Rmu_co8494021.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8494021.1
OG0000931
Physical Location & Seq
Reverse (-)
572 .. 2641
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8494021.1_g000001.1.cds

Sequence Viewer

Length: 1295 bp
gtggtggtaggaatggacgatgtggcgtggatgatggtgatggtgggaatgctcattttggtgatggcaacattcatgttaattctttgaatcatggcgtcgatgatgatgatatggatcctcagtataagatattctgggacaatttgaaggaagatggaaattcatacgtactggagatggttcaagaggatggacgtgtgaaacagataaggtatcaccaaggcgatggtttacctgatgagctcgatctagatgccacgcattcccagggaaaacagaagactgaatttaaaaacagggagactatggaaaatttagaggatgccccagagaaaaggttttcagtgaggaagaggaatgtaaaattggaagctctggaacctattttagacggaacaaatgtgtgttctagtaaacgacctcgtttagaggttccaaacactccaaaaagtttaggaagttctccacagaaagagaaaactgaaactagaagaaattcaaggagtgtcattaagaagagaaatttagaggatcgtccggcgaaaaagaatggtcttgagaaaaagagttcaatgttgaagaagaatgtgaaggaagcagatgcagatcctctttctggtagagcaaatgggcgttctcataaacagtctggttcaaaatatctttggttctcagagggtgggtgcaatagtgaagctgaatctgattctaatctaacagatgacagctatcaagagttcttaagttgtactaaggatgatgggaaagttatggtatttacatccagaatttgtggaccattaatatatgattcagatgaagaaagttcatcagattcggatgtgatcgtgctcgacagggatccacatgagggataccgtacaccaattattgatgtagacagtcggcggtccactgaaagtcattcccagtttaggcaagggattatgcaggatcttaaaaggccttaccaccaagaagagtatatcaatctcttggaagactggtcttgccaaaggccagccacaggtcatgatagaaatctacgaaatggaaatgtaaaatcatatgcactgccgggggaatttgggaaatcatatcaagagatgtacaaagatctggcgggaaagattaatgcagtgacaggcgatgatcgccctagaaagttaaatcttttgcgtggatttttctactggttgaagaatatttcccaggaaggaagctttaaaccttggaatgacaaagcatgtttgaaggtggtgccgcagacactaaataggaggacttcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

48.81

Weight (kDa)

6.27

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1263
AccI GTMKAC 1 cut(s) 902
AciI CCGC 3 cut(s) 912, 1126, 1267
AclWI GGATC 7 cut(s) 112, 125, 542, 604, 859, 872, 965
AcsI RAATTY 7 cut(s) 162, 289, 315, 498, 525, 791, 1087
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 4 cut(s) 173, 753, 885, 1114
AfiI CCNNNNNNNGG 4 cut(s) 619, 874, 938, 1030
AflII CTTAAG 1 cut(s) 744
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 9 cut(s) 90, 150, 187, 503, 575, 582, 659, 1203, 1257
AjiI CACGTC 1 cut(s) 199
AjnI CCWGG 2 cut(s) 269, 1214
AjuI GAANNNNNNNTTGG 2 cut(s) 352, 384
AluBI AGCT 5 cut(s) 246, 376, 700, 731, 1226
AluI AGCT 5 cut(s) 246, 376, 700, 731, 1226
Alw21I GWGCWC 2 cut(s) 248, 857
Alw26I GTCTC 1 cut(s) 298
AlwI GGATC 7 cut(s) 112, 125, 542, 604, 859, 872, 965
AoxI GGCC 2 cut(s) 967, 1021
ApoI RAATTY 7 cut(s) 162, 289, 315, 498, 525, 791, 1087
AseI ATTAAT 2 cut(s) 805, 1136
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 2 cut(s) 799, 914
AsuC2I CCSGG 1 cut(s) 1082
AsuHPI GGTGA 3 cut(s) 49, 73, 211
AvaII GGWCC 2 cut(s) 799, 914
BamHI GGATCC 2 cut(s) 117, 864
BanI GGYRCC 1 cut(s) 1263
BanII GRGCYC 1 cut(s) 248
BbsI GAAGAC 2 cut(s) 289, 1010
Bbv12I GWGCWC 2 cut(s) 248, 857
BccI CCATC 8 cut(s) 28, 34, 58, 151, 174, 187, 223, 757
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 2 cut(s) 271, 1216
