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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1720 |
| AccI | GTMKAC | 1 cut(s) | 1359 |
| AciI | CCGC | 4 cut(s) | 220, 1369, 1583, 1724 |
| AclWI | GGATC | 8 cut(s) | 456, 566, 579, 996, 1058, 1313, 1326, 1422 |
| AcsI | RAATTY | 9 cut(s) | 53, 105, 616, 743, 769, 952, 979, 1245, 1544 |
| AcyI | GRCGYC | 1 cut(s) | 552 |
| AfaI | GTAC | 6 cut(s) | 133, 310, 627, 1207, 1339, 1571 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 14, 181, 1073, 1328, 1395, 1487 |
| AflII | CTTAAG | 1 cut(s) | 1198 |
| AflIII | ACRYGT | 1 cut(s) | 652 |
| AjiI | CACGTC | 1 cut(s) | 653 |
| AjnI | CCWGG | 3 cut(s) | 383, 723, 1671 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 164, 196, 806, 838 |
| AleI | CACNNNNGTG | 1 cut(s) | 456 |
| AluBI | AGCT | 6 cut(s) | 98, 700, 830, 1154, 1185, 1683 |
| AluI | AGCT | 6 cut(s) | 98, 700, 830, 1154, 1185, 1683 |
| Alw21I | GWGCWC | 2 cut(s) | 702, 1311 |
| Alw26I | GTCTC | 1 cut(s) | 752 |
| AlwI | GGATC | 8 cut(s) | 456, 566, 579, 996, 1058, 1313, 1326, 1422 |
| AoxI | GGCC | 3 cut(s) | 5, 1424, 1478 |
| ApoI | RAATTY | 9 cut(s) | 53, 105, 616, 743, 769, 952, 979, 1245, 1544 |
| AseI | ATTAAT | 3 cut(s) | 261, 1259, 1593 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 952 |
| AspS9I | GGNCC | 3 cut(s) | 5, 1253, 1371 |
| AsuC2I | CCSGG | 2 cut(s) | 9, 1539 |
| AsuHPI | GGTGA | 7 cut(s) | 56, 62, 326, 356, 503, 527, 665 |
| AvaII | GGWCC | 2 cut(s) | 1253, 1371 |
| BamHI | GGATCC | 2 cut(s) | 571, 1318 |
| BanI | GGYRCC | 1 cut(s) | 1720 |
| BanII | GRGCYC | 1 cut(s) | 702 |
| BbsI | GAAGAC | 2 cut(s) | 743, 1467 |
| Bbv12I | GWGCWC | 2 cut(s) | 702, 1311 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 692, 726 |
| BciT130I | CCWGG | 3 cut(s) | 385, 725, 1673 |
| BciVI | GTATCC | 1 cut(s) | 1325 |
| BclI | TGATCA | 1 cut(s) | 250 |
| BcnI | CCSGG | 2 cut(s) | 9, 1539 |
| BcoDI | GTCTC | 1 cut(s) | 752 |
| BfaI | CTAG | 5 cut(s) | 246, 707, 867, 945, 1620 |
| BfrI | CTTAAG | 1 cut(s) | 1198 |
| BfuI | GTATCC | 1 cut(s) | 1325 |
| BglII | AGATCT | 1 cut(s) | 1576 |
| BisI | GCNGC | 1 cut(s) | 1724 |
| BlsI | GCNGC | 1 cut(s) | 1725 |
| Bme1390I | CCNGG | 5 cut(s) | 9, 385, 725, 1539, 1673 |
| Bme18I | GGWCC | 2 cut(s) | 1253, 1371 |
| BmgBI | CACGTC | 1 cut(s) | 653 |
| BmgT120I | GGNCC | 3 cut(s) | 5, 1253, 1371 |
| BmiI | GGNNCC | 6 cut(s) | 6, 573, 837, 891, 1320, 1722 |
| BmrFI | CCNGG | 5 cut(s) | 9, 385, 725, 1539, 1673 |
| BmrI | ACTGGG | 1 cut(s) | 1384 |
| BmsI | GCATC | 4 cut(s) | 370, 700, 769, 1048 |
