Rmu_ssc0000192.1_g000014

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000192.1
OG0000931
Physical Location & Seq
Reverse (-)
88043 .. 90566
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000192.1_g000014.1.cds

Sequence Viewer

Length: 1749 bp
atggggccgggttcggggagaaaggtcatcaatttagagggaggtgatggtgaatgggatgagaggactgagaggcgtttgaggagaaggggtatagctaatggaatttacaaccccaatttgcgtcgcagtacccctaagaaaagcagaaagtgtaatgcacaagtggagaacctaatgttggataaggattatgagaaatacctgagtttgattcagcggggtcgggacttgaatgaacattctagtgatcacggtattaatggggattgcgagaatgaacataatgatgatgggaatgaacatcgtactggtgaaaataatgatattaggtctgtggacggtgatggaaacaagcattctgttgatgacaataatgatgccagggttgtggatggtagtgggagcgaacattctgttgatgatactgtcaacttgaagtctgtggatcacagtggtggtaggaatggacgatgtggcgtggatgatggtgatggtgggaatgctcattttggtgatggcaacattcatgttaattctttgaatcatggcgtcgatgatgatgatatggatcctcagtataagatattctgggacaatttgaaggaagatggaaattcatacgtactggagatggttcaagaggatggacgtgtgaaacagataaggtatcaccaaggcgatggtttacctgatgagctcgatctagatgccacgcattcccagggaaaacagaagactgaatttaaaaacagggagactatggaaaatttagaggatgccccagagaaaaggttttcagtgaggaagaggaatgtaaaattggaagctctggaacctattttagacggaacaaatgtgtgttctagtaaacgacctcgtttagaggttccaaacactccaaaaagtttaggaagttctccacagaaagagaaaactgaaactagaagaaattcaaggagtgtcattaagaagagaaatttagaggatcgtccggcgaaaaagaatggtcttgagaaaaagagttcaatgttgaagaagaatgtgaaggaagcagatgcagatcctctttctggtagagcaaatgggcgttctcataaacagtctggttcaaaatatctttggttctcagagggtgggtgcaatagtgaagctgaatctgattctaatctaacagatgacagctatcaagagttcttaagttgtactaaggatgatgggaaagttatggtatttacatccagaatttgtggaccattaatatatgattcagatgaagaaagttcatcagattcggatgtgatcgtgctcgacagggatccacatgagggataccgtacaccaattattgatgtagacagtcggcggtccactgaaagtcattcccagtttaggcaagggattatgcaggatcttaaaaggccttaccaccaagaagagtatatcaatctcttggaagactggtcttgccaaaggccagccacaggtcatgatagaaatctacgaaatggaaatgtaaaatcatatgcactgccgggggaatttgggaaatcatatcaagagatgtacaaagatctggcgggaaagattaatgcagtgacaggcgatgatcgccctagaaagttaaatcttttgcgtggatttttctactggttgaagaatatttcccaggaaggaagctttaaaccttggaatgacaaagcatgtttgaaggtggtgccgcagacactaaataggaggacttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

582

Amino Acids

65.81

Weight (kDa)

