MD08G1159900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000931
Physical Location & Seq
Forward (+)
18423368 .. 18427659
4292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1159900.v1.1.491

Sequence Viewer

Length: 2037 bp
ATGGCGCCAACTAAGTTGATAAGAAAGCTCAACAGTTTAAGGAAGGATTTTGAGGATGGTATGGGATCGGTGAGGTGCGGGAAGAGAGAGCGTGTTACTGAAAGGATATTTCAGCCCAATTCCTGCGGCAATGCCACAAAGAAAAGAAGAAAGTGCAGTATCCAAGTTGAAGGACAAGTACGGGATGAGGATTATGAGACATTCTTGATTTTTTTAGCTGACCATGGTAGTGATGTTGCTTATATTGACAATAAGTGGGTGGATGAACGGAATGATCATTCTGATGTCAACGTCATAAAAGATGATGATGGCAACGAAAATGGTATGTATGTTGGTGATGTTGGTGTCAACGTAAATGGTATGTCTGCAGATGATGTCGATGTCAATGCAAATGGTATGTCTGCGGATGATGTTGATGTCAATGCAAACGGTATGTCTTCAGATGACGCTGTTGTCAACGCAAATTGTATTTCTGTGGATGATGGTGATGTTAACGTAAAGGGTAGGTCTGTGGATGATGGCAATGACATCTTTGAGGTTAGGTCTTCAGATGATGGTCTTGCTGGTGATGGTGATGAGGATCCTGATTATAAGATACACTTGGATAAATTGACGAAAGGTGTAAAGTCCTGCATAAACAATAGGTCTGTGGATGATGATGATGATGTCCACATAAACGGTAGGTATGAGGATGATGGTGATGACAACGTTCATTTTATGTCTTTGGATGATGGTATTGATGATGAGGATCCTCAGTATAAGATATTCATGGAGAATTTGAGGCAAGATGGAAAGTCTTATGTACTGGAAGTTGTTGGAGATAATGGGAAATCAGAGCTGATCAAGTATGAAGAAGATGAGATACTAGACGGGACGGATTTTGACACCCCTGAAACTTTGAAAAAATCTCAGGTGAAAGAGAAGCCTGAAACTTTGAGAAGGGGTACGAAGAGAGAACAGATACATTGCCTTAAAAATTCTGCCCTTCCTGTGAATCGAGAAGTGACTGAGAGCCCAAGAACTGTTAGGTATGATATGAGGAAAGAAAACAGGGCAATTCTGGAAGATTCTGTTCCCAAGAAAAAGAGTTCAGTGCTGAAGAAGAATATAAAAGTGGTAGATCCGGGTCCTGTTTTCAATAGGACAGATGGACGTTTTAATAAATTGCCTAATTTAAAGGTTCCGTATACTCCAGAAACTTTCAAATATTCTCCAATGAAAAAGAAAACTGAACTTAGAAAAACCCCGAGTTTTGTGATAACGGGAAATGAAAGGAGCTTGATAAAGAAGAAGAAGAAGAATGTAGATAAGAAAAATGCAAATGGTAGCACAGGGGAGCGCACTATTAAAACCCCTCCTGTAGAGGCTGTTCAGTTTACCAAGCAAGGTTGTGCAAATCAGAAAGTTGTTAAAGATAGTACAAAGGAGAGTCCTCTTAAAAGATCTTGCATGGAGGGTCTTTGTTTTGCCAAGCAAGTATGCAACTGTGGATTAGCAGATGAAGACTATATAAAGGTTTTAAGTTGTCTACAATTTGTTGATGAGAAAATGCTATATTCACCTCCAAACGGGAGAACGGTGATTCTTGGTAATGATGGACAGAGTTCTCCGGATGTTGAAACGAAAGTGGAACCATCTGATAGAAATTTTACATCATTTGCAGCTGTGGATGCAGACAGTGGGCGGTGCGTTAAAACTCGTGATGCAAGTCATTCACAGTTTAGGAATGGGCTTATGAAGATTCTTAACAGGCCTTATGACTATGAAGAGTATAGAAGGCTCTTGAAAGAAGCGACTCACCAAAAGCCATTGGGGTACGACAGAAATCTGCGTAATGGAACCTGTCCAATAGAGGAGGAACTCAGACCTTCATATCTAAAACAGCACATAGTCCTGAAGAGAAAGATTAAGGCAGCTCTCGGTGACCGCCCCAGAGTTTTGAATCTCTTGCGGGGGTTTTTCTACTGGCTGAAGAATGTGGCCCAAGATGAAAGCTTCTGTCCATGGAATGACTCATCTTGTTTGAAAGTGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

679

Amino Acids

76.49

Weight (kDa)

5.69

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AasI GACNNNNNNGTC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 1187, 1528
AccIII TCCGGA 1 cut(s) 1609