| BmuI | ACTGGG | 1 cut(s) | 1384 |
| BpiI | GAAGAC | 2 cut(s) | 743, 1467 |
| BpmI | CTGGAG | 1 cut(s) | 650 |
| BpuEI | CTTGAG | 1 cut(s) | 1034 |
| BpuMI | CCSGG | 2 cut(s) | 9, 1539 |
| BsaAI | YACGTR | 1 cut(s) | 625 |
| BsaBI | GATNNNNATC | 4 cut(s) | 570, 1062, 1167, 1500 |
| BsaHI | GRCGYC | 1 cut(s) | 552 |
| BsaJI | CCNNGG | 7 cut(s) | 384, 676, 723, 724, 1538, 1671, 1691 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 76, 106 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 14, 181, 1073, 1328, 1395, 1487 |
| Bse1I | ACTGG | 5 cut(s) | 316, 633, 1390, 1469, 1658 |
| Bse8I | GATNNNNATC | 4 cut(s) | 570, 1062, 1167, 1500 |
| BseBI | CCWGG | 3 cut(s) | 385, 725, 1673 |
| BseDI | CCNNGG | 7 cut(s) | 384, 676, 723, 724, 1538, 1671, 1691 |
| BseGI | GGATG | 7 cut(s) | 400, 490, 652, 784, 1219, 1238, 1303 |
| BseJI | GATNNNNATC | 4 cut(s) | 570, 1062, 1167, 1500 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 14, 181, 1073, 1328, 1395, 1487 |
| BseMII | CTCAG | 4 cut(s) | 60, 197, 590, 1143 |
| BseNI | ACTGG | 5 cut(s) | 316, 633, 1390, 1469, 1658 |
| BseRI | GAGGAG | 1 cut(s) | 97 |
| BshFI | GGCC | 3 cut(s) | 7, 1426, 1480 |
| BshNI | GGYRCC | 1 cut(s) | 1720 |
| BsiHKAI | GWGCWC | 2 cut(s) | 702, 1311 |
| BsiSI | CCGG | 3 cut(s) | 8, 995, 1538 |
| BslFI | GGGAC | 1 cut(s) | 608 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 14, 181, 1073, 1328, 1395, 1487 |
| BsmAI | GTCTC | 1 cut(s) | 752 |
| BsmFI | GGGAC | 1 cut(s) | 608 |
| BsmI | GAATGC | 3 cut(s) | 358, 508, 718 |
| BsnI | GGCC | 3 cut(s) | 7, 1426, 1480 |
| Bsp1286I | GDGCHC | 2 cut(s) | 702, 1311 |
| Bsp1407I | TGTACA | 1 cut(s) | 1569 |
| BspACI | CCGC | 4 cut(s) | 220, 1369, 1583, 1724 |
| BspANI | GGCC | 3 cut(s) | 7, 1426, 1480 |
| BspCNI | CTCAG | 4 cut(s) | 61, 198, 589, 1142 |
| BspHI | TCATGA | 1 cut(s) | 1492 |
| BspLI | GGNNCC | 6 cut(s) | 6, 573, 837, 891, 1320, 1722 |
| BspPI | GGATC | 8 cut(s) | 456, 566, 579, 996, 1058, 1313, 1326, 1422 |
| BspT107I | GGYRCC | 1 cut(s) | 1720 |
| BspTI | CTTAAG | 1 cut(s) | 1198 |
| BsrGI | TGTACA | 1 cut(s) | 1569 |
| BsrI | ACTGG | 5 cut(s) | 316, 633, 1390, 1469, 1658 |
| BssECI | CCNNGG | 7 cut(s) | 384, 676, 723, 724, 1538, 1671, 1691 |
| BssNI | GRCGYC | 1 cut(s) | 552 |
| BssT1I | CCWWGG | 2 cut(s) | 676, 1691 |
| Bst2UI | CCWGG | 3 cut(s) | 385, 725, 1673 |
| Bst4CI | ACNGT | 7 cut(s) | 257, 344, 430, 455, 1104, 1337, 1364 |
| Bst6I | CTCTTC | 3 cut(s) | 803, 968, 1434 |
| BstACI | GRCGYC | 1 cut(s) | 552 |
| BstAFI | CTTAAG | 1 cut(s) | 1198 |
| BstAUI | TGTACA | 1 cut(s) | 1569 |
