Rorug06G0266800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000931
Physical Location & Seq
Reverse (-)
41330805 .. 41334684
3880 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0266800.1

Sequence Viewer

Length: 1143 bp
ATGGCTTCTTCTTCTTCTTGCGCTAACCAGAACTTGGCGACGCTAATCTCACTGACTGTGATTCTGCTAATCTCACCCGCGTATTCCCAGAGCTGCTCCTCCCAGTCCTTCGCCAACAAAATGCTCTACACCCAATGCGCCGACCTCCCAACCCTCAGCTCTACCCTCCACTGGTACTACGACTCCGCCAACTCCACTCTCGCCATCGCCTTCATCGCGCCGCCGGCCAAGCCCGCCGGATGGACTTCCTGGGCCATCAACCCCACCGGCACCGGCATGGCCGGGGCCCAAACGCTCCTCGCCTACAAAACCTCCTCCGGCGCCATGGCCGTCAAGACCTTCAACATCAGCTCCTACAGCTCCATCGTACCCGGAAAGCTCGCCTTCGACGTCTGGGACACGGCCGCTGAGTCCTCCGGCGGGGTCTTCCGGTTGTTTGCCAAGGTGAAGGTGAAGACTGGGCAGGTGAACCACGTCTGGCAGGTGGGGCCGTCCGTCATAGATGGGTTTCCGGCGAAGCATGCTTTTGATGCACCCAATCTTCAGGCTAAGGGGACTCTCACCTTGACCGGAACTAATGCCGGTGTCACCGGGGCCGGAGCCGATCCCAAGTTGAAGGACAGGAATATTCATGGAATTCTGAATGCTGTGAGTTGGGGGTTTTTGTTTCCACTGGGGATTATTATAGCAAGGTACATCAGGACTTTTCCGTCTGCGGATCCTGCATGGTTTTATCTCCATATATCCTGCCAAATATCTGCTTATGCCATCGGTGTCGCCGGCTGGGCCACCGGACTGAAGCTTGGAAGGGGTGAATACACTACCCACCGCAACATCGGAATTACCCTCTTCACCTTTGCAACTGTGCAGATGTTTGCATTGTTCTTGAGACCTGGTAAGGATCACAAGTACAGGCTCTACTGGAACATCTACCACCACAGCGTTGGATATGCGATTCTAGTGCTTGGCATTGTGAATATTTTCAAAGGCTTAGACATACTAAATCCTACTAACCATTGGAAAACCGCTTACATAATTGCCATCTGTGTGTTGGGTGGGATCGCCGTAGTCTTGGAAATATTGACTTGGATTGTTGTACTAAACAGAAGGTCAAGTAAGTCCACCAAGCCATATGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

380

Amino Acids

41.01

Weight (kDa)

9.54

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 93 - 189 4.6e-27 Protein of unknown function (DUF568)
Cytochrom_B561 PF03188 211 - 331 3.3e-07 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 454, 472
AasI GACNNNNNNGTC 1 cut(s) 709
AatII GACGTC 1 cut(s) 393
Acc36I ACCTGC 2 cut(s) 454, 472
AccB1I GGYRCC 2 cut(s) 269, 320
AccB7I CCANNNNNTGG 2 cut(s) 34, 1136
AccII CGCG 2 cut(s) 80, 218
AciI CCGC 9 cut(s) 78, 186, 221, 234, 405, 420, 716, 829, 1026
AclWI GGATC 5 cut(s) 599, 713, 726, 909, 1067
AcoI YGGCCR 4 cut(s) 225, 279, 327, 402
AcsI RAATTY 1 cut(s) 636
AcuI CTGAAG 2 cut(s) 527, 818
AcyI GRCGYC 2 cut(s) 321, 390
AfaI GTAC 5 cut(s) 176, 369, 695, 911, 1098
AfiI CCNNNNNNNGG 5 cut(s) 34, 171, 240, 420, 1136
AgsI TTSAA 3 cut(s) 343, 616, 985
AjiI CACGTC 1 cut(s) 475
AjnI CCWGG 2 cut(s) 248, 892
AluBI AGCT 6 cut(s) 93, 159, 351, 360, 379, 802
AluI AGCT 6 cut(s) 93, 159, 351, 360, 379, 802
Alw26I GTCTC 1 cut(s) 883
AlwI GGATC 5 cut(s) 599, 713, 726, 909, 1067
AoxI GGCC 9 cut(s) 225, 252, 279, 285, 327, 402, 488, 594, 786
ApaI GGGCCC 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 93
ApoI RAATTY 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 980
