RchiOBHm_Chr6g0296871

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006780
Physical Location & Seq
Forward (+)
58566031 .. 58566513
483 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26648

Sequence Viewer

Length: 483 bp
ATGTCTCCCCTGAGCAGCAATGATTTAGAGCGAATTTTCGAGAAGCTTGACAAGAACGGAGATGGCCAAGTGAGTGTCGAGGAGCTCAACTGGCTCCTCGAGAGAATTGGGGTCGAGTTCAGCCTGCAGGAGATCGAGTCCCTGGTGGGCAAACCGAGCCTCGACTGGAATGACTTCTTGTTCTTCTACAGGTCCATATCTACGCCGGATAGTAATGACGGCGACAATGGCCATGGAGATGGTAGCGCCAATGATCATGACGATGAGAGTGATCTTGTGAAGGCGTTCGATGTGTTCGACATAAACGGCGACGGCTTCATTTCTTGTGAGGAGCTTGAGAGCGTTCTGAGGAGATTAGGAGTAATGGAAGAGAATAGTAGCCGTGACTGCAGATCCATGATTCACGTGTACGACACCAATTTGGACGGGCTACTTGATTTCGAGGAATTCAAACACATGATGTTGCATAATACCATTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

160

Amino Acids

18.17

Weight (kDa)

