Prupe.1G488800_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000931
Physical Location & Seq
Forward (+)
40697929 .. 40702578
4650 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G488800.1

Sequence Viewer

Length: 1431 bp
ATGGCGCCTTATAAGGGGATACGAGAGCTCAACAGTTTAAAGAAGGATTATGAGGGAGATTGTGAGGATGACAAGAGGTTGGCCAGGAGGAGGAAGAGAAAGTGCATTGCTGATAGGAATTTTGAGCCCAAATCCTGGTTCAGTGCCACGAAGAAAAGAAGAGACAGTAGTATCCAAGGGGAAGAAGAAATGGCGGATAAGGATTTTGAGAAATCATTGAATGGTTTAGATCTTTATGGTGATTGGGTTGATGATGATGTGGATCCTGACTATAAGAAGTTCTTGGAAAATCTGACGGAAGATGGAAGCTCCTATGTACTGGTGGTTGTTCGAGAGAATGAGAATTTAGAGCTGATAAAGTTTGAACAAGAAGATGGGGAACTAGATGAGACCATTTTAGATACCCCAGAAACTTTGAAAAAATCTCAGATGAAAAAGAAGACTGAAATAAAAAAAGCTTTGAGGGGTGGTATAAAGAGGGAACAAATTTATTGCTTTAACGATTCTGTTATTCCTTTGAATAAAGAAGAGTTTAAGAGCCCAAGATCTGTTAGGTATGCTATGAGTAAAAAAACAAGGGGAATTCAGGAAGATTCAGAGGGTCTGTCAAAGAAAAAGAGTTCAGGGGTGAAGAAGAATGTAAATGTGGAAGCTCCGGATCCTGTTTCTGATAGGACAAAAGGGCGTTCTAATAAAATCCATGGTTTAGAGGTTCCCACTACTCGAGAAACTTTAAAAAGTTATCATGTGAAGAAGAAGAATGTAAATAAGAAAGGTGCAGATGGTGGGACAAAGGGGCTTCCTGTTAAAACACCTCATTTGGCTGAGAGTGGACACAACCATGGAGCTGCGTCTGATCAAATAGACACGGACATGGACTATCAAGAGTTTCTAGATGGTCTAAGAAAATATGGTGGAAAAGTGGTGTATACACCTACAACTGGTGGACCAGTGGCAGTTGTACTTGAGGAGGATGTAGAGAGTAAAAATGAGTATTTTACCTCATTTACGGCAGTGGACGTAGACAGTGGGTGGTGTGTTGAAACTTGTGATACAAGTCATGCCCAGTTTAGGAAGGGGCTTATGAAGGATCTTAAACGGCCTTATGACCAAGAAGAGTATAAAAGGCTTTTGAAAGAACTGACCCTACGAAGGCCAATAAATCACGACAGAAATCTGCGCAATGGAAGAACAAAATCCTACCCAGTTGCAGGGAAATTAGGAGCTTCATATCGAGAGCAGCACATAGTCCTGGCAAGAAAGATTGATGCTGCTGGTAGCGACCGCCCTAGAATTTTGAATCTTTTGCGTGGATTTTTCTACTGGCTGAAGAATGTGGCGCAAGAAGGATGCTTCTGTCCTTGGAGTGACTCATCTTGTTTGAAGCTGTTGCCTCAAGCTGATAGAAAAAGGAAGTTTAGCCGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

477

Amino Acids

54.48

Weight (kDa)

9.02

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
Acc16I TGCGCA 1 cut(s) 1181
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 135
AccI GTMKAC 2 cut(s) 929, 1023
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 3 cut(s) 194, 1285, 1423
AclWI GGATC 5 cut(s) 257, 270, 653, 666, 1098
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 5 cut(s) 118, 343, 486, 582, 1293
AcuI CTGAAG 1 cut(s) 1349
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 318, 963
AfiI CCNNNNNNNGG 7 cut(s) 14, 90, 135, 941, 1071, 1152, 1211
AgsI TTSAA 8 cut(s) 220, 365, 418, 520, 1043, 1135, 1300, 1384
AjnI CCWGG 3 cut(s) 83, 134, 1251
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 9 cut(s) 28, 309, 352, 458, 653, 848, 1226, 1387, 1400
AluI AGCT 9 cut(s) 28, 309, 352, 458, 653, 848, 1226, 1387, 1400