AspLEI GCGC 4 cut(s) 23, 140, 220, 323
AspS9I GGNCC 6 cut(s) 252, 285, 286, 488, 594, 786
AsuC2I CCSGG 3 cut(s) 283, 372, 592
AsuHPI GGTGA 8 cut(s) 66, 457, 463, 478, 553, 580, 824, 844
BaeGI GKGCMC 1 cut(s) 289
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 2 cut(s) 269, 320
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 2 cut(s) 418, 461
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 80
BccI CCATC 8 cut(s) 212, 234, 263, 371, 497, 776, 1049, 1130
BceAI ACGGC 4 cut(s) 314, 417, 475, 1049
BcgI CGANNNNNNTGC 2 cut(s) 943, 977
BciT130I CCWGG 2 cut(s) 250, 894
BcnI CCSGG 3 cut(s) 283, 372, 592
BcoDI GTCTC 1 cut(s) 883
BfaI CTAG 1 cut(s) 959
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 324
BfuAI ACCTGC 2 cut(s) 454, 472
BglI GCCNNNNNGGC 1 cut(s) 785
BisI GCNGC 3 cut(s) 94, 221, 405
BlsI GCNGC 3 cut(s) 95, 222, 406
Bme1390I CCNGG 5 cut(s) 250, 283, 372, 592, 894
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 6 cut(s) 252, 285, 286, 488, 594, 786
BmiI GGNNCC 8 cut(s) 271, 286, 287, 322, 489, 595, 601, 720
BmrFI CCNGG 5 cut(s) 250, 283, 372, 592, 894
BmrI ACTGGG 3 cut(s) 97, 468, 683
BmsI GCATC 1 cut(s) 520
BmuI ACTGGG 3 cut(s) 97, 468, 683
BpiI GAAGAC 2 cut(s) 418, 461
Bpu10I CCTNAGC 2 cut(s) 155, 549
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 3 cut(s) 283, 372, 592
BsaHI GRCGYC 2 cut(s) 321, 390
BsaI GGTCTC 1 cut(s) 883
BsaJI CCNNGG 5 cut(s) 249, 282, 324, 441, 591
BsaWI WCCGGW 3 cut(s) 429, 569, 791
BsaXI ACNNNNNCTCC 6 cut(s) 167, 197, 296, 326, 335, 365
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 171, 240, 420, 1136
Bse118I RCCGGY 5 cut(s) 223, 266, 272, 581, 779
Bse1I ACTGG 5 cut(s) 103, 176, 463, 678, 926
BseBI CCWGG 2 cut(s) 250, 894
BseDI CCNNGG 5 cut(s) 249, 282, 324, 441, 591
BseGI GGATG 1 cut(s) 245
BseLI CCNNNNNNNGG 5 cut(s) 34, 171, 240, 420, 1136
BseMII CTCAG 2 cut(s) 169, 399
BseNI ACTGG 5 cut(s) 103, 176, 463, 678, 926
BseRI GAGGAG 3 cut(s) 88, 287, 304
BseSI GKGCMC 1 cut(s) 289
BseX3I CGGCCG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 80
BseYI CCCAGC 1 cut(s) 783
BsgI GTGCAG 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 80, 218
Bsh1285I CGRYCG 1 cut(s) 405
BshFI GGCC 9 cut(s) 227, 254, 281, 287, 329, 404, 490, 596, 788
BshNI GGYRCC 2 cut(s) 269, 320
BsiEI CGRYCG 1 cut(s) 405
BslFI GGGAC 2 cut(s) 410, 568
BslI CCNNNNNNNGG 5 cut(s) 34, 171, 240, 420, 1136
BsmAI GTCTC 1 cut(s) 883
BsmFI GGGAC 2 cut(s) 410, 568
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 9 cut(s) 227, 254, 281, 287, 329, 404, 490, 596, 788
Bso31I GGTCTC 1 cut(s) 883
Bsp120I GGGCCC 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 4 cut(s) 604, 718, 901, 1059
