MD15G1133000.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006780
Physical Location & Seq
Reverse (-)
9647701 .. 9648222
522 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133000.v1.1.491

Sequence Viewer

Length: 522 bp
ATGACAACAATCTACATGATGTGTCCTCTAAGCACCAACGACTTGCACCGTATTTTCGAAAATCTCGACAAGAATGGAGACGGGCAAGTGAGTCTCGAGGAGCTCAATTGGCTCCTCGAGAGAATCGGCGTGCAATTCAGCCTGCACGAGCTCGAGTCCCTCTTGGGAAAACCGAGCCTTGACATCAACGAGTTCTTGTTCTTCTACAAGTCCATATCCATGCAGCAAGGTGACGCCAGTGGCGAAAATGAAGGCGGTGACATTCGTGAGGATGTCGGTGTTGATGGTCAGGAAGATGAAGATGAGAAGGACCTTGCGAAGGCATTCGGTGTGTTTGACTTGAACGGCGATGGCTTCATTTCTAGCGAGGAGCTTGAGAGCGTGCTGAGGAGATTAGGGGTTTTGGAGGAGAATAGCAGCCGGGACTGTAGAACCATGATTCGCGTGTTCGACACCAACTTGGACGGCCTGCTCGACTTTCAGGAATTCAAAACCATGATGTTCCAAAATACTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

174

Amino Acids

19.65

Weight (kDa)