4.18

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 7 - 51 1.1e-06 EF-hand domain pair
EF-hand_1 PF00036 8 - 34 2.9e-06 EF hand domain
EF-hand_5 PF13202 10 - 30 4.5e-06 EF hand
EF-hand_7 PF13499 89 - 154 8.2e-14 EF-hand domain pair
EF-hand_1 PF00036 92 - 119 4.7e-07 EF hand domain
EF-hand_6 PF13405 92 - 120 1.9e-07 EF-hand domain
EF-hand_5 PF13202 93 - 115 1.5e-06 EF hand
EF-hand_1 PF00036 129 - 154 3.8e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 387
AcoI YGGCCR 2 cut(s) 64, 229
AcsI RAATTY 2 cut(s) 33, 446
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 3 cut(s) 46, 85, 334
AluI AGCT 3 cut(s) 46, 85, 334
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 387
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 2 cut(s) 64, 229
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 2 cut(s) 33, 446
Asp700I GAANNNNTTC 2 cut(s) 173, 284
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 192
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 2 cut(s) 66, 231
BanII GRGCYC 1 cut(s) 87
BbrPI CACGTG 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 27
BccI CCATC 2 cut(s) 56, 233
BceAI ACGGC 4 cut(s) 235, 322, 328, 366
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 9
BfmI CTRYAG 3 cut(s) 125, 187, 388
BfoI RGCGCY 1 cut(s) 249
BisI GCNGC 1 cut(s) 16
BlsI GCNGC 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 11
BpuEI CTTGAG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 141, 232
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse1I ACTGG 2 cut(s) 95, 170
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 2 cut(s) 141, 232
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 2 cut(s) 95, 170
BseRI GAGGAG 4 cut(s) 86, 95, 344, 364
BseXI GCAGC 1 cut(s) 27
BshFI GGCC 2 cut(s) 66, 231
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 124
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 2 cut(s) 66, 231
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 4 cut(s) 132, 253, 271, 392
Bsp19I CCATGG 1 cut(s) 232
BspANI GGCC 2 cut(s) 66, 231
BspCNI CTCAG 1 cut(s) 339
BspHI TCATGA 1 cut(s) 256
BspLI GGNNCC 1 cut(s) 95
BspMAI CTGCAG 2 cut(s) 129, 392
BspPI GGATC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 2 cut(s) 95, 170
BssECI CCNNGG 2 cut(s) 141, 232
BssMI GATC 4 cut(s) 132, 253, 271, 392
BssT1I CCWWGG 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 363
BstBAI YACGTR 1 cut(s) 406
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 3 cut(s) 11, 347, 480
BstDSI CCRYGG 1 cut(s) 232
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 4 cut(s) 135, 256, 274, 395
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 132, 253, 271, 392
BstMWI GCNNNNNNNGC 4 cut(s) 91, 156, 228, 387
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 3 cut(s) 125, 187, 388
BstV1I GCAGC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 392
BstXI CCANNNNNNTGG 1 cut(s) 239
BstYI RGATCY 1 cut(s) 392
BsuRI GGCC 2 cut(s) 66, 231
BtgI CCRYGG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 125
CciI TCATGA 1 cut(s) 256
CfoI GCGC 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 409
CviAII CATG 4 cut(s) 233, 257, 397, 457
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 3 cut(s) 11, 347, 480
DpnI GATC 4 cut(s) 134, 255, 273, 394
DpnII GATC 4 cut(s) 132, 253, 271, 392
EaeI YGGCCR 2 cut(s) 64, 229
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 85
Eco72I CACGTG 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 85
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 232
FaeI CATG 4 cut(s) 236, 260, 400, 460
FaiI YATR 7 cut(s) 197, 234, 258, 302, 398, 458, 468
FaqI GGGAC 1 cut(s) 124
FatI CATG 4 cut(s) 232, 256, 396, 456
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 1 cut(s) 16
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 1 cut(s) 16
GlaI GCGC 1 cut(s) 247
GluI GCNGC 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 2 cut(s) 66, 231
HapII CCGG 1 cut(s) 206
HhaI GCGC 1 cut(s) 248
Hin1II CATG 4 cut(s) 236, 260, 400, 460
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 2 cut(s) 137, 400
HpaII CCGG 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 3 cut(s) 40, 100, 257
Hpy8I GTNNAC 1 cut(s) 409
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 3 cut(s) 127, 390, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 91, 156, 228, 387
HpyF3I CTNAG 3 cut(s) 11, 347, 480
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 4 cut(s) 236, 260, 400, 460
HspAI GCGC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 4 cut(s) 132, 253, 271, 392
LmnI GCTCC 3 cut(s) 82, 99, 331
LpnPI CCDG 9 cut(s) 23, 76, 113, 128, 137, 151, 155, 175, 219
Lsp1109I GCAGC 1 cut(s) 27
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 1 cut(s) 383
MalI GATC 4 cut(s) 134, 255, 273, 394
MboI GATC 4 cut(s) 132, 253, 271, 392
MboII GAAGA 2 cut(s) 175, 380
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 1 cut(s) 87
MlsI TGGCCA 2 cut(s) 66, 231
MluCI AATT 4 cut(s) 33, 105, 418, 446
MluNI TGGCCA 2 cut(s) 66, 231
MlyI GAGTC 1 cut(s) 146
MnlI CCTC 6 cut(s) 73, 107, 170, 322, 342, 436
Mox20I TGGCCA 2 cut(s) 66, 231
MroXI GAANNNNTTC 2 cut(s) 173, 284
MscI TGGCCA 2 cut(s) 66, 231
MslI CAYNNNNRTG 2 cut(s) 237, 261
Msp20I TGGCCA 2 cut(s) 66, 231
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 91, 156, 228, 387
NcoI CCATGG 1 cut(s) 232
NdeII GATC 4 cut(s) 132, 253, 271, 392
NlaIII CATG 4 cut(s) 236, 260, 400, 460
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdmI GAANNNNTTC 2 cut(s) 173, 284
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 1 cut(s) 17
PleI GAGTC 1 cut(s) 145
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 406
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 141
PspCI CACGTG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 95
PspPI GGNCC 1 cut(s) 192
PstI CTGCAG 2 cut(s) 129, 392
PsuI RGATCY 1 cut(s) 392
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 2 cut(s) 237, 261
SacI GAGCTC 1 cut(s) 87
SatI GCNGC 1 cut(s) 16
Sau3AI GATC 4 cut(s) 132, 253, 271, 392
Sau96I GGNCC 1 cut(s) 192
SbfI CCTGCAGG 1 cut(s) 129
SchI GAGTC 1 cut(s) 146
ScrFI CCNGG 1 cut(s) 143
SdaI CCTGCAGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 87
SetI ASST 5 cut(s) 48, 87, 194, 336, 408
SfcI CTRYAG 3 cut(s) 125, 187, 388
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 237, 261
SmlI CTYRAG 2 cut(s) 98, 335
SmoI CTYRAG 2 cut(s) 98, 335
Sse8387I CCTGCAGG 1 cut(s) 129
Sse9I AATT 4 cut(s) 33, 105, 418, 446
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 1 cut(s) 232
TaiI ACGT 1 cut(s) 408
TaqI TCGA 9 cut(s) 39, 78, 99, 114, 135, 162, 288, 297, 441
TasI AATT 4 cut(s) 33, 105, 418, 446
TfiI GAWTC 1 cut(s) 400
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 15
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 72
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 2 cut(s) 33, 446
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 2 cut(s) 173, 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.