AciI CCGC 6 cut(s) 78, 126, 404, 1684, 1927, 1951
AclI AACGTT 1 cut(s) 708
AclWI GGATC 6 cut(s) 73, 575, 588, 743, 756, 1115
AcsI RAATTY 3 cut(s) 775, 976, 1645
AcuI CTGAAG 5 cut(s) 423, 531, 1118, 1916, 1991
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 180, 804, 946, 1420, 1817
AfiI CCNNNNNNNGG 1 cut(s) 1568
AgsI TTSAA 8 cut(s) 170, 901, 1138, 1204, 1619, 1786, 1942, 2026
AluBI AGCT 7 cut(s) 28, 218, 838, 1278, 1664, 1916, 1995
AluI AGCT 7 cut(s) 28, 218, 838, 1278, 1664, 1916, 1995
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 6 cut(s) 73, 575, 588, 743, 756, 1115
Ama87I CYCGRG 1 cut(s) 1246
Aor13HI TCCGGA 1 cut(s) 1609
AoxI GGCC 2 cut(s) 1751, 1980
ApeKI GCWGC 2 cut(s) 1661, 1913
ApoI RAATTY 3 cut(s) 775, 976, 1645
ArsI GACNNNNNNTTYG 4 cut(s) 383, 415, 419, 451
AspLEI GCGC 2 cut(s) 7, 1341
AspS9I GGNCC 2 cut(s) 1127, 1981
AsuC2I CCSGG 1 cut(s) 1125
AvaI CYCGRG 1 cut(s) 1246
AvaII GGWCC 1 cut(s) 1127
BamHI GGATCC 2 cut(s) 580, 748
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1016
BarI GAAGNNNNNNTAC 4 cut(s) 162, 194, 846, 878
BauI CACGAG 1 cut(s) 1698
BbsI GAAGAC 3 cut(s) 429, 537, 1509
BbvI GCAGC 2 cut(s) 1673, 1925
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciVI GTATCC 1 cut(s) 170
BclI TGATCA 2 cut(s) 274, 840
BcnI CCSGG 1 cut(s) 1125
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 866
BfmI CTRYAG 2 cut(s) 366, 1359
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 1442
BisI GCNGC 3 cut(s) 127, 1662, 1914
BlsI GCNGC 3 cut(s) 128, 1663, 1915
Bme1390I CCNGG 1 cut(s) 1125
Bme18I GGWCC 1 cut(s) 1127
BmeT110I CYCGRG 1 cut(s) 1246
BmgT120I GGNCC 2 cut(s) 1127, 1981
BmiI GGNNCC 7 cut(s) 6, 582, 750, 1128, 1182, 1632, 1840
BmrFI CCNGG 1 cut(s) 1125
BmsI GCATC 2 cut(s) 1660, 1693
BpiI GAAGAC 3 cut(s) 429, 537, 1509
BpmI CTGGAG 1 cut(s) 1176
BpuMI CCSGG 1 cut(s) 1125
BsaBI GATNNNNATC 2 cut(s) 579, 747
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 223, 2003
BsaWI WCCGGW 1 cut(s) 1609
Bsc4I CCNNNNNNNGG 1 cut(s) 1568
Bse1I ACTGG 2 cut(s) 810, 1970
Bse3DI GCAATG 3 cut(s) 136, 529, 964
Bse8I GATNNNNATC 2 cut(s) 579, 747
BseAI TCCGGA 1 cut(s) 1609
BseDI CCNNGG 2 cut(s) 223, 2003
BseJI GATNNNNATC 2 cut(s) 579, 747
BseLI CCNNNNNNNGG 1 cut(s) 1568
BseMI GCAATG 3 cut(s) 136, 529, 964
BseMII CTCAG 4 cut(s) 767, 923, 999, 1876
BseNI ACTGG 2 cut(s) 810, 1970
BseRI GAGGAG 1 cut(s) 1868
BseXI GCAGC 2 cut(s) 1673, 1925
BsgI GTGCAG 1 cut(s) 175
BshFI GGCC 2 cut(s) 1753, 1982
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 1246
BsiSI CCGG 2 cut(s) 1124, 1610
