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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1043 |
| Acc36I | ACCTGC | 1 cut(s) | 1043 |
| AccB1I | GGYRCC | 1 cut(s) | 1717 |
| AccI | GTMKAC | 1 cut(s) | 1356 |
| AciI | CCGC | 3 cut(s) | 217, 1366, 1721 |
| AclWI | GGATC | 7 cut(s) | 453, 563, 993, 1055, 1310, 1323, 1419 |
| AfaI | GTAC | 4 cut(s) | 130, 307, 1204, 1568 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 14, 178, 1070, 1325, 1392, 1484 |
| AflII | CTTAAG | 1 cut(s) | 1195 |
| AflIII | ACRYGT | 1 cut(s) | 649 |
| AjiI | CACGTC | 1 cut(s) | 650 |
| AjnI | CCWGG | 2 cut(s) | 380, 1668 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 161, 193, 803, 835 |
| AleI | CACNNNNGTG | 2 cut(s) | 453, 675 |
| AluBI | AGCT | 5 cut(s) | 95, 697, 1151, 1182, 1680 |
| AluI | AGCT | 5 cut(s) | 95, 697, 1151, 1182, 1680 |
| Alw21I | GWGCWC | 2 cut(s) | 505, 1308 |
| Alw26I | GTCTC | 3 cut(s) | 749, 1470, 1556 |
| AlwI | GGATC | 7 cut(s) | 453, 563, 993, 1055, 1310, 1323, 1419 |
| AoxI | GGCC | 3 cut(s) | 5, 1421, 1475 |
| AseI | ATTAAT | 2 cut(s) | 258, 1256 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 949 |
| AspS9I | GGNCC | 3 cut(s) | 5, 1250, 1368 |
| AsuC2I | CCSGG | 2 cut(s) | 9, 1536 |
| AsuHPI | GGTGA | 8 cut(s) | 53, 59, 323, 353, 500, 524, 662, 689 |
| AvaII | GGWCC | 2 cut(s) | 1250, 1368 |
| BamHI | GGATCC | 1 cut(s) | 1315 |
| BanI | GGYRCC | 1 cut(s) | 1717 |
| BbsI | GAAGAC | 2 cut(s) | 740, 1464 |
| Bbv12I | GWGCWC | 2 cut(s) | 505, 1308 |
| BceAI | ACGGC | 1 cut(s) | 533 |
| BciT130I | CCWGG | 2 cut(s) | 382, 1670 |
| BciVI | GTATCC | 1 cut(s) | 1322 |
| BclI | TGATCA | 1 cut(s) | 247 |
| BcnI | CCSGG | 2 cut(s) | 9, 1536 |
| BcoDI | GTCTC | 3 cut(s) | 749, 1470, 1556 |
| BfaI | CTAG | 6 cut(s) | 243, 704, 864, 942, 1433, 1617 |
| BfmI | CTRYAG | 1 cut(s) | 920 |
| BfrI | CTTAAG | 1 cut(s) | 1195 |
| BfuAI | ACCTGC | 1 cut(s) | 1043 |
| BfuI | GTATCC | 1 cut(s) | 1322 |
| BglII | AGATCT | 1 cut(s) | 1573 |
| BisI | GCNGC | 1 cut(s) | 1721 |
| BlsI | GCNGC | 1 cut(s) | 1722 |
| Bme1390I | CCNGG | 4 cut(s) | 9, 382, 1536, 1670 |
| Bme18I | GGWCC | 2 cut(s) | 1250, 1368 |
| BmgBI | CACGTC | 1 cut(s) | 650 |
| BmgT120I | GGNCC | 3 cut(s) | 5, 1250, 1368 |
| BmiI | GGNNCC | 5 cut(s) | 6, 834, 888, 1317, 1719 |
| BmrFI | CCNGG | 4 cut(s) | 9, 382, 1536, 1670 |
| BmrI | ACTGGG | 1 cut(s) | 1381 |
| BmsI | GCATC | 4 cut(s) | 367, 697, 766, 1582 |