| BstBAI | YACGTR | 1 cut(s) | 625 |
| BstC8I | GCNNGC | 1 cut(s) | 1482 |
| BstDEI | CTNAG | 6 cut(s) | 69, 138, 206, 576, 1129, 1209 |
| BstF5I | GGATG | 7 cut(s) | 400, 490, 652, 784, 1219, 1238, 1303 |
| BstMAI | GTCTC | 1 cut(s) | 752 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1614 |
| BstNI | CCWGG | 3 cut(s) | 385, 725, 1673 |
| BstNSI | RCATGY | 1 cut(s) | 1710 |
| BstSCI | CCNGG | 5 cut(s) | 7, 383, 723, 1537, 1671 |
| BstSNI | TACGTA | 1 cut(s) | 625 |
| BstV2I | GAAGAC | 2 cut(s) | 743, 1467 |
| BstX2I | RGATCY | 5 cut(s) | 571, 1063, 1318, 1414, 1576 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 391, 683 |
| BstYI | RGATCY | 5 cut(s) | 571, 1063, 1318, 1414, 1576 |
| BsuI | GTATCC | 1 cut(s) | 1325 |
| BsuRI | GGCC | 3 cut(s) | 7, 1426, 1480 |
| BtgZI | GCGATG | 2 cut(s) | 696, 1623 |
| BtrI | CACGTC | 1 cut(s) | 653 |
| BtsCI | GGATG | 7 cut(s) | 400, 490, 652, 784, 1219, 1238, 1303 |
| BtsI | GCAGTG | 2 cut(s) | 1532, 1605 |
| BtsIMutI | CAGTG | 5 cut(s) | 460, 807, 1374, 1532, 1605 |
| Cac8I | GCNNGC | 1 cut(s) | 1482 |
| CciI | TCATGA | 1 cut(s) | 1492 |
| Cfr13I | GGNCC | 3 cut(s) | 5, 1253, 1371 |
| CseI | GACGC | 2 cut(s) | 113, 541 |
| Csp6I | GTAC | 6 cut(s) | 132, 309, 626, 1206, 1338, 1570 |
| CviAII | CATG | 5 cut(s) | 530, 548, 1325, 1493, 1707 |
| CviQI | GTAC | 6 cut(s) | 132, 309, 626, 1206, 1338, 1570 |
| DdeI | CTNAG | 6 cut(s) | 69, 138, 206, 576, 1129, 1209 |
| DraI | TTTAAA | 2 cut(s) | 748, 1687 |
| Eam1104I | CTCTTC | 3 cut(s) | 803, 968, 1434 |
| EarI | CTCTTC | 3 cut(s) | 803, 968, 1434 |
| Ecl136II | GAGCTC | 1 cut(s) | 700 |
| Eco105I | TACGTA | 1 cut(s) | 625 |
| Eco130I | CCWWGG | 2 cut(s) | 676, 1691 |
| Eco147I | AGGCCT | 1 cut(s) | 1426 |
| Eco24I | GRGCYC | 1 cut(s) | 702 |
| Eco47I | GGWCC | 2 cut(s) | 1253, 1371 |
| Eco53kI | GAGCTC | 1 cut(s) | 700 |
| EcoICRI | GAGCTC | 1 cut(s) | 700 |
| EcoRII | CCWGG | 3 cut(s) | 383, 723, 1671 |
| EcoT14I | CCWWGG | 2 cut(s) | 676, 1691 |
| EcoT38I | GRGCYC | 1 cut(s) | 702 |
| ErhI | CCWWGG | 2 cut(s) | 676, 1691 |
| FaeI | CATG | 5 cut(s) | 533, 551, 1328, 1496, 1710 |
| FaqI | GGGAC | 1 cut(s) | 608 |
| FatI | CATG | 5 cut(s) | 529, 547, 1324, 1492, 1706 |
| FauI | CCCGC | 2 cut(s) | 213, 1576 |
| FauNDI | CATATG | 1 cut(s) | 1528 |
| FbaI | TGATCA | 1 cut(s) | 250 |
| FblI | GTMKAC | 1 cut(s) | 1359 |
| Fnu4HI | GCNGC | 1 cut(s) | 1724 |
| FokI | GGATG | 7 cut(s) | 407, 497, 659, 791, 1225, 1226, 1310 |
| FriOI | GRGCYC | 1 cut(s) | 702 |
| Fsp4HI | GCNGC | 1 cut(s) | 1724 |
| FspBI | CTAG | 5 cut(s) | 246, 707, 867, 945, 1620 |