BciVI GTATCC 1 cut(s) 871
BcnI CCSGG 1 cut(s) 1082
BcoDI GTCTC 1 cut(s) 298
BfaI CTAG 4 cut(s) 253, 413, 491, 1163
BfrI CTTAAG 1 cut(s) 744
BfuI GTATCC 1 cut(s) 871
BglII AGATCT 1 cut(s) 1119
BisI GCNGC 1 cut(s) 1267
BlsI GCNGC 1 cut(s) 1268
Bme1390I CCNGG 3 cut(s) 271, 1082, 1216
Bme18I GGWCC 2 cut(s) 799, 914
BmgBI CACGTC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 799, 914
BmiI GGNNCC 5 cut(s) 119, 383, 437, 866, 1265
BmrFI CCNGG 3 cut(s) 271, 1082, 1216
BmrI ACTGGG 1 cut(s) 927
BmsI GCATC 3 cut(s) 246, 315, 594
BmuI ACTGGG 1 cut(s) 927
BpiI GAAGAC 2 cut(s) 289, 1010
BpmI CTGGAG 1 cut(s) 196
BpuEI CTTGAG 1 cut(s) 580
BpuMI CCSGG 1 cut(s) 1082
BsaAI YACGTR 1 cut(s) 171
BsaBI GATNNNNATC 4 cut(s) 116, 608, 713, 1043
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 6 cut(s) 222, 269, 270, 1081, 1214, 1234
Bsc4I CCNNNNNNNGG 4 cut(s) 619, 874, 938, 1030
Bse1I ACTGG 4 cut(s) 179, 933, 1012, 1201
Bse8I GATNNNNATC 4 cut(s) 116, 608, 713, 1043
BseBI CCWGG 2 cut(s) 271, 1216
BseDI CCNNGG 6 cut(s) 222, 269, 270, 1081, 1214, 1234
BseGI GGATG 6 cut(s) 36, 198, 330, 765, 784, 849
BseJI GATNNNNATC 4 cut(s) 116, 608, 713, 1043
BseLI CCNNNNNNNGG 4 cut(s) 619, 874, 938, 1030
BseMII CTCAG 2 cut(s) 136, 689
BseNI ACTGG 4 cut(s) 179, 933, 1012, 1201
BshFI GGCC 2 cut(s) 969, 1023
BshNI GGYRCC 1 cut(s) 1263
BsiHKAI GWGCWC 2 cut(s) 248, 857
BsiSI CCGG 2 cut(s) 541, 1081
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 4 cut(s) 619, 874, 938, 1030
BsmAI GTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 154
BsmI GAATGC 2 cut(s) 54, 264
BsnI GGCC 2 cut(s) 969, 1023
Bsp1286I GDGCHC 2 cut(s) 248, 857
Bsp1407I TGTACA 1 cut(s) 1112
Bsp143I GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
BspACI CCGC 3 cut(s) 912, 1126, 1267
BspANI GGCC 2 cut(s) 969, 1023
BspCNI CTCAG 2 cut(s) 135, 688
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 5 cut(s) 119, 383, 437, 866, 1265
BspPI GGATC 7 cut(s) 112, 125, 542, 604, 859, 872, 965
BspT107I GGYRCC 1 cut(s) 1263
BspTI CTTAAG 1 cut(s) 744
BsrGI TGTACA 1 cut(s) 1112
BsrI ACTGG 4 cut(s) 179, 933, 1012, 1201
BssECI CCNNGG 6 cut(s) 222, 269, 270, 1081, 1214, 1234
BssMI GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 222, 1234
Bst2UI CCWGG 2 cut(s) 271, 1216
Bst4CI ACNGT 3 cut(s) 650, 883, 907
Bst6I CTCTTC 3 cut(s) 349, 514, 977
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 744
BstAUI TGTACA 1 cut(s) 1112
BstBAI YACGTR 1 cut(s) 171
BstC8I GCNNGC 1 cut(s) 1025
BstDEI CTNAG 3 cut(s) 122, 675, 755
BstF5I GGATG 6 cut(s) 36, 198, 330, 765, 784, 849
BstKTI GATC 9 cut(s) 120, 252, 537, 612, 851, 867, 960, 1122, 1158
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
BstMWI GCNNNNNNNGC 1 cut(s) 1157
BstNI CCWGG 2 cut(s) 271, 1216
BstNSI RCATGY 1 cut(s) 1253
BstSCI CCNGG 3 cut(s) 269, 1080, 1214
BstSNI TACGTA 1 cut(s) 171
BstV2I GAAGAC 2 cut(s) 289, 1010
BstX2I RGATCY 5 cut(s) 117, 609, 864, 957, 1119
BstXI CCANNNNNNTGG 1 cut(s) 229
