MD15G1133200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000931
Physical Location & Seq
Forward (+)
9659985 .. 9663564
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1133200.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGGCGCCAACTACATTGATAAGAGAACTCAACAGTTTAAAGAAGGATTTTGAGGACGGTATGGGTAAGGGATCAGTGAGGCGGGGGAAGAGAGAGCGTGTGGCCGATAGGATATTTGAGCCCAATTCCTGCGGCAATGCCACCAAGAAAAGAAGAAAGTGCAGTACTCAAGTTGAAGAACTGATACCGGATGAGGATTATGAGACATTCTTGAGTTCTTTAGCTGATCATGGCAGTGATGTTGATTATATTGACAATAGATGGGTGGATGATCGGAATGGTGGTGATGACAATGTAAATGATAGGTCTGTGGATGATGGCGATCACAGCGTTGAGGTAAGGTGTTTGGATGATGGTGTTGATGGTGGTGATGATGAGGATCCTGATCATAAGATACTCTTGGATAAATTGACAAAAGGTGGAAAGTCCTTCACAAACAATAGGTCTGTGGATGATGGTTATGATGATGATGATGATGTCCATGTAAACAGTAGGTATGCGGATGATGGTGATGAAAACATTCAGTCTCTGGATGATAGTCTTGATGATGAGGATCCTCAGTATAAGATATTCTTGGAAAAGTTGAGGCAAGATGGAAAGTCTTATGTGTTGGAAGCTGTTGGAGATAATGGGAAATCTGTAGTGATCAAGTATGAACAAGAAAATGAGATACTTAACGGGCCCGATTTTGACACCCCTGAAACATTTAAAAAATCTCAGGTGAAAGAGAAGCCTGATATTCAAAGCACTTTAAGGAGGGGTACGAAGAGAAAATGCACAAATTGCCCTAAAAATTCTGCTCTTCCTGTGTATAAAGAAGTGACTGCGAGCCCAAGAACTGTTAAGTATGATATGAGGAAAGAAAACAGGGCAATTCTGGAAAAGAGCTCGATGAAGAAGAAGAAGAAGAAGAAGAAGAATGTAAATGAGAAAGCTGCAAATGGTAGCACACGGGAGTGTCCTTTGAAAACACCCCCTGTGGAGGCTGTTCATTTTACCATGCAAGGATGTGCAAATGAGAAAGCTGCTAAAGGTAGCACAAAGGAGTGCCCTGTAAAAAGAACTCATGTGGCGGATCTCTGGTTTGCCAAACAAGGATGCAAGCGCGGATTAGAAGATGAGGACTACGCAAAGCTTTTAAGTTGTCTACAGTTTGATGATGGGAAAATGGTATATTCACCTCCAAATGGTAGACCGGTGATACTTGGTGATGATGGAGAGAGTTCACCTCCAAATAGAAGTTTTACCTCATTTACAGCTGTGGATGCAGACAGTGGGCGGTGCGTTAAAACTCGTGATGCAAGTCATTCGCAGTTTAGGAAGGGGCTTATGGATATTCTTACCAGGCCTTATGACCATGAAGAGTACAGAAGCCTCTTGAAAGAAGCAACTCACCAAAAGCCATTAGAGCACAACAGAAATCTGCGCGGTAGAACAATAACCTGTCCAGTTGAGGGGAAACTTGCACGTTCATATCTAAAACAGCACATAGTTCTGAAAAGAAAGATTACGGCAGCTCGCGGTGACCGCCCTAGAGTTTTGAATCTCTTGCGGGGATTTTTCTACTGGCTGAAGAATGTGGCCCAAGATGAAAGCTTCTGTCCTTGGAACGACTCGTCCTGTTTGAAAGTGCTGCCACCATCTGATAAGAAGACTGGACTTACAGAAAGGGGACTTGAAGGCAAACGCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

565

Amino Acids

63.76

Weight (kDa)

7.48

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 1147, 1192
AccII CGCG 3 cut(s) 1107, 1428, 1521
AclWI GGATC 6 cut(s) 79, 374, 387, 548, 561, 1083
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 793
AcuI CTGAAG 1 cut(s) 1592
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 166, 763, 1367
AfiI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
AgeI ACCGGT 1 cut(s) 1195
AgsI TTSAA 7 cut(s) 176, 743, 967, 1381, 1543, 1627, 1679
AjnI CCWGG 1 cut(s) 1343
AleI CACNNNNGTG 1 cut(s) 955
AloI GAACNNNNNNTCC 2 cut(s) 1601, 1633
