Prupe.3G258400_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000931
Physical Location & Seq
Reverse (-)
24546821 .. 24550870
4050 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G258400.1

Sequence Viewer

Length: 1452 bp
ATGAGGTTGGCGAGGTGGAGGAAGAGAAAGTGCGTTGCTGATGATAGGAATTATGAGCCCAATTCCTGGATCAGTGCCACGAAGAAAAGAAGAAACTATGCTAATACTATCCAAGTGGACGAAGAAATACTGGATAAGGAGTATGAGGTTTTTCTTGAGGGGTTAGAACTGCATGGTGGTGATGATGGTCAAGATTATCATGACACTAGGTGGATAGTTGATGGGGATGTTCATTCTAATGCTCAGTTGATGGATCGTGGTGATGTCAACCTAAATGGTAGGTCTGTGGATAGCGATGATTTTGATGATGATGTGGATCCTCAGTATAAGATATTATTGGAAAATCTGAAGGAAGATGGAAAGTCCTATGTATTGGTGCTTGTTCGAGAGAATGAGAATTTGGAGCTGATAAAGTATGAACAAGAAGATGGGGAACTAGACGAGACCAATTTAGATACCCCAGAAACTGTGAAAAAATCTGATGAGGAAATCTTTGTCAAACCCATTAGGATTTGCAAAAGAAAGACAATTCACCCCTGTCCCACATCAATATCTCATGTGAAAGAGAAGACTGAAATAAAAAAAGCTAGCCCAAGAACTGTTAGGTATGCTATGAGGAAAAAAAAAAGGGGAATTCTGGAAGATTCAGAGGGTCTGCCAAATAAAAAGAGTTTAGGGGTGAAGAAGAATGTAAAAGTGGAAGCTCTGGATCCTGTTTCTGATAGGACAAAAGGGCGTTCTAATAAAATGCATGGTGTAGAGGGTCCCACTACTCGAGAAACTTTAAAAAGTTCTCATGTGAAGAAGAAGAAAGTAAATAAGAAAGGTGCAGATAGTAGGACAAAGGGGCATCCTGTTAAAAGACCTCATTTGGCCGAGCATGGACACAACCATGGAGCTGTGTCTGATCAAATAGACTTGGACTATCAAGAGGTTCTAGATGGTCTAAGAAAATACGGTGGAAAGTGGGTGTATACACCTACAACTGCTGGACCAGTGGCATGTGTACTTGAGGAGGATGTGGAGAGTTCAGCTGTAGAAATTAAGAAAGAACCATGTGATGAGTATTTTACCTCATCTACAGCTGTGGGCGTAGACGGTGGATGGTGCGTTGAAACTTGTGATACAAGTCATGCCCAGTTTAGGAAGGGGCTTATGAAGGACCTTAAACGGCCTTATGACGAAAAAGAGTGTAAAAGGCTCTTGAAACAACTGAACCGACGAAGGTCAGTGTCAGGGAAATTAAGGGCTTCATATCGAAAGCAGCACATAGTCCTAGCAAGAAAGCTTGATGCTGCTAGCAGCGACTGCCCTAGAATTTTGAATCTTTTGCGTGGATTTTTCTACTGGCTGAAGAATGTGGCCCAAGAAGGAAGCTTCTGTCCTTGGAAGGACTCGTCTTGTTTGAAGGTGTTGCCTCAAGTTGGAAGGAAGTTTAGCTACATGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

484

Amino Acids

55.26

Weight (kDa)

