No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 846, 2222 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2051 |
| AccI | GTMKAC | 1 cut(s) | 2056 |
| AciI | CCGC | 8 cut(s) | 205, 367, 529, 691, 1945, 2096, 2325, 2443 |
| AcsI | RAATTY | 8 cut(s) | 430, 610, 1054, 1186, 1478, 1527, 2204, 2314 |
| AfaI | GTAC | 3 cut(s) | 2127, 2158, 2384 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 739, 1313, 1572, 2051, 2300 |
| AflIII | ACRYGT | 2 cut(s) | 2036, 2150 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 157 |
| AjnI | CCWGG | 3 cut(s) | 1885, 2422, 2475 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 308, 340, 1274, 1306 |
| AleI | CACNNNNGTG | 2 cut(s) | 804, 921 |
| AluBI | AGCT | 9 cut(s) | 663, 1298, 1387, 1608, 1838, 1989, 2257, 2266, 2487 |
| AluI | AGCT | 9 cut(s) | 663, 1298, 1387, 1608, 1838, 1989, 2257, 2266, 2487 |
| Alw21I | GWGCWC | 2 cut(s) | 1389, 2044 |
| Alw26I | GTCTC | 3 cut(s) | 1721, 1955, 2057 |
| Alw44I | GTGCAC | 1 cut(s) | 2040 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1172, 2533 |
| Ama87I | CYCGRG | 1 cut(s) | 2129 |
| AoxI | GGCC | 3 cut(s) | 1574, 2291, 2472 |
| ApaLI | GTGCAC | 1 cut(s) | 2040 |
| ApeKI | GCWGC | 3 cut(s) | 1166, 1295, 2527 |
| ApoI | RAATTY | 8 cut(s) | 430, 610, 1054, 1186, 1478, 1527, 2204, 2314 |
| AsiSI | GCGATCGC | 1 cut(s) | 2419 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 306, 468, 630, 1264 |
| AspA2I | CCTAGG | 1 cut(s) | 200 |
| AspS9I | GGNCC | 4 cut(s) | 6, 151, 1736, 2473 |
| AvaI | CYCGRG | 1 cut(s) | 2129 |
| AvaII | GGWCC | 3 cut(s) | 6, 151, 1736 |
| AvrII | CCTAGG | 1 cut(s) | 200 |
| AxyI | CCTNAGG | 1 cut(s) | 1422 |
| BaeGI | GKGCMC | 1 cut(s) | 2044 |
| BamHI | GGATCC | 4 cut(s) | 241, 403, 565, 2137 |
| BanII | GRGCYC | 1 cut(s) | 1389 |
| BbsI | GAAGAC | 2 cut(s) | 128, 2280 |
| Bbv12I | GWGCWC | 2 cut(s) | 1389, 2044 |
| BbvI | GCAGC | 2 cut(s) | 1178, 1307 |
| BccI | CCATC | 5 cut(s) | 185, 275, 599, 890, 950 |
| BceAI | ACGGC | 1 cut(s) | 1606 |
| BciT130I | CCWGG | 3 cut(s) | 1887, 2424, 2477 |
| BclI | TGATCA | 1 cut(s) | 2403 |
| BcoDI | GTCTC | 3 cut(s) | 1721, 1955, 2057 |
| BfaI | CTAG | 8 cut(s) | 201, 228, 921, 981, 1158, 1301, 1545, 1809 |
| BfmI | CTRYAG | 3 cut(s) | 888, 1772, 1855 |
| BglII | AGATCT | 1 cut(s) | 2389 |
| BisI | GCNGC | 3 cut(s) | 1167, 1296, 2528 |
| BlnI | CCTAGG | 1 cut(s) | 200 |
| BlsI | GCNGC | 3 cut(s) | 1168, 1297, 2529 |
| Bme1390I | CCNGG | 3 cut(s) | 1887, 2424, 2477 |
| Bme18I | GGWCC | 3 cut(s) | 6, 151, 1736 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 157 |
| BmeT110I | CYCGRG | 1 cut(s) | 2129 |
| BmgT120I | GGNCC | 4 cut(s) | 6, 151, 1736, 2473 |
| BmiI | GGNNCC | 9 cut(s) | 7, 153, 243, 405, 567, 1029, 1368, 1737, 2139 |
| BmrFI | CCNGG | 3 cut(s) | 1887, 2424, 2477 |
| BmrI | ACTGGG | 1 cut(s) | 1113 |
| BmsI | GCATC | 4 cut(s) | 209, 371, 533, 1135 |
| BmuI | ACTGGG | 1 cut(s) | 1113 |
| BpiI | GAAGAC | 2 cut(s) | 128, 2280 |
| BpmI | CTGGAG | 6 cut(s) | 35, 1382, 1386, 1584, 2299, 2396 |
| BpuEI | CTTGAG | 4 cut(s) | 292, 784, 2033, 2246 |