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 9 cut(s) 78, 186, 221, 234, 405, 420, 716, 829, 1026
BspANI GGCC 9 cut(s) 227, 254, 281, 287, 329, 404, 490, 596, 788
BspCNI CTCAG 2 cut(s) 168, 400
BspFNI CGCG 2 cut(s) 80, 218
BspLI GGNNCC 8 cut(s) 271, 286, 287, 322, 489, 595, 601, 720
BspMI ACCTGC 2 cut(s) 454, 472
BspPI GGATC 5 cut(s) 599, 713, 726, 909, 1067
BspT107I GGYRCC 2 cut(s) 269, 320
BspTNI GGTCTC 1 cut(s) 883
BsrFI RCCGGY 5 cut(s) 223, 266, 272, 581, 779
BsrI ACTGG 5 cut(s) 103, 176, 463, 678, 926
BssAI RCCGGY 5 cut(s) 223, 266, 272, 581, 779
BssECI CCNNGG 5 cut(s) 249, 282, 324, 441, 591
BssMI GATC 4 cut(s) 604, 718, 901, 1059
BssNI GRCGYC 2 cut(s) 321, 390
BssT1I CCWWGG 2 cut(s) 324, 441
Bst2UI CCWGG 2 cut(s) 250, 894
Bst4CI ACNGT 2 cut(s) 58, 865
Bst6I CTCTTC 1 cut(s) 854
BstACI GRCGYC 2 cut(s) 321, 390
BstC8I GCNNGC 5 cut(s) 225, 234, 381, 522, 781
BstDEI CTNAG 4 cut(s) 155, 408, 549, 991
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 245
BstFNI CGCG 2 cut(s) 80, 218
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 4 cut(s) 23, 140, 220, 323
BstKTI GATC 4 cut(s) 607, 721, 904, 1062
BstMAI GTCTC 1 cut(s) 883
BstMBI GATC 4 cut(s) 604, 718, 901, 1059
BstMCI CGRYCG 1 cut(s) 405
BstNI CCWGG 2 cut(s) 250, 894
BstNSI RCATGY 1 cut(s) 524
BstSCI CCNGG 5 cut(s) 248, 281, 370, 590, 892
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 289
BstUI CGCG 2 cut(s) 80, 218
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 2 cut(s) 418, 461
BstX2I RGATCY 1 cut(s) 718
BstXI CCANNNNNNTGG 1 cut(s) 944
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 402
BsuRI GGCC 9 cut(s) 227, 254, 281, 287, 329, 404, 490, 596, 788
BtgI CCRYGG 1 cut(s) 324
BtgZI GCGATG 2 cut(s) 190, 199
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 1 cut(s) 245
BtsIMutI CAGTG 3 cut(s) 50, 169, 671
BveI ACCTGC 2 cut(s) 454, 472
Cac8I GCNNGC 5 cut(s) 225, 234, 381, 522, 781
CfoI GCGC 4 cut(s) 23, 140, 220, 323
Cfr10I RCCGGY 5 cut(s) 223, 266, 272, 581, 779
Cfr13I GGNCC 6 cut(s) 252, 285, 286, 488, 594, 786
CseI GACGC 1 cut(s) 49
CsiI ACCWGGT 1 cut(s) 892
Csp6I GTAC 5 cut(s) 175, 368, 694, 910, 1097
CviAII CATG 5 cut(s) 277, 325, 521, 632, 726
CviQI GTAC 5 cut(s) 175, 368, 694, 910, 1097
DdeI CTNAG 4 cut(s) 155, 408, 549, 991
DinI GGCGCC 1 cut(s) 322
DpnI GATC 4 cut(s) 606, 720, 903, 1061
DpnII GATC 4 cut(s) 604, 718, 901, 1059
DrdI GACNNNNNNGTC 1 cut(s) 709
DseDI GACNNNNNNGTC 1 cut(s) 709
EaeI YGGCCR 4 cut(s) 225, 279, 327, 402
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 854
EarI CTCTTC 1 cut(s) 854
EciI GGCGGA 1 cut(s) 175
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 324, 441