8.84

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 2 cut(s) 974, 1095
AclWI GGATC 6 cut(s) 77, 261, 311, 324, 704, 717
AcoI YGGCCR 1 cut(s) 873
AcsI RAATTY 3 cut(s) 397, 633, 1317
AcuI CTGAAG 2 cut(s) 368, 1373
AdeI CACNNNGTG 1 cut(s) 210
AfaI GTAC 1 cut(s) 1008
AfiI CCNNNNNNNGG 3 cut(s) 66, 1143, 1424
AgsI TTSAA 4 cut(s) 1115, 1207, 1324, 1408
AjnI CCWGG 1 cut(s) 65
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 9 cut(s) 406, 587, 704, 899, 1034, 1085, 1288, 1377, 1440
AluI AGCT 9 cut(s) 406, 587, 704, 899, 1034, 1085, 1288, 1377, 1440
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 6 cut(s) 77, 261, 311, 324, 704, 717
AlwNI CAGNNNCTG 2 cut(s) 467, 1308
Ama87I CYCGRG 1 cut(s) 774
AoxI GGCC 3 cut(s) 873, 1172, 1362
ApeKI GCWGC 3 cut(s) 1264, 1295, 1302
ApoI RAATTY 3 cut(s) 397, 633, 1317
AspS9I GGNCC 4 cut(s) 764, 992, 1162, 1363
AsuHPI GGTGA 4 cut(s) 191, 272, 524, 691
AsuNHI GCTAGC 2 cut(s) 587, 1298
AvaI CYCGRG 1 cut(s) 774
AvaII GGWCC 3 cut(s) 764, 992, 1162
BamHI GGATCC 2 cut(s) 316, 709
BanII GRGCYC 1 cut(s) 60
BarI GAAGNNNNNNTAC 1 cut(s) 1424
BbsI GAAGAC 1 cut(s) 575
BbvI GCAGC 3 cut(s) 1276, 1282, 1314
BccI CCATC 7 cut(s) 179, 215, 244, 350, 422, 935, 1098
BceAI ACGGC 1 cut(s) 1187
BciT130I CCWGG 1 cut(s) 67
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 7 cut(s) 207, 437, 588, 938, 1277, 1299, 1314
BfmI CTRYAG 2 cut(s) 1035, 1080
BisI GCNGC 3 cut(s) 1265, 1296, 1303
BlsI GCNGC 3 cut(s) 1266, 1297, 1304
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 3 cut(s) 764, 992, 1162
BmeT110I CYCGRG 1 cut(s) 774
BmgT120I GGNCC 4 cut(s) 764, 992, 1162, 1363
BmiI GGNNCC 4 cut(s) 318, 711, 765, 766
BmrFI CCNGG 1 cut(s) 67
BmrI ACTGGG 1 cut(s) 1132
BmsI GCATC 2 cut(s) 859, 1282
BmtI GCTAGC 2 cut(s) 591, 1302
BmuI ACTGGG 1 cut(s) 1132
BoxI GACNNNNGTC 1 cut(s) 1225
BpiI GAAGAC 1 cut(s) 575
BpuEI CTTGAG 3 cut(s) 176, 1031, 1404
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 892, 1385
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 1143, 1424
Bse1I ACTGG 4 cut(s) 135, 995, 1138, 1352
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 67
BseDI CCNNGG 2 cut(s) 892, 1385
BseGI GGATG 4 cut(s) 232, 850, 1024, 1109
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 3 cut(s) 66, 1143, 1424
BseMII CTCAG 2 cut(s) 257, 335
BseNI ACTGG 4 cut(s) 135, 995, 1138, 1352
BseRI GAGGAG 1 cut(s) 1028
BseXI GCAGC 3 cut(s) 1276, 1282, 1314
BsgI GTGCAG 1 cut(s) 849
BshFI GGCC 3 cut(s) 875, 1174, 1364
BsiHKCI CYCGRG 1 cut(s) 774
BslFI GGGAC 2 cut(s) 525, 750
BslI CCNNNNNNNGG 3 cut(s) 66, 1143, 1424
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 525, 750
BsnI GGCC 3 cut(s) 875, 1174, 1364
Bso31I GGTCTC 1 cut(s) 437
BsoBI CYCGRG 1 cut(s) 774
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 5 cut(s) 69, 253, 316, 709, 907
Bsp19I CCATGG 1 cut(s) 892
BspANI GGCC 3 cut(s) 875, 1174, 1364
BspCNI CTCAG 2 cut(s) 256, 334
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 4 cut(s) 318, 711, 765, 766
BspOI GCTAGC 2 cut(s) 591, 1302
BspPI GGATC 6 cut(s) 77, 261, 311, 324, 704, 717
BspTNI GGTCTC 1 cut(s) 437