BslFI GGGAC 1 cut(s) 886
BslI CCNNNNNNNGG 1 cut(s) 1568
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 886
BsnI GGCC 2 cut(s) 1753, 1982
BsoBI CYCGRG 1 cut(s) 1246
Bsp1286I GDGCHC 1 cut(s) 1016
Bsp13I TCCGGA 1 cut(s) 1609
Bsp143I GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
Bsp19I CCATGG 2 cut(s) 223, 2003
BspACI CCGC 6 cut(s) 78, 126, 404, 1684, 1927, 1951
BspANI GGCC 2 cut(s) 1753, 1982
BspCNI CTCAG 4 cut(s) 766, 922, 1000, 1875
BspEI TCCGGA 1 cut(s) 1609
BspLI GGNNCC 7 cut(s) 6, 582, 750, 1128, 1182, 1632, 1840
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 6 cut(s) 73, 575, 588, 743, 756, 1115
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 136, 529, 964
BsrI ACTGG 2 cut(s) 810, 1970
BssECI CCNNGG 2 cut(s) 223, 2003
BssMI GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
BssNAI GTATAC 1 cut(s) 1188
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 2 cut(s) 223, 2003
Bst1107I GTATAC 1 cut(s) 1188
Bst2BI CACGAG 1 cut(s) 1698
Bst4CI ACNGT 8 cut(s) 35, 431, 680, 1024, 1487, 1579, 1679, 1719
Bst6I CTCTTC 4 cut(s) 77, 944, 1761, 1892
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 6 cut(s) 12, 753, 909, 1008, 1235, 1862
BstDSI CCRYGG 2 cut(s) 223, 2003
BstEII GGTNACC 1 cut(s) 1922
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 1341
BstKTI GATC 7 cut(s) 68, 277, 583, 751, 843, 1123, 1445
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
BstMWI GCNNNNNNNGC 1 cut(s) 1670
BstPI GGTNACC 1 cut(s) 1922
BstSCI CCNGG 1 cut(s) 1123
BstSFI CTRYAG 2 cut(s) 366, 1359
BstV1I GCAGC 2 cut(s) 1673, 1925
BstV2I GAAGAC 3 cut(s) 429, 537, 1509
BstX2I RGATCY 4 cut(s) 580, 748, 1120, 1442
BstYI RGATCY 4 cut(s) 580, 748, 1120, 1442
BstZ17I GTATAC 1 cut(s) 1188
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 1753, 1982
BtgI CCRYGG 2 cut(s) 223, 2003
BtsIMutI CAGTG 2 cut(s) 1098, 1684
CfoI GCGC 2 cut(s) 7, 1341
Cfr13I GGNCC 2 cut(s) 1127, 1981
CseI GACGC 1 cut(s) 455
Csp6I GTAC 5 cut(s) 179, 803, 945, 1419, 1816
CviAII CATG 4 cut(s) 224, 769, 1450, 2004
CviQI GTAC 5 cut(s) 179, 803, 945, 1419, 1816
DdeI CTNAG 6 cut(s) 12, 753, 909, 1008, 1235, 1862
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 67, 276, 582, 750, 842, 1122, 1444
DpnII GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
DraI TTTAAA 1 cut(s) 1176
DrdI GACNNNNNNGTC 1 cut(s) 452
DseDI GACNNNNNNGTC 1 cut(s) 452
Eam1104I CTCTTC 4 cut(s) 77, 944, 1761, 1892
EarI CTCTTC 4 cut(s) 77, 944, 1761, 1892
Eco130I CCWWGG 2 cut(s) 223, 2003
Eco147I AGGCCT 1 cut(s) 1753
