FvH4_2g27990

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000931
Physical Location & Seq
Reverse (-)
22094450 .. 22097619
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27990.t1

Sequence Viewer

Length: 1548 bp
ATGGGGCCGGTTTCTTCGGACGATGAAAAATTGGATGAGAGGGCTAAGAGGCGTTTGAGGAGAAGGTTAAAGGCTAATGGAATTCACAAGCCCGATGTGTGTACTGCGAAGAAGAGCAGAGTGTGTAATGCACAAGTGGAGAGCTTAATGTTGGATGATGACTATGATGAGTACTTTAATAAGATTCAGAAGTATGGGTGGGACTGGATTAAACATTCTAGTGGTCGTGATGTTAATGGCGATTGCGAGAAGGAAGGTAATGATACCAGGGTTGTGGAGGATAGTATTGATAGGAATGAACATTGTGGTGCAGATGATGGTGATGGTGGGAATTCCCGTTTAGGTGATGATGATGACATGGATCCTCAGTATAAGTTATTCTGGGACAATTTGAGGGAAAATGGAAATTCATATGTACTTCAGATAGTTCAAGAGGATGGACGTGTTAAAGAGATAAGGTATCACCAAGACAAGGGCGCACCTGATGAGGTCGATTTAGATAATATGCATTCCCTGGGGAAAGAGAAGACTCCCCAATTTAAAAACAGGGAGATTGTGGAAAATGTAGAGAAGGCCCCAGAGAAAAGGAGTTCAGTGAAGAAGAGGAATGTAAAATTTGAAGCTCCGGAACCTGTTTCAGATGGAATACATGTGCGTTCTAGTAAAAGACATTGTTTAGAGGTTCCAAACACTCCAGACATTTTCGGAAGTTCTCAGCTGAAAGAGAAAACTGGAACCAGAAAAAATTCGAGGATGGTCATTAAGCAGAGAAATTTAGATGATTGTCCACAGAAAAAGAATGGTCTTGAGAAAAAAAGTTCAATGTTGAGGAAGAATGTGAAAGAGACAGACACAGATAATCTTTCTGGTAGAACAAATGGGGGTTCTAATAAAAATTCTGGTTCAGAATATCTTCAGTTCCCAGAGGGTGGGTGCAACAATGAAGCTGAATCCGATGCTAATCTAACAGATGAGAACTATCATGAGTTCTTAAGTTGCAGTAAGAATGATGGTAAAGGTTTGGTCTTTACATCCAGAATCTGCGGACCATTAAGATATGATGAAGAAAGTTCATCGGATTCAGATGAAGAAAGTTCATCGGATTCAGATGTGATTGTGCTTGACAGGGATCCACATGAGGGATACCATACACAAATTACGATGGATGTAGATAGTCGGCGGTCTACTGAAAGTCATTCCCAGTTTAGGCAAGGGATTATGCAGGATCTTATAAGGCCTTACCACCTAGAAGAGTATCTCAGTCTCTTGGAAGACTGGTCCCGCCAAAGGCCACCAACGGGTCATGACAAAAATCTGCGAAATGGAAAAGTAAAATCGTATGCACTGGAAGGGGAATTCGGAAGATCATATCAAGAGATGTACAAAGATCTGGCAAGAAAGATGGATGCCGTGACAGGCGATTATCGCCCTAGAAAGTTGAACCTTTTGCGGGGATTTTTCTACTGGTTGAAGAATATTGCCCAGCATGGAAGCTTTAAACCTTGGAATGACAAAGCATGTTTGAAAGTGTTGCCGCAGAAGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

516

Amino Acids

59.13

Weight (kDa)