BsrI ACTGG 4 cut(s) 135, 995, 1138, 1352
BssECI CCNNGG 2 cut(s) 892, 1385
BssMI GATC 5 cut(s) 69, 253, 316, 709, 907
BssNAI GTATAC 1 cut(s) 975
BssT1I CCWWGG 2 cut(s) 892, 1385
Bst1107I GTATAC 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 4 cut(s) 469, 601, 959, 1100
Bst6I CTCTTC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 1308
BstC8I GCNNGC 2 cut(s) 589, 1300
BstDEI CTNAG 3 cut(s) 243, 321, 947
BstDSI CCRYGG 1 cut(s) 892
BstF5I GGATG 4 cut(s) 232, 850, 1024, 1109
BstKTI GATC 5 cut(s) 72, 256, 319, 712, 910
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 5 cut(s) 69, 253, 316, 709, 907
BstMWI GCNNNNNNNGC 1 cut(s) 1308
BstNI CCWGG 1 cut(s) 67
BstNSI RCATGY 1 cut(s) 1005
BstPAI GACNNNNGTC 1 cut(s) 1225
BstSCI CCNGG 1 cut(s) 65
BstSFI CTRYAG 2 cut(s) 1035, 1080
BstV1I GCAGC 3 cut(s) 1276, 1282, 1314
BstV2I GAAGAC 1 cut(s) 575
BstX2I RGATCY 2 cut(s) 316, 709
BstYI RGATCY 2 cut(s) 316, 709
BstZ17I GTATAC 1 cut(s) 975
BsuRI GGCC 3 cut(s) 875, 1174, 1364
BtgI CCRYGG 1 cut(s) 892
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 4 cut(s) 232, 850, 1024, 1109
BtsIMutI CAGTG 3 cut(s) 79, 1002, 1236
Cac8I GCNNGC 2 cut(s) 589, 1300
CaiI CAGNNNCTG 2 cut(s) 467, 1308
CciI TCATGA 1 cut(s) 199
Cfr13I GGNCC 4 cut(s) 764, 992, 1162, 1363
Csp6I GTAC 1 cut(s) 1007
CviQI GTAC 1 cut(s) 1007
DdeI CTNAG 3 cut(s) 243, 321, 947
DpnI GATC 5 cut(s) 71, 255, 318, 711, 909
DpnII GATC 5 cut(s) 69, 253, 316, 709, 907
DraI TTTAAA 1 cut(s) 786
DraIII CACNNNGTG 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 873
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 892, 1385
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 437
Eco47I GGWCC 3 cut(s) 764, 992, 1162
Eco57I CTGAAG 2 cut(s) 368, 1373
Eco88I CYCGRG 1 cut(s) 774
EcoO109I RGGNCCY 2 cut(s) 764, 1162
EcoRI GAATTC 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 65
EcoT14I CCWWGG 2 cut(s) 892, 1385
EcoT22I ATGCAT 1 cut(s) 753
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 2 cut(s) 892, 1385
FaqI GGGAC 2 cut(s) 525, 750
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 2 cut(s) 974, 1095
Fnu4HI GCNGC 3 cut(s) 1265, 1296, 1303
FokI GGATG 4 cut(s) 239, 837, 1031, 1116
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 3 cut(s) 1265, 1296, 1303
FspBI CTAG 7 cut(s) 207, 437, 588, 938, 1277, 1299, 1314
GluI GCNGC 3 cut(s) 1265, 1296, 1303
HaeIII GGCC 3 cut(s) 875, 1174, 1364
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 1286, 1375
HinfI GANTC 3 cut(s) 644, 1324, 1394
HphI GGTGA 4 cut(s) 191, 272, 524, 691
Hpy166II GTNNAC 5 cut(s) 118, 268, 975, 1007, 1096
Hpy188I TCNGA 5 cut(s) 348, 481, 649, 721, 907
Hpy8I GTNNAC 5 cut(s) 118, 268, 975, 1007, 1096
Hpy99I CGWCG 1 cut(s) 1224
HpyAV CCTTC 8 cut(s) 343, 1141, 1153, 1218, 1364, 1384, 1402, 1422
HpyCH4III ACNGT 4 cut(s) 469, 601, 959, 1100
HpyCH4V TGCA 4 cut(s) 172, 516, 751, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 1308
HpyF3I CTNAG 3 cut(s) 243, 321, 947
KflI GGGWCCC 1 cut(s) 764
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 5 cut(s) 69, 253, 316, 709, 907
LmnI GCTCC 2 cut(s) 403, 896