Eco24I GRGCYC 1 cut(s) 1016
Eco47I GGWCC 1 cut(s) 1127
Eco57I CTGAAG 5 cut(s) 423, 531, 1118, 1916, 1991
Eco88I CYCGRG 1 cut(s) 1246
Eco91I GGTNACC 1 cut(s) 1922
EcoO109I RGGNCCY 1 cut(s) 1127
EcoO65I GGTNACC 1 cut(s) 1922
EcoT14I CCWWGG 2 cut(s) 223, 2003
EcoT38I GRGCYC 1 cut(s) 1016
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 223, 2003
FaeI CATG 4 cut(s) 227, 772, 1453, 2007
FalI AAGNNNNNCTT 2 cut(s) 584, 616
FaqI GGGAC 1 cut(s) 886
FatI CATG 4 cut(s) 223, 768, 1449, 2003
FauI CCCGC 2 cut(s) 71, 1944
FbaI TGATCA 2 cut(s) 274, 840
FblI GTMKAC 2 cut(s) 1187, 1528
Fnu4HI GCNGC 3 cut(s) 127, 1662, 1914
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 3 cut(s) 127, 1662, 1914
FspBI CTAG 1 cut(s) 866
GlaI GCGC 2 cut(s) 6, 1340
GluI GCNGC 3 cut(s) 127, 1662, 1914
GsuI CTGGAG 1 cut(s) 1176
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 1753, 1982
HapII CCGG 2 cut(s) 1124, 1610
HgaI GACGC 1 cut(s) 455
HhaI GCGC 2 cut(s) 7, 1341
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 227, 772, 1453, 2007
Hin6I GCGC 2 cut(s) 5, 1339
HinP1I GCGC 2 cut(s) 5, 1339
HincII GTYRAC 4 cut(s) 289, 349, 457, 493
HindII GTYRAC 4 cut(s) 289, 349, 457, 493
HindIII AAGCTT 1 cut(s) 1993
HinfI GANTC 8 cut(s) 994, 1067, 1429, 1582, 1741, 1795, 1942, 2012
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 2 cut(s) 1124, 1610
Hpy166II GTNNAC 8 cut(s) 289, 349, 457, 493, 670, 1188, 1377, 1529
Hpy188I TCNGA 7 cut(s) 283, 442, 550, 835, 1401, 1639, 1865
Hpy188III TCNNGA 9 cut(s) 205, 584, 998, 1061, 1193, 1610, 1700, 1783, 1894
Hpy8I GTNNAC 8 cut(s) 289, 349, 457, 493, 670, 1188, 1377, 1529
HpyAV CCTTC 6 cut(s) 37, 164, 933, 995, 1770, 1878
HpyCH4III ACNGT 8 cut(s) 35, 431, 680, 1024, 1487, 1579, 1679, 1719
HpyCH4IV ACGT 5 cut(s) 291, 351, 495, 708, 1153
HpyF10VI GCNNNNNNNGC 1 cut(s) 1670
HpyF3I CTNAG 6 cut(s) 12, 753, 909, 1008, 1235, 1862
HpySE526I ACGT 5 cut(s) 291, 351, 495, 708, 1153
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 227, 772, 1453, 2007
HspAI GCGC 2 cut(s) 5, 1339
KasI GGCGCC 1 cut(s) 4
Kpn2I TCCGGA 1 cut(s) 1609
Ksp22I TGATCA 2 cut(s) 274, 840
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
LmnI GCTCC 2 cut(s) 1275, 1336
Lsp1109I GCAGC 2 cut(s) 1673, 1925
LweI GCATC 2 cut(s) 1660, 1693
MaeI CTAG 1 cut(s) 866
MaeII ACGT 5 cut(s) 291, 351, 495, 708, 1153
MaeIII GTNAC 4 cut(s) 94, 1003, 1922, 2029
MalI GATC 7 cut(s) 67, 276, 582, 750, 842, 1122, 1444
MboI GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
MflI RGATCY 4 cut(s) 580, 748, 1120, 1442
MhlI GDGCHC 1 cut(s) 1016
