MD08G1159800.v1.1

Calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006780
Physical Location & Seq
Reverse (-)
18382729 .. 18383061
333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1159800.v1.1.491

Sequence Viewer

Length: 333 bp
ATGAACGAGTTCTTGTTCTTCTACAAGTCCATATCAATGCAGCAAAGTGGCGCCGGTGACAAAAACGAAGGCAGTGACACTCGTGGGGATGTCGCTGTTGATCATGAAGATGAAGATGAGGACCTTGCGAAGGCATTCATTGTGTTTGATTTGAACGGCGATGGCCTCATCTCTAGCGAGGAGTTTGAGATCGTACTGAAGAGATTAGGGGTTTTGGAGGAGAATAGCAGCCGTGACTGTAGAACTATGATTCACGTGTACGACACCTACTTGGACGGCCTCCTTGATATTCTGGAATTCAAAACCATGATGTTCCAAAATACTATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 ko04626 map03018 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

111

Amino Acids

12.52

Weight (kDa)

4.17

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 40 - 104 4.5e-08 EF-hand domain pair
EF-hand_1 PF00036 42 - 69 4.1e-06 EF hand domain
EF-hand_5 PF13202 43 - 64 1.3e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AcsI RAATTY 1 cut(s) 296
AcuI CTGAAG 1 cut(s) 218
AcvI CACGTG 1 cut(s) 256
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 195, 260
AfiI CCNNNNNNNGG 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 2 cut(s) 154, 301
AoxI GGCC 2 cut(s) 163, 277
ApeKI GCWGC 2 cut(s) 40, 228
ApoI RAATTY 1 cut(s) 296
Asp700I GAANNNNTTC 2 cut(s) 8, 134
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 121
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 50
BauI CACGAG 1 cut(s) 81
BbrPI CACGTG 1 cut(s) 256
BbvI GCAGC 2 cut(s) 52, 240
BccI CCATC 1 cut(s) 155
BceAI ACGGC 3 cut(s) 172, 216, 292
BclI TGATCA 1 cut(s) 100
BfaI CTAG 1 cut(s) 174
BfmI CTRYAG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 2 cut(s) 41, 229
BlsI GCNGC 2 cut(s) 42, 230
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse118I RCCGGY 1 cut(s) 53
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 1 cut(s) 130
BseRI GAGGAG 2 cut(s) 194, 233
BseXI GCAGC 2 cut(s) 52, 240
BshFI GGCC 2 cut(s) 165, 279
BshNI GGYRCC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 130
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 2 cut(s) 165, 279
Bsp143I GATC 2 cut(s) 100, 189
BspANI GGCC 2 cut(s) 165, 279
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 1 cut(s) 52
BspT107I GGYRCC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssMI GATC 2 cut(s) 100, 189
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 239
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 51
BstBAI YACGTR 1 cut(s) 256
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 1 cut(s) 94
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 2 cut(s) 103, 192
BstMBI GATC 2 cut(s) 100, 189
BstSFI CTRYAG 1 cut(s) 238
BstV1I GCAGC 2 cut(s) 52, 240
BsuRI GGCC 2 cut(s) 165, 279
BtgZI GCGATG 1 cut(s) 174
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 1 cut(s) 79
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 121
Csp6I GTAC 2 cut(s) 194, 259
CviAII CATG 2 cut(s) 104, 307
CviJI RGCY 3 cut(s) 165, 231, 279
CviKI_1 RGCY 3 cut(s) 165, 231, 279
CviQI GTAC 2 cut(s) 194, 259
DinI GGCGCC 1 cut(s) 52
DpnI GATC 2 cut(s) 102, 191
DpnII GATC 2 cut(s) 100, 189
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 218
Eco72I CACGTG 1 cut(s) 256
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 296
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
FaeI CATG 2 cut(s) 107, 310
FaiI YATR 4 cut(s) 32, 105, 248, 308
FatI CATG 2 cut(s) 103, 306
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 41, 229
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 41, 229
FspBI CTAG 1 cut(s) 174
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 41, 229
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 2 cut(s) 165, 279
HapII CCGG 1 cut(s) 54
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 2 cut(s) 107, 310
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 1 cut(s) 250
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 259
Hpy188III TCNNGA 2 cut(s) 104, 293
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 62, 124
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 1 cut(s) 40
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 2 cut(s) 107, 310
HspAI GCGC 1 cut(s) 51
KasI GGCGCC 1 cut(s) 50
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 2 cut(s) 100, 189
LpnPI CCDG 2 cut(s) 67, 278
Lsp1109I GCAGC 2 cut(s) 52, 240
MaeI CTAG 1 cut(s) 174
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 56, 74, 233
MalI GATC 2 cut(s) 102, 191
MboI GATC 2 cut(s) 100, 189
MboII GAAGA 4 cut(s) 10, 119, 125, 211
MluCI AATT 1 cut(s) 296
Mly113I GGCGCC 1 cut(s) 51
MnlI CCTC 5 cut(s) 112, 172, 176, 211, 290
MroXI GAANNNNTTC 2 cut(s) 8, 134
MseI TTAA 1 cut(s) 331
MslI CAYNNNNRTG 2 cut(s) 35, 108
MspI CCGG 1 cut(s) 54
Mva1269I GAATGC 1 cut(s) 134
NarI GGCGCC 1 cut(s) 51
NdeII GATC 2 cut(s) 100, 189
NlaIII CATG 2 cut(s) 107, 310
NlaIV GGNNCC 1 cut(s) 52
NmuCI GTSAC 3 cut(s) 56, 74, 233
PagI TCATGA 1 cut(s) 103
PctI GAATGC 1 cut(s) 134
PdmI GAANNNNTTC 2 cut(s) 8, 134
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 2 cut(s) 42, 230
PluTI GGCGCC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
Ppu21I YACGTR 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
PspCI CACGTG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 52
PspPI GGNCC 1 cut(s) 121
PspPPI RGGWCCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 195, 260
RsaNI GTAC 2 cut(s) 194, 259
RseI CAYNNNNRTG 2 cut(s) 35, 108
SaqAI TTAA 1 cut(s) 331
SatI GCNGC 2 cut(s) 41, 229
Sau3AI GATC 2 cut(s) 100, 189
Sau96I GGNCC 1 cut(s) 121
SetI ASST 3 cut(s) 126, 258, 269
SfcI CTRYAG 1 cut(s) 238
SfoI GGCGCC 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 53
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 2 cut(s) 35, 108
Sse9I AATT 1 cut(s) 296
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 1 cut(s) 174
TaaI ACNGT 1 cut(s) 239
TaiI ACGT 1 cut(s) 258
TasI AATT 1 cut(s) 296
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 1 cut(s) 331
Tru9I TTAA 1 cut(s) 331
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 3 cut(s) 56, 74, 233
TseI GCWGC 2 cut(s) 40, 228
Tsp45I GTSAC 3 cut(s) 56, 74, 233
TspDTI ATGAA 4 cut(s) 17, 120, 126, 127
TspRI CASTG 1 cut(s) 79
VpaK11BI GGWCC 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 1 cut(s) 296
XmnI GAANNNNTTC 2 cut(s) 8, 134
XspI CTAG 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.