pycom15g12010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000931
Physical Location & Seq
Forward (+)
8158704 .. 8161231
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12010.1

Sequence Viewer

Length: 1695 bp
ATGGCGCCAACTACATTGATAAGAGAGCTCAACAGTTTAAAGAAGGATTTTGAGGATGGTATGGGTATGGGATTAGTTAGGTGGGGGAAGAGAGAGCGTGTGGCCGATAGGATATTTGAGCCCAATTCCTGCGGCAGTGCCACCAAGAAAAGAAGAAAGTGTAGTACTAAAGTTGAAGAACTGATACCGGATGAGGATTATGAGACATTCTTGAGTTCTTTAGCTGACCATGGCAGTGATGTTGATTATATTGACAATAGATGGGTGGATGATCGGAATGGTGGTGATGTCAATGTAAATGATAGGTCTGTGGATGATGGCGATCACAACGTTGAGGTTAGGTCTTTGGATGATGGTGTTGATGGTGGTGATGATGAGGATCCTGATTATAAGATACTCTTGGATAAATTGACGAAAGGTGGAAAGTCCTTCAGAAACAATAGGTCTGTGGATGATGGTTATGATGATGATGATGATGATGATGATGTCCATGCAAATGGTAGGTATGCGGATGATGGTGGTGAAAACATTCAGTCTCTGGATGATAGTCTTGATGATGAGGATCCTCAGTATAAGATATTCTTGGAAAAGTTGAGGCAAGATGGAAAGTCTTATGTGTTGGAAGCTGTTGGAGATAATGGGAAATCAGAAGTGATCAAGTATGAACAAGAAAATGAGATACTTGACGGGCCCGATTTTGACACCCCTGAAACTTTGAAAAAATCTCAGGTGAAAGAGAAGCCTGATATTCAAAGCACTTTAAGGAGGAGTATGAAGAGAAAATGCACAAATTGCCTTAAAAATTCTGCTCTTCCTGTGAATAAAGAAGTGATTGCGAGCCCAAGAATTGTTAAGTATGATCTGAGGAAAGAAAACAGGGCAATTCTGGAAAACAGCTCGATGAAGAAGAAGAAGAAGAATGTAAATGAGAAAGCTGCAAATGGTAGCACACGGGGGCGTCCTTTGAAAATACCCCCTGTGGAGGCTGTTCATTTTACCATGCAAGGATGTGCAAATGAGAAAGCTGCTAAAGGTAGCACAAAGGAGTGCCCTGTTAAAAGAACTCGTGTGGCGGATCTCCGGTTTGCCAAGCAAGGATGCAAGCGTGGATTAGAAGATGAGGACTACACAAAGCTTTTAAGTTGTCTACAGTTTGATGATGGTAAAATGGTGTATTCACCTCCAAATGGTAGACCGGTGATACTGGATGATGATGGAGAGAGTTCACCTCCAAATAGAAGTTTTACTTCATTTACAGCTGTGGATGTAGACAGTGGGCGGTGCGTTAAAACTCATGATGCAAGTCATTCGCAGTTTAGGAAGGGGCTTATGGATATTCTGACCAGGCCTTATGACTATGAAGAGTACAGAAGCCTCTTGAAAGAAGCGACTCACCGAAAGCCATTGGGGCACGACAGAAATCTGCGCAATAGAACAATAACCTGTCCAGTTGAGGGGAAACTCGCACCTTCATATCTAAAACAGCACATAGTTCTGAGAAGAAAGATTACGGCAGCTCGTGGTGACCGCCCTAGAGTTTTGAATCTCTTGCGGGGATTTTTCTACTGGCTGAAGAATGTGGCCCAAGATGAAAGCTTCTGTCCTTGGAACGACTCGTCTTGTTTGAAAGTGCTGCCACCATCTGATAAGAAGGCTGGACTTACAGAAAGGGGACTTGAAGGCAAACGTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

565

Amino Acids

63.78

Weight (kDa)

6.24

Isoelectric Point (pI)