4.17

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 102 - 167 2.8e-13 EF-hand domain pair
EF-hand_1 PF00036 105 - 132 1.4e-06 EF hand domain
EF-hand_6 PF13405 105 - 133 1.1e-06 EF-hand domain
EF-hand_5 PF13202 106 - 128 1.4e-07 EF hand
EF-hand_8 PF13833 117 - 169 8.8e-08 EF-hand domain pair
EF-hand_1 PF00036 143 - 167 2.8e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 444
AciI CCGC 1 cut(s) 255
AcsI RAATTY 1 cut(s) 485
AcyI GRCGYC 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 2 cut(s) 343, 490
AluBI AGCT 3 cut(s) 103, 151, 373
AluI AGCT 3 cut(s) 103, 151, 373
Alw21I GWGCWC 2 cut(s) 105, 153
Alw26I GTCTC 2 cut(s) 72, 98
Ama87I CYCGRG 3 cut(s) 95, 116, 152
AoxI GGCC 1 cut(s) 466
ApeKI GCWGC 2 cut(s) 223, 417
ApoI RAATTY 1 cut(s) 485
Asp700I GAANNNNTTC 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 310
AsuC2I CCSGG 1 cut(s) 422
AsuHPI GGTGA 2 cut(s) 242, 269
AsuII TTCGAA 1 cut(s) 57
AvaI CYCGRG 3 cut(s) 95, 116, 152
AvaII GGWCC 1 cut(s) 310
BanII GRGCYC 2 cut(s) 105, 153
BauI CACGAG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 105, 153
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 2 cut(s) 235, 429
BccI CCATC 2 cut(s) 278, 344
BceAI ACGGC 2 cut(s) 361, 481
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 72, 98
BfaI CTAG 1 cut(s) 363
BfmI CTRYAG 1 cut(s) 427
BisI GCNGC 2 cut(s) 224, 418
BlsI GCNGC 2 cut(s) 225, 419
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 3 cut(s) 95, 116, 152
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 1 cut(s) 113
BmrFI CCNGG 1 cut(s) 422
Bpu10I CCTNAGC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 234
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMII CTCAG 1 cut(s) 377
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 5 cut(s) 104, 113, 383, 403, 422
BseXI GCAGC 2 cut(s) 235, 429
BsgI GTGCAG 1 cut(s) 128
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 1 cut(s) 468
BsiHKAI GWGCWC 2 cut(s) 105, 153
BsiHKCI CYCGRG 3 cut(s) 95, 116, 152
BsiSI CCGG 1 cut(s) 421
BslFI GGGAC 2 cut(s) 142, 437
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 72, 98
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 142, 437
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 1 cut(s) 468
BsoBI CYCGRG 3 cut(s) 95, 116, 152
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 105, 153
BspACI CCGC 1 cut(s) 255
BspANI GGCC 1 cut(s) 468
BspCNI CTCAG 1 cut(s) 378
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 1 cut(s) 113
BspT104I TTCGAA 1 cut(s) 57
BsrI ACTGG 1 cut(s) 237
BssNI GRCGYC 1 cut(s) 234
BssSI CACGAG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 50, 428
BstACI GRCGYC 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 131, 143, 383, 470
BstDEI CTNAG 2 cut(s) 29, 386
BstENI CCTNNNNNAGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 277
BstFNI CGCG 1 cut(s) 444
BstMAI GTCTC 2 cut(s) 72, 98
BstMWI GCNNNNNNNGC 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 427
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 235, 429
BsuRI GGCC 1 cut(s) 468
BtgZI GCGATG 1 cut(s) 363
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 244
Cac8I GCNNGC 4 cut(s) 131, 143, 383, 470
Cfr13I GGNCC 1 cut(s) 310
CseI GACGC 1 cut(s) 242
CviAII CATG 4 cut(s) 16, 220, 436, 496
CviJI RGCY 9 cut(s) 103, 112, 141, 151, 177, 354, 373, 420, 468
CviKI_1 RGCY 9 cut(s) 103, 112, 141, 151, 177, 354, 373, 420, 468
DdeI CTNAG 2 cut(s) 29, 386
Ecl136II GAGCTC 2 cut(s) 103, 151
Eco24I GRGCYC 2 cut(s) 105, 153
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 2 cut(s) 103, 151
Eco88I CYCGRG 3 cut(s) 95, 116, 152
EcoICRI GAGCTC 2 cut(s) 103, 151
EcoNI CCTNNNNNAGG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 485
EcoT38I GRGCYC 2 cut(s) 105, 153
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 4 cut(s) 19, 223, 439, 499
FaiI YATR 5 cut(s) 17, 215, 221, 437, 497
FaqI GGGAC 2 cut(s) 142, 437
FatI CATG 4 cut(s) 15, 219, 435, 495
Fnu4HI GCNGC 2 cut(s) 224, 418
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 2 cut(s) 105, 153
Fsp4HI GCNGC 2 cut(s) 224, 418
FspBI CTAG 1 cut(s) 363
GluI GCNGC 2 cut(s) 224, 418
HaeIII GGCC 1 cut(s) 468
HapII CCGG 1 cut(s) 421
HgaI GACGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 4 cut(s) 19, 223, 439, 499
HinfI GANTC 4 cut(s) 91, 123, 155, 439
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 242, 269
Hpy188III TCNNGA 6 cut(s) 65, 95, 118, 266, 290, 482
HpyAV CCTTC 3 cut(s) 245, 301, 313
HpyCH4III ACNGT 2 cut(s) 50, 428
HpyCH4V TGCA 4 cut(s) 46, 133, 145, 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 109
HpyF3I CTNAG 2 cut(s) 29, 386
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 4 cut(s) 19, 223, 439, 499
LmnI GCTCC 3 cut(s) 100, 117, 370
LpnPI CCDG 6 cut(s) 155, 250, 275, 434, 467, 482
Lsp1109I GCAGC 2 cut(s) 235, 429
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 230, 257
MboII GAAGA 3 cut(s) 193, 305, 311
MfeI CAATTG 1 cut(s) 106
MhlI GDGCHC 2 cut(s) 105, 153
MluCI AATT 3 cut(s) 106, 134, 485
MlyI GAGTC 2 cut(s) 100, 164
MnlI CCTC 8 cut(s) 36, 91, 125, 170, 262, 361, 381, 400
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 1 cut(s) 520
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 1 cut(s) 421
MspR9I CCNGG 1 cut(s) 422
MunI CAATTG 1 cut(s) 106
Mva1269I GAATGC 1 cut(s) 323
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 109
NciI CCSGG 1 cut(s) 422
NlaIII CATG 4 cut(s) 19, 223, 439, 499
NlaIV GGNNCC 1 cut(s) 113
NmuCI GTSAC 2 cut(s) 230, 257
NspV TTCGAA 1 cut(s) 57
PaeR7I CTCGAG 3 cut(s) 95, 116, 152
PcsI WCGNNNNNNNCGW 3 cut(s) 63, 240, 471
PctI GAATGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 2 cut(s) 123, 439
PkrI GCNGC 2 cut(s) 225, 419
PleI GAGTC 2 cut(s) 99, 163
PpsI GAGTC 2 cut(s) 99, 163
PpuMI RGGWCCY 1 cut(s) 310
Psp124BI GAGCTC 2 cut(s) 105, 153
Psp5II RGGWCCY 1 cut(s) 310
PspN4I GGNNCC 1 cut(s) 113
PspPI GGNCC 1 cut(s) 310
PspPPI RGGWCCY 1 cut(s) 310
PspXI VCTCGAGB 1 cut(s) 152
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 2 cut(s) 105, 153
SaqAI TTAA 1 cut(s) 520
SatI GCNGC 2 cut(s) 224, 418
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 2 cut(s) 100, 164
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 2 cut(s) 105, 153
SetI ASST 5 cut(s) 105, 153, 232, 315, 375
SfcI CTRYAG 1 cut(s) 427
Sfr274I CTCGAG 3 cut(s) 95, 116, 152
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 3 cut(s) 95, 116, 152
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 4 cut(s) 95, 116, 152, 374
SmoI CTYRAG 4 cut(s) 95, 116, 152, 374
Sse9I AATT 3 cut(s) 106, 134, 485
SsiI CCGC 1 cut(s) 255
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 2 cut(s) 105, 153
StyD4I CCNGG 1 cut(s) 420
TaaI ACNGT 2 cut(s) 50, 428
TaqI TCGA 7 cut(s) 57, 66, 96, 117, 153, 450, 474
TasI AATT 3 cut(s) 106, 134, 485
TfiI GAWTC 2 cut(s) 123, 439
Tru1I TTAA 1 cut(s) 520
Tru9I TTAA 1 cut(s) 520
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 230, 257
TseI GCWGC 2 cut(s) 223, 417
Tsp45I GTSAC 2 cut(s) 230, 257
TspDTI ATGAA 3 cut(s) 264, 312, 346
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 310
XagI CCTNNNNNAGG 1 cut(s) 317
XapI RAATTY 1 cut(s) 485
XhoI CTCGAG 3 cut(s) 95, 116, 152
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.