| GluI | GCNGC | 1 cut(s) | 1724 |
| GsuI | CTGGAG | 1 cut(s) | 650 |
| HaeIII | GGCC | 3 cut(s) | 7, 1426, 1480 |
| HapII | CCGG | 3 cut(s) | 8, 995, 1538 |
| HgaI | GACGC | 2 cut(s) | 113, 541 |
| Hin1I | GRCGYC | 1 cut(s) | 552 |
| Hin1II | CATG | 5 cut(s) | 533, 551, 1328, 1496, 1710 |
| HincII | GTYRAC | 1 cut(s) | 433 |
| HindII | GTYRAC | 1 cut(s) | 433 |
| HindIII | AAGCTT | 1 cut(s) | 1681 |
| HinfI | GANTC | 6 cut(s) | 214, 544, 1157, 1163, 1268, 1292 |
| HpaII | CCGG | 3 cut(s) | 8, 995, 1538 |
| HphI | GGTGA | 7 cut(s) | 56, 62, 326, 356, 503, 527, 665 |
| Hpy166II | GTNNAC | 8 cut(s) | 340, 433, 689, 872, 1253, 1340, 1360, 1374 |
| Hpy188I | TCNGA | 5 cut(s) | 1132, 1162, 1273, 1291, 1297 |
| Hpy8I | GTNNAC | 8 cut(s) | 340, 433, 689, 872, 1253, 1340, 1360, 1374 |
| Hpy99I | CGWCG | 2 cut(s) | 129, 557 |
| HpyAV | CCTTC | 5 cut(s) | 81, 598, 1042, 1670, 1708 |
| HpyCH4III | ACNGT | 7 cut(s) | 257, 344, 430, 455, 1104, 1337, 1364 |
| HpyCH4IV | ACGT | 2 cut(s) | 624, 652 |
| HpyCH4V | TGCA | 6 cut(s) | 161, 1061, 1143, 1411, 1532, 1598 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1614 |
| HpyF3I | CTNAG | 6 cut(s) | 69, 138, 206, 576, 1129, 1209 |
| HpySE526I | ACGT | 2 cut(s) | 624, 652 |
| Hsp92I | GRCGYC | 1 cut(s) | 552 |
| Hsp92II | CATG | 5 cut(s) | 533, 551, 1328, 1496, 1710 |
| Ksp22I | TGATCA | 1 cut(s) | 250 |
| LmnI | GCTCC | 1 cut(s) | 405 |
| LweI | GCATC | 4 cut(s) | 370, 700, 769, 1048 |
| MaeI | CTAG | 5 cut(s) | 246, 707, 867, 945, 1620 |
| MaeII | ACGT | 2 cut(s) | 624, 652 |
| MaeIII | GTNAC | 1 cut(s) | 1600 |
| MflI | RGATCY | 5 cut(s) | 571, 1063, 1318, 1414, 1576 |
| MhlI | GDGCHC | 2 cut(s) | 702, 1311 |
| MmeI | TCCRAC | 1 cut(s) | 162 |
| MroXI | GAANNNNTTC | 1 cut(s) | 952 |
| MslI | CAYNNNNRTG | 4 cut(s) | 246, 288, 456, 513 |
| MspA1I | CMGCKG | 1 cut(s) | 220 |
| MspCI | CTTAAG | 1 cut(s) | 1198 |
| MspI | CCGG | 3 cut(s) | 8, 995, 1538 |
| MspR9I | CCNGG | 5 cut(s) | 9, 385, 725, 1539, 1673 |
| Mva1269I | GAATGC | 3 cut(s) | 358, 508, 718 |
| MvaI | CCWGG | 3 cut(s) | 385, 725, 1673 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1614 |
| NciI | CCSGG | 2 cut(s) | 9, 1539 |
| NdeI | CATATG | 1 cut(s) | 1528 |
| NlaIII | CATG | 5 cut(s) | 533, 551, 1328, 1496, 1710 |
| NlaIV | GGNNCC | 6 cut(s) | 6, 573, 837, 891, 1320, 1722 |
| NmuCI | GTSAC | 1 cut(s) | 1600 |
| NspI | RCATGY | 1 cut(s) | 1710 |
| OliI | CACNNNNGTG | 1 cut(s) | 456 |
| PagI | TCATGA | 1 cut(s) | 1492 |
| PasI | CCCWGGG | 1 cut(s) | 724 |
| PceI | AGGCCT | 1 cut(s) | 1426 |