| BsaBI | GATNNNNATC | 3 cut(s) | 240, 402, 564 |
| BsaI | GGTCTC | 1 cut(s) | 1721 |
| BsaJI | CCNNGG | 7 cut(s) | 155, 200, 1008, 1731, 2422, 2475, 2495 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 739, 1313, 1572, 2051, 2300 |
| Bse1I | ACTGG | 7 cut(s) | 627, 1108, 1344, 1566, 2141, 2282, 2462 |
| Bse21I | CCTNAGG | 1 cut(s) | 1422 |
| Bse3DI | GCAATG | 3 cut(s) | 790, 1930, 1954 |
| Bse8I | GATNNNNATC | 3 cut(s) | 240, 402, 564 |
| BseBI | CCWGG | 3 cut(s) | 1887, 2424, 2477 |
| BseDI | CCNNGG | 7 cut(s) | 155, 200, 1008, 1731, 2422, 2475, 2495 |
| BseGI | GGATG | 4 cut(s) | 49, 799, 841, 1072 |
| BseJI | GATNNNNATC | 3 cut(s) | 240, 402, 564 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 739, 1313, 1572, 2051, 2300 |
| BseMI | GCAATG | 3 cut(s) | 790, 1930, 1954 |
| BseMII | CTCAG | 3 cut(s) | 260, 422, 1436 |
| BseNI | ACTGG | 7 cut(s) | 627, 1108, 1344, 1566, 2141, 2282, 2462 |
| BseSI | GKGCMC | 1 cut(s) | 2044 |
| BseXI | GCAGC | 2 cut(s) | 1178, 1307 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2419 |
| BshFI | GGCC | 3 cut(s) | 1576, 2293, 2474 |
| BsiEI | CGRYCG | 1 cut(s) | 2419 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1389, 2044 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2129 |
| BslFI | GGGAC | 2 cut(s) | 922, 1749 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 739, 1313, 1572, 2051, 2300 |
| BsmAI | GTCTC | 3 cut(s) | 1721, 1955, 2057 |
| BsmFI | GGGAC | 2 cut(s) | 922, 1749 |
| BsmI | GAATGC | 4 cut(s) | 1084, 1288, 1561, 2098 |
| BsnI | GGCC | 3 cut(s) | 1576, 2293, 2474 |
| Bso31I | GGTCTC | 1 cut(s) | 1721 |
| BsoBI | CYCGRG | 1 cut(s) | 2129 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1389, 2044 |
| Bsp1407I | TGTACA | 1 cut(s) | 2382 |
| BspACI | CCGC | 8 cut(s) | 205, 367, 529, 691, 1945, 2096, 2325, 2443 |
| BspANI | GGCC | 3 cut(s) | 1576, 2293, 2474 |
| BspCNI | CTCAG | 3 cut(s) | 259, 421, 1435 |
| BspHI | TCATGA | 4 cut(s) | 100, 139, 372, 534 |
| BspLI | GGNNCC | 9 cut(s) | 7, 153, 243, 405, 567, 1029, 1368, 1737, 2139 |
| BspMAI | CTGCAG | 1 cut(s) | 1776 |
| BspQI | GCTCTTC | 1 cut(s) | 1377 |
| BspTNI | GGTCTC | 1 cut(s) | 1721 |
| BsrDI | GCAATG | 3 cut(s) | 790, 1930, 1954 |
| BsrGI | TGTACA | 1 cut(s) | 2382 |
| BsrI | ACTGG | 7 cut(s) | 627, 1108, 1344, 1566, 2141, 2282, 2462 |
| BssECI | CCNNGG | 7 cut(s) | 155, 200, 1008, 1731, 2422, 2475, 2495 |
| BssT1I | CCWWGG | 4 cut(s) | 200, 1008, 1731, 2495 |
| Bst2UI | CCWGG | 3 cut(s) | 1887, 2424, 2477 |
| Bst4CI | ACNGT | 2 cut(s) | 978, 2535 |
| Bst6I | CTCTTC | 3 cut(s) | 692, 1377, 1719 |
| BstAUI | TGTACA | 1 cut(s) | 2382 |
| BstC8I | GCNNGC | 2 cut(s) | 2295, 2413 |
| BstDEI | CTNAG | 7 cut(s) | 162, 246, 408, 1422, 1695, 2003, 2269 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2298 |
| BstF5I | GGATG | 4 cut(s) | 49, 799, 841, 1072 |
| BstMAI | GTCTC | 3 cut(s) | 1721, 1955, 2057 |
| BstMCI | CGRYCG | 1 cut(s) | 2419 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1292, 2263 |
| BstNI | CCWGG | 3 cut(s) | 1887, 2424, 2477 |