| BmuI | ACTGGG | 1 cut(s) | 1381 |
| BpiI | GAAGAC | 2 cut(s) | 740, 1464 |
| BpmI | CTGGAG | 1 cut(s) | 647 |
| BpuEI | CTTGAG | 1 cut(s) | 1031 |
| BpuMI | CCSGG | 2 cut(s) | 9, 1536 |
| BsaBI | GATNNNNATC | 3 cut(s) | 567, 1164, 1497 |
| BsaI | GGTCTC | 1 cut(s) | 1470 |
| BsaJI | CCNNGG | 5 cut(s) | 381, 673, 1535, 1668, 1688 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 73, 103 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 14, 178, 1070, 1325, 1392, 1484 |
| Bse1I | ACTGG | 4 cut(s) | 313, 1387, 1466, 1655 |
| Bse8I | GATNNNNATC | 3 cut(s) | 567, 1164, 1497 |
| BseBI | CCWGG | 2 cut(s) | 382, 1670 |
| BseDI | CCNNGG | 5 cut(s) | 381, 673, 1535, 1668, 1688 |
| BseGI | GGATG | 6 cut(s) | 397, 487, 649, 781, 1216, 1235 |
| BseJI | GATNNNNATC | 3 cut(s) | 567, 1164, 1497 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 14, 178, 1070, 1325, 1392, 1484 |
| BseMII | CTCAG | 5 cut(s) | 57, 194, 587, 734, 1140 |
| BseNI | ACTGG | 4 cut(s) | 313, 1387, 1466, 1655 |
| BseRI | GAGGAG | 1 cut(s) | 94 |
| BsgI | GTGCAG | 1 cut(s) | 1077 |
| BshFI | GGCC | 3 cut(s) | 7, 1423, 1477 |
| BshNI | GGYRCC | 1 cut(s) | 1717 |
| BsiHKAI | GWGCWC | 2 cut(s) | 505, 1308 |
| BsiSI | CCGG | 2 cut(s) | 8, 1535 |
| BslFI | GGGAC | 2 cut(s) | 239, 605 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 14, 178, 1070, 1325, 1392, 1484 |
| BsmAI | GTCTC | 3 cut(s) | 749, 1470, 1556 |
| BsmBI | CGTCTC | 1 cut(s) | 1556 |
| BsmFI | GGGAC | 2 cut(s) | 239, 605 |
| BsmI | GAATGC | 2 cut(s) | 355, 715 |
| BsnI | GGCC | 3 cut(s) | 7, 1423, 1477 |
| Bso31I | GGTCTC | 1 cut(s) | 1470 |
| Bsp1286I | GDGCHC | 2 cut(s) | 505, 1308 |
| BspACI | CCGC | 3 cut(s) | 217, 1366, 1721 |
| BspANI | GGCC | 3 cut(s) | 7, 1423, 1477 |
| BspCNI | CTCAG | 5 cut(s) | 58, 195, 586, 733, 1139 |
| BspHI | TCATGA | 1 cut(s) | 1489 |
| BspLI | GGNNCC | 5 cut(s) | 6, 834, 888, 1317, 1719 |
| BspMAI | CTGCAG | 1 cut(s) | 924 |
| BspMI | ACCTGC | 1 cut(s) | 1043 |
| BspPI | GGATC | 7 cut(s) | 453, 563, 993, 1055, 1310, 1323, 1419 |
| BspT107I | GGYRCC | 1 cut(s) | 1717 |
| BspTI | CTTAAG | 1 cut(s) | 1195 |
| BspTNI | GGTCTC | 1 cut(s) | 1470 |
| BsrI | ACTGG | 4 cut(s) | 313, 1387, 1466, 1655 |
| BssECI | CCNNGG | 5 cut(s) | 381, 673, 1535, 1668, 1688 |
| BssT1I | CCWWGG | 2 cut(s) | 673, 1688 |
| Bst2UI | CCWGG | 2 cut(s) | 382, 1670 |
| Bst4CI | ACNGT | 5 cut(s) | 254, 341, 427, 452, 1361 |