| PctI | GAATGC | 3 cut(s) | 358, 508, 718 |
| PdmI | GAANNNNTTC | 1 cut(s) | 952 |
| PfeI | GAWTC | 6 cut(s) | 214, 544, 1157, 1163, 1268, 1292 |
| PkrI | GCNGC | 1 cut(s) | 1725 |
| Ppu21I | YACGTR | 1 cut(s) | 625 |
| PshBI | ATTAAT | 3 cut(s) | 261, 1259, 1593 |
| Psp124BI | GAGCTC | 1 cut(s) | 702 |
| Psp6I | CCWGG | 3 cut(s) | 383, 723, 1671 |
| PspGI | CCWGG | 3 cut(s) | 383, 723, 1671 |
| PspN4I | GGNNCC | 6 cut(s) | 6, 573, 837, 891, 1320, 1722 |
| PspPI | GGNCC | 3 cut(s) | 5, 1253, 1371 |
| PsuI | RGATCY | 5 cut(s) | 571, 1063, 1318, 1414, 1576 |
| RsaI | GTAC | 6 cut(s) | 133, 310, 627, 1207, 1339, 1571 |
| RsaNI | GTAC | 6 cut(s) | 132, 309, 626, 1206, 1338, 1570 |
| RseI | CAYNNNNRTG | 4 cut(s) | 246, 288, 456, 513 |
| SacI | GAGCTC | 1 cut(s) | 702 |
| SatI | GCNGC | 1 cut(s) | 1724 |
| Sau96I | GGNCC | 3 cut(s) | 5, 1253, 1371 |
| ScrFI | CCNGG | 5 cut(s) | 9, 385, 725, 1539, 1673 |
| SduI | GDGCHC | 2 cut(s) | 702, 1311 |
| SfaNI | GCATC | 4 cut(s) | 370, 700, 769, 1048 |
| SinI | GGWCC | 2 cut(s) | 1253, 1371 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 246, 288, 456, 513 |
| SmlI | CTYRAG | 2 cut(s) | 1013, 1198 |
| SmoI | CTYRAG | 2 cut(s) | 1013, 1198 |
| SnaBI | TACGTA | 1 cut(s) | 625 |
| SseBI | AGGCCT | 1 cut(s) | 1426 |
| SsiI | CCGC | 4 cut(s) | 220, 1369, 1583, 1724 |
| SspI | AATATT | 1 cut(s) | 1666 |
| SspMI | CTAG | 5 cut(s) | 246, 707, 867, 945, 1620 |
| SstI | GAGCTC | 1 cut(s) | 702 |
| StuI | AGGCCT | 1 cut(s) | 1426 |
| StyD4I | CCNGG | 5 cut(s) | 7, 383, 723, 1537, 1671 |
| StyI | CCWWGG | 2 cut(s) | 676, 1691 |
| TaaI | ACNGT | 7 cut(s) | 257, 344, 430, 455, 1104, 1337, 1364 |
| TaiI | ACGT | 2 cut(s) | 627, 655 |
| TaqI | TCGA | 3 cut(s) | 555, 702, 1311 |
| TatI | WGTACW | 2 cut(s) | 1205, 1569 |
| TauI | GCSGC | 1 cut(s) | 1726 |
| TfiI | GAWTC | 6 cut(s) | 214, 544, 1157, 1163, 1268, 1292 |
| TscAI | CASTG | 5 cut(s) | 460, 807, 1381, 1539, 1605 |
| TseFI | GTSAC | 1 cut(s) | 1600 |
| Tsp45I | GTSAC | 1 cut(s) | 1600 |
| TspDTI | ATGAA | 7 cut(s) | 252, 294, 315, 518, 609, 1275, 1290 |
| TspGWI | ACGGA | 1 cut(s) | 864 |
| TspRI | CASTG | 5 cut(s) | 460, 807, 1381, 1539, 1605 |
| Vha464I | CTTAAG | 1 cut(s) | 1198 |
| VpaK11BI | GGWCC | 2 cut(s) | 1253, 1371 |
| VspI | ATTAAT | 3 cut(s) | 261, 1259, 1593 |
| XapI | RAATTY | 9 cut(s) | 53, 105, 616, 743, 769, 952, 979, 1245, 1544 |
| XbaI | TCTAGA | 1 cut(s) | 706 |
| XceI | RCATGY | 1 cut(s) | 1710 |
| XmiI | GTMKAC | 1 cut(s) | 1359 |
| XmnI | GAANNNNTTC | 1 cut(s) | 952 |
| XspI | CTAG | 5 cut(s) | 246, 707, 867, 945, 1620 |