No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 858 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2063 |
| AccI | GTMKAC | 1 cut(s) | 2068 |
| AciI | CCGC | 7 cut(s) | 217, 379, 541, 703, 1957, 2108, 2455 |
| AcsI | RAATTY | 7 cut(s) | 442, 622, 1066, 1198, 1490, 1539, 2216 |
| AfaI | GTAC | 3 cut(s) | 2139, 2170, 2396 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 751, 1325, 2063, 2312 |
| AflIII | ACRYGT | 2 cut(s) | 2048, 2162 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 169 |
| AjnI | CCWGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1286, 1318 |
| AleI | CACNNNNGTG | 2 cut(s) | 816, 933 |
| AluBI | AGCT | 8 cut(s) | 675, 1310, 1399, 1620, 1850, 2001, 2278, 2499 |
| AluI | AGCT | 8 cut(s) | 675, 1310, 1399, 1620, 1850, 2001, 2278, 2499 |
| Alw21I | GWGCWC | 2 cut(s) | 1401, 2056 |
| Alw26I | GTCTC | 4 cut(s) | 1593, 1733, 1967, 2069 |
| Alw44I | GTGCAC | 1 cut(s) | 2052 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1184, 2545 |
| Ama87I | CYCGRG | 1 cut(s) | 2141 |
| AoxI | GGCC | 2 cut(s) | 2303, 2484 |
| ApaLI | GTGCAC | 1 cut(s) | 2052 |
| ApeKI | GCWGC | 3 cut(s) | 1178, 1307, 2539 |
| ApoI | RAATTY | 7 cut(s) | 442, 622, 1066, 1198, 1490, 1539, 2216 |
| AsiSI | GCGATCGC | 1 cut(s) | 2431 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 318, 480, 642, 1276 |
| AspS9I | GGNCC | 4 cut(s) | 6, 163, 1748, 2485 |
| AvaI | CYCGRG | 1 cut(s) | 2141 |
| AvaII | GGWCC | 3 cut(s) | 6, 163, 1748 |
| AxyI | CCTNAGG | 1 cut(s) | 1434 |
| BaeGI | GKGCMC | 1 cut(s) | 2056 |
| BamHI | GGATCC | 4 cut(s) | 253, 415, 577, 2149 |
| BanII | GRGCYC | 1 cut(s) | 1401 |
| BauI | CACGAG | 1 cut(s) | 1097 |
| BbsI | GAAGAC | 1 cut(s) | 140 |
| Bbv12I | GWGCWC | 2 cut(s) | 1401, 2056 |
| BbvI | GCAGC | 2 cut(s) | 1190, 1319 |
| BccI | CCATC | 6 cut(s) | 197, 287, 449, 611, 902, 962 |
| BceAI | ACGGC | 1 cut(s) | 1618 |
| BciT130I | CCWGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| BclI | TGATCA | 1 cut(s) | 2415 |
| BcoDI | GTCTC | 4 cut(s) | 1593, 1733, 1967, 2069 |
| BfaI | CTAG | 6 cut(s) | 240, 933, 1170, 1313, 1557, 1821 |
| BfmI | CTRYAG | 3 cut(s) | 900, 1784, 1867 |
| BglII | AGATCT | 1 cut(s) | 2401 |
| BisI | GCNGC | 3 cut(s) | 1179, 1308, 2540 |
| BlsI | GCNGC | 3 cut(s) | 1180, 1309, 2541 |
| Bme1390I | CCNGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| Bme18I | GGWCC | 3 cut(s) | 6, 163, 1748 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 169 |
| BmeT110I | CYCGRG | 1 cut(s) | 2141 |
| BmgT120I | GGNCC | 4 cut(s) | 6, 163, 1748, 2485 |