Lsp1109I GCAGC 3 cut(s) 1276, 1282, 1314
LweI GCATC 2 cut(s) 859, 1282
MaeI CTAG 7 cut(s) 207, 437, 588, 938, 1277, 1299, 1314
MalI GATC 5 cut(s) 71, 255, 318, 711, 909
MboI GATC 5 cut(s) 69, 253, 316, 709, 907
MflI RGATCY 2 cut(s) 316, 709
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 9 cut(s) 49, 61, 397, 448, 528, 633, 1041, 1241, 1317
MlyI GAGTC 1 cut(s) 1388
MmeI TCCRAC 1 cut(s) 1405
Mph1103I ATGCAT 1 cut(s) 753
MseI TTAA 5 cut(s) 785, 858, 1044, 1167, 1244
MslI CAYNNNNRTG 3 cut(s) 177, 237, 891
MspA1I CMGCKG 2 cut(s) 1034, 1085
MspR9I CCNGG 1 cut(s) 67
MvaI CCWGG 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 1308
NcoI CCATGG 1 cut(s) 892
NdeII GATC 5 cut(s) 69, 253, 316, 709, 907
NheI GCTAGC 2 cut(s) 587, 1298
NlaIV GGNNCC 4 cut(s) 318, 711, 765, 766
NmeAIII GCCGAG 1 cut(s) 901
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 1 cut(s) 1005
PaeR7I CTCGAG 1 cut(s) 774
PagI TCATGA 1 cut(s) 199
PfeI GAWTC 2 cut(s) 644, 1324
PflMI CCANNNNNTGG 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 65
PkrI GCNGC 3 cut(s) 1266, 1297, 1304
PleI GAGTC 1 cut(s) 1388
PpsI GAGTC 1 cut(s) 1388
PpuMI RGGWCCY 2 cut(s) 764, 1162
PshAI GACNNNNGTC 1 cut(s) 1225
Psp5II RGGWCCY 2 cut(s) 764, 1162
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 4 cut(s) 318, 711, 765, 766
PspPI GGNCC 4 cut(s) 764, 992, 1162, 1363
PspPPI RGGWCCY 2 cut(s) 764, 1162
PstNI CAGNNNCTG 2 cut(s) 467, 1308
PsuI RGATCY 2 cut(s) 316, 709
PvuII CAGCTG 2 cut(s) 1034, 1085
RsaI GTAC 1 cut(s) 1008
RsaNI GTAC 1 cut(s) 1007
RseI CAYNNNNRTG 3 cut(s) 177, 237, 891
SaqAI TTAA 5 cut(s) 785, 858, 1044, 1167, 1244
SatI GCNGC 3 cut(s) 1265, 1296, 1303
Sau3AI GATC 5 cut(s) 69, 253, 316, 709, 907
Sau96I GGNCC 4 cut(s) 764, 992, 1162, 1363
SchI GAGTC 1 cut(s) 1388
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 1 cut(s) 60
SfaNI GCATC 2 cut(s) 859, 1282
SfcI CTRYAG 2 cut(s) 1035, 1080
Sfr274I CTCGAG 1 cut(s) 774
SinI GGWCC 3 cut(s) 764, 992, 1162
SlaI CTCGAG 1 cut(s) 774
SmiMI CAYNNNNRTG 3 cut(s) 177, 237, 891
SmlI CTYRAG 4 cut(s) 155, 774, 1010, 1419
SmoI CTYRAG 4 cut(s) 155, 774, 1010, 1419
Sse9I AATT 9 cut(s) 49, 61, 397, 448, 528, 633, 1041, 1241, 1317
SspMI CTAG 7 cut(s) 207, 437, 588, 938, 1277, 1299, 1314
StyD4I CCNGG 1 cut(s) 65
StyI CCWWGG 2 cut(s) 892, 1385
TaaI ACNGT 4 cut(s) 469, 601, 959, 1100
TaqI TCGA 3 cut(s) 385, 775, 1258
TasI AATT 9 cut(s) 49, 61, 397, 448, 528, 633, 1041, 1241, 1317
TatI WGTACW 1 cut(s) 1006
TfiI GAWTC 2 cut(s) 644, 1324
Tru1I TTAA 5 cut(s) 785, 858, 1044, 1167, 1244
Tru9I TTAA 5 cut(s) 785, 858, 1044, 1167, 1244
TscAI CASTG 3 cut(s) 79, 1002, 1236
TseI GCWGC 3 cut(s) 1264, 1295, 1302
TspDTI ATGAA 4 cut(s) 221, 432, 1172, 1242
TspRI CASTG 3 cut(s) 79, 1002, 1236
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 3 cut(s) 764, 992, 1162
XapI RAATTY 3 cut(s) 397, 633, 1317
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 1 cut(s) 1005
XhoI CTCGAG 1 cut(s) 774
XmiI GTMKAC 2 cut(s) 974, 1095
XspI CTAG 7 cut(s) 207, 437, 588, 938, 1277, 1299, 1314
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.