AluBI AGCT 9 cut(s) 224, 617, 888, 935, 1025, 1135, 1259, 1517, 1596
AluI AGCT 9 cut(s) 224, 617, 888, 935, 1025, 1135, 1259, 1517, 1596
Alw21I GWGCWC 2 cut(s) 890, 1413
Alw26I GTCTC 2 cut(s) 197, 531
AlwI GGATC 6 cut(s) 79, 374, 387, 548, 561, 1083
AlwNI CAGNNNCTG 1 cut(s) 529
AoxI GGCC 4 cut(s) 102, 680, 1346, 1581
ApaI GGGCCC 1 cut(s) 684
ApeKI GCWGC 4 cut(s) 935, 1025, 1514, 1633
ApoI RAATTY 1 cut(s) 793
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
AsiGI ACCGGT 1 cut(s) 1195
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 3 cut(s) 7, 1107, 1428
AspS9I GGNCC 3 cut(s) 680, 681, 1582
BaeGI GKGCMC 2 cut(s) 684, 1052
BamHI GGATCC 2 cut(s) 379, 553
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 4 cut(s) 123, 684, 833, 890
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
BauI CACGAG 1 cut(s) 1293
BbsI GAAGAC 1 cut(s) 1658
Bbv12I GWGCWC 2 cut(s) 890, 1413
BbvI GCAGC 4 cut(s) 922, 1012, 1526, 1620
BceAI ACGGC 1 cut(s) 1527
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciT130I CCWGG 1 cut(s) 1345
BclI TGATCA 3 cut(s) 226, 385, 645
BcoDI GTCTC 2 cut(s) 197, 531
BfaI CTAG 1 cut(s) 1533
BfmI CTRYAG 2 cut(s) 639, 1148
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
BlsI GCNGC 5 cut(s) 134, 937, 1027, 1516, 1635
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 1345
BmgT120I GGNCC 3 cut(s) 680, 681, 1582
BmiI GGNNCC 4 cut(s) 6, 381, 555, 682
BmrFI CCNGG 1 cut(s) 1345
BmsI GCATC 3 cut(s) 1088, 1255, 1288
BpiI GAAGAC 1 cut(s) 1658
BplI GAGNNNNNCTC 2 cut(s) 1213, 1245
BpuEI CTTGAG 2 cut(s) 153, 232
BsaBI GATNNNNATC 4 cut(s) 321, 378, 384, 552
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 1604
BsaWI WCCGGW 2 cut(s) 187, 1195
Bsc4I CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
Bse118I RCCGGY 1 cut(s) 1195
Bse1I ACTGG 3 cut(s) 1448, 1571, 1660
Bse3DI GCAATG 1 cut(s) 142
Bse8I GATNNNNATC 4 cut(s) 321, 378, 384, 552
BseBI CCWGG 1 cut(s) 1345
BseDI CCNNGG 1 cut(s) 1604
BseJI GATNNNNATC 4 cut(s) 321, 378, 384, 552
BseLI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 572, 731
BseNI ACTGG 3 cut(s) 1448, 1571, 1660
BseSI GKGCMC 2 cut(s) 684, 1052
BseXI GCAGC 4 cut(s) 922, 1012, 1526, 1620
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 3 cut(s) 1107, 1428, 1521
BshFI GGCC 4 cut(s) 104, 682, 1348, 1583
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 1195
BsiHKAI GWGCWC 2 cut(s) 890, 1413
BsiSI CCGG 2 cut(s) 188, 1196
BslFI GGGAC 1 cut(s) 1686
BslI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
BsmAI GTCTC 2 cut(s) 197, 531
BsmFI GGGAC 1 cut(s) 1686
BsnI GGCC 4 cut(s) 104, 682, 1348, 1583
Bsp120I GGGCCC 1 cut(s) 680