BstYI RGATCY 5 cut(s) 117, 609, 864, 957, 1119
BsuI GTATCC 1 cut(s) 871
BsuRI GGCC 2 cut(s) 969, 1023
BtgZI GCGATG 2 cut(s) 242, 1166
BtrI CACGTC 1 cut(s) 199
BtsCI GGATG 6 cut(s) 36, 198, 330, 765, 784, 849
BtsI GCAGTG 2 cut(s) 1075, 1148
BtsIMutI CAGTG 4 cut(s) 353, 917, 1075, 1148
Cac8I GCNNGC 1 cut(s) 1025
CciI TCATGA 1 cut(s) 1035
Cfr13I GGNCC 2 cut(s) 799, 914
CseI GACGC 1 cut(s) 87
Csp6I GTAC 4 cut(s) 172, 752, 884, 1113
CviAII CATG 5 cut(s) 76, 94, 871, 1036, 1250
CviJI RGCY 8 cut(s) 246, 376, 700, 731, 969, 1023, 1027, 1226
CviKI_1 RGCY 8 cut(s) 246, 376, 700, 731, 969, 1023, 1027, 1226
CviQI GTAC 4 cut(s) 172, 752, 884, 1113
DdeI CTNAG 3 cut(s) 122, 675, 755
DpnI GATC 9 cut(s) 119, 251, 536, 611, 850, 866, 959, 1121, 1157
DpnII GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
DraI TTTAAA 2 cut(s) 294, 1230
Eam1104I CTCTTC 3 cut(s) 349, 514, 977
EarI CTCTTC 3 cut(s) 349, 514, 977
Ecl136II GAGCTC 1 cut(s) 246
Eco105I TACGTA 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 222, 1234
Eco147I AGGCCT 1 cut(s) 969
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 799, 914
Eco53kI GAGCTC 1 cut(s) 246
EcoICRI GAGCTC 1 cut(s) 246
EcoRII CCWGG 2 cut(s) 269, 1214
EcoT14I CCWWGG 2 cut(s) 222, 1234
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 2 cut(s) 222, 1234
FaeI CATG 5 cut(s) 79, 97, 874, 1039, 1253
FaqI GGGAC 1 cut(s) 154
FatI CATG 5 cut(s) 75, 93, 870, 1035, 1249
FauI CCCGC 1 cut(s) 1119
FauNDI CATATG 1 cut(s) 1071
FblI GTMKAC 1 cut(s) 902
Fnu4HI GCNGC 1 cut(s) 1267
FokI GGATG 6 cut(s) 43, 205, 337, 771, 772, 856
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 1 cut(s) 1267
FspBI CTAG 4 cut(s) 253, 413, 491, 1163
GluI GCNGC 1 cut(s) 1267
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 2 cut(s) 969, 1023
HapII CCGG 2 cut(s) 541, 1081
HgaI GACGC 1 cut(s) 87
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 5 cut(s) 79, 97, 874, 1039, 1253
HindIII AAGCTT 1 cut(s) 1224
HinfI GANTC 5 cut(s) 90, 703, 709, 814, 838
HpaII CCGG 2 cut(s) 541, 1081
HphI GGTGA 3 cut(s) 49, 73, 211
Hpy166II GTNNAC 6 cut(s) 235, 418, 799, 886, 903, 917
Hpy188I TCNGA 5 cut(s) 678, 708, 819, 837, 843
Hpy188III TCNNGA 9 cut(s) 187, 253, 379, 559, 736, 788, 1036, 1105, 1292
Hpy8I GTNNAC 6 cut(s) 235, 418, 799, 886, 903, 917
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 4 cut(s) 144, 588, 1213, 1251
HpyCH4III ACNGT 3 cut(s) 650, 883, 907
HpyCH4IV ACGT 2 cut(s) 170, 198
HpyCH4V TGCA 5 cut(s) 607, 689, 954, 1075, 1141
HpyF10VI GCNNNNNNNGC 1 cut(s) 1157
HpyF3I CTNAG 3 cut(s) 122, 675, 755
HpySE526I ACGT 2 cut(s) 170, 198
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 5 cut(s) 79, 97, 874, 1039, 1253
Kzo9I GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
LweI GCATC 3 cut(s) 246, 315, 594
MaeI CTAG 4 cut(s) 253, 413, 491, 1163
MaeII ACGT 2 cut(s) 170, 198
MaeIII GTNAC 1 cut(s) 1143
MalI GATC 9 cut(s) 119, 251, 536, 611, 850, 866, 959, 1121, 1157