47.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 390
Acc16I TGCGCA 1 cut(s) 1427
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 3 cut(s) 1147, 1192, 1269
AciI CCGC 6 cut(s) 132, 509, 1073, 1279, 1528, 1552
AclI AACGTT 1 cut(s) 330
AclWI GGATC 5 cut(s) 374, 387, 557, 570, 1083
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 2 cut(s) 415, 1592
AcyI GRCGYC 2 cut(s) 5, 958
AfaI GTAC 2 cut(s) 166, 1367
AfiI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
AgeI ACCGGT 1 cut(s) 1195
AgsI TTSAA 8 cut(s) 176, 718, 752, 967, 1381, 1543, 1627, 1679
AjnI CCWGG 1 cut(s) 1343
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 2 cut(s) 197, 540
AlwI GGATC 5 cut(s) 374, 387, 557, 570, 1083
AlwNI CAGNNNCTG 1 cut(s) 538
AoxI GGCC 4 cut(s) 102, 689, 1346, 1581
ApaI GGGCCC 1 cut(s) 693
ApeKI GCWGC 4 cut(s) 935, 1025, 1514, 1633
ApoI RAATTY 1 cut(s) 802
AsiGI ACCGGT 1 cut(s) 1195
Asp700I GAANNNNTTC 1 cut(s) 528
AspLEI GCGC 2 cut(s) 7, 1428
AspS9I GGNCC 3 cut(s) 689, 690, 1582
AsuHPI GGTGA 9 cut(s) 296, 380, 533, 742, 1170, 1210, 1218, 1385, 1535
BaeGI GKGCMC 3 cut(s) 693, 1052, 1413
BamHI GGATCC 2 cut(s) 379, 562
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 4 cut(s) 30, 123, 693, 842
BarI GAAGNNNNNNTAC 4 cut(s) 168, 200, 1492, 1524
BauI CACGAG 2 cut(s) 1065, 1518
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 4 cut(s) 922, 1012, 1526, 1620
BceAI ACGGC 1 cut(s) 1527
BcgI CGANNNNNNTGC 2 cut(s) 1290, 1324
BciT130I CCWGG 1 cut(s) 1345
BclI TGATCA 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 197, 540
BfaI CTAG 1 cut(s) 1533
BfmI CTRYAG 1 cut(s) 1148
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 1408
BisI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
BlsI GCNGC 5 cut(s) 134, 937, 1027, 1516, 1635
BmcAI AGTACT 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 1345
BmgT120I GGNCC 3 cut(s) 689, 690, 1582
BmiI GGNNCC 4 cut(s) 6, 381, 564, 691
BmrFI CCNGG 1 cut(s) 1345
BmsI GCATC 2 cut(s) 1088, 1288
BplI GAGNNNNNCTC 2 cut(s) 1213, 1245
BpuEI CTTGAG 1 cut(s) 232
BsaBI GATNNNNATC 3 cut(s) 321, 378, 561
BsaHI GRCGYC 2 cut(s) 5, 958
BsaJI CCNNGG 2 cut(s) 229, 1604
BsaWI WCCGGW 3 cut(s) 187, 1080, 1195
Bsc4I CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
Bse118I RCCGGY 1 cut(s) 1195
Bse1I ACTGG 3 cut(s) 1209, 1448, 1571
Bse8I GATNNNNATC 3 cut(s) 321, 378, 561
BseBI CCWGG 1 cut(s) 1345
BseDI CCNNGG 2 cut(s) 229, 1604
BseJI GATNNNNATC 3 cut(s) 321, 378, 561
BseLI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
BseMII CTCAG 4 cut(s) 581, 740, 854, 1487
BseNI ACTGG 3 cut(s) 1209, 1448, 1571
BseRI GAGGAG 1 cut(s) 781
BseSI GKGCMC 3 cut(s) 693, 1052, 1413
BseXI GCAGC 4 cut(s) 922, 1012, 1526, 1620
BshFI GGCC 4 cut(s) 104, 691, 1348, 1583
BshNI GGYRCC 1 cut(s) 4