Bsp1286I GDGCHC 6 cut(s) 123, 684, 833, 890, 1052, 1413
Bsp143I GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
BspANI GGCC 4 cut(s) 104, 682, 1348, 1583
BspCNI CTCAG 2 cut(s) 571, 730
BspFNI CGCG 3 cut(s) 1107, 1428, 1521
BspLI GGNNCC 4 cut(s) 6, 381, 555, 682
BspPI GGATC 6 cut(s) 79, 374, 387, 548, 561, 1083
BspQI GCTCTTC 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 142
BsrFI RCCGGY 1 cut(s) 1195
BsrI ACTGG 3 cut(s) 1448, 1571, 1660
BssAI RCCGGY 1 cut(s) 1195
BssECI CCNNGG 1 cut(s) 1604
BssMI GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1293
BssT1I CCWWGG 1 cut(s) 1604
Bst2BI CACGAG 1 cut(s) 1293
Bst2UI CCWGG 1 cut(s) 1345
Bst4CI ACNGT 6 cut(s) 35, 59, 491, 841, 1152, 1274
Bst6I CTCTTC 4 cut(s) 83, 761, 807, 1356
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 783
BstC8I GCNNGC 3 cut(s) 829, 1103, 1519
BstDEI CTNAG 2 cut(s) 558, 717
BstEII GGTNACC 1 cut(s) 1523
BstFNI CGCG 3 cut(s) 1107, 1428, 1521
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 1107, 1428
BstKTI GATC 9 cut(s) 74, 229, 274, 325, 382, 388, 556, 648, 1078
BstMAI GTCTC 2 cut(s) 197, 531
BstMBI GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
BstMWI GCNNNNNNNGC 5 cut(s) 327, 783, 1265, 1408, 1527
BstNI CCWGG 1 cut(s) 1345
BstPI GGTNACC 1 cut(s) 1523
BstSCI CCNGG 1 cut(s) 1343
BstSFI CTRYAG 2 cut(s) 639, 1148
BstSLI GKGCMC 2 cut(s) 684, 1052
BstUI CGCG 3 cut(s) 1107, 1428, 1521
BstV1I GCAGC 4 cut(s) 922, 1012, 1526, 1620
BstV2I GAAGAC 1 cut(s) 1658
BstX2I RGATCY 3 cut(s) 379, 553, 1075
BstYI RGATCY 3 cut(s) 379, 553, 1075
BsuRI GGCC 4 cut(s) 104, 682, 1348, 1583
BtsI GCAGTG 1 cut(s) 241
BtsIMutI CAGTG 3 cut(s) 81, 241, 1279
Cac8I GCNNGC 3 cut(s) 829, 1103, 1519
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 3 cut(s) 7, 1107, 1428
Cfr10I RCCGGY 1 cut(s) 1195
Cfr13I GGNCC 3 cut(s) 680, 681, 1582
Csp6I GTAC 3 cut(s) 165, 762, 1366
CspAI ACCGGT 1 cut(s) 1195
CviAII CATG 5 cut(s) 230, 482, 1000, 1067, 1358
CviQI GTAC 3 cut(s) 165, 762, 1366
DdeI CTNAG 2 cut(s) 558, 717
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 73, 228, 273, 324, 381, 387, 555, 647, 1077
DpnII GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
DraI TTTAAA 2 cut(s) 39, 709
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 4 cut(s) 83, 761, 807, 1356
EarI CTCTTC 4 cut(s) 83, 761, 807, 1356
EciI GGCGGA 1 cut(s) 1088
Ecl136II GAGCTC 1 cut(s) 888
Eco130I CCWWGG 1 cut(s) 1604
Eco147I AGGCCT 1 cut(s) 1348
Eco24I GRGCYC 4 cut(s) 123, 684, 833, 890
Eco53kI GAGCTC 1 cut(s) 888
Eco57I CTGAAG 1 cut(s) 1592
Eco91I GGTNACC 1 cut(s) 1523
EcoICRI GAGCTC 1 cut(s) 888
EcoO65I GGTNACC 1 cut(s) 1523