5.87

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1717
AccI GTMKAC 1 cut(s) 1356
AciI CCGC 4 cut(s) 220, 1366, 1580, 1721
AclWI GGATC 8 cut(s) 456, 566, 579, 996, 1058, 1313, 1326, 1419
AcsI RAATTY 8 cut(s) 105, 616, 743, 769, 952, 979, 1245, 1541
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 6 cut(s) 133, 310, 627, 1207, 1339, 1568
AfiI CCNNNNNNNGG 6 cut(s) 14, 181, 1073, 1328, 1392, 1484
AflII CTTAAG 1 cut(s) 1198
AflIII ACRYGT 1 cut(s) 652
AjiI CACGTC 1 cut(s) 653
AjnI CCWGG 3 cut(s) 383, 723, 1668
AjuI GAANNNNNNNTTGG 4 cut(s) 164, 196, 806, 838
AleI CACNNNNGTG 1 cut(s) 456
AluBI AGCT 6 cut(s) 98, 700, 830, 1154, 1185, 1680
AluI AGCT 6 cut(s) 98, 700, 830, 1154, 1185, 1680
Alw21I GWGCWC 2 cut(s) 702, 1311
Alw26I GTCTC 1 cut(s) 752
AlwI GGATC 8 cut(s) 456, 566, 579, 996, 1058, 1313, 1326, 1419
AoxI GGCC 3 cut(s) 5, 1421, 1475
ApoI RAATTY 8 cut(s) 105, 616, 743, 769, 952, 979, 1245, 1541
AseI ATTAAT 3 cut(s) 261, 1259, 1590
Asp700I GAANNNNTTC 1 cut(s) 952
AspS9I GGNCC 3 cut(s) 5, 1253, 1368
AsuC2I CCSGG 2 cut(s) 9, 1536
AsuHPI GGTGA 7 cut(s) 56, 62, 326, 356, 503, 527, 665
AvaII GGWCC 2 cut(s) 1253, 1368
BamHI GGATCC 2 cut(s) 571, 1318
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 1 cut(s) 702
BbsI GAAGAC 2 cut(s) 743, 1464
Bbv12I GWGCWC 2 cut(s) 702, 1311
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BciT130I CCWGG 3 cut(s) 385, 725, 1670
BciVI GTATCC 1 cut(s) 1325
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 2 cut(s) 9, 1536
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 5 cut(s) 246, 707, 867, 945, 1617
BfrI CTTAAG 1 cut(s) 1198
BfuI GTATCC 1 cut(s) 1325
BglII AGATCT 1 cut(s) 1573
BisI GCNGC 1 cut(s) 1721
BlsI GCNGC 1 cut(s) 1722
Bme1390I CCNGG 5 cut(s) 9, 385, 725, 1536, 1670
Bme18I GGWCC 2 cut(s) 1253, 1368
BmgBI CACGTC 1 cut(s) 653
BmgT120I GGNCC 3 cut(s) 5, 1253, 1368
BmiI GGNNCC 6 cut(s) 6, 573, 837, 891, 1320, 1719
BmrFI CCNGG 5 cut(s) 9, 385, 725, 1536, 1670
BmrI ACTGGG 1 cut(s) 1381
BmsI GCATC 4 cut(s) 370, 700, 769, 1048
BmuI ACTGGG 1 cut(s) 1381
BpiI GAAGAC 2 cut(s) 743, 1464
BpmI CTGGAG 1 cut(s) 650
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 2 cut(s) 9, 1536
BsaAI YACGTR 1 cut(s) 625
BsaBI GATNNNNATC 4 cut(s) 570, 1062, 1167, 1497
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 7 cut(s) 384, 676, 723, 724, 1535, 1668, 1688
BsaXI ACNNNNNCTCC 2 cut(s) 76, 106
Bsc4I CCNNNNNNNGG 6 cut(s) 14, 181, 1073, 1328, 1392, 1484
Bse1I ACTGG 5 cut(s) 316, 633, 1387, 1466, 1655
Bse8I GATNNNNATC 4 cut(s) 570, 1062, 1167, 1497