5.99

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1232
AccB7I CCANNNNNTGG 1 cut(s) 929
AccI GTMKAC 1 cut(s) 1184
AccIII TCCGGA 1 cut(s) 625
AciI CCGC 5 cut(s) 1044, 1180, 1282, 1450, 1535
AclWI GGATC 5 cut(s) 356, 369, 1124, 1137, 1233
AcsI RAATTY 8 cut(s) 81, 331, 406, 614, 745, 772, 895, 1355
AcuI CTGAAG 2 cut(s) 404, 899
AfaI GTAC 4 cut(s) 103, 173, 417, 1382
AfiI CCNNNNNNNGG 7 cut(s) 472, 929, 1139, 1206, 1287, 1298, 1450
AflII CTTAAG 1 cut(s) 991
AflIII ACRYGT 2 cut(s) 442, 649
AgsI TTSAA 6 cut(s) 431, 620, 822, 1441, 1471, 1525
AjiI CACGTC 1 cut(s) 443
AjnI CCWGG 2 cut(s) 266, 513
AluBI AGCT 5 cut(s) 144, 623, 718, 947, 1494
AluI AGCT 5 cut(s) 144, 623, 718, 947, 1494
Alw26I GTCTC 2 cut(s) 839, 1268
AlwI GGATC 5 cut(s) 356, 369, 1124, 1137, 1233
AlwNI CAGNNNCTG 1 cut(s) 1041
Aor13HI TCCGGA 1 cut(s) 625
AoxI GGCC 4 cut(s) 5, 573, 1235, 1289
ApoI RAATTY 8 cut(s) 81, 331, 406, 614, 745, 772, 895, 1355
Asp700I GAANNNNTTC 2 cut(s) 745, 912
AspLEI GCGC 1 cut(s) 479
AspS9I GGNCC 4 cut(s) 5, 574, 1046, 1278
AsuHPI GGTGA 3 cut(s) 332, 356, 455
AvaII GGWCC 2 cut(s) 1046, 1278
BaeI ACNNNNGTAYC 2 cut(s) 255, 288
BamHI GGATCC 2 cut(s) 361, 1129
BbsI GAAGAC 2 cut(s) 533, 1278
BccI CCATC 8 cut(s) 311, 317, 431, 635, 748, 1004, 1156, 1396
BceAI ACGGC 1 cut(s) 1394
BciT130I CCWGG 2 cut(s) 268, 515
BciVI GTATCC 1 cut(s) 1136
BcoDI GTCTC 2 cut(s) 839, 1268
BfaI CTAG 4 cut(s) 219, 660, 1247, 1431
BfrI CTTAAG 1 cut(s) 991
BfuI GTATCC 1 cut(s) 1136
BglII AGATCT 1 cut(s) 1387
BisI GCNGC 1 cut(s) 1535
BlsI GCNGC 1 cut(s) 1536
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 2 cut(s) 268, 515
Bme18I GGWCC 2 cut(s) 1046, 1278
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 4 cut(s) 5, 574, 1046, 1278
BmiI GGNNCC 8 cut(s) 6, 363, 576, 630, 684, 736, 1131, 1280
BmrFI CCNGG 2 cut(s) 268, 515
BmrI ACTGGG 1 cut(s) 1195
BmsI GCATC 2 cut(s) 946, 1396
BmuI ACTGGG 1 cut(s) 1195
BpiI GAAGAC 2 cut(s) 533, 1278
BpmI CTGGAG 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 827
BsaBI GATNNNNATC 1 cut(s) 960
BsaJI CCNNGG 4 cut(s) 267, 513, 514, 1502
BsaWI WCCGGW 1 cut(s) 625
Bsc4I CCNNNNNNNGG 7 cut(s) 472, 929, 1139, 1206, 1287, 1298, 1450
Bse118I RCCGGY 1 cut(s) 7
Bse1I ACTGG 6 cut(s) 209, 736, 1201, 1280, 1350, 1469
Bse8I GATNNNNATC 1 cut(s) 960
BseAI TCCGGA 1 cut(s) 625
BseBI CCWGG 2 cut(s) 268, 515
BseDI CCNNGG 4 cut(s) 267, 513, 514, 1502
BseGI GGATG 7 cut(s) 40, 160, 442, 759, 1031, 1171, 1411
BseJI GATNNNNATC 1 cut(s) 960
BseLI CCNNNNNNNGG 7 cut(s) 472, 929, 1139, 1206, 1287, 1298, 1450
BseMII CTCAG 3 cut(s) 380, 728, 1273