Eco24I GRGCYC 1 cut(s) 289
Eco31I GGTCTC 1 cut(s) 883
Eco52I CGGCCG 1 cut(s) 402
Eco57I CTGAAG 2 cut(s) 527, 818
EcoO109I RGGNCCY 1 cut(s) 285
EcoRI GAATTC 1 cut(s) 636
EcoRII CCWGG 2 cut(s) 248, 892
EcoT14I CCWWGG 2 cut(s) 324, 441
EcoT38I GRGCYC 1 cut(s) 289
EgeI GGCGCC 1 cut(s) 322
EheI GGCGCC 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 324, 441
FaeI CATG 5 cut(s) 280, 328, 524, 635, 729
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 2 cut(s) 410, 568
FatI CATG 5 cut(s) 276, 324, 520, 631, 725
FauI CCCGC 3 cut(s) 85, 241, 413
FauNDI CATATG 1 cut(s) 1132
Fnu4HI GCNGC 3 cut(s) 94, 221, 405
FokI GGATG 1 cut(s) 252
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 3 cut(s) 94, 221, 405
FspBI CTAG 1 cut(s) 959
GlaI GCGC 4 cut(s) 22, 139, 219, 322
GluI GCNGC 3 cut(s) 94, 221, 405
GsaI CCCAGC 1 cut(s) 787
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 9 cut(s) 227, 254, 281, 287, 329, 404, 490, 596, 788
HgaI GACGC 1 cut(s) 49
HhaI GCGC 4 cut(s) 23, 140, 220, 323
Hin1I GRCGYC 2 cut(s) 321, 390
Hin1II CATG 5 cut(s) 280, 328, 524, 635, 729
Hin6I GCGC 4 cut(s) 21, 138, 218, 321
HinP1I GCGC 4 cut(s) 21, 138, 218, 321
HindIII AAGCTT 1 cut(s) 800
HinfI GANTC 5 cut(s) 61, 182, 410, 556, 955
HphI GGTGA 8 cut(s) 66, 457, 463, 478, 553, 580, 824, 844
Hpy166II GTNNAC 2 cut(s) 469, 1122
Hpy188I TCNGA 2 cut(s) 642, 839
Hpy188III TCNNGA 3 cut(s) 334, 700, 886
Hpy8I GTNNAC 2 cut(s) 469, 1122
Hpy99I CGWCG 2 cut(s) 43, 392
HpyAV CCTTC 8 cut(s) 118, 220, 349, 394, 442, 610, 801, 1101
HpyCH4III ACNGT 2 cut(s) 58, 865
HpyCH4IV ACGT 2 cut(s) 390, 474
HpyCH4V TGCA 5 cut(s) 533, 725, 860, 868, 878
HpyF3I CTNAG 4 cut(s) 155, 408, 549, 991
HpySE526I ACGT 2 cut(s) 390, 474
Hsp92I GRCGYC 2 cut(s) 321, 390
Hsp92II CATG 5 cut(s) 280, 328, 524, 635, 729
HspAI GCGC 4 cut(s) 21, 138, 218, 321
KasI GGCGCC 1 cut(s) 320
KroI GCCGGC 2 cut(s) 223, 779
KroNI GCCGGC 2 cut(s) 225, 781
Kzo9I GATC 4 cut(s) 604, 718, 901, 1059
LmnI GCTCC 5 cut(s) 101, 300, 356, 365, 599
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 1 cut(s) 520
MabI ACCWGGT 1 cut(s) 892
MaeI CTAG 1 cut(s) 959
MaeII ACGT 2 cut(s) 390, 474
MaeIII GTNAC 1 cut(s) 586
MalI GATC 4 cut(s) 606, 720, 903, 1061
MboI GATC 4 cut(s) 604, 718, 901, 1059
MboII GAAGA 6 cut(s) 3, 6, 418, 466, 533, 841
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 3 cut(s) 636, 840, 1035
Mly113I GGCGCC 1 cut(s) 321
MlyI GAGTC 3 cut(s) 176, 419, 550
MmeI TCCRAC 1 cut(s) 925
MnlI CCTC 9 cut(s) 109, 155, 164, 176, 308, 322, 325, 424, 857
MroNI GCCGGC 2 cut(s) 223, 779
MroXI GAANNNNTTC 1 cut(s) 980
MslI CAYNNNNRTG 2 cut(s) 275, 1046
MspA1I CMGCKG 1 cut(s) 407
MspR9I CCNGG 5 cut(s) 250, 283, 372, 592, 894
