Rorug06G0267100

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006780
Physical Location & Seq
Reverse (-)
41343064 .. 41344443
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0267100.1

Sequence Viewer

Length: 858 bp
ATGAGTATGGAGATAGATACCTACCAGAAAGAAAGTGATGATCAGTATGTTGATTGGAAAGGAAGAAAGGCAAATCCTAAAATCCATGGTGGAATTAGAGCTGCTGCCTTTGCATGTGTTGTTGAGGTCTTTACCAACATGGTTTTCTTATGCAATGCCAGTAACTTTGTCACATACTTCACAAAGTCAATGCATTATCCACCTGCTAAATCAGCAAATATGGTCACCAATTTCATGGGAACCTCTTTTCTGCTCACCATCATGGGAGGCTTTATCAGTGACTCACTCTTCACTAGATTCACAACCTTCATTCTTTCCTGCATAATTGAGTTGCTGGGACTAATCTTGCTGACAATCCAATCTCAAAGCAGAAGGTTACGGCCGGCGTTGAAGTTAAAGCCTTCTCAGTCTCAAGCAGCAATTCTGTACACTGGCCTTTACGCCATTGCTGCTGGTGCTGCTGGACTCAATGCCAACTTACCTGCACATGGTGCTGATCAGCTTGATCACAGCAATCAGAGACAGATCTCTGCCTTCTTCAATTGGTTTTTCTTTTCCGTATGCACTGGTGGACTCATTAGCTCAACTGTCATGGTTTGGGTGGAAGAGAACAAAGGCTGGAATTGGAGCTTTCCCATAACAGTTATAGTTTTGAGTTTGGCGCTTCTTATCTTCATCAGTGGAAGTCCATTTTATAGGCACAAACGACCCACTGGGAGCTCATTAACAAGGATCATCAAGGTACTTACTTCTGCTGCTCAAAACTGGAAAGCCTCGCCAGATGAGAGATTGGGGAACCAGTATGCAGTAGCTGCACTCAACCTGAACAGAGCGGATGGAGATCACAAGTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

285

Amino Acids

31.62

Weight (kDa)