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 156, 383
AlwI GGATC 5 cut(s) 257, 270, 653, 666, 1098
Ama87I CYCGRG 1 cut(s) 723
Aor13HI TCCGGA 1 cut(s) 655
AoxI GGCC 3 cut(s) 81, 1100, 1154
ApeKI GCWGC 3 cut(s) 848, 1240, 1271
ApoI RAATTY 5 cut(s) 118, 343, 486, 582, 1293
AspLEI GCGC 3 cut(s) 7, 1182, 1342
AspS9I GGNCC 1 cut(s) 947
AsuHPI GGTGA 2 cut(s) 251, 640
AvaI CYCGRG 1 cut(s) 723
AvaII GGWCC 1 cut(s) 947
BaeI ACNNNNGTAYC 2 cut(s) 154, 187
BalI TGGCCA 1 cut(s) 83
BamHI GGATCC 2 cut(s) 262, 658
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 30, 129, 542
BbsI GAAGAC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 3 cut(s) 835, 1252, 1258
BccI CCATC 4 cut(s) 296, 368, 776, 890
BceAI ACGGC 2 cut(s) 1026, 1115
BciT130I CCWGG 3 cut(s) 85, 136, 1253
BciVI GTATCC 2 cut(s) 12, 182
BclI TGATCA 1 cut(s) 856
BcoDI GTCTC 2 cut(s) 156, 383
BfaI CTAG 3 cut(s) 383, 893, 1290
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 2 cut(s) 12, 182
BglII AGATCT 2 cut(s) 229, 545
BisI GCNGC 4 cut(s) 849, 1241, 1272, 1423
BlsI GCNGC 4 cut(s) 850, 1242, 1273, 1424
Bme1390I CCNGG 3 cut(s) 85, 136, 1253
Bme18I GGWCC 1 cut(s) 947
BmeT110I CYCGRG 1 cut(s) 723
BmgT120I GGNCC 1 cut(s) 947
BmiI GGNNCC 4 cut(s) 6, 264, 660, 714
BmrFI CCNGG 3 cut(s) 85, 136, 1253
BmrI ACTGGG 2 cut(s) 1060, 1199
BmsI GCATC 2 cut(s) 1258, 1340
BmuI ACTGGG 2 cut(s) 1060, 1199
BpiI GAAGAC 1 cut(s) 446
BpuEI CTTGAG 2 cut(s) 986, 1380
BsaBI GATNNNNATC 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 175, 700, 841, 1361
BsaWI WCCGGW 1 cut(s) 655
Bsc4I CCNNNNNNNGG 7 cut(s) 14, 90, 135, 941, 1071, 1152, 1211
Bse1I ACTGG 6 cut(s) 324, 946, 950, 1066, 1205, 1328
Bse3DI GCAATG 2 cut(s) 105, 1189
Bse8I GATNNNNATC 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 3 cut(s) 85, 136, 1253
BseDI CCNNGG 4 cut(s) 175, 700, 841, 1361
BseGI GGATG 3 cut(s) 73, 979, 1355
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 7 cut(s) 14, 90, 135, 941, 1071, 1152, 1211
BseMI GCAATG 2 cut(s) 105, 1189
BseMII CTCAG 2 cut(s) 440, 816
BseNI ACTGG 6 cut(s) 324, 946, 950, 1066, 1205, 1328
BseRI GAGGAG 2 cut(s) 103, 983
BseXI GCAGC 3 cut(s) 835, 1252, 1258
BsgI GTGCAG 1 cut(s) 798
Bsh1285I CGRYCG 1 cut(s) 1285
BshFI GGCC 3 cut(s) 83, 1102, 1156
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 1285
BsiHKAI GWGCWC 1 cut(s) 30
BsiHKCI CYCGRG 1 cut(s) 723
BsiSI CCGG 1 cut(s) 656
BslFI GGGAC 1 cut(s) 802
BslI CCNNNNNNNGG 7 cut(s) 14, 90, 135, 941, 1071, 1152, 1211
BsmAI GTCTC 2 cut(s) 156, 383
BsmFI GGGAC 1 cut(s) 802
BsnI GGCC 3 cut(s) 83, 1102, 1156
Bso31I GGTCTC 1 cut(s) 383
BsoBI CYCGRG 1 cut(s) 723
Bsp1286I GDGCHC 3 cut(s) 30, 129, 542