| Bst6I | CTCTTC | 2 cut(s) | 965, 1431 |
| BstAFI | CTTAAG | 1 cut(s) | 1195 |
| BstC8I | GCNNGC | 1 cut(s) | 1479 |
| BstDEI | CTNAG | 6 cut(s) | 66, 203, 573, 720, 1126, 1206 |
| BstF5I | GGATG | 6 cut(s) | 397, 487, 649, 781, 1216, 1235 |
| BstMAI | GTCTC | 3 cut(s) | 749, 1470, 1556 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1611 |
| BstNI | CCWGG | 2 cut(s) | 382, 1670 |
| BstNSI | RCATGY | 1 cut(s) | 1707 |
| BstSCI | CCNGG | 4 cut(s) | 7, 380, 1534, 1668 |
| BstSFI | CTRYAG | 1 cut(s) | 920 |
| BstV2I | GAAGAC | 2 cut(s) | 740, 1464 |
| BstX2I | RGATCY | 4 cut(s) | 1060, 1315, 1411, 1573 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 388, 680 |
| BstYI | RGATCY | 4 cut(s) | 1060, 1315, 1411, 1573 |
| BsuI | GTATCC | 1 cut(s) | 1322 |
| BsuRI | GGCC | 3 cut(s) | 7, 1423, 1477 |
| BtgZI | GCGATG | 1 cut(s) | 1620 |
| BtrI | CACGTC | 1 cut(s) | 650 |
| BtsCI | GGATG | 6 cut(s) | 397, 487, 649, 781, 1216, 1235 |
| BtsI | GCAGTG | 2 cut(s) | 1529, 1602 |
| BtsIMutI | CAGTG | 4 cut(s) | 804, 1371, 1529, 1602 |
| BveI | ACCTGC | 1 cut(s) | 1043 |
| Cac8I | GCNNGC | 1 cut(s) | 1479 |
| CciI | TCATGA | 1 cut(s) | 1489 |
| Cfr13I | GGNCC | 3 cut(s) | 5, 1250, 1368 |
| CseI | GACGC | 1 cut(s) | 110 |
| Csp6I | GTAC | 4 cut(s) | 129, 306, 1203, 1567 |
| CviAII | CATG | 5 cut(s) | 527, 545, 1322, 1490, 1704 |
| CviQI | GTAC | 4 cut(s) | 129, 306, 1203, 1567 |
| DdeI | CTNAG | 6 cut(s) | 66, 203, 573, 720, 1126, 1206 |
| DraI | TTTAAA | 2 cut(s) | 745, 1684 |
| Eam1104I | CTCTTC | 2 cut(s) | 965, 1431 |
| EarI | CTCTTC | 2 cut(s) | 965, 1431 |
| Eco130I | CCWWGG | 2 cut(s) | 673, 1688 |
| Eco147I | AGGCCT | 1 cut(s) | 1423 |
| Eco31I | GGTCTC | 1 cut(s) | 1470 |
| Eco47I | GGWCC | 2 cut(s) | 1250, 1368 |
| EcoRI | GAATTC | 1 cut(s) | 50 |
| EcoRII | CCWGG | 2 cut(s) | 380, 1668 |
| EcoT14I | CCWWGG | 2 cut(s) | 673, 1688 |
| ErhI | CCWWGG | 2 cut(s) | 673, 1688 |
| Esp3I | CGTCTC | 1 cut(s) | 1556 |
| FaeI | CATG | 5 cut(s) | 530, 548, 1325, 1493, 1707 |
| FaqI | GGGAC | 2 cut(s) | 239, 605 |
| FatI | CATG | 5 cut(s) | 526, 544, 1321, 1489, 1703 |
| FauI | CCCGC | 1 cut(s) | 210 |
| FauNDI | CATATG | 1 cut(s) | 1525 |
| FbaI | TGATCA | 1 cut(s) | 247 |
| FblI | GTMKAC | 1 cut(s) | 1356 |
| Fnu4HI | GCNGC | 1 cut(s) | 1721 |
| FokI | GGATG | 6 cut(s) | 404, 494, 656, 788, 1222, 1223 |
| Fsp4HI | GCNGC | 1 cut(s) | 1721 |