BseBI CCWGG 3 cut(s) 385, 725, 1670
BseDI CCNNGG 7 cut(s) 384, 676, 723, 724, 1535, 1668, 1688
BseGI GGATG 8 cut(s) 64, 400, 490, 652, 784, 1219, 1238, 1303
BseJI GATNNNNATC 4 cut(s) 570, 1062, 1167, 1497
BseLI CCNNNNNNNGG 6 cut(s) 14, 181, 1073, 1328, 1392, 1484
BseMII CTCAG 4 cut(s) 60, 197, 590, 1143
BseNI ACTGG 5 cut(s) 316, 633, 1387, 1466, 1655
BseRI GAGGAG 1 cut(s) 97
BshFI GGCC 3 cut(s) 7, 1423, 1477
BshNI GGYRCC 1 cut(s) 1717
BsiHKAI GWGCWC 2 cut(s) 702, 1311
BsiSI CCGG 3 cut(s) 8, 995, 1535
BslFI GGGAC 2 cut(s) 242, 608
BslI CCNNNNNNNGG 6 cut(s) 14, 181, 1073, 1328, 1392, 1484
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 242, 608
BsmI GAATGC 3 cut(s) 358, 508, 718
BsnI GGCC 3 cut(s) 7, 1423, 1477
Bsp1286I GDGCHC 2 cut(s) 702, 1311
Bsp1407I TGTACA 1 cut(s) 1566
BspACI CCGC 4 cut(s) 220, 1366, 1580, 1721
BspANI GGCC 3 cut(s) 7, 1423, 1477
BspCNI CTCAG 4 cut(s) 61, 198, 589, 1142
BspHI TCATGA 1 cut(s) 1489
BspLI GGNNCC 6 cut(s) 6, 573, 837, 891, 1320, 1719
BspPI GGATC 8 cut(s) 456, 566, 579, 996, 1058, 1313, 1326, 1419
BspT107I GGYRCC 1 cut(s) 1717
BspTI CTTAAG 1 cut(s) 1198
BsrGI TGTACA 1 cut(s) 1566
BsrI ACTGG 5 cut(s) 316, 633, 1387, 1466, 1655
BssECI CCNNGG 7 cut(s) 384, 676, 723, 724, 1535, 1668, 1688
BssNI GRCGYC 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 676, 1688
Bst2UI CCWGG 3 cut(s) 385, 725, 1670
Bst4CI ACNGT 7 cut(s) 257, 344, 430, 455, 1104, 1337, 1361
Bst6I CTCTTC 3 cut(s) 803, 968, 1431
BstACI GRCGYC 1 cut(s) 552
BstAFI CTTAAG 1 cut(s) 1198
BstAUI TGTACA 1 cut(s) 1566
BstBAI YACGTR 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 1479
BstDEI CTNAG 6 cut(s) 69, 138, 206, 576, 1129, 1209
BstF5I GGATG 8 cut(s) 64, 400, 490, 652, 784, 1219, 1238, 1303
BstMAI GTCTC 1 cut(s) 752
BstMWI GCNNNNNNNGC 1 cut(s) 1611
BstNI CCWGG 3 cut(s) 385, 725, 1670
BstNSI RCATGY 1 cut(s) 1707
BstSCI CCNGG 5 cut(s) 7, 383, 723, 1534, 1668
BstSNI TACGTA 1 cut(s) 625
BstV2I GAAGAC 2 cut(s) 743, 1464
BstX2I RGATCY 5 cut(s) 571, 1063, 1318, 1411, 1573
BstXI CCANNNNNNTGG 2 cut(s) 391, 683
BstYI RGATCY 5 cut(s) 571, 1063, 1318, 1411, 1573
BsuI GTATCC 1 cut(s) 1325
BsuRI GGCC 3 cut(s) 7, 1423, 1477
BtgZI GCGATG 2 cut(s) 696, 1620
BtrI CACGTC 1 cut(s) 653
BtsCI GGATG 8 cut(s) 64, 400, 490, 652, 784, 1219, 1238, 1303
BtsI GCAGTG 2 cut(s) 1529, 1602
BtsIMutI CAGTG 5 cut(s) 460, 807, 1371, 1529, 1602
Cac8I GCNNGC 1 cut(s) 1479
CciI TCATGA 1 cut(s) 1489
Cfr13I GGNCC 3 cut(s) 5, 1253, 1368
CseI GACGC 2 cut(s) 113, 541
Csp6I GTAC 6 cut(s) 132, 309, 626, 1206, 1338, 1567
CviAII CATG 5 cut(s) 530, 548, 1325, 1490, 1704