MboI GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
MflI RGATCY 5 cut(s) 117, 609, 864, 957, 1119
MhlI GDGCHC 2 cut(s) 248, 857
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 9 cut(s) 80, 293, 515, 745, 805, 962, 1136, 1171, 1229
MslI CAYNNNNRTG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 744
MspI CCGG 2 cut(s) 541, 1081
MspR9I CCNGG 3 cut(s) 271, 1082, 1216
Mva1269I GAATGC 2 cut(s) 54, 264
MvaI CCWGG 2 cut(s) 271, 1216
MwoI GCNNNNNNNGC 1 cut(s) 1157
NciI CCSGG 1 cut(s) 1082
NdeI CATATG 1 cut(s) 1071
NdeII GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
NlaIII CATG 5 cut(s) 79, 97, 874, 1039, 1253
NlaIV GGNNCC 5 cut(s) 119, 383, 437, 866, 1265
NmuCI GTSAC 1 cut(s) 1143
NspI RCATGY 1 cut(s) 1253
PagI TCATGA 1 cut(s) 1035
PasI CCCWGGG 1 cut(s) 270
PceI AGGCCT 1 cut(s) 969
PctI GAATGC 2 cut(s) 54, 264
PdmI GAANNNNTTC 1 cut(s) 498
PfeI GAWTC 5 cut(s) 90, 703, 709, 814, 838
PkrI GCNGC 1 cut(s) 1268
Ppu21I YACGTR 1 cut(s) 171
PshBI ATTAAT 2 cut(s) 805, 1136
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 269, 1214
PspGI CCWGG 2 cut(s) 269, 1214
PspN4I GGNNCC 5 cut(s) 119, 383, 437, 866, 1265
PspPI GGNCC 2 cut(s) 799, 914
PsuI RGATCY 5 cut(s) 117, 609, 864, 957, 1119
RsaI GTAC 4 cut(s) 173, 753, 885, 1114
RsaNI GTAC 4 cut(s) 172, 752, 884, 1113
RseI CAYNNNNRTG 1 cut(s) 59
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 9 cut(s) 80, 293, 515, 745, 805, 962, 1136, 1171, 1229
SatI GCNGC 1 cut(s) 1267
Sau3AI GATC 9 cut(s) 117, 249, 534, 609, 848, 864, 957, 1119, 1155
Sau96I GGNCC 2 cut(s) 799, 914
ScrFI CCNGG 3 cut(s) 271, 1082, 1216
SduI GDGCHC 2 cut(s) 248, 857
SfaNI GCATC 3 cut(s) 246, 315, 594
SinI GGWCC 2 cut(s) 799, 914
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 2 cut(s) 559, 744
SmoI CTYRAG 2 cut(s) 559, 744
SnaBI TACGTA 1 cut(s) 171
SseBI AGGCCT 1 cut(s) 969
SsiI CCGC 3 cut(s) 912, 1126, 1267
SspI AATATT 1 cut(s) 1209
SspMI CTAG 4 cut(s) 253, 413, 491, 1163
SstI GAGCTC 1 cut(s) 248
StuI AGGCCT 1 cut(s) 969
StyD4I CCNGG 3 cut(s) 269, 1080, 1214
StyI CCWWGG 2 cut(s) 222, 1234
TaaI ACNGT 3 cut(s) 650, 883, 907
TaiI ACGT 2 cut(s) 173, 201
TaqI TCGA 3 cut(s) 101, 248, 857
TatI WGTACW 2 cut(s) 751, 1112
TauI GCSGC 1 cut(s) 1269
TfiI GAWTC 5 cut(s) 90, 703, 709, 814, 838
Tru1I TTAA 9 cut(s) 80, 293, 515, 745, 805, 962, 1136, 1171, 1229
Tru9I TTAA 9 cut(s) 80, 293, 515, 745, 805, 962, 1136, 1171, 1229
TscAI CASTG 4 cut(s) 353, 924, 1082, 1148
TseFI GTSAC 1 cut(s) 1143
Tsp45I GTSAC 1 cut(s) 1143
TspDTI ATGAA 4 cut(s) 64, 155, 821, 836
TspGWI ACGGA 1 cut(s) 410
TspRI CASTG 4 cut(s) 353, 924, 1082, 1148
Vha464I CTTAAG 1 cut(s) 744
VpaK11BI GGWCC 2 cut(s) 799, 914
VspI ATTAAT 2 cut(s) 805, 1136
XapI RAATTY 7 cut(s) 162, 289, 315, 498, 525, 791, 1087
XbaI TCTAGA 1 cut(s) 252
XceI RCATGY 1 cut(s) 1253
XmiI GTMKAC 1 cut(s) 902
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 4 cut(s) 253, 413, 491, 1163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.