pycom15g11990

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006780
Physical Location & Seq
Reverse (-)
8145322 .. 8145834
513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g11990.1

Sequence Viewer

Length: 513 bp
ATGATGTGTCCTCTAAGCACCAACGACTTGCACCGTATTTTCGAAAATCTCGACAAGAATGGAGACGGGCAAGTGAGTCTCGAGGAGCTCAATTGGCTCATCGAGAGAATCGGCGTGCAATTCAGCTTGCACGAGCTCGAGTCCCTCGTGGGAAAACCGAGCCTTGACATCAACGAGTTCGTGTTCTTCTACAAGTCCATATCCATGCAGCAAGGTGACGCCGGTGGCGAAAATGAAGGCGGTGACATTCGTGAGGATGTCGGTGTTGATGGTCAGGAAGATGAAGATGAAGATGAGAAGGACCTTGCGAAGGCATTTGGTGTGTTTGACTTGAACGGCGATGGCTTCATTTCCAGCGAGGAGCTTGAGAGCGTGCTGAGGCGATTGGGGGTTTTGGAGGAGAATAGCAGCCGGGACTGTAGAACTATGATTCGCGTGTTCGACACCAACTTGGACGGCCTGCTCGACTTTCAGGAATTCAAAACCATGATGTTCCAAAATACTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

171

Amino Acids

19.23

Weight (kDa)

4.12

Isoelectric Point (pI)