CviQI GTAC 6 cut(s) 132, 309, 626, 1206, 1338, 1567
DdeI CTNAG 6 cut(s) 69, 138, 206, 576, 1129, 1209
DraI TTTAAA 2 cut(s) 748, 1684
Eam1104I CTCTTC 3 cut(s) 803, 968, 1431
EarI CTCTTC 3 cut(s) 803, 968, 1431
Ecl136II GAGCTC 1 cut(s) 700
Eco105I TACGTA 1 cut(s) 625
Eco130I CCWWGG 2 cut(s) 676, 1688
Eco147I AGGCCT 1 cut(s) 1423
Eco24I GRGCYC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 1253, 1368
Eco53kI GAGCTC 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 700
EcoRII CCWGG 3 cut(s) 383, 723, 1668
EcoT14I CCWWGG 2 cut(s) 676, 1688
EcoT38I GRGCYC 1 cut(s) 702
ErhI CCWWGG 2 cut(s) 676, 1688
FaeI CATG 5 cut(s) 533, 551, 1328, 1493, 1707
FaqI GGGAC 2 cut(s) 242, 608
FatI CATG 5 cut(s) 529, 547, 1324, 1489, 1703
FauI CCCGC 2 cut(s) 213, 1573
FauNDI CATATG 1 cut(s) 1525
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 1356
Fnu4HI GCNGC 1 cut(s) 1721
FokI GGATG 8 cut(s) 71, 407, 497, 659, 791, 1225, 1226, 1310
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 1 cut(s) 1721
FspBI CTAG 5 cut(s) 246, 707, 867, 945, 1617
GluI GCNGC 1 cut(s) 1721
GsuI CTGGAG 1 cut(s) 650
HaeIII GGCC 3 cut(s) 7, 1423, 1477
HapII CCGG 3 cut(s) 8, 995, 1535
HgaI GACGC 2 cut(s) 113, 541
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 5 cut(s) 533, 551, 1328, 1493, 1707
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 1678
HinfI GANTC 6 cut(s) 214, 544, 1157, 1163, 1268, 1292
HpaII CCGG 3 cut(s) 8, 995, 1535
HphI GGTGA 7 cut(s) 56, 62, 326, 356, 503, 527, 665
Hpy166II GTNNAC 8 cut(s) 340, 433, 689, 872, 1253, 1340, 1357, 1371
Hpy188I TCNGA 5 cut(s) 1132, 1162, 1273, 1291, 1297
Hpy8I GTNNAC 8 cut(s) 340, 433, 689, 872, 1253, 1340, 1357, 1371
Hpy99I CGWCG 2 cut(s) 129, 557
HpyAV CCTTC 5 cut(s) 81, 598, 1042, 1667, 1705
HpyCH4III ACNGT 7 cut(s) 257, 344, 430, 455, 1104, 1337, 1361
HpyCH4IV ACGT 2 cut(s) 624, 652
HpyCH4V TGCA 6 cut(s) 161, 1061, 1143, 1408, 1529, 1595
HpyF10VI GCNNNNNNNGC 1 cut(s) 1611
HpyF3I CTNAG 6 cut(s) 69, 138, 206, 576, 1129, 1209
HpySE526I ACGT 2 cut(s) 624, 652
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 5 cut(s) 533, 551, 1328, 1493, 1707
Ksp22I TGATCA 1 cut(s) 250
LmnI GCTCC 1 cut(s) 405
LweI GCATC 4 cut(s) 370, 700, 769, 1048
MaeI CTAG 5 cut(s) 246, 707, 867, 945, 1617
MaeII ACGT 2 cut(s) 624, 652
MaeIII GTNAC 1 cut(s) 1597
MflI RGATCY 5 cut(s) 571, 1063, 1318, 1411, 1573
MhlI GDGCHC 2 cut(s) 702, 1311
MmeI TCCRAC 1 cut(s) 162
MroXI GAANNNNTTC 1 cut(s) 952
MslI CAYNNNNRTG 4 cut(s) 246, 288, 456, 513
MspA1I CMGCKG 1 cut(s) 220
MspCI CTTAAG 1 cut(s) 1198
