Prupe.1G488600_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0006780
Physical Location & Seq
Reverse (-)
40682061 .. 40682558
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G488600.1

Sequence Viewer

Length: 498 bp
ATGTGTCCTCTAAGCACCAACGACTTGCTTCGAATTTTCGAGATGCTCGACAGGAATGGTGACGGGCAAGTGAGTCTCGAGGAGCTCAGCTGGCTCCTCGAGAGGATTGGCGTGCAGTTCAGCCTGAACGAGCTCGAGTCCTCGGTGGGGAAACCGAGCCTCGACTTCAATGAATTCTTGTTCTTCTACAAGTCCATATCCATGCAGCAAAATTACAAGAGTGACGACGATCGCCATGAAGATGGTAACATTCATGAGGAGGTTGTCCCTGATGAAGATGAGAGTGACCTTGTGAAGGCATTCAATGTGTTTGATTTGAATGGCGATGGCTTCATTTCCTGTGAGGAGCTGGGGAGCGTGCTGAGGAGATTAGGAGTTTTGGAGGAGAATAGCAGCCGTGACTGCAAGACCATGATTCACGTGTACGACACCAACTTGGATGGCCTGCTTGATTTTCAGGAATTCAAAAACATGATGTTTCAAAATACCATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

166

Amino Acids

19.01

Weight (kDa)