| BmiI | GGNNCC | 9 cut(s) | 7, 165, 255, 417, 579, 1041, 1380, 1749, 2151 |
| BmrFI | CCNGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| BmrI | ACTGGG | 1 cut(s) | 1125 |
| BmsI | GCATC | 5 cut(s) | 221, 383, 545, 1147, 2278 |
| BmuI | ACTGGG | 1 cut(s) | 1125 |
| BpiI | GAAGAC | 1 cut(s) | 140 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1964, 1996 |
| BpmI | CTGGAG | 6 cut(s) | 35, 1394, 1398, 1596, 2311, 2408 |
| BpuEI | CTTGAG | 4 cut(s) | 304, 796, 2045, 2258 |
| BsaBI | GATNNNNATC | 3 cut(s) | 252, 414, 576 |
| BsaI | GGTCTC | 2 cut(s) | 1593, 1733 |
| BsaJI | CCNNGG | 7 cut(s) | 167, 212, 1020, 1743, 2434, 2487, 2507 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 751, 1325, 2063, 2312 |
| Bse1I | ACTGG | 5 cut(s) | 639, 1120, 1356, 2153, 2474 |
| Bse21I | CCTNAGG | 1 cut(s) | 1434 |
| Bse3DI | GCAATG | 3 cut(s) | 802, 1942, 1966 |
| Bse8I | GATNNNNATC | 3 cut(s) | 252, 414, 576 |
| BseBI | CCWGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| BseDI | CCNNGG | 7 cut(s) | 167, 212, 1020, 1743, 2434, 2487, 2507 |
| BseGI | GGATG | 4 cut(s) | 49, 811, 853, 1084 |
| BseJI | GATNNNNATC | 3 cut(s) | 252, 414, 576 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 751, 1325, 2063, 2312 |
| BseMI | GCAATG | 3 cut(s) | 802, 1942, 1966 |
| BseMII | CTCAG | 3 cut(s) | 272, 434, 1448 |
| BseNI | ACTGG | 5 cut(s) | 639, 1120, 1356, 2153, 2474 |
| BseSI | GKGCMC | 1 cut(s) | 2056 |
| BseXI | GCAGC | 2 cut(s) | 1190, 1319 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2431 |
| BshFI | GGCC | 2 cut(s) | 2305, 2486 |
| BsiEI | CGRYCG | 1 cut(s) | 2431 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1401, 2056 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2141 |
| BslFI | GGGAC | 1 cut(s) | 1761 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 751, 1325, 2063, 2312 |
| BsmAI | GTCTC | 4 cut(s) | 1593, 1733, 1967, 2069 |
| BsmFI | GGGAC | 1 cut(s) | 1761 |
| BsmI | GAATGC | 4 cut(s) | 1096, 1300, 1573, 2110 |
| BsnI | GGCC | 2 cut(s) | 2305, 2486 |
| Bso31I | GGTCTC | 2 cut(s) | 1593, 1733 |
| BsoBI | CYCGRG | 1 cut(s) | 2141 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1401, 2056 |
| Bsp1407I | TGTACA | 1 cut(s) | 2394 |
| BspACI | CCGC | 7 cut(s) | 217, 379, 541, 703, 1957, 2108, 2455 |
| BspANI | GGCC | 2 cut(s) | 2305, 2486 |
| BspCNI | CTCAG | 3 cut(s) | 271, 433, 1447 |
| BspHI | TCATGA | 4 cut(s) | 112, 151, 384, 546 |
| BspLI | GGNNCC | 9 cut(s) | 7, 165, 255, 417, 579, 1041, 1380, 1749, 2151 |