| FspBI | CTAG | 6 cut(s) | 243, 704, 864, 942, 1433, 1617 |
| GluI | GCNGC | 1 cut(s) | 1721 |
| GsuI | CTGGAG | 1 cut(s) | 647 |
| HaeIII | GGCC | 3 cut(s) | 7, 1423, 1477 |
| HapII | CCGG | 2 cut(s) | 8, 1535 |
| HgaI | GACGC | 1 cut(s) | 110 |
| Hin1II | CATG | 5 cut(s) | 530, 548, 1325, 1493, 1707 |
| HincII | GTYRAC | 1 cut(s) | 430 |
| HindII | GTYRAC | 1 cut(s) | 430 |
| HindIII | AAGCTT | 1 cut(s) | 1678 |
| HinfI | GANTC | 6 cut(s) | 211, 541, 1154, 1160, 1265, 1289 |
| HpaII | CCGG | 2 cut(s) | 8, 1535 |
| HphI | GGTGA | 8 cut(s) | 53, 59, 323, 353, 500, 524, 662, 689 |
| Hpy166II | GTNNAC | 6 cut(s) | 337, 430, 686, 869, 1357, 1371 |
| Hpy188I | TCNGA | 6 cut(s) | 844, 1129, 1159, 1270, 1288, 1294 |
| Hpy8I | GTNNAC | 6 cut(s) | 337, 430, 686, 869, 1357, 1371 |
| Hpy99I | CGWCG | 1 cut(s) | 126 |
| HpyAV | CCTTC | 5 cut(s) | 78, 595, 1039, 1667, 1705 |
| HpyCH4III | ACNGT | 5 cut(s) | 254, 341, 427, 452, 1361 |
| HpyCH4IV | ACGT | 3 cut(s) | 621, 649, 1565 |
| HpyCH4V | TGCA | 7 cut(s) | 158, 922, 1058, 1140, 1408, 1529, 1595 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1611 |
| HpyF3I | CTNAG | 6 cut(s) | 66, 203, 573, 720, 1126, 1206 |
| HpySE526I | ACGT | 3 cut(s) | 621, 649, 1565 |
| Hsp92II | CATG | 5 cut(s) | 530, 548, 1325, 1493, 1707 |
| Ksp22I | TGATCA | 1 cut(s) | 247 |
| LmnI | GCTCC | 1 cut(s) | 402 |
| LweI | GCATC | 4 cut(s) | 367, 697, 766, 1582 |
| MaeI | CTAG | 6 cut(s) | 243, 704, 864, 942, 1433, 1617 |
| MaeII | ACGT | 3 cut(s) | 621, 649, 1565 |
| MaeIII | GTNAC | 1 cut(s) | 1597 |
| MflI | RGATCY | 4 cut(s) | 1060, 1315, 1411, 1573 |
| MhlI | GDGCHC | 2 cut(s) | 505, 1308 |
| MmeI | TCCRAC | 1 cut(s) | 159 |
| MroXI | GAANNNNTTC | 1 cut(s) | 949 |
| MslI | CAYNNNNRTG | 5 cut(s) | 243, 285, 453, 510, 675 |
| MspA1I | CMGCKG | 1 cut(s) | 217 |
| MspCI | CTTAAG | 1 cut(s) | 1195 |
| MspI | CCGG | 2 cut(s) | 8, 1535 |
| MspR9I | CCNGG | 4 cut(s) | 9, 382, 1536, 1670 |
| Mva1269I | GAATGC | 2 cut(s) | 355, 715 |
| MvaI | CCWGG | 2 cut(s) | 382, 1670 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1611 |
| NciI | CCSGG | 2 cut(s) | 9, 1536 |
| NdeI | CATATG | 1 cut(s) | 1525 |
| NlaIII | CATG | 5 cut(s) | 530, 548, 1325, 1493, 1707 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 834, 888, 1317, 1719 |
| NmuCI | GTSAC | 1 cut(s) | 1597 |
| NspI | RCATGY | 1 cut(s) | 1707 |
| OliI | CACNNNNGTG | 2 cut(s) | 453, 675 |