EcoRII CCWGG 1 cut(s) 1343
EcoT14I CCWWGG 1 cut(s) 1604
EcoT38I GRGCYC 4 cut(s) 123, 684, 833, 890
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 1604
FaeI CATG 5 cut(s) 233, 485, 1003, 1070, 1361
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 557, 589
FaqI GGGAC 1 cut(s) 1686
FatI CATG 5 cut(s) 229, 481, 999, 1066, 1357
FauI CCCGC 2 cut(s) 75, 1545
FbaI TGATCA 3 cut(s) 226, 385, 645
FblI GTMKAC 2 cut(s) 1147, 1192
Fnu4HI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
FriOI GRGCYC 4 cut(s) 123, 684, 833, 890
Fsp4HI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
FspBI CTAG 1 cut(s) 1533
GlaI GCGC 3 cut(s) 6, 1106, 1427
GluI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 104, 682, 1348, 1583
HapII CCGG 2 cut(s) 188, 1196
HhaI GCGC 3 cut(s) 7, 1107, 1428
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 233, 485, 1003, 1070, 1361
Hin6I GCGC 3 cut(s) 5, 1105, 1426
HinP1I GCGC 3 cut(s) 5, 1105, 1426
HindIII AAGCTT 2 cut(s) 1133, 1594
HinfI GANTC 2 cut(s) 1543, 1613
HpaII CCGG 2 cut(s) 188, 1196
Hpy166II GTNNAC 4 cut(s) 487, 1148, 1193, 1226
Hpy188I TCNGA 3 cut(s) 276, 1497, 1645
Hpy188III TCNNGA 7 cut(s) 211, 383, 530, 542, 878, 1295, 1378
Hpy8I GTNNAC 4 cut(s) 487, 1148, 1193, 1226
HpyAV CCTTC 4 cut(s) 37, 439, 1315, 1673
HpyCH4III ACNGT 6 cut(s) 35, 59, 491, 841, 1152, 1274
HpyCH4IV ACGT 1 cut(s) 1468
HpyCH4V TGCA 9 cut(s) 162, 777, 938, 1003, 1013, 1101, 1268, 1301, 1466
HpyF10VI GCNNNNNNNGC 5 cut(s) 327, 783, 1265, 1408, 1527
HpyF3I CTNAG 2 cut(s) 558, 717
HpySE526I ACGT 1 cut(s) 1468
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 233, 485, 1003, 1070, 1361
HspAI GCGC 3 cut(s) 5, 1105, 1426
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 3 cut(s) 226, 385, 645
Kzo9I GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
LguI GCTCTTC 1 cut(s) 807
Lsp1109I GCAGC 4 cut(s) 922, 1012, 1526, 1620
LweI GCATC 3 cut(s) 1088, 1255, 1288
MaeI CTAG 1 cut(s) 1533
MaeII ACGT 1 cut(s) 1468
MaeIII GTNAC 2 cut(s) 820, 1523
MalI GATC 9 cut(s) 73, 228, 273, 324, 381, 387, 555, 647, 1077
MboI GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
MflI RGATCY 3 cut(s) 379, 553, 1075
MhlI GDGCHC 6 cut(s) 123, 684, 833, 890, 1052, 1413
MluCI AATT 5 cut(s) 124, 407, 781, 793, 873
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1607
MmeI TCCRAC 2 cut(s) 591, 601
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 7 cut(s) 38, 675, 708, 752, 843, 1139, 1287
MslI CAYNNNNRTG 2 cut(s) 234, 955
MspA1I CMGCKG 1 cut(s) 1259
MspI CCGG 2 cut(s) 188, 1196
MspR9I CCNGG 1 cut(s) 1345
MvaI CCWGG 1 cut(s) 1345
MvnI CGCG 3 cut(s) 1107, 1428, 1521