BseNI ACTGG 6 cut(s) 209, 736, 1201, 1280, 1350, 1469
BseRI GAGGAG 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 1482
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 4 cut(s) 7, 575, 1237, 1291
BsiSI CCGG 2 cut(s) 8, 626
BslFI GGGAC 3 cut(s) 215, 398, 1264
BslI CCNNNNNNNGG 7 cut(s) 472, 929, 1139, 1206, 1287, 1298, 1450
BsmAI GTCTC 2 cut(s) 839, 1268
BsmFI GGGAC 3 cut(s) 215, 398, 1264
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 4 cut(s) 7, 575, 1237, 1291
Bsp13I TCCGGA 1 cut(s) 625
Bsp1407I TGTACA 1 cut(s) 1380
Bsp143I GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
BspACI CCGC 5 cut(s) 1044, 1180, 1282, 1450, 1535
BspANI GGCC 4 cut(s) 7, 575, 1237, 1291
BspCNI CTCAG 3 cut(s) 379, 727, 1272
BspEI TCCGGA 1 cut(s) 625
BspHI TCATGA 2 cut(s) 982, 1303
BspLI GGNNCC 8 cut(s) 6, 363, 576, 630, 684, 736, 1131, 1280
BspPI GGATC 5 cut(s) 356, 369, 1124, 1137, 1233
BspQI GCTCTTC 1 cut(s) 107
BspTI CTTAAG 1 cut(s) 991
BsrFI RCCGGY 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 1380
BsrI ACTGG 6 cut(s) 209, 736, 1201, 1280, 1350, 1469
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 4 cut(s) 267, 513, 514, 1502
BssMI GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
BssT1I CCWWGG 1 cut(s) 1502
Bst2UI CCWGG 2 cut(s) 268, 515
Bst6I CTCTTC 3 cut(s) 107, 596, 1245
BstAFI CTTAAG 1 cut(s) 991
BstAUI TGTACA 1 cut(s) 1380
BstDEI CTNAG 4 cut(s) 45, 366, 714, 1259
BstF5I GGATG 7 cut(s) 40, 160, 442, 759, 1031, 1171, 1411
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 5 cut(s) 364, 1132, 1228, 1367, 1390
BstMAI GTCTC 2 cut(s) 839, 1268
BstMBI GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
BstMWI GCNNNNNNNGC 2 cut(s) 1425, 1540
BstNI CCWGG 2 cut(s) 268, 515
BstNSI RCATGY 2 cut(s) 653, 1521
BstSCI CCNGG 2 cut(s) 266, 513
BstV2I GAAGAC 2 cut(s) 533, 1278
BstX2I RGATCY 4 cut(s) 361, 1129, 1225, 1387
BstXI CCANNNNNNTGG 1 cut(s) 274
BstYI RGATCY 4 cut(s) 361, 1129, 1225, 1387
BsuI GTATCC 1 cut(s) 1136
BsuRI GGCC 4 cut(s) 7, 575, 1237, 1291
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 7 cut(s) 40, 160, 442, 759, 1031, 1171, 1411
BtsIMutI CAGTG 2 cut(s) 600, 1343
CaiI CAGNNNCTG 1 cut(s) 1041
CciI TCATGA 2 cut(s) 982, 1303
CfoI GCGC 1 cut(s) 479
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 4 cut(s) 5, 574, 1046, 1278
Csp6I GTAC 4 cut(s) 102, 172, 416, 1381
CviAII CATG 7 cut(s) 358, 650, 983, 1136, 1304, 1487, 1518
CviQI GTAC 4 cut(s) 102, 172, 416, 1381
DdeI CTNAG 4 cut(s) 45, 366, 714, 1259
DpnI GATC 5 cut(s) 363, 1131, 1227, 1366, 1389