9.47

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 100 - 255 4.4e-41 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 211
Acc36I ACCTGC 2 cut(s) 211, 490
AccBSI CCGCTC 1 cut(s) 833
AciI CCGC 1 cut(s) 833
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 1 cut(s) 380
AdeI CACNNNGTG 1 cut(s) 491
AfaI GTAC 2 cut(s) 428, 744
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 2 cut(s) 391, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AluBI AGCT 6 cut(s) 101, 502, 582, 630, 720, 812
AluI AGCT 6 cut(s) 101, 502, 582, 630, 720, 812
Alw21I GWGCWC 1 cut(s) 722
Alw26I GTCTC 2 cut(s) 414, 514
AlwI GGATC 1 cut(s) 740
AlwNI CAGNNNCTG 1 cut(s) 812
AoxI GGCC 2 cut(s) 380, 433
ApeKI GCWGC 7 cut(s) 101, 104, 416, 449, 458, 755, 812
AspLEI GCGC 1 cut(s) 664
AsuHPI GGTGA 2 cut(s) 217, 247
BanII GRGCYC 1 cut(s) 722
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 7 cut(s) 88, 91, 428, 436, 445, 742, 799
BccI CCATC 2 cut(s) 266, 830
BceAI ACGGC 1 cut(s) 395
BclI TGATCA 3 cut(s) 40, 496, 505
BcoDI GTCTC 2 cut(s) 414, 514
BfaI CTAG 1 cut(s) 294
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 2 cut(s) 211, 490
BglII AGATCT 1 cut(s) 525
BisI GCNGC 7 cut(s) 102, 105, 417, 450, 459, 756, 813
BlsI GCNGC 7 cut(s) 103, 106, 418, 451, 460, 757, 814
BmiI GGNNCC 2 cut(s) 241, 797
BmrI ACTGGG 1 cut(s) 723
BmuI ACTGGG 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 840
BsaJI CCNNGG 1 cut(s) 85
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 6 cut(s) 159, 436, 571, 718, 770, 799
Bse3DI GCAATG 2 cut(s) 160, 444
Bse8I GATNNNNATC 1 cut(s) 840
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 1 cut(s) 841
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 2 cut(s) 160, 444
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 6 cut(s) 159, 436, 571, 718, 770, 799
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 7 cut(s) 88, 91, 428, 436, 445, 742, 799
BseYI CCCAGC 1 cut(s) 334
BsgI GTGCAG 2 cut(s) 468, 798
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 2 cut(s) 382, 435
BsiEI CGRYCG 1 cut(s) 383
BsiHKAI GWGCWC 1 cut(s) 722
BsiSI CCGG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 351
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 2 cut(s) 414, 514
BsmFI GGGAC 1 cut(s) 351
BsnI GGCC 2 cut(s) 382, 435
Bsp1286I GDGCHC 1 cut(s) 722
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 6 cut(s) 40, 496, 505, 525, 732, 841
Bsp19I CCATGG 1 cut(s) 85
BspACI CCGC 1 cut(s) 833
BspANI GGCC 2 cut(s) 382, 435
BspCNI CTCAG 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 241, 797
BspMI ACCTGC 2 cut(s) 211, 490
BspPI GGATC 1 cut(s) 740
BsrBI CCGCTC 1 cut(s) 833
BsrDI GCAATG 2 cut(s) 160, 444
BsrFI RCCGGY 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 6 cut(s) 159, 436, 571, 718, 770, 799
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 6 cut(s) 40, 496, 505, 525, 732, 841
BssT1I CCWWGG 1 cut(s) 85
Bst4CI ACNGT 2 cut(s) 589, 643
Bst6I CTCTTC 2 cut(s) 293, 600
BstAPI GCANNNNNTGC 2 cut(s) 491, 812
BstAUI TGTACA 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 405
BstDSI CCRYGG 1 cut(s) 85
BstEII GGTNACC 1 cut(s) 223
BstF5I GGATG 1 cut(s) 841
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 6 cut(s) 43, 499, 508, 528, 735, 844
BstMAI GTCTC 2 cut(s) 414, 514
BstMBI GATC 6 cut(s) 40, 496, 505, 525, 732, 841
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 7 cut(s) 110, 212, 449, 455, 458, 491, 812
BstNSI RCATGY 1 cut(s) 117
BstPI GGTNACC 1 cut(s) 223
BstV1I GCAGC 7 cut(s) 88, 91, 428, 436, 445, 742, 799
BstX2I RGATCY 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 235
BstYI RGATCY 1 cut(s) 525
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 382, 435
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 1 cut(s) 841
BtsIMutI CAGTG 5 cut(s) 283, 429, 564, 685, 711
BveI ACCTGC 2 cut(s) 211, 490
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 382
Csp6I GTAC 2 cut(s) 427, 743
CviAII CATG 7 cut(s) 86, 114, 139, 235, 262, 488, 592
CviQI GTAC 2 cut(s) 427, 743
DdeI CTNAG 1 cut(s) 405
DpnI GATC 6 cut(s) 42, 498, 507, 527, 734, 843
DpnII GATC 6 cut(s) 40, 496, 505, 525, 732, 841
DraIII CACNNNGTG 1 cut(s) 491
EaeI YGGCCR 1 cut(s) 380
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 293, 600
EarI CTCTTC 2 cut(s) 293, 600
Ecl136II GAGCTC 1 cut(s) 720