4.2

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 33, 173, 461
AcvI CACGTG 1 cut(s) 421
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 2 cut(s) 147, 295
AflIII ACRYGT 1 cut(s) 420
AgsI TTSAA 5 cut(s) 169, 304, 319, 466, 482
AluBI AGCT 4 cut(s) 85, 90, 133, 349
AluI AGCT 4 cut(s) 85, 90, 133, 349
Alw21I GWGCWC 2 cut(s) 87, 135
Alw26I GTCTC 1 cut(s) 80
Ama87I CYCGRG 3 cut(s) 77, 98, 134
AoxI GGCC 1 cut(s) 442
ApeKI GCWGC 2 cut(s) 205, 393
ApoI RAATTY 3 cut(s) 33, 173, 461
Asp700I GAANNNNTTC 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 71
AsuII TTCGAA 1 cut(s) 31
AvaI CYCGRG 3 cut(s) 77, 98, 134
BanII GRGCYC 2 cut(s) 87, 135
BbrPI CACGTG 1 cut(s) 421
Bbv12I GWGCWC 2 cut(s) 87, 135
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 217, 405
BccI CCATC 4 cut(s) 236, 320, 434, 497
BceAI ACGGC 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 80
BisI GCNGC 2 cut(s) 206, 394
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 2 cut(s) 207, 395
BmeT110I CYCGRG 3 cut(s) 77, 98, 134
BmiI GGNNCC 1 cut(s) 95
BmsI GCATC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 31
BsaAI YACGTR 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 295
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 2 cut(s) 147, 295
BseMII CTCAG 2 cut(s) 100, 353
BseRI GAGGAG 6 cut(s) 86, 95, 272, 359, 379, 398
BseXI GCAGC 2 cut(s) 217, 405
BseYI CCCAGC 1 cut(s) 349
BsgI GTGCAG 1 cut(s) 134
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 444
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 2 cut(s) 87, 135
BsiHKCI CYCGRG 3 cut(s) 77, 98, 134
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 147, 295
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 299
BsnI GGCC 1 cut(s) 444
BsoBI CYCGRG 3 cut(s) 77, 98, 134
Bsp119I TTCGAA 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 87, 135
Bsp143I GATC 1 cut(s) 229
Bsp1720I GCTNAGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 444
BspCNI CTCAG 2 cut(s) 99, 354
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 1 cut(s) 229
BstBAI YACGTR 1 cut(s) 421
BstBI TTCGAA 1 cut(s) 31
BstC8I GCNNGC 4 cut(s) 92, 113, 359, 446
BstDEI CTNAG 3 cut(s) 11, 86, 362
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 1 cut(s) 229
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 2 cut(s) 91, 402
BstV1I GCAGC 2 cut(s) 217, 405
BstXI CCANNNNNNTGG 1 cut(s) 242
BsuRI GGCC 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 1 cut(s) 445
Cac8I GCNNGC 4 cut(s) 92, 113, 359, 446
CciI TCATGA 1 cut(s) 253
Csp6I GTAC 1 cut(s) 424
CviAII CATG 5 cut(s) 202, 236, 254, 412, 472
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 3 cut(s) 11, 86, 362
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
Ecl136II GAGCTC 2 cut(s) 85, 133
Eco24I GRGCYC 2 cut(s) 87, 135
Eco53kI GAGCTC 2 cut(s) 85, 133
Eco72I CACGTG 1 cut(s) 421
Eco88I CYCGRG 3 cut(s) 77, 98, 134
EcoICRI GAGCTC 2 cut(s) 85, 133
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRI GAATTC 2 cut(s) 173, 461
EcoT38I GRGCYC 2 cut(s) 87, 135
FaeI CATG 5 cut(s) 205, 239, 257, 415, 475
FaiI YATR 6 cut(s) 197, 203, 237, 255, 413, 473
FaqI GGGAC 1 cut(s) 251
FatI CATG 5 cut(s) 201, 235, 253, 411, 471
Fnu4HI GCNGC 2 cut(s) 206, 394
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 2 cut(s) 87, 135
Fsp4HI GCNGC 2 cut(s) 206, 394
GluI GCNGC 2 cut(s) 206, 394
GsaI CCCAGC 1 cut(s) 353
HaeIII GGCC 1 cut(s) 444
Hin1II CATG 5 cut(s) 205, 239, 257, 415, 475
HinfI GANTC 3 cut(s) 73, 137, 415
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 424
Hpy188III TCNNGA 5 cut(s) 40, 77, 100, 254, 458
Hpy8I GTNNAC 1 cut(s) 424
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 3 cut(s) 115, 205, 405
HpyF10VI GCNNNNNNNGC 2 cut(s) 91, 402
HpyF3I CTNAG 3 cut(s) 11, 86, 362
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 5 cut(s) 205, 239, 257, 415, 475
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 4 cut(s) 82, 99, 346, 354
LpnPI CCDG 8 cut(s) 37, 76, 137, 282, 335, 352, 443, 458
Lsp1109I GCAGC 2 cut(s) 217, 405
LweI GCATC 1 cut(s) 33
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 5 cut(s) 59, 221, 245, 284, 398
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 3 cut(s) 175, 251, 287
MhlI GDGCHC 2 cut(s) 87, 135
MluCI AATT 4 cut(s) 33, 173, 211, 461
MlyI GAGTC 2 cut(s) 82, 146
MroXI GAANNNNTTC 1 cut(s) 299
MseI TTAA 1 cut(s) 496
MslI CAYNNNNRTG 2 cut(s) 200, 240
MspA1I CMGCKG 1 cut(s) 90
Mva1269I GAATGC 1 cut(s) 299
MwoI GCNNNNNNNGC 2 cut(s) 91, 402
NdeII GATC 1 cut(s) 229
NlaIII CATG 5 cut(s) 205, 239, 257, 415, 475
NlaIV GGNNCC 1 cut(s) 95
NmuCI GTSAC 4 cut(s) 59, 221, 284, 398
NspV TTCGAA 1 cut(s) 31
PaeR7I CTCGAG 3 cut(s) 77, 98, 134
PagI TCATGA 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PctI GAATGC 1 cut(s) 299
PdmI GAANNNNTTC 1 cut(s) 299
PfeI GAWTC 1 cut(s) 415
PkrI GCNGC 2 cut(s) 207, 395
Ple19I CGATCG 1 cut(s) 232
PleI GAGTC 2 cut(s) 81, 145
PmaCI CACGTG 1 cut(s) 421
PmlI CACGTG 1 cut(s) 421
PpsI GAGTC 2 cut(s) 81, 145
Ppu21I YACGTR 1 cut(s) 421
Psp124BI GAGCTC 2 cut(s) 87, 135
PspCI CACGTG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 95
PspXI VCTCGAGB 1 cut(s) 134
PvuI CGATCG 1 cut(s) 232
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 2 cut(s) 200, 240
SacI GAGCTC 2 cut(s) 87, 135
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 2 cut(s) 206, 394
Sau3AI GATC 1 cut(s) 229
SchI GAGTC 2 cut(s) 82, 146
SduI GDGCHC 2 cut(s) 87, 135
SetI ASST 7 cut(s) 87, 92, 135, 264, 291, 351, 423
SfaNI GCATC 1 cut(s) 33
Sfr274I CTCGAG 3 cut(s) 77, 98, 134
SfuI TTCGAA 1 cut(s) 31
SlaI CTCGAG 3 cut(s) 77, 98, 134
SmiMI CAYNNNNRTG 2 cut(s) 200, 240
SmlI CTYRAG 3 cut(s) 77, 98, 134
SmoI CTYRAG 3 cut(s) 77, 98, 134
Sse9I AATT 4 cut(s) 33, 173, 211, 461
SstI GAGCTC 2 cut(s) 87, 135
TaiI ACGT 1 cut(s) 423
TaqI TCGA 7 cut(s) 31, 39, 48, 78, 99, 135, 162
TasI AATT 4 cut(s) 33, 173, 211, 461
TfiI GAWTC 1 cut(s) 415
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TseFI GTSAC 4 cut(s) 59, 221, 284, 398
TseI GCWGC 2 cut(s) 205, 393
Tsp45I GTSAC 4 cut(s) 59, 221, 284, 398
TspDTI ATGAA 5 cut(s) 186, 242, 252, 288, 322
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 3 cut(s) 33, 173, 461
XhoI CTCGAG 3 cut(s) 77, 98, 134
XmnI GAANNNNTTC 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.