| PagI | TCATGA | 1 cut(s) | 1489 |
| PaqCI | CACCTGC | 1 cut(s) | 1043 |
| PceI | AGGCCT | 1 cut(s) | 1423 |
| PctI | GAATGC | 2 cut(s) | 355, 715 |
| PdmI | GAANNNNTTC | 1 cut(s) | 949 |
| PfeI | GAWTC | 6 cut(s) | 211, 541, 1154, 1160, 1265, 1289 |
| PkrI | GCNGC | 1 cut(s) | 1722 |
| PshBI | ATTAAT | 2 cut(s) | 258, 1256 |
| Psp6I | CCWGG | 2 cut(s) | 380, 1668 |
| PspGI | CCWGG | 2 cut(s) | 380, 1668 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 834, 888, 1317, 1719 |
| PspPI | GGNCC | 3 cut(s) | 5, 1250, 1368 |
| PstI | CTGCAG | 1 cut(s) | 924 |
| PsuI | RGATCY | 4 cut(s) | 1060, 1315, 1411, 1573 |
| RsaI | GTAC | 4 cut(s) | 130, 307, 1204, 1568 |
| RsaNI | GTAC | 4 cut(s) | 129, 306, 1203, 1567 |
| RseI | CAYNNNNRTG | 5 cut(s) | 243, 285, 453, 510, 675 |
| SatI | GCNGC | 1 cut(s) | 1721 |
| Sau96I | GGNCC | 3 cut(s) | 5, 1250, 1368 |
| ScrFI | CCNGG | 4 cut(s) | 9, 382, 1536, 1670 |
| SduI | GDGCHC | 2 cut(s) | 505, 1308 |
| SfaNI | GCATC | 4 cut(s) | 367, 697, 766, 1582 |
| SfcI | CTRYAG | 1 cut(s) | 920 |
| SinI | GGWCC | 2 cut(s) | 1250, 1368 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 243, 285, 453, 510, 675 |
| SmlI | CTYRAG | 2 cut(s) | 1010, 1195 |
| SmoI | CTYRAG | 2 cut(s) | 1010, 1195 |
| SseBI | AGGCCT | 1 cut(s) | 1423 |
| SsiI | CCGC | 3 cut(s) | 217, 1366, 1721 |
| SspI | AATATT | 1 cut(s) | 1663 |
| SspMI | CTAG | 6 cut(s) | 243, 704, 864, 942, 1433, 1617 |
| StuI | AGGCCT | 1 cut(s) | 1423 |
| StyD4I | CCNGG | 4 cut(s) | 7, 380, 1534, 1668 |
| StyI | CCWWGG | 2 cut(s) | 673, 1688 |
| TaaI | ACNGT | 5 cut(s) | 254, 341, 427, 452, 1361 |
| TaiI | ACGT | 3 cut(s) | 624, 652, 1568 |
| TaqI | TCGA | 2 cut(s) | 54, 1308 |
| TatI | WGTACW | 1 cut(s) | 1202 |
| TauI | GCSGC | 1 cut(s) | 1723 |
| TfiI | GAWTC | 6 cut(s) | 211, 541, 1154, 1160, 1265, 1289 |
| TscAI | CASTG | 4 cut(s) | 804, 1378, 1536, 1602 |
| TseFI | GTSAC | 1 cut(s) | 1597 |
| Tsp45I | GTSAC | 1 cut(s) | 1597 |
| TspDTI | ATGAA | 8 cut(s) | 41, 249, 291, 312, 515, 606, 1272, 1287 |
| TspRI | CASTG | 4 cut(s) | 804, 1378, 1536, 1602 |
| Vha464I | CTTAAG | 1 cut(s) | 1195 |
| VpaK11BI | GGWCC | 2 cut(s) | 1250, 1368 |
| VspI | ATTAAT | 2 cut(s) | 258, 1256 |
| XbaI | TCTAGA | 1 cut(s) | 703 |
| XceI | RCATGY | 1 cut(s) | 1707 |
| XmiI | GTMKAC | 1 cut(s) | 1356 |
| XmnI | GAANNNNTTC | 1 cut(s) | 949 |
| XspI | CTAG | 6 cut(s) | 243, 704, 864, 942, 1433, 1617 |