| BspMAI | CTGCAG | 1 cut(s) | 1788 |
| BspQI | GCTCTTC | 1 cut(s) | 1389 |
| BspTNI | GGTCTC | 2 cut(s) | 1593, 1733 |
| BsrDI | GCAATG | 3 cut(s) | 802, 1942, 1966 |
| BsrGI | TGTACA | 1 cut(s) | 2394 |
| BsrI | ACTGG | 5 cut(s) | 639, 1120, 1356, 2153, 2474 |
| BssECI | CCNNGG | 7 cut(s) | 167, 212, 1020, 1743, 2434, 2487, 2507 |
| BssSI | CACGAG | 1 cut(s) | 1097 |
| BssT1I | CCWWGG | 3 cut(s) | 1020, 1743, 2507 |
| Bst2BI | CACGAG | 1 cut(s) | 1097 |
| Bst2UI | CCWGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| Bst4CI | ACNGT | 2 cut(s) | 990, 2547 |
| Bst6I | CTCTTC | 3 cut(s) | 704, 1389, 1731 |
| BstAUI | TGTACA | 1 cut(s) | 2394 |
| BstC8I | GCNNGC | 2 cut(s) | 2307, 2425 |
| BstDEI | CTNAG | 7 cut(s) | 174, 258, 420, 1434, 1707, 2015, 2281 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2310 |
| BstF5I | GGATG | 4 cut(s) | 49, 811, 853, 1084 |
| BstMAI | GTCTC | 4 cut(s) | 1593, 1733, 1967, 2069 |
| BstMCI | CGRYCG | 1 cut(s) | 2431 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1304, 2275 |
| BstNI | CCWGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| BstNSI | RCATGY | 2 cut(s) | 2052, 2166 |
| BstSCI | CCNGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| BstSFI | CTRYAG | 3 cut(s) | 900, 1784, 1867 |
| BstSLI | GKGCMC | 1 cut(s) | 2056 |
| BstV1I | GCAGC | 2 cut(s) | 1190, 1319 |
| BstV2I | GAAGAC | 1 cut(s) | 140 |
| BstX2I | RGATCY | 9 cut(s) | 253, 415, 577, 1258, 1315, 1798, 2149, 2239, 2401 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 365, 527, 689, 1151 |
| BstYI | RGATCY | 9 cut(s) | 253, 415, 577, 1258, 1315, 1798, 2149, 2239, 2401 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1434 |
| BsuRI | GGCC | 2 cut(s) | 2305, 2486 |
| BtsCI | GGATG | 4 cut(s) | 49, 811, 853, 1084 |
| BtsIMutI | CAGTG | 4 cut(s) | 632, 1182, 1287, 1518 |
| Cac8I | GCNNGC | 2 cut(s) | 2307, 2425 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1184, 2545 |
| CciI | TCATGA | 4 cut(s) | 112, 151, 384, 546 |
| Cfr13I | GGNCC | 4 cut(s) | 6, 163, 1748, 2485 |
| CsiI | ACCWGGT | 1 cut(s) | 1897 |
| Csp6I | GTAC | 3 cut(s) | 2138, 2169, 2395 |
| CviQI | GTAC | 3 cut(s) | 2138, 2169, 2395 |
| DdeI | CTNAG | 7 cut(s) | 174, 258, 420, 1434, 1707, 2015, 2281 |
| DraI | TTTAAA | 2 cut(s) | 1254, 1728 |
| DriI | GACNNNNNGTC | 1 cut(s) | 169 |
| Eam1104I | CTCTTC | 3 cut(s) | 704, 1389, 1731 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 169 |
| EarI | CTCTTC | 3 cut(s) | 704, 1389, 1731 |
| EciI | GGCGGA | 2 cut(s) | 394, 556 |
| Ecl136II | GAGCTC | 1 cut(s) | 1399 |