DpnII GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
DraI TTTAAA 2 cut(s) 541, 1498
Eam1104I CTCTTC 3 cut(s) 107, 596, 1245
EarI CTCTTC 3 cut(s) 107, 596, 1245
Eco130I CCWWGG 1 cut(s) 1502
Eco147I AGGCCT 1 cut(s) 1237
Eco47I GGWCC 2 cut(s) 1046, 1278
Eco57I CTGAAG 2 cut(s) 404, 899
EcoO109I RGGNCCY 1 cut(s) 574
EcoRI GAATTC 3 cut(s) 81, 331, 1355
EcoRII CCWGG 2 cut(s) 266, 513
EcoT14I CCWWGG 1 cut(s) 1502
EcoT22I ATGCAT 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 1502
FaeI CATG 7 cut(s) 361, 653, 986, 1139, 1307, 1490, 1521
FaqI GGGAC 3 cut(s) 215, 398, 1264
FatI CATG 7 cut(s) 357, 649, 982, 1135, 1303, 1486, 1517
FauI CCCGC 2 cut(s) 1289, 1443
FauNDI CATATG 1 cut(s) 412
FblI GTMKAC 1 cut(s) 1184
Fnu4HI GCNGC 1 cut(s) 1535
FokI GGATG 7 cut(s) 47, 167, 449, 766, 1018, 1178, 1418
Fsp4HI GCNGC 1 cut(s) 1535
FspBI CTAG 4 cut(s) 219, 660, 1247, 1431
GlaI GCGC 1 cut(s) 478
GluI GCNGC 1 cut(s) 1535
GsaI CCCAGC 1 cut(s) 1486
GsuI CTGGAG 1 cut(s) 678
HaeIII GGCC 4 cut(s) 7, 575, 1237, 1291
HapII CCGG 2 cut(s) 8, 626
HhaI GCGC 1 cut(s) 479
Hin1II CATG 7 cut(s) 361, 653, 986, 1139, 1307, 1490, 1521
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HindIII AAGCTT 1 cut(s) 1492
HinfI GANTC 6 cut(s) 184, 529, 950, 1038, 1079, 1103
HpaII CCGG 2 cut(s) 8, 626
HphI GGTGA 3 cut(s) 332, 356, 455
Hpy166II GTNNAC 3 cut(s) 102, 788, 1185
Hpy188III TCNNGA 9 cut(s) 227, 431, 626, 695, 806, 983, 1035, 1304, 1373
Hpy8I GTNNAC 3 cut(s) 102, 788, 1185
HpyAV CCTTC 5 cut(s) 57, 244, 248, 565, 1343
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 7 cut(s) 131, 311, 508, 936, 999, 1222, 1343
HpyF10VI GCNNNNNNNGC 2 cut(s) 1425, 1540
HpyF3I CTNAG 4 cut(s) 45, 366, 714, 1259
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 7 cut(s) 361, 653, 986, 1139, 1307, 1490, 1521
HspAI GCGC 1 cut(s) 477
Kpn2I TCCGGA 1 cut(s) 625
Kzo9I GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
LguI GCTCTTC 1 cut(s) 107
LmnI GCTCC 1 cut(s) 628
LweI GCATC 2 cut(s) 946, 1396
MaeI CTAG 4 cut(s) 219, 660, 1247, 1431
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 1 cut(s) 1411
MalI GATC 5 cut(s) 363, 1131, 1227, 1366, 1389
MboI GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
MflI RGATCY 4 cut(s) 361, 1129, 1225, 1387
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 1 cut(s) 132
Mph1103I ATGCAT 1 cut(s) 510
MroI TCCGGA 1 cut(s) 625
MroXI GAANNNNTTC 2 cut(s) 745, 912
MslI CAYNNNNRTG 2 cut(s) 219, 306
MspA1I CMGCKG 1 cut(s) 718
MspCI CTTAAG 1 cut(s) 991
MspI CCGG 2 cut(s) 8, 626
MspR9I CCNGG 2 cut(s) 268, 515
