) 9, 54, 80, 144
Ama87I CYCGRG 3 cut(s) 77, 98, 134
ApoI RAATTY 1 cut(s) 443
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 1 cut(s) 205
AsuC2I CCSGG 1 cut(s) 156
AvaI CYCGRG 3 cut(s) 77, 98, 134
BanII GRGCYC 3 cut(s) 87, 135, 162
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 2 cut(s) 87, 135
BccI CCATC 1 cut(s) 221
BceAI ACGGC 3 cut(s) 235, 319, 363
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 2 cut(s) 12, 250
BcnI CCSGG 1 cut(s) 156
BcoDI GTCTC 4 cut(s) 9, 54, 80, 144
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 385
Bme1390I CCNGG 2 cut(s) 156, 440
BmeT110I CYCGRG 3 cut(s) 77, 98, 134
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 2 cut(s) 156, 440
BmsI GCATC 1 cut(s) 417
BpiI GAAGAC 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 156
BsaAI YACGTR 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 4 cut(s) 86, 95, 341, 361
BsiHKAI GWGCWC 2 cut(s) 87, 135
BsiHKCI CYCGRG 3 cut(s) 77, 98, 134
BsiSI CCGG 2 cut(s) 155, 206
BsmAI GTCTC 4 cut(s) 9, 54, 80, 144
BsmBI CGTCTC 1 cut(s) 54
BsoBI CYCGRG 3 cut(s) 77, 98, 134
Bsp1286I GDGCHC 3 cut(s) 87, 135, 162
Bsp143I GATC 4 cut(s) 12, 22, 250, 268
BspACI CCGC 1 cut(s) 31
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 95
BspMAI CTGCAG 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 31
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 4 cut(s) 12, 22, 250, 268
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 360
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 1 cut(s) 125
BstF5I GGATG 1 cut(s) 432
BstHHI GCGC 1 cut(s) 205
BstKTI GATC 4 cut(s) 15, 25, 253, 271
BstMAI GTCTC 4 cut(s) 9, 54, 80, 144
BstMBI GATC 4 cut(s) 12, 22, 250, 268
BstMWI GCNNNNNNNGC 2 cut(s) 91, 384
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 154, 438
BstSFI CTRYAG 1 cut(s) 385
BstV2I GAAGAC 1 cut(s) 264
BtsCI GGATG 1 cut(s) 432
Cac8I GCNNGC 1 cut(s) 125
CciI TCATGA 1 cut(s) 253
CfoI GCGC 1 cut(s) 205
Csp6I GTAC 1 cut(s) 406
CviAII CATG 3 cut(s) 128, 254, 454
CviJI RGCY 8 cut(s) 46, 85, 94, 123, 133, 160, 331, 378
CviKI_1 RGCY 8 cut(s) 46, 85, 94, 123, 133, 160, 331, 378
CviQI GTAC 1 cut(s) 406
DpnI GATC 4 cut(s) 14, 24, 252, 270
DpnII GATC 4 cut(s) 12, 22, 250, 268
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
Ecl136II GAGCTC 2 cut(s) 85, 133
Eco24I GRGCYC 3 cut(s) 87, 135, 162
Eco53kI GAGCTC 2 cut(s) 85, 133
Eco72I CACGTG 1 cut(s) 403
Eco88I CYCGRG 3 cut(s) 77, 98, 134
EcoICRI GAGCTC 2 cut(s) 85, 133
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 1 cut(s) 438
EcoT38I GRGCYC 3 cut(s) 87, 135, 162
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 3 cut(s) 131, 257, 457
FaiI YATR 5 cut(s) 129, 197, 255, 395, 455
FatI CATG 3 cut(s) 127, 253, 453
FbaI TGATCA 2 cut(s) 12, 250
FokI GGATG 1 cut(s) 439
FriOI GRGCYC 3 cut(s) 87, 135, 162
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 204
HapII CCGG 2 cut(s) 155, 206
HhaI GCGC 1 cut(s) 205
Hin1II CATG 3 cut(s) 131, 257, 457
Hin6I GCGC 1 cut(s) 203