33.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 8 - 52 8.8e-06 EF-hand domain pair
EF-hand_7 PF13499 99 - 164 2.7e-13 EF-hand domain pair
EF-hand_1 PF00036 102 - 129 1.4e-06 EF hand domain
EF-hand_6 PF13405 102 - 130 1e-06 EF-hand domain
EF-hand_5 PF13202 103 - 125 1.4e-07 EF hand
EF-hand_8 PF13833 114 - 166 8.7e-08 EF-hand domain pair
EF-hand_1 PF00036 140 - 164 2.8e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 435
AciI CCGC 1 cut(s) 240
AcsI RAATTY 1 cut(s) 476
AcyI GRCGYC 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 2 cut(s) 334, 481
AluBI AGCT 4 cut(s) 88, 126, 136, 364
AluI AGCT 4 cut(s) 88, 126, 136, 364
Alw21I GWGCWC 2 cut(s) 90, 138
Alw26I GTCTC 2 cut(s) 57, 83
Ama87I CYCGRG 2 cut(s) 80, 137
AoxI GGCC 1 cut(s) 457
ApeKI GCWGC 2 cut(s) 208, 408
ApoI RAATTY 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 301
AsuC2I CCSGG 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 227, 254
AsuII TTCGAA 1 cut(s) 42
AvaI CYCGRG 2 cut(s) 80, 137
AvaII GGWCC 1 cut(s) 301
BanII GRGCYC 2 cut(s) 90, 138
BauI CACGAG 2 cut(s) 131, 146
Bbv12I GWGCWC 2 cut(s) 90, 138
BbvCI CCTCAGC 1 cut(s) 377
BbvI GCAGC 2 cut(s) 220, 420
BccI CCATC 2 cut(s) 263, 335
BceAI ACGGC 2 cut(s) 352, 472
BcnI CCSGG 1 cut(s) 413
BcoDI GTCTC 2 cut(s) 57, 83
BfmI CTRYAG 1 cut(s) 418
BisI GCNGC 2 cut(s) 209, 409
BlsI GCNGC 2 cut(s) 210, 410
Bme1390I CCNGG 1 cut(s) 413
Bme18I GGWCC 1 cut(s) 301
BmeT110I CYCGRG 2 cut(s) 80, 137
BmgT120I GGNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 413
Bpu10I CCTNAGC 1 cut(s) 377
Bpu14I TTCGAA 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 221
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 368
BseRI GAGGAG 3 cut(s) 98, 374, 413
BseXI GCAGC 2 cut(s) 220, 420
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 1 cut(s) 459
BsiHKAI GWGCWC 2 cut(s) 90, 138
BsiHKCI CYCGRG 2 cut(s) 80, 137
BsiSI CCGG 2 cut(s) 222, 412
BslFI GGGAC 2 cut(s) 127, 428
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 2 cut(s) 57, 83
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 127, 428
BsnI GGCC 1 cut(s) 459
BsoBI CYCGRG 2 cut(s) 80, 137
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 90, 138
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 459
BspCNI CTCAG 1 cut(s) 369
BspFNI CGCG 1 cut(s) 435
BspT104I TTCGAA 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 221
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 2 cut(s) 131, 146
Bst2BI CACGAG 2 cut(s) 131, 146
Bst4CI ACNGT 2 cut(s) 35, 419
BstACI GRCGYC 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 4 cut(s) 116, 128, 374, 461
BstDEI CTNAG 2 cut(s) 14, 377
BstENI CCTNNNNNAGG 1 cut(s) 308
BstF5I GGATG 1 cut(s) 262
BstFNI CGCG 1 cut(s) 435
BstMAI GTCTC 2 cut(s) 57, 83
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstSCI CCNGG 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 418
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 220, 420
BsuRI GGCC 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 262
Cac8I GCNNGC 4 cut(s) 116, 128, 374, 461
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 1 cut(s) 227
CviAII CATG 2 cut(s) 205, 487
CviJI RGCY 9 cut(s) 88, 97, 126, 136, 162, 345, 364, 411, 459
CviKI_1 RGCY 9 cut(s) 88, 97, 126, 136, 162, 345, 364, 411, 459
DdeI CTNAG 2 cut(s) 14, 377
Ecl136II GAGCTC 2 cut(s) 88, 136
Eco24I GRGCYC 2 cut(s) 90, 138
Eco47I GGWCC 1 cut(s) 301
Eco53kI GAGCTC 2 cut(s) 88, 136
Eco88I CYCGRG 2 cut(s) 80, 137
EcoICRI GAGCTC 2 cut(s) 88, 136
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 476
EcoT38I GRGCYC 2 cut(s) 90, 138
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 208, 490
FaiI YATR 4 cut(s) 200, 206, 428, 488
FaqI GGGAC 2 cut(s) 127, 428
FatI CATG 2 cut(s) 204, 486
Fnu4HI GCNGC 2 cut(s) 209, 409
FokI GGATG 1 cut(s) 269
FriOI GRGCYC 2 cut(s) 90, 138
Fsp4HI GCNGC 2 cut(s) 209, 409
GluI GCNGC 2 cut(s) 209, 409
HaeIII GGCC 1 cut(s) 459
HapII CCGG 2 cut(s) 222, 412
HgaI GACGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 2 cut(s) 208, 490
HinfI GANTC 4 cut(s) 76, 108, 140, 430
HpaII CCGG 2 cut(s) 222, 412
HphI GGTGA 2 cut(s) 227, 254
Hpy188III TCNNGA 6 cut(s) 50, 80, 103, 251, 275, 473
HpyAV CCTTC 3 cut(s) 230, 292, 304
HpyCH4III ACNGT 2 cut(s) 35, 419
HpyCH4V TGCA 4 cut(s) 31, 118, 130, 208
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 2 cut(s) 14, 377
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 2 cut(s) 208, 490
LmnI GCTCC 2 cut(s) 85, 361
LpnPI CCDG 6 cut(s) 235, 260, 367, 425, 458, 473
Lsp1109I GCAGC 2 cut(s) 220, 420
MaeIII GTNAC 2 cut(s) 215, 242
MboII GAAGA 4 cut(s) 178, 290, 296, 302
MfeI CAATTG 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 90, 138
MluCI AATT 3 cut(s) 91, 119, 476
MlyI GAGTC 2 cut(s) 85, 149
MnlI CCTC 7 cut(s) 21, 76, 155, 247, 352, 372, 391
MseI TTAA 1 cut(s) 511
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 2 cut(s) 222, 412
MspR9I CCNGG 1 cut(s) 413
MunI CAATTG 1 cut(s) 91
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 94
NciI CCSGG 1 cut(s) 413
NlaIII CATG 2 cut(s) 208, 490
NmuCI GTSAC 2 cut(s) 215, 242
NspV TTCGAA 1 cut(s) 42
PaeR7I CTCGAG 2 cut(s) 80, 137
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 144, 225, 462
PfeI GAWTC 2 cut(s) 108, 430
PkrI GCNGC 2 cut(s) 210, 410
PleI GAGTC 2 cut(s) 84, 148
PpsI GAGTC 2 cut(s) 84, 148
PpuMI RGGWCCY 1 cut(s) 301
Psp124BI GAGCTC 2 cut(s) 90, 138
Psp5II RGGWCCY 1 cut(s) 301
PspPI GGNCC 1 cut(s) 301
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 2 cut(s) 90, 138
SaqAI TTAA 1 cut(s) 511
SatI GCNGC 2 cut(s) 209, 409
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 85, 149
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 90, 138
SetI ASST 6 cut(s) 90, 128, 138, 217, 306, 366
SfcI CTRYAG 1 cut(s) 418
Sfr274I CTCGAG 2 cut(s) 80, 137
SfuI TTCGAA 1 cut(s) 42
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 1 cut(s) 301
SlaI CTCGAG 2 cut(s) 80, 137
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 3 cut(s) 80, 137, 365
SmoI CTYRAG 3 cut(s) 80, 137, 365
Sse9I AATT 3 cut(s) 91, 119, 476
SsiI CCGC 1 cut(s) 240
SstI GAGCTC 2 cut(s) 90, 138
StyD4I CCNGG 1 cut(s) 411
TaaI ACNGT 2 cut(s) 35, 419
TaqI TCGA 7 cut(s) 42, 51, 81, 102, 138, 441, 465
TasI AATT 3 cut(s) 91, 119, 476
TfiI GAWTC 2 cut(s) 108, 430
Tru1I TTAA 1 cut(s) 511
Tru9I TTAA 1 cut(s) 511
TseFI GTSAC 2 cut(s) 215, 242
TseI GCWGC 2 cut(s) 208, 408
Tsp45I GTSAC 2 cut(s) 215, 242
TspDTI ATGAA 4 cut(s) 249, 297, 303, 337
VpaK11BI GGWCC 1 cut(s) 301
XagI CCTNNNNNAGG 1 cut(s) 308
XapI RAATTY 1 cut(s) 476
XhoI CTCGAG 2 cut(s) 80, 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.