MspI CCGG 3 cut(s) 8, 995, 1535
MspR9I CCNGG 5 cut(s) 9, 385, 725, 1536, 1670
Mva1269I GAATGC 3 cut(s) 358, 508, 718
MvaI CCWGG 3 cut(s) 385, 725, 1670
MwoI GCNNNNNNNGC 1 cut(s) 1611
NciI CCSGG 2 cut(s) 9, 1536
NdeI CATATG 1 cut(s) 1525
NlaIII CATG 5 cut(s) 533, 551, 1328, 1493, 1707
NlaIV GGNNCC 6 cut(s) 6, 573, 837, 891, 1320, 1719
NmuCI GTSAC 1 cut(s) 1597
NspI RCATGY 1 cut(s) 1707
OliI CACNNNNGTG 1 cut(s) 456
PagI TCATGA 1 cut(s) 1489
PasI CCCWGGG 1 cut(s) 724
PceI AGGCCT 1 cut(s) 1423
PctI GAATGC 3 cut(s) 358, 508, 718
PdmI GAANNNNTTC 1 cut(s) 952
PfeI GAWTC 6 cut(s) 214, 544, 1157, 1163, 1268, 1292
PkrI GCNGC 1 cut(s) 1722
Ppu21I YACGTR 1 cut(s) 625
PshBI ATTAAT 3 cut(s) 261, 1259, 1590
Psp124BI GAGCTC 1 cut(s) 702
Psp6I CCWGG 3 cut(s) 383, 723, 1668
PspGI CCWGG 3 cut(s) 383, 723, 1668
PspN4I GGNNCC 6 cut(s) 6, 573, 837, 891, 1320, 1719
PspPI GGNCC 3 cut(s) 5, 1253, 1368
PsuI RGATCY 5 cut(s) 571, 1063, 1318, 1411, 1573
RsaI GTAC 6 cut(s) 133, 310, 627, 1207, 1339, 1568
RsaNI GTAC 6 cut(s) 132, 309, 626, 1206, 1338, 1567
RseI CAYNNNNRTG 4 cut(s) 246, 288, 456, 513
SacI GAGCTC 1 cut(s) 702
SatI GCNGC 1 cut(s) 1721
Sau96I GGNCC 3 cut(s) 5, 1253, 1368
ScrFI CCNGG 5 cut(s) 9, 385, 725, 1536, 1670
SduI GDGCHC 2 cut(s) 702, 1311
SfaNI GCATC 4 cut(s) 370, 700, 769, 1048
SinI GGWCC 2 cut(s) 1253, 1368
SmiMI CAYNNNNRTG 4 cut(s) 246, 288, 456, 513
SmlI CTYRAG 2 cut(s) 1013, 1198
SmoI CTYRAG 2 cut(s) 1013, 1198
SnaBI TACGTA 1 cut(s) 625
SseBI AGGCCT 1 cut(s) 1423
SsiI CCGC 4 cut(s) 220, 1366, 1580, 1721
SspI AATATT 1 cut(s) 1663
SspMI CTAG 5 cut(s) 246, 707, 867, 945, 1617
SstI GAGCTC 1 cut(s) 702
StuI AGGCCT 1 cut(s) 1423
StyD4I CCNGG 5 cut(s) 7, 383, 723, 1534, 1668
StyI CCWWGG 2 cut(s) 676, 1688
TaaI ACNGT 7 cut(s) 257, 344, 430, 455, 1104, 1337, 1361
TaiI ACGT 2 cut(s) 627, 655
TaqI TCGA 3 cut(s) 555, 702, 1311
TatI WGTACW 2 cut(s) 1205, 1566
TauI GCSGC 1 cut(s) 1723
TfiI GAWTC 6 cut(s) 214, 544, 1157, 1163, 1268, 1292
TscAI CASTG 5 cut(s) 460, 807, 1378, 1536, 1602
TseFI GTSAC 1 cut(s) 1597
Tsp45I GTSAC 1 cut(s) 1597
TspDTI ATGAA 7 cut(s) 252, 294, 315, 518, 609, 1275, 1290
TspGWI ACGGA 1 cut(s) 864
TspRI CASTG 5 cut(s) 460, 807, 1378, 1536, 1602
Vha464I CTTAAG 1 cut(s) 1198
VpaK11BI GGWCC 2 cut(s) 1253, 1368
VspI ATTAAT 3 cut(s) 261, 1259, 1590
XapI RAATTY 8 cut(s) 105, 616, 743, 769, 952, 979, 1245, 1541
XbaI TCTAGA 1 cut(s) 706
XceI RCATGY 1 cut(s) 1707
XmiI GTMKAC 1 cut(s) 1356
XmnI GAANNNNTTC 1 cut(s) 952
XspI CTAG 5 cut(s) 246, 707, 867, 945, 1617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.