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
Bsp19I CCATGG 2 cut(s) 700, 841
BspACI CCGC 3 cut(s) 194, 1285, 1423
BspANI GGCC 3 cut(s) 83, 1102, 1156
BspCNI CTCAG 2 cut(s) 439, 817
BspEI TCCGGA 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 6, 264, 660, 714
BspPI GGATC 5 cut(s) 257, 270, 653, 666, 1098
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 2 cut(s) 105, 1189
BsrI ACTGG 6 cut(s) 324, 946, 950, 1066, 1205, 1328
BssECI CCNNGG 4 cut(s) 175, 700, 841, 1361
BssMI GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
BssNAI GTATAC 1 cut(s) 930
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 175, 700, 841, 1361
Bst1107I GTATAC 1 cut(s) 930
Bst2UI CCWGG 3 cut(s) 85, 136, 1253
Bst4CI ACNGT 3 cut(s) 35, 167, 1028
Bst6I CTCTTC 4 cut(s) 89, 154, 522, 1110
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 3 cut(s) 426, 825, 902
BstDSI CCRYGG 2 cut(s) 700, 841
BstF5I GGATG 3 cut(s) 73, 979, 1355
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 1182, 1342
BstKTI GATC 6 cut(s) 232, 265, 548, 661, 859, 1093
BstMAI GTCTC 2 cut(s) 156, 383
BstMBI GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
BstMCI CGRYCG 1 cut(s) 1285
BstNI CCWGG 3 cut(s) 85, 136, 1253
BstNSI RCATGY 1 cut(s) 1429
BstSCI CCNGG 3 cut(s) 83, 134, 1251
BstV1I GCAGC 3 cut(s) 835, 1252, 1258
BstV2I GAAGAC 1 cut(s) 446
BstX2I RGATCY 5 cut(s) 229, 262, 545, 658, 1090
BstYI RGATCY 5 cut(s) 229, 262, 545, 658, 1090
BstZ17I GTATAC 1 cut(s) 930
BsuI GTATCC 2 cut(s) 12, 182
BsuRI GGCC 3 cut(s) 83, 1102, 1156
BtgI CCRYGG 2 cut(s) 700, 841
BtsCI GGATG 3 cut(s) 73, 979, 1355
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 4 cut(s) 148, 957, 1020, 1033
CfoI GCGC 3 cut(s) 7, 1182, 1342
Cfr13I GGNCC 1 cut(s) 947
CseI GACGC 1 cut(s) 840
Csp6I GTAC 2 cut(s) 317, 962
CviAII CATG 6 cut(s) 701, 746, 842, 874, 1061, 1426
CviQI GTAC 2 cut(s) 317, 962
DdeI CTNAG 3 cut(s) 426, 825, 902
DinI GGCGCC 1 cut(s) 6
DpnI GATC 6 cut(s) 231, 264, 547, 660, 858, 1092
DpnII GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
DraI TTTAAA 2 cut(s) 39, 735
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 4 cut(s) 89, 154, 522, 1110
EarI CTCTTC 4 cut(s) 89, 154, 522, 1110
EciI GGCGGA 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 4 cut(s) 175, 700, 841, 1361
Eco24I GRGCYC 3 cut(s) 30, 129, 542
Eco31I GGTCTC 1 cut(s) 383
Eco47I GGWCC 1 cut(s) 947
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 1349
Eco88I CYCGRG 1 cut(s) 723
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 582
EcoRII CCWGG 3 cut(s) 83, 134, 1251
EcoT14I CCWWGG 4 cut(s) 175, 700, 841, 1361