| Eco130I | CCWWGG | 3 cut(s) | 1020, 1743, 2507 |
| Eco147I | AGGCCT | 1 cut(s) | 2305 |
| Eco24I | GRGCYC | 1 cut(s) | 1401 |
| Eco31I | GGTCTC | 2 cut(s) | 1593, 1733 |
| Eco32I | GATATC | 1 cut(s) | 835 |
| Eco47I | GGWCC | 3 cut(s) | 6, 163, 1748 |
| Eco53kI | GAGCTC | 1 cut(s) | 1399 |
| Eco81I | CCTNAGG | 1 cut(s) | 1434 |
| Eco88I | CYCGRG | 1 cut(s) | 2141 |
| EcoICRI | GAGCTC | 1 cut(s) | 1399 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2310 |
| EcoRI | GAATTC | 1 cut(s) | 1198 |
| EcoRII | CCWGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| EcoRV | GATATC | 1 cut(s) | 835 |
| EcoT14I | CCWWGG | 3 cut(s) | 1020, 1743, 2507 |
| EcoT22I | ATGCAT | 5 cut(s) | 236, 310, 398, 472, 560 |
| EcoT38I | GRGCYC | 1 cut(s) | 1401 |
| ErhI | CCWWGG | 3 cut(s) | 1020, 1743, 2507 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 167, 199, 257, 289, 419, 451 |
| FaqI | GGGAC | 1 cut(s) | 1761 |
| FauI | CCCGC | 3 cut(s) | 210, 696, 2448 |
| FauNDI | CATATG | 5 cut(s) | 304, 466, 628, 2080, 2353 |
| FbaI | TGATCA | 1 cut(s) | 2415 |
| FblI | GTMKAC | 1 cut(s) | 2068 |
| Fnu4HI | GCNGC | 3 cut(s) | 1179, 1308, 2540 |
| FokI | GGATG | 4 cut(s) | 56, 818, 860, 1091 |
| FriOI | GRGCYC | 1 cut(s) | 1401 |
| Fsp4HI | GCNGC | 3 cut(s) | 1179, 1308, 2540 |
| FspBI | CTAG | 6 cut(s) | 240, 933, 1170, 1313, 1557, 1821 |
| GluI | GCNGC | 3 cut(s) | 1179, 1308, 2540 |
| GsuI | CTGGAG | 6 cut(s) | 35, 1394, 1398, 1596, 2311, 2408 |
| HaeIII | GGCC | 2 cut(s) | 2305, 2486 |
| HindIII | AAGCTT | 2 cut(s) | 1618, 2497 |
| Hpy166II | GTNNAC | 7 cut(s) | 9, 163, 1239, 2054, 2069, 2089, 2395 |
| Hpy8I | GTNNAC | 7 cut(s) | 9, 163, 1239, 2054, 2069, 2089, 2395 |
| HpyAV | CCTTC | 4 cut(s) | 1834, 2176, 2230, 2486 |
| HpyCH4III | ACNGT | 2 cut(s) | 990, 2547 |
| HpyCH4IV | ACGT | 1 cut(s) | 2248 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1304, 2275 |
| HpyF3I | CTNAG | 7 cut(s) | 174, 258, 420, 1434, 1707, 2015, 2281 |
| HpySE526I | ACGT | 1 cut(s) | 2248 |
| Ksp22I | TGATCA | 1 cut(s) | 2415 |
| LguI | GCTCTTC | 1 cut(s) | 1389 |
| Lsp1109I | GCAGC | 2 cut(s) | 1190, 1319 |
| LweI | GCATC | 5 cut(s) | 221, 383, 545, 1147, 2278 |
| MabI | ACCWGGT | 1 cut(s) | 1897 |
| MaeI | CTAG | 6 cut(s) | 240, 933, 1170, 1313, 1557, 1821 |
| MaeII | ACGT | 1 cut(s) | 2248 |
| MaeIII | GTNAC | 2 cut(s) | 1952, 2207 |
| MflI | RGATCY | 9 cut(s) | 253, 415, 577, 1258, 1315, 1798, 2149, 2239, 2401 |
| MhlI | GDGCHC | 2 cut(s) | 1401, 2056 |
| MlyI | GAGTC | 2 cut(s) | 1213, 1973 |