EclXI CGGCCG 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 1 cut(s) 722
Eco52I CGGCCG 1 cut(s) 380
Eco53kI GAGCTC 1 cut(s) 720
Eco91I GGTNACC 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 720
EcoO65I GGTNACC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 195
EcoT38I GRGCYC 1 cut(s) 722
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 7 cut(s) 89, 117, 142, 238, 265, 491, 595
FaqI GGGAC 1 cut(s) 351
FatI CATG 7 cut(s) 85, 113, 138, 234, 261, 487, 591
FbaI TGATCA 3 cut(s) 40, 496, 505
Fnu4HI GCNGC 7 cut(s) 102, 105, 417, 450, 459, 756, 813
FokI GGATG 1 cut(s) 848
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 7 cut(s) 102, 105, 417, 450, 459, 756, 813
FspBI CTAG 1 cut(s) 294
GlaI GCGC 1 cut(s) 663
GluI GCNGC 7 cut(s) 102, 105, 417, 450, 459, 756, 813
GsaI CCCAGC 1 cut(s) 338
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 2 cut(s) 382, 435
HapII CCGG 1 cut(s) 383
HhaI GCGC 1 cut(s) 664
Hin1II CATG 7 cut(s) 89, 117, 142, 238, 265, 491, 595
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HinfI GANTC 4 cut(s) 281, 297, 465, 573
HpaII CCGG 1 cut(s) 383
HphI GGTGA 2 cut(s) 217, 247
Hpy166II GTNNAC 2 cut(s) 429, 572
Hpy188I TCNGA 1 cut(s) 519
Hpy8I GTNNAC 2 cut(s) 429, 572
HpyAV CCTTC 4 cut(s) 316, 366, 411, 544
HpyCH4III ACNGT 2 cut(s) 589, 643
HpyCH4V TGCA 8 cut(s) 113, 153, 193, 321, 485, 564, 806, 815
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 212, 449, 455, 458, 491, 812
HpyF3I CTNAG 1 cut(s) 405
Hsp92II CATG 7 cut(s) 89, 117, 142, 238, 265, 491, 595
HspAI GCGC 1 cut(s) 662
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Ksp22I TGATCA 3 cut(s) 40, 496, 505
Kzo9I GATC 6 cut(s) 40, 496, 505, 525, 732, 841
LmnI GCTCC 2 cut(s) 627, 717
Lsp1109I GCAGC 7 cut(s) 88, 91, 428, 436, 445, 742, 799
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 5 cut(s) 161, 169, 223, 278, 375
MalI GATC 6 cut(s) 42, 498, 507, 527, 734, 843
MbiI CCGCTC 1 cut(s) 833
MboI GATC 6 cut(s) 40, 496, 505, 525, 732, 841
MboII GAAGA 5 cut(s) 75, 280, 529, 617, 664
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 722
MluCI AATT 6 cut(s) 93, 229, 324, 420, 541, 622
MlyI GAGTC 3 cut(s) 275, 459, 567
MnlI CCTC 4 cut(s) 118, 253, 260, 784
Mph1103I ATGCAT 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 382
MseI TTAA 3 cut(s) 395, 725, 852
MslI CAYNNNNRTG 1 cut(s) 260
MspI CCGG 1 cut(s) 383
MunI CAATTG 1 cut(s) 541
MwoI GCNNNNNNNGC 7 cut(s) 110, 212, 449, 455, 458, 491, 812
NaeI GCCGGC 1 cut(s) 384
NcoI CCATGG 1 cut(s) 85
NdeII GATC 6 cut(s) 40, 496, 505, 525, 732, 841
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 7 cut(s) 89, 117, 142, 238, 265, 491, 595
NlaIV GGNNCC 2 cut(s) 241, 797
NmuCI GTSAC 3 cut(s) 169, 223, 278
NsiI ATGCAT 1 cut(s) 195
NspI RCATGY 1 cut(s) 117
PaqCI CACCTGC 1 cut(s) 211
PdiI GCCGGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 297
PkrI GCNGC 7 cut(s) 103, 106, 418, 451, 460, 757, 814
PleI GAGTC 3 cut(s) 275, 459, 567
PpsI GAGTC 3 cut(s) 275, 459, 567
Psp124BI GAGCTC 1 cut(s) 722
PspEI GGTNACC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 334
PspN4I GGNNCC 2 cut(s) 241, 797
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 525
RsaI GTAC 2 cut(s) 428, 744
RsaNI GTAC 2 cut(s) 427, 743
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 722
SaqAI TTAA 3 cut(s) 395, 725, 852
SatI GCNGC 7 cut(s) 102, 105, 417, 450, 459, 756, 813
Sau3AI GATC 6 cut(s) 40, 496, 505, 525, 732, 841
SchI GAGTC 3 cut(s) 275, 459, 567
SduI GDGCHC 1 cut(s) 722
SmiMI CAYNNNNRTG 1 cut(s) 260
SmlI CTYRAG 1 cut(s) 411
SmoI CTYRAG 1 cut(s) 411
Sse9I AATT 6 cut(s) 93, 229, 324, 420, 541, 622
SsiI CCGC 1 cut(s) 833
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 722
StyI CCWWGG 1 cut(s) 85
TaaI ACNGT 2 cut(s) 589, 643
TasI AATT 6 cut(s) 93, 229, 324, 420, 541, 622
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 3 cut(s) 395, 725, 852
Tru9I TTAA 3 cut(s) 395, 725, 852
TscAI CASTG 5 cut(s) 283, 436, 571, 685, 718
TseFI GTSAC 3 cut(s) 169, 223, 278
TseI GCWGC 7 cut(s) 101, 104, 416, 449, 458, 755, 812
Tsp45I GTSAC 3 cut(s) 169, 223, 278
TspDTI ATGAA 3 cut(s) 223, 298, 664
TspGWI ACGGA 1 cut(s) 547
TspRI CASTG 5 cut(s) 283, 436, 571, 685, 718
XceI RCATGY 1 cut(s) 117
XspI CTAG 1 cut(s) 294
Zsp2I ATGCAT 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.