EcoT38I GRGCYC 3 cut(s) 30, 129, 542
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 175, 700, 841, 1361
FaeI CATG 6 cut(s) 704, 749, 845, 877, 1064, 1429
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 1 cut(s) 802
FatI CATG 6 cut(s) 700, 745, 841, 873, 1060, 1425
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 2 cut(s) 929, 1023
Fnu4HI GCNGC 4 cut(s) 849, 1241, 1272, 1423
FokI GGATG 3 cut(s) 80, 986, 1362
FriOI GRGCYC 3 cut(s) 30, 129, 542
Fsp4HI GCNGC 4 cut(s) 849, 1241, 1272, 1423
FspBI CTAG 3 cut(s) 383, 893, 1290
FspI TGCGCA 1 cut(s) 1181
GlaI GCGC 3 cut(s) 6, 1181, 1341
GluI GCNGC 4 cut(s) 849, 1241, 1272, 1423
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 83, 1102, 1156
HapII CCGG 1 cut(s) 656
HgaI GACGC 1 cut(s) 840
HhaI GCGC 3 cut(s) 7, 1182, 1342
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 704, 749, 845, 877, 1064, 1429
Hin6I GCGC 3 cut(s) 5, 1180, 1340
HinP1I GCGC 3 cut(s) 5, 1180, 1340
HindIII AAGCTT 1 cut(s) 456
HinfI GANTC 4 cut(s) 503, 593, 1300, 1370
HpaII CCGG 1 cut(s) 656
HphI GGTGA 2 cut(s) 251, 640
Hpy166II GTNNAC 5 cut(s) 833, 930, 947, 1018, 1024
Hpy188I TCNGA 5 cut(s) 294, 429, 598, 670, 856
Hpy188III TCNNGA 9 cut(s) 266, 332, 587, 656, 725, 884, 893, 1166, 1235
Hpy8I GTNNAC 5 cut(s) 833, 930, 947, 1018, 1024
HpyAV CCTTC 5 cut(s) 37, 1069, 1081, 1146, 1340
HpyCH4III ACNGT 3 cut(s) 35, 167, 1028
HpyCH4IV ACGT 1 cut(s) 1020
HpyCH4V TGCA 3 cut(s) 105, 779, 1211
HpyF3I CTNAG 3 cut(s) 426, 825, 902
HpySE526I ACGT 1 cut(s) 1020
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 704, 749, 845, 877, 1064, 1429
HspAI GCGC 3 cut(s) 5, 1180, 1340
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 655
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
LmnI GCTCC 4 cut(s) 314, 658, 845, 1223
Lsp1109I GCAGC 3 cut(s) 835, 1252, 1258
LweI GCATC 2 cut(s) 1258, 1340
MaeI CTAG 3 cut(s) 383, 893, 1290
MaeII ACGT 1 cut(s) 1020
MaeIII GTNAC 1 cut(s) 1367
MalI GATC 6 cut(s) 231, 264, 547, 660, 858, 1092
MboI GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
MflI RGATCY 5 cut(s) 229, 262, 545, 658, 1090
MhlI GDGCHC 3 cut(s) 30, 129, 542
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 6 cut(s) 118, 343, 486, 582, 1217, 1293
MluNI TGGCCA 1 cut(s) 83
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1364
Mox20I TGGCCA 1 cut(s) 83
MroI TCCGGA 1 cut(s) 655
MscI TGGCCA 1 cut(s) 83
MseI TTAA 6 cut(s) 38, 498, 534, 734, 807, 1095
MslI CAYNNNNRTG 2 cut(s) 840, 872
Msp20I TGGCCA 1 cut(s) 83
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 3 cut(s) 85, 136, 1253
MvaI CCWGG 3 cut(s) 85, 136, 1253