| MmeI | TCCRAC | 1 cut(s) | 717 |
| Mph1103I | ATGCAT | 5 cut(s) | 236, 310, 398, 472, 560 |
| MroXI | GAANNNNTTC | 4 cut(s) | 318, 480, 642, 1276 |
| MseI | TTAA | 9 cut(s) | 786, 954, 1253, 1727, 1760, 2132, 2178, 2220, 2244 |
| MslI | CAYNNNNRTG | 6 cut(s) | 117, 156, 795, 816, 933, 969 |
| MspR9I | CCNGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| Mva1269I | GAATGC | 4 cut(s) | 1096, 1300, 1573, 2110 |
| MvaI | CCWGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1304, 2275 |
| NdeI | CATATG | 5 cut(s) | 304, 466, 628, 2080, 2353 |
| NlaIV | GGNNCC | 9 cut(s) | 7, 165, 255, 417, 579, 1041, 1380, 1749, 2151 |
| NmeAIII | GCCGAG | 1 cut(s) | 237 |
| NmuCI | GTSAC | 2 cut(s) | 1952, 2207 |
| NsiI | ATGCAT | 5 cut(s) | 236, 310, 398, 472, 560 |
| NspI | RCATGY | 2 cut(s) | 2052, 2166 |
| OliI | CACNNNNGTG | 2 cut(s) | 816, 933 |
| PaeR7I | CTCGAG | 1 cut(s) | 2141 |
| PagI | TCATGA | 4 cut(s) | 112, 151, 384, 546 |
| PceI | AGGCCT | 1 cut(s) | 2305 |
| PciI | ACATGT | 2 cut(s) | 2048, 2162 |
| PciSI | GCTCTTC | 1 cut(s) | 1389 |
| PctI | GAATGC | 4 cut(s) | 1096, 1300, 1573, 2110 |
| PdmI | GAANNNNTTC | 4 cut(s) | 318, 480, 642, 1276 |
| PfeI | GAWTC | 8 cut(s) | 47, 104, 1546, 1778, 2123, 2186, 2446, 2460 |
| PflFI | GACNNNGTC | 1 cut(s) | 1368 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2063 |
| PkrI | GCNGC | 3 cut(s) | 1180, 1309, 2541 |
| Ple19I | CGATCG | 1 cut(s) | 2431 |
| PleI | GAGTC | 2 cut(s) | 1213, 1973 |
| PpsI | GAGTC | 2 cut(s) | 1213, 1973 |
| PscI | ACATGT | 2 cut(s) | 2048, 2162 |
| PsiI | TTATAA | 1 cut(s) | 858 |
| Psp124BI | GAGCTC | 1 cut(s) | 1401 |
| Psp6I | CCWGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| PspGI | CCWGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| PspN4I | GGNNCC | 9 cut(s) | 7, 165, 255, 417, 579, 1041, 1380, 1749, 2151 |
| PspPI | GGNCC | 4 cut(s) | 6, 163, 1748, 2485 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 254, 286 |
| PstI | CTGCAG | 1 cut(s) | 1788 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1184, 2545 |
| PsuI | RGATCY | 9 cut(s) | 253, 415, 577, 1258, 1315, 1798, 2149, 2239, 2401 |
| PsyI | GACNNNGTC | 1 cut(s) | 1368 |
| PvuI | CGATCG | 1 cut(s) | 2431 |
| RgaI | GCGATCGC | 1 cut(s) | 2431 |
| RsaI | GTAC | 3 cut(s) | 2139, 2170, 2396 |
| RsaNI | GTAC | 3 cut(s) | 2138, 2169, 2395 |
| RseI | CAYNNNNRTG | 6 cut(s) | 117, 156, 795, 816, 933, 969 |
| SacI | GAGCTC | 1 cut(s) | 1401 |
| SapI | GCTCTTC | 1 cut(s) | 1389 |
| SaqAI | TTAA | 9 cut(s) | 786, 954, 1253, 1727, 1760, 2132, 2178, 2220, 2244 |