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 1438, 1789, 2006
MmeI TCCRAC 1 cut(s) 796
MroI TCCGGA 1 cut(s) 1609
MslI CAYNNNNRTG 2 cut(s) 228, 282
MspA1I CMGCKG 1 cut(s) 1664
MspI CCGG 2 cut(s) 1124, 1610
MspR9I CCNGG 1 cut(s) 1125
MwoI GCNNNNNNNGC 1 cut(s) 1670
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 1125
NcoI CCATGG 2 cut(s) 223, 2003
NdeII GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
NlaIII CATG 4 cut(s) 227, 772, 1453, 2007
NlaIV GGNNCC 7 cut(s) 6, 582, 750, 1128, 1182, 1632, 1840
NmuCI GTSAC 3 cut(s) 1003, 1922, 2029
PceI AGGCCT 1 cut(s) 1753
PfeI GAWTC 5 cut(s) 994, 1067, 1582, 1741, 1942
PkrI GCNGC 3 cut(s) 128, 1663, 1915
PleI GAGTC 3 cut(s) 1437, 1789, 2006
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 3 cut(s) 1437, 1789, 2006
PpuMI RGGWCCY 1 cut(s) 1127
PsiI TTATAA 1 cut(s) 591
Psp1406I AACGTT 1 cut(s) 708
Psp5II RGGWCCY 1 cut(s) 1127
PspEI GGTNACC 1 cut(s) 1922
PspN4I GGNNCC 7 cut(s) 6, 582, 750, 1128, 1182, 1632, 1840
PspPI GGNCC 2 cut(s) 1127, 1981
PspPPI RGGWCCY 1 cut(s) 1127
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 4 cut(s) 580, 748, 1120, 1442
PvuII CAGCTG 1 cut(s) 1664
RsaI GTAC 5 cut(s) 180, 804, 946, 1420, 1817
RsaNI GTAC 5 cut(s) 179, 803, 945, 1419, 1816
RseI CAYNNNNRTG 2 cut(s) 228, 282
SatI GCNGC 3 cut(s) 127, 1662, 1914
Sau3AI GATC 7 cut(s) 65, 274, 580, 748, 840, 1120, 1442
Sau96I GGNCC 2 cut(s) 1127, 1981
SchI GAGTC 3 cut(s) 1438, 1789, 2006
ScrFI CCNGG 1 cut(s) 1125
SduI GDGCHC 1 cut(s) 1016
SfaNI GCATC 2 cut(s) 1660, 1693
SfcI CTRYAG 2 cut(s) 366, 1359
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1127
SmiMI CAYNNNNRTG 2 cut(s) 228, 282
SseBI AGGCCT 1 cut(s) 1753
SsiI CCGC 6 cut(s) 78, 126, 404, 1684, 1927, 1951
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 1208
SspMI CTAG 1 cut(s) 866
StuI AGGCCT 1 cut(s) 1753
StyD4I CCNGG 1 cut(s) 1123
StyI CCWWGG 2 cut(s) 223, 2003
TaaI ACNGT 8 cut(s) 35, 431, 680, 1024, 1487, 1579, 1679, 1719
TaiI ACGT 5 cut(s) 294, 354, 498, 711, 1156
TaqI TCGA 2 cut(s) 378, 997
TatI WGTACW 2 cut(s) 802, 1418
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 5 cut(s) 994, 1067, 1582, 1741, 1942
TscAI CASTG 2 cut(s) 1098, 1684
TseFI GTSAC 3 cut(s) 1003, 1922, 2029
TseI GCWGC 2 cut(s) 1661, 1913
Tsp45I GTSAC 3 cut(s) 1003, 1922, 2029
TspGWI ACGGA 3 cut(s) 283, 890, 1173
TspRI CASTG 2 cut(s) 1098, 1684
VpaK11BI GGWCC 1 cut(s) 1127
XapI RAATTY 3 cut(s) 775, 976, 1645
XmiI GTMKAC 2 cut(s) 1187, 1528
XspI CTAG 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.