BshTI ACCGGT 1 cut(s) 1195
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 3 cut(s) 188, 1081, 1196
BslFI GGGAC 1 cut(s) 1686
BslI CCNNNNNNNGG 3 cut(s) 982, 1187, 1454
BsmAI GTCTC 2 cut(s) 197, 540
BsmFI GGGAC 1 cut(s) 1686
BsnI GGCC 4 cut(s) 104, 691, 1348, 1583
Bsp120I GGGCCC 1 cut(s) 689
Bsp1286I GDGCHC 6 cut(s) 30, 123, 693, 842, 1052, 1413
Bsp143I GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 6 cut(s) 132, 509, 1073, 1279, 1528, 1552
BspANI GGCC 4 cut(s) 104, 691, 1348, 1583
BspCNI CTCAG 4 cut(s) 580, 739, 855, 1488
BspHI TCATGA 1 cut(s) 1294
BspLI GGNNCC 4 cut(s) 6, 381, 564, 691
BspPI GGATC 5 cut(s) 374, 387, 557, 570, 1083
BspQI GCTCTTC 1 cut(s) 816
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 1195
BsrI ACTGG 3 cut(s) 1209, 1448, 1571
BssAI RCCGGY 1 cut(s) 1195
BssECI CCNNGG 2 cut(s) 229, 1604
BssMI GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
BssNI GRCGYC 2 cut(s) 5, 958
BssSI CACGAG 2 cut(s) 1065, 1518
BssT1I CCWWGG 2 cut(s) 229, 1604
Bst2BI CACGAG 2 cut(s) 1065, 1518
Bst2UI CCWGG 1 cut(s) 1345
Bst4CI ACNGT 3 cut(s) 35, 1152, 1274
Bst6I CTCTTC 4 cut(s) 83, 770, 816, 1356
BstACI GRCGYC 2 cut(s) 5, 958
BstAPI GCANNNNNTGC 1 cut(s) 792
BstC8I GCNNGC 2 cut(s) 838, 1103
BstDEI CTNAG 4 cut(s) 567, 726, 863, 1496
BstDSI CCRYGG 1 cut(s) 229
BstEII GGTNACC 1 cut(s) 1523
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 1428
BstKTI GATC 7 cut(s) 274, 325, 382, 565, 657, 862, 1078
BstMAI GTCTC 2 cut(s) 197, 540
BstMBI GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
BstMWI GCNNNNNNNGC 2 cut(s) 792, 1408
BstNI CCWGG 1 cut(s) 1345
BstPI GGTNACC 1 cut(s) 1523
BstSCI CCNGG 1 cut(s) 1343
BstSFI CTRYAG 1 cut(s) 1148
BstSLI GKGCMC 3 cut(s) 693, 1052, 1413
BstV1I GCAGC 4 cut(s) 922, 1012, 1526, 1620
BstX2I RGATCY 3 cut(s) 379, 562, 1075
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 3 cut(s) 379, 562, 1075
BsuRI GGCC 4 cut(s) 104, 691, 1348, 1583
BtgI CCRYGG 1 cut(s) 229
BtsI GCAGTG 2 cut(s) 142, 241
BtsIMutI CAGTG 3 cut(s) 142, 241, 1279
Cac8I GCNNGC 2 cut(s) 838, 1103
CaiI CAGNNNCTG 1 cut(s) 538
CciI TCATGA 1 cut(s) 1294
CfoI GCGC 2 cut(s) 7, 1428
Cfr10I RCCGGY 1 cut(s) 1195
Cfr13I GGNCC 3 cut(s) 689, 690, 1582
CseI GACGC 1 cut(s) 947
Csp6I GTAC 2 cut(s) 165, 1366
CspAI ACCGGT 1 cut(s) 1195
CviAII CATG 4 cut(s) 230, 491, 1000, 1295
CviQI GTAC 2 cut(s) 165, 1366
DdeI CTNAG 4 cut(s) 567, 726, 863, 1496
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 273, 324, 381, 564, 656, 861, 1077
DpnII GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
DraI TTTAAA 1 cut(s) 39
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 4 cut(s) 83, 770, 816, 1356
EarI CTCTTC 4 cut(s) 83, 770, 816, 1356
EciI GGCGGA 1 cut(s) 1088
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 229, 1604