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 2 cut(s) 700, 841
NdeII GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
NlaIII CATG 6 cut(s) 704, 749, 845, 877, 1064, 1429
NlaIV GGNNCC 4 cut(s) 6, 264, 660, 714
NmuCI GTSAC 1 cut(s) 1367
NsbI TGCGCA 1 cut(s) 1181
NspI RCATGY 1 cut(s) 1429
PaeR7I CTCGAG 1 cut(s) 723
PfeI GAWTC 3 cut(s) 503, 593, 1300
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 4 cut(s) 850, 1242, 1273, 1424
PleI GAGTC 1 cut(s) 1364
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 1364
PsiI TTATAA 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 3 cut(s) 83, 134, 1251
PspGI CCWGG 3 cut(s) 83, 134, 1251
PspN4I GGNNCC 4 cut(s) 6, 264, 660, 714
PspPI GGNCC 1 cut(s) 947
PsrI GAACNNNNNNTAC 2 cut(s) 1131, 1163
PsuI RGATCY 5 cut(s) 229, 262, 545, 658, 1090
RsaI GTAC 2 cut(s) 318, 963
RsaNI GTAC 2 cut(s) 317, 962
RseI CAYNNNNRTG 2 cut(s) 840, 872
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 6 cut(s) 38, 498, 534, 734, 807, 1095
SatI GCNGC 4 cut(s) 849, 1241, 1272, 1423
Sau3AI GATC 6 cut(s) 229, 262, 545, 658, 856, 1090
Sau96I GGNCC 1 cut(s) 947
SchI GAGTC 1 cut(s) 1364
ScrFI CCNGG 3 cut(s) 85, 136, 1253
SduI GDGCHC 3 cut(s) 30, 129, 542
SfaNI GCATC 2 cut(s) 1258, 1340
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 723
SinI GGWCC 1 cut(s) 947
SlaI CTCGAG 1 cut(s) 723
SmiMI CAYNNNNRTG 2 cut(s) 840, 872
SmlI CTYRAG 3 cut(s) 723, 965, 1395
SmoI CTYRAG 3 cut(s) 723, 965, 1395
Sse9I AATT 6 cut(s) 118, 343, 486, 582, 1217, 1293
SsiI CCGC 3 cut(s) 194, 1285, 1423
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 383, 893, 1290
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 83, 134, 1251
StyI CCWWGG 4 cut(s) 175, 700, 841, 1361
TaaI ACNGT 3 cut(s) 35, 167, 1028
TaiI ACGT 1 cut(s) 1023
TaqI TCGA 3 cut(s) 331, 724, 1234
TasI AATT 6 cut(s) 118, 343, 486, 582, 1217, 1293
TatI WGTACW 2 cut(s) 316, 961
TauI GCSGC 1 cut(s) 1425
TfiI GAWTC 3 cut(s) 503, 593, 1300
Tru1I TTAA 6 cut(s) 38, 498, 534, 734, 807, 1095
Tru9I TTAA 6 cut(s) 38, 498, 534, 734, 807, 1095
TscAI CASTG 4 cut(s) 148, 957, 1020, 1033
TseFI GTSAC 1 cut(s) 1367
TseI GCWGC 3 cut(s) 848, 1240, 1271
Tsp45I GTSAC 1 cut(s) 1367
TspDTI ATGAA 3 cut(s) 446, 1100, 1218
TspGWI ACGGA 2 cut(s) 311, 884
TspRI CASTG 4 cut(s) 148, 957, 1020, 1033
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 947
XapI RAATTY 5 cut(s) 118, 343, 486, 582, 1293
XbaI TCTAGA 1 cut(s) 892
XceI RCATGY 1 cut(s) 1429
XhoI CTCGAG 1 cut(s) 723
XmiI GTMKAC 2 cut(s) 929, 1023
XspI CTAG 3 cut(s) 383, 893, 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.