MwoI GCNNNNNNNGC 5 cut(s) 327, 783, 1265, 1408, 1527
NarI GGCGCC 1 cut(s) 5
NdeII GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
NlaIII CATG 5 cut(s) 233, 485, 1003, 1070, 1361
NlaIV GGNNCC 4 cut(s) 6, 381, 555, 682
NmuCI GTSAC 2 cut(s) 820, 1523
OliI CACNNNNGTG 1 cut(s) 955
PceI AGGCCT 1 cut(s) 1348
PciSI GCTCTTC 1 cut(s) 807
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 1543
PinAI ACCGGT 1 cut(s) 1195
PkrI GCNGC 5 cut(s) 134, 937, 1027, 1516, 1635
PleI GAGTC 1 cut(s) 1607
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 1607
Psp124BI GAGCTC 1 cut(s) 890
Psp6I CCWGG 1 cut(s) 1343
PspEI GGTNACC 1 cut(s) 1523
PspGI CCWGG 1 cut(s) 1343
PspN4I GGNNCC 4 cut(s) 6, 381, 555, 682
PspOMI GGGCCC 1 cut(s) 680
PspPI GGNCC 3 cut(s) 680, 681, 1582
PstNI CAGNNNCTG 1 cut(s) 529
PsuI RGATCY 3 cut(s) 379, 553, 1075
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 3 cut(s) 166, 763, 1367
RsaNI GTAC 3 cut(s) 165, 762, 1366
RseI CAYNNNNRTG 2 cut(s) 234, 955
SacI GAGCTC 1 cut(s) 890
SapI GCTCTTC 1 cut(s) 807
SaqAI TTAA 7 cut(s) 38, 675, 708, 752, 843, 1139, 1287
SatI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
Sau3AI GATC 9 cut(s) 71, 226, 271, 322, 379, 385, 553, 645, 1075
Sau96I GGNCC 3 cut(s) 680, 681, 1582
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 1 cut(s) 1607
ScrFI CCNGG 1 cut(s) 1345
SduI GDGCHC 6 cut(s) 123, 684, 833, 890, 1052, 1413
SfaNI GCATC 3 cut(s) 1088, 1255, 1288
SfcI CTRYAG 2 cut(s) 639, 1148
SfoI GGCGCC 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 234, 955
SmlI CTYRAG 2 cut(s) 168, 211
SmoI CTYRAG 2 cut(s) 168, 211
Sse9I AATT 5 cut(s) 124, 407, 781, 793, 873
SseBI AGGCCT 1 cut(s) 1348
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 1533
SstI GAGCTC 1 cut(s) 890
StuI AGGCCT 1 cut(s) 1348
StyD4I CCNGG 1 cut(s) 1343
StyI CCWWGG 1 cut(s) 1604
TaaI ACNGT 6 cut(s) 35, 59, 491, 841, 1152, 1274
TaiI ACGT 1 cut(s) 1471
TaqI TCGA 1 cut(s) 890
TasI AATT 5 cut(s) 124, 407, 781, 793, 873
TatI WGTACW 2 cut(s) 164, 1365
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 1543
Tru1I TTAA 7 cut(s) 38, 675, 708, 752, 843, 1139, 1287
Tru9I TTAA 7 cut(s) 38, 675, 708, 752, 843, 1139, 1287
TscAI CASTG 3 cut(s) 81, 241, 1279
TseFI GTSAC 2 cut(s) 820, 1523
TseI GCWGC 4 cut(s) 935, 1025, 1514, 1633
Tsp45I GTSAC 2 cut(s) 820, 1523
TspDTI ATGAA 7 cut(s) 528, 669, 908, 980, 1374, 1461, 1605
TspRI CASTG 3 cut(s) 81, 241, 1279
XapI RAATTY 1 cut(s) 793
XmiI GTMKAC 2 cut(s) 1147, 1192
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 1 cut(s) 1533
ZrmI AGTACT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.