Eco147I AGGCCT 1 cut(s) 1348
Eco24I GRGCYC 4 cut(s) 30, 123, 693, 842
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 2 cut(s) 415, 1592
Eco91I GGTNACC 1 cut(s) 1523
EcoICRI GAGCTC 1 cut(s) 28
EcoO65I GGTNACC 1 cut(s) 1523
EcoRII CCWGG 1 cut(s) 1343
EcoT14I CCWWGG 2 cut(s) 229, 1604
EcoT38I GRGCYC 4 cut(s) 30, 123, 693, 842
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 229, 1604
FaeI CATG 4 cut(s) 233, 494, 1003, 1298
FalI AAGNNNNNCTT 6 cut(s) 383, 415, 566, 598, 1231, 1263
FaqI GGGAC 1 cut(s) 1686
FatI CATG 4 cut(s) 229, 490, 999, 1294
FauI CCCGC 1 cut(s) 1545
FbaI TGATCA 1 cut(s) 654
FblI GTMKAC 3 cut(s) 1147, 1192, 1269
Fnu4HI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
FriOI GRGCYC 4 cut(s) 30, 123, 693, 842
Fsp4HI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
FspBI CTAG 1 cut(s) 1533
FspI TGCGCA 1 cut(s) 1427
GlaI GCGC 2 cut(s) 6, 1427
GluI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 104, 691, 1348, 1583
HapII CCGG 3 cut(s) 188, 1081, 1196
HgaI GACGC 1 cut(s) 947
HhaI GCGC 2 cut(s) 7, 1428
Hin1I GRCGYC 2 cut(s) 5, 958
Hin1II CATG 4 cut(s) 233, 494, 1003, 1298
Hin6I GCGC 2 cut(s) 5, 1426
HinP1I GCGC 2 cut(s) 5, 1426
HindIII AAGCTT 2 cut(s) 1133, 1594
HinfI GANTC 3 cut(s) 1390, 1543, 1613
HpaII CCGG 3 cut(s) 188, 1081, 1196
HphI GGTGA 9 cut(s) 296, 380, 533, 742, 1170, 1210, 1218, 1385, 1535
Hpy166II GTNNAC 4 cut(s) 1148, 1193, 1226, 1270
Hpy188I TCNGA 7 cut(s) 276, 434, 649, 864, 1341, 1497, 1645
Hpy188III TCNNGA 7 cut(s) 211, 383, 539, 551, 887, 1295, 1378
Hpy8I GTNNAC 4 cut(s) 1148, 1193, 1226, 1270
HpyAV CCTTC 6 cut(s) 37, 439, 1315, 1479, 1645, 1673
HpyCH4III ACNGT 3 cut(s) 35, 1152, 1274
HpyCH4IV ACGT 2 cut(s) 330, 1687
HpyCH4V TGCA 7 cut(s) 494, 786, 938, 1003, 1013, 1101, 1301
HpyF10VI GCNNNNNNNGC 2 cut(s) 792, 1408
HpyF3I CTNAG 4 cut(s) 567, 726, 863, 1496
HpySE526I ACGT 2 cut(s) 330, 1687
Hsp92I GRCGYC 2 cut(s) 5, 958
Hsp92II CATG 4 cut(s) 233, 494, 1003, 1298
HspAI GCGC 2 cut(s) 5, 1426
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
LguI GCTCTTC 1 cut(s) 816
Lsp1109I GCAGC 4 cut(s) 922, 1012, 1526, 1620
LweI GCATC 2 cut(s) 1088, 1288
MaeI CTAG 1 cut(s) 1533
MaeII ACGT 2 cut(s) 330, 1687
MaeIII GTNAC 1 cut(s) 1523
MalI GATC 7 cut(s) 273, 324, 381, 564, 656, 861, 1077
MboI GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
MflI RGATCY 3 cut(s) 379, 562, 1075
MhlI GDGCHC 6 cut(s) 30, 123, 693, 842, 1052, 1413
MluCI AATT 6 cut(s) 124, 407, 790, 802, 846, 882
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 1384, 1607
MmeI TCCRAC 2 cut(s) 600, 610
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 7 cut(s) 38, 761, 798, 852, 1056, 1139, 1287
MslI CAYNNNNRTG 2 cut(s) 234, 495
MspA1I CMGCKG 1 cut(s) 1259
MspI CCGG 3 cut(s) 188, 1081, 1196