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 2 cut(s) 268, 515
MwoI GCNNNNNNNGC 2 cut(s) 1425, 1540
NdeI CATATG 1 cut(s) 412
NdeII GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
NlaIII CATG 7 cut(s) 361, 653, 986, 1139, 1307, 1490, 1521
NlaIV GGNNCC 8 cut(s) 6, 363, 576, 630, 684, 736, 1131, 1280
NmuCI GTSAC 1 cut(s) 1411
NsiI ATGCAT 1 cut(s) 510
NspI RCATGY 2 cut(s) 653, 1521
PagI TCATGA 2 cut(s) 982, 1303
PasI CCCWGGG 1 cut(s) 514
PceI AGGCCT 1 cut(s) 1237
PciI ACATGT 1 cut(s) 649
PciSI GCTCTTC 1 cut(s) 107
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 2 cut(s) 745, 912
PfeI GAWTC 5 cut(s) 184, 950, 1038, 1079, 1103
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 1 cut(s) 1536
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
PscI ACATGT 1 cut(s) 649
PsiI TTATAA 1 cut(s) 1232
Psp6I CCWGG 2 cut(s) 266, 513
PspFI CCCAGC 1 cut(s) 1482
PspGI CCWGG 2 cut(s) 266, 513
PspN4I GGNNCC 8 cut(s) 6, 363, 576, 630, 684, 736, 1131, 1280
PspPI GGNCC 4 cut(s) 5, 574, 1046, 1278
PstNI CAGNNNCTG 1 cut(s) 1041
PsuI RGATCY 4 cut(s) 361, 1129, 1225, 1387
PvuII CAGCTG 1 cut(s) 718
RsaI GTAC 4 cut(s) 103, 173, 417, 1382
RsaNI GTAC 4 cut(s) 102, 172, 416, 1381
RseI CAYNNNNRTG 2 cut(s) 219, 306
SapI GCTCTTC 1 cut(s) 107
SatI GCNGC 1 cut(s) 1535
Sau3AI GATC 5 cut(s) 361, 1129, 1225, 1364, 1387
Sau96I GGNCC 4 cut(s) 5, 574, 1046, 1278
ScaI AGTACT 1 cut(s) 173
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 2 cut(s) 268, 515
SfaNI GCATC 2 cut(s) 946, 1396
SinI GGWCC 2 cut(s) 1046, 1278
SmiMI CAYNNNNRTG 2 cut(s) 219, 306
SmlI CTYRAG 2 cut(s) 806, 991
SmoI CTYRAG 2 cut(s) 806, 991
SseBI AGGCCT 1 cut(s) 1237
SsiI CCGC 5 cut(s) 1044, 1180, 1282, 1450, 1535
SspI AATATT 1 cut(s) 1477
SspMI CTAG 4 cut(s) 219, 660, 1247, 1431
StuI AGGCCT 1 cut(s) 1237
StyD4I CCNGG 2 cut(s) 266, 513
StyI CCWWGG 1 cut(s) 1502
TaiI ACGT 1 cut(s) 445
TaqI TCGA 2 cut(s) 492, 749
TatI WGTACW 4 cut(s) 101, 171, 415, 1380
TauI GCSGC 1 cut(s) 1537
TfiI GAWTC 5 cut(s) 184, 950, 1038, 1079, 1103
TscAI CASTG 2 cut(s) 600, 1350
TseFI GTSAC 1 cut(s) 1411
Tsp45I GTSAC 1 cut(s) 1411
TspDTI ATGAA 8 cut(s) 39, 312, 399, 957, 1062, 1077, 1086, 1101
TspRI CASTG 2 cut(s) 600, 1350
Van91I CCANNNNNTGG 1 cut(s) 929
Vha464I CTTAAG 1 cut(s) 991
VpaK11BI GGWCC 2 cut(s) 1046, 1278
XapI RAATTY 8 cut(s) 81, 331, 406, 614, 745, 772, 895, 1355
XceI RCATGY 2 cut(s) 653, 1521
XmiI GTMKAC 1 cut(s) 1184
XmnI GAANNNNTTC 2 cut(s) 745, 912
XspI CTAG 4 cut(s) 219, 660, 1247, 1431
ZrmI AGTACT 1 cut(s) 173
Zsp2I ATGCAT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.