HinP1I GCGC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 5 cut(s) 73, 105, 111, 137, 397
HpaII CCGG 2 cut(s) 155, 206
Hpy166II GTNNAC 2 cut(s) 301, 406
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 5 cut(s) 26, 40, 77, 100, 254
Hpy8I GTNNAC 2 cut(s) 301, 406
Hpy99I CGWCG 2 cut(s) 227, 311
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 3 cut(s) 127, 387, 463
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 384
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 3 cut(s) 131, 257, 457
HspAI GCGC 1 cut(s) 203
Ksp22I TGATCA 2 cut(s) 12, 250
Kzo9I GATC 4 cut(s) 12, 22, 250, 268
LmnI GCTCC 3 cut(s) 82, 99, 328
LpnPI CCDG 8 cut(s) 23, 76, 137, 168, 219, 317, 425, 452
LweI GCATC 1 cut(s) 417
MaeI CTAG 1 cut(s) 26
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 2 cut(s) 311, 380
MalI GATC 4 cut(s) 14, 24, 252, 270
MbiI CCGCTC 1 cut(s) 31
MboI GATC 4 cut(s) 12, 22, 250, 268
MboII GAAGA 3 cut(s) 175, 269, 377
MhlI GDGCHC 3 cut(s) 87, 135, 162
MluCI AATT 2 cut(s) 415, 443
MlyI GAGTC 3 cut(s) 82, 120, 146
MnlI CCTC 4 cut(s) 73, 107, 319, 339
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 1 cut(s) 478
MspI CCGG 2 cut(s) 155, 206
MspR9I CCNGG 2 cut(s) 156, 440
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 91, 384
NciI CCSGG 1 cut(s) 156
NdeII GATC 4 cut(s) 12, 22, 250, 268
NlaIII CATG 3 cut(s) 131, 257, 457
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 380
PaeR7I CTCGAG 3 cut(s) 77, 98, 134
PagI TCATGA 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 105, 397
PfoI TCCNGGA 1 cut(s) 438
PleI GAGTC 3 cut(s) 81, 119, 145
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 3 cut(s) 81, 119, 145
Ppu21I YACGTR 1 cut(s) 403
Psp124BI GAGCTC 2 cut(s) 87, 135
Psp6I CCWGG 1 cut(s) 438
PspCI CACGTG 1 cut(s) 403
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 1 cut(s) 95
PspXI VCTCGAGB 1 cut(s) 134
PstI CTGCAG 1 cut(s) 389
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SacI GAGCTC 2 cut(s) 87, 135
SaqAI TTAA 1 cut(s) 478
Sau3AI GATC 4 cut(s) 12, 22, 250, 268
SchI GAGTC 3 cut(s) 82, 120, 146
ScrFI CCNGG 2 cut(s) 156, 440
SduI GDGCHC 3 cut(s) 87, 135, 162
SetI ASST 5 cut(s) 48, 87, 135, 333, 405
SfaNI GCATC 1 cut(s) 417
SfcI CTRYAG 1 cut(s) 385
Sfr274I CTCGAG 3 cut(s) 77, 98, 134
SlaI CTCGAG 3 cut(s) 77, 98, 134
SmlI CTYRAG 3 cut(s) 77, 98, 134
SmoI CTYRAG 3 cut(s) 77, 98, 134
Sse9I AATT 2 cut(s) 415, 443
SsiI CCGC 1 cut(s) 31
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 2 cut(s) 87, 135
StyD4I CCNGG 2 cut(s) 154, 438
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 1 cut(s) 405
TaqI TCGA 7 cut(s) 39, 78, 99, 114, 135, 162, 294
TasI AATT 2 cut(s) 415, 443
TfiI GAWTC 2 cut(s) 105, 397
Tru1I TTAA 1 cut(s) 478
Tru9I TTAA 1 cut(s) 478
TseFI GTSAC 1 cut(s) 380
Tsp45I GTSAC 1 cut(s) 380
TspDTI ATGAA 1 cut(s) 270
XapI RAATTY 1 cut(s) 443
XbaI TCTAGA 1 cut(s) 25
XhoI CTCGAG 3 cut(s) 77, 98, 134
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.