MspR9I CCNGG 1 cut(s) 1345
MvaI CCWGG 1 cut(s) 1345
MwoI GCNNNNNNNGC 2 cut(s) 792, 1408
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 229
NdeII GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
NlaIII CATG 4 cut(s) 233, 494, 1003, 1298
NlaIV GGNNCC 4 cut(s) 6, 381, 564, 691
NmuCI GTSAC 1 cut(s) 1523
NsbI TGCGCA 1 cut(s) 1427
PagI TCATGA 1 cut(s) 1294
PceI AGGCCT 1 cut(s) 1348
PciSI GCTCTTC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 1 cut(s) 1543
PinAI ACCGGT 1 cut(s) 1195
PkrI GCNGC 5 cut(s) 134, 937, 1027, 1516, 1635
PleI GAGTC 2 cut(s) 1384, 1607
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 1384, 1607
PsiI TTATAA 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 30
Psp1406I AACGTT 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 1343
PspEI GGTNACC 1 cut(s) 1523
PspGI CCWGG 1 cut(s) 1343
PspN4I GGNNCC 4 cut(s) 6, 381, 564, 691
PspOMI GGGCCC 1 cut(s) 689
PspPI GGNCC 3 cut(s) 689, 690, 1582
PstNI CAGNNNCTG 1 cut(s) 538
PsuI RGATCY 3 cut(s) 379, 562, 1075
PvuII CAGCTG 1 cut(s) 1259
RsaI GTAC 2 cut(s) 166, 1367
RsaNI GTAC 2 cut(s) 165, 1366
RseI CAYNNNNRTG 2 cut(s) 234, 495
SacI GAGCTC 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 816
SaqAI TTAA 7 cut(s) 38, 761, 798, 852, 1056, 1139, 1287
SatI GCNGC 5 cut(s) 133, 936, 1026, 1515, 1634
Sau3AI GATC 7 cut(s) 271, 322, 379, 562, 654, 859, 1075
Sau96I GGNCC 3 cut(s) 689, 690, 1582
ScaI AGTACT 1 cut(s) 166
SchI GAGTC 2 cut(s) 1384, 1607
ScrFI CCNGG 1 cut(s) 1345
SduI GDGCHC 6 cut(s) 30, 123, 693, 842, 1052, 1413
SfaNI GCATC 2 cut(s) 1088, 1288
SfcI CTRYAG 1 cut(s) 1148
SfoI GGCGCC 1 cut(s) 6
SmiMI CAYNNNNRTG 2 cut(s) 234, 495
SmlI CTYRAG 1 cut(s) 211
SmoI CTYRAG 1 cut(s) 211
Sse9I AATT 6 cut(s) 124, 407, 790, 802, 846, 882
SseBI AGGCCT 1 cut(s) 1348
SsiI CCGC 6 cut(s) 132, 509, 1073, 1279, 1528, 1552
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 1533
SstI GAGCTC 1 cut(s) 30
StuI AGGCCT 1 cut(s) 1348
StyD4I CCNGG 1 cut(s) 1343
StyI CCWWGG 2 cut(s) 229, 1604
TaaI ACNGT 3 cut(s) 35, 1152, 1274
TaiI ACGT 2 cut(s) 333, 1690
TaqI TCGA 1 cut(s) 899
TasI AATT 6 cut(s) 124, 407, 790, 802, 846, 882
TatI WGTACW 2 cut(s) 164, 1365
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 1543
Tru1I TTAA 7 cut(s) 38, 761, 798, 852, 1056, 1139, 1287
Tru9I TTAA 7 cut(s) 38, 761, 798, 852, 1056, 1139, 1287
TscAI CASTG 3 cut(s) 142, 241, 1279
TseFI GTSAC 1 cut(s) 1523
TseI GCWGC 4 cut(s) 935, 1025, 1514, 1633
Tsp45I GTSAC 1 cut(s) 1523
TspDTI ATGAA 8 cut(s) 678, 788, 917, 980, 1239, 1374, 1461, 1605
TspRI CASTG 3 cut(s) 142, 241, 1279
XapI RAATTY 1 cut(s) 802
XmiI GTMKAC 3 cut(s) 1147, 1192, 1269
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 1 cut(s) 1533
ZrmI AGTACT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.