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 2 cut(s) 250, 894
MvnI CGCG 2 cut(s) 80, 218
NaeI GCCGGC 2 cut(s) 225, 781
NarI GGCGCC 1 cut(s) 321
NciI CCSGG 3 cut(s) 283, 372, 592
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 1132
NdeII GATC 4 cut(s) 604, 718, 901, 1059
NgoMIV GCCGGC 2 cut(s) 223, 779
NlaIII CATG 5 cut(s) 280, 328, 524, 635, 729
NlaIV GGNNCC 8 cut(s) 271, 286, 287, 322, 489, 595, 601, 720
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 1 cut(s) 524
PaeI GCATGC 1 cut(s) 524
PaqCI CACCTGC 2 cut(s) 454, 472
PcsI WCGNNNNNNNCGW 1 cut(s) 387
PctI GAATGC 1 cut(s) 649
PdiI GCCGGC 2 cut(s) 225, 781
PdmI GAANNNNTTC 1 cut(s) 980
PfeI GAWTC 2 cut(s) 61, 955
PflMI CCANNNNNTGG 2 cut(s) 34, 1136
PkrI GCNGC 3 cut(s) 95, 222, 406
PleI GAGTC 3 cut(s) 176, 418, 550
PluTI GGCGCC 1 cut(s) 324
PpsI GAGTC 3 cut(s) 176, 418, 550
Psp6I CCWGG 2 cut(s) 248, 892
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 2 cut(s) 248, 892
PspN4I GGNNCC 8 cut(s) 271, 286, 287, 322, 489, 595, 601, 720
PspOMI GGGCCC 1 cut(s) 285
PspPI GGNCC 6 cut(s) 252, 285, 286, 488, 594, 786
PsuI RGATCY 1 cut(s) 718
RsaI GTAC 5 cut(s) 176, 369, 695, 911, 1098
RsaNI GTAC 5 cut(s) 175, 368, 694, 910, 1097
RseI CAYNNNNRTG 2 cut(s) 275, 1046
SatI GCNGC 3 cut(s) 94, 221, 405
Sau3AI GATC 4 cut(s) 604, 718, 901, 1059
Sau96I GGNCC 6 cut(s) 252, 285, 286, 488, 594, 786
SchI GAGTC 3 cut(s) 176, 419, 550
ScrFI CCNGG 5 cut(s) 250, 283, 372, 592, 894
SduI GDGCHC 1 cut(s) 289
SexAI ACCWGGT 1 cut(s) 892
SfaNI GCATC 1 cut(s) 520
SfcI CTRYAG 1 cut(s) 355
SfoI GGCGCC 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 275, 1046
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
SphI GCATGC 1 cut(s) 524
Sse9I AATT 3 cut(s) 636, 840, 1035
SsiI CCGC 9 cut(s) 78, 186, 221, 234, 405, 420, 716, 829, 1026
SspDI GGCGCC 1 cut(s) 320
SspI AATATT 3 cut(s) 628, 979, 1080
SspMI CTAG 1 cut(s) 959
StyD4I CCNGG 5 cut(s) 248, 281, 370, 590, 892
StyI CCWWGG 2 cut(s) 324, 441
TaaI ACNGT 2 cut(s) 58, 865
TaiI ACGT 2 cut(s) 393, 477
TaqI TCGA 1 cut(s) 387
TasI AATT 3 cut(s) 636, 840, 1035
TatI WGTACW 2 cut(s) 909, 1096
TauI GCSGC 2 cut(s) 223, 407
TfiI GAWTC 2 cut(s) 61, 955
TscAI CASTG 3 cut(s) 57, 176, 678
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 1 cut(s) 93
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 202, 620
TspGWI ACGGA 2 cut(s) 484, 699
TspRI CASTG 3 cut(s) 57, 176, 678
Van91I CCANNNNNTGG 2 cut(s) 34, 1136
XapI RAATTY 1 cut(s) 636
XceI RCATGY 1 cut(s) 524
XcmI CCANNNNNNNNNTGG 2 cut(s) 941, 1048
XmnI GAANNNNTTC 1 cut(s) 980
XspI CTAG 1 cut(s) 959
ZraI GACGTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.