| SatI | GCNGC | 3 cut(s) | 1179, 1308, 2540 |
| Sau96I | GGNCC | 4 cut(s) | 6, 163, 1748, 2485 |
| SchI | GAGTC | 2 cut(s) | 1213, 1973 |
| ScrFI | CCNGG | 4 cut(s) | 1899, 2290, 2436, 2489 |
| SduI | GDGCHC | 2 cut(s) | 1401, 2056 |
| SexAI | ACCWGGT | 1 cut(s) | 1897 |
| SfaAI | GCGATCGC | 1 cut(s) | 2431 |
| SfaNI | GCATC | 5 cut(s) | 221, 383, 545, 1147, 2278 |
| SfcI | CTRYAG | 3 cut(s) | 900, 1784, 1867 |
| Sfr274I | CTCGAG | 1 cut(s) | 2141 |
| SgfI | GCGATCGC | 1 cut(s) | 2431 |
| SinI | GGWCC | 3 cut(s) | 6, 163, 1748 |
| SlaI | CTCGAG | 1 cut(s) | 2141 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 117, 156, 795, 816, 933, 969 |
| SmlI | CTYRAG | 5 cut(s) | 283, 775, 2024, 2141, 2273 |
| SmoI | CTYRAG | 5 cut(s) | 283, 775, 2024, 2141, 2273 |
| SseBI | AGGCCT | 1 cut(s) | 2305 |
| SsiI | CCGC | 7 cut(s) | 217, 379, 541, 703, 1957, 2108, 2455 |
| SspMI | CTAG | 6 cut(s) | 240, 933, 1170, 1313, 1557, 1821 |
| SstI | GAGCTC | 1 cut(s) | 1401 |
| StuI | AGGCCT | 1 cut(s) | 2305 |
| StyD4I | CCNGG | 4 cut(s) | 1897, 2288, 2434, 2487 |
| StyI | CCWWGG | 3 cut(s) | 1020, 1743, 2507 |
| TaaI | ACNGT | 2 cut(s) | 990, 2547 |
| TaiI | ACGT | 1 cut(s) | 2251 |
| TaqI | TCGA | 4 cut(s) | 50, 1205, 2142, 2502 |
| TaqII | GACCGA | 1 cut(s) | 159 |
| TatI | WGTACW | 2 cut(s) | 2137, 2394 |
| TfiI | GAWTC | 8 cut(s) | 47, 104, 1546, 1778, 2123, 2186, 2446, 2460 |
| Tru1I | TTAA | 9 cut(s) | 786, 954, 1253, 1727, 1760, 2132, 2178, 2220, 2244 |
| Tru9I | TTAA | 9 cut(s) | 786, 954, 1253, 1727, 1760, 2132, 2178, 2220, 2244 |
| TscAI | CASTG | 4 cut(s) | 639, 1189, 1287, 1518 |
| TseFI | GTSAC | 2 cut(s) | 1952, 2207 |
| TseI | GCWGC | 3 cut(s) | 1178, 1307, 2539 |
| Tsp45I | GTSAC | 2 cut(s) | 1952, 2207 |
| TspRI | CASTG | 4 cut(s) | 639, 1189, 1287, 1518 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1368 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2063 |
| VneI | GTGCAC | 1 cut(s) | 2052 |
| VpaK11BI | GGWCC | 3 cut(s) | 6, 163, 1748 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2310 |
| XapI | RAATTY | 7 cut(s) | 442, 622, 1066, 1198, 1490, 1539, 2216 |
| XbaI | TCTAGA | 1 cut(s) | 1312 |
| XceI | RCATGY | 2 cut(s) | 2052, 2166 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1057 |
| XhoI | CTCGAG | 1 cut(s) | 2141 |
| XmiI | GTMKAC | 1 cut(s) | 2068 |
| XmnI | GAANNNNTTC | 4 cut(s) | 318, 480, 642, 1276 |
| XspI | CTAG | 6 cut(s) | 240, 933, 1170, 1313, 1557, 1821 |
| Zsp2I | ATGCAT | 5 cut(s) | 236, 310, 398, 472, 560 |