| BstNSI | RCATGY | 2 cut(s) | 2040, 2154 |
| BstSCI | CCNGG | 3 cut(s) | 1885, 2422, 2475 |
| BstSFI | CTRYAG | 3 cut(s) | 888, 1772, 1855 |
| BstSLI | GKGCMC | 1 cut(s) | 2044 |
| BstV1I | GCAGC | 2 cut(s) | 1178, 1307 |
| BstV2I | GAAGAC | 2 cut(s) | 128, 2280 |
| BstX2I | RGATCY | 9 cut(s) | 241, 403, 565, 1246, 1303, 1786, 2137, 2227, 2389 |
| BstXI | CCANNNNNNTGG | 4 cut(s) | 353, 515, 677, 1139 |
| BstYI | RGATCY | 9 cut(s) | 241, 403, 565, 1246, 1303, 1786, 2137, 2227, 2389 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1422 |
| BsuRI | GGCC | 3 cut(s) | 1576, 2293, 2474 |
| BtsCI | GGATG | 4 cut(s) | 49, 799, 841, 1072 |
| BtsIMutI | CAGTG | 5 cut(s) | 620, 1170, 1275, 1506, 2345 |
| Cac8I | GCNNGC | 2 cut(s) | 2295, 2413 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1172, 2533 |
| CciI | TCATGA | 4 cut(s) | 100, 139, 372, 534 |
| Cfr13I | GGNCC | 4 cut(s) | 6, 151, 1736, 2473 |
| CsiI | ACCWGGT | 1 cut(s) | 1885 |
| Csp6I | GTAC | 3 cut(s) | 2126, 2157, 2383 |
| CviQI | GTAC | 3 cut(s) | 2126, 2157, 2383 |
| DdeI | CTNAG | 7 cut(s) | 162, 246, 408, 1422, 1695, 2003, 2269 |
| DraI | TTTAAA | 2 cut(s) | 1242, 1716 |
| DriI | GACNNNNNGTC | 1 cut(s) | 157 |
| Eam1104I | CTCTTC | 3 cut(s) | 692, 1377, 1719 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 157 |
| EarI | CTCTTC | 3 cut(s) | 692, 1377, 1719 |
| EciI | GGCGGA | 3 cut(s) | 220, 382, 544 |
| Ecl136II | GAGCTC | 1 cut(s) | 1387 |
| Eco130I | CCWWGG | 4 cut(s) | 200, 1008, 1731, 2495 |
| Eco147I | AGGCCT | 2 cut(s) | 1576, 2293 |
| Eco24I | GRGCYC | 1 cut(s) | 1389 |
| Eco31I | GGTCTC | 1 cut(s) | 1721 |
| Eco32I | GATATC | 1 cut(s) | 823 |
| Eco47I | GGWCC | 3 cut(s) | 6, 151, 1736 |
| Eco53kI | GAGCTC | 1 cut(s) | 1387 |
| Eco81I | CCTNAGG | 1 cut(s) | 1422 |
| Eco88I | CYCGRG | 1 cut(s) | 2129 |
| EcoICRI | GAGCTC | 1 cut(s) | 1387 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2298 |
| EcoRI | GAATTC | 1 cut(s) | 1186 |
| EcoRII | CCWGG | 3 cut(s) | 1885, 2422, 2475 |
| EcoRV | GATATC | 1 cut(s) | 823 |
| EcoT14I | CCWWGG | 4 cut(s) | 200, 1008, 1731, 2495 |
| EcoT22I | ATGCAT | 5 cut(s) | 224, 298, 386, 460, 548 |
| EcoT38I | GRGCYC | 1 cut(s) | 1389 |
| ErhI | CCWWGG | 4 cut(s) | 200, 1008, 1731, 2495 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 155, 187, 245, 277, 407, 439 |
| FaqI | GGGAC | 2 cut(s) | 922, 1749 |
| FauI | CCCGC | 2 cut(s) | 684, 2436 |
| FauNDI | CATATG | 5 cut(s) | 292, 454, 616, 2068, 2341 |
| FbaI | TGATCA | 1 cut(s) | 2403 |
| FblI | GTMKAC | 1 cut(s) | 2056 |
| Fnu4HI | GCNGC | 3 cut(s) | 1167, 1296, 2528 |
| FokI | GGATG | 4 cut(s) | 56, 806, 848, 1079 |
| FriOI | GRGCYC | 1 cut(s) | 1389 |
| Fsp4HI | GCNGC | 3 cut(s) | 1167, 1296, 2528 |
| FspBI | CTAG | 8 cut(s) | 201, 228, 921, 981, 1158, 1301, 1545, 1809 |
| GluI | GCNGC | 3 cut(s) | 1167, 1296, 2528 |
| GsuI | CTGGAG | 6 cut(s) | 35, 1382, 1386, 1584, 2299, 2396 |
| HaeIII | GGCC | 3 cut(s) | 1576, 2293, 2474 |
| HindIII | AAGCTT | 2 cut(s) | 1606, 2485 |
| HinfI | GANTC | 9 cut(s) | 47, 1207, 1534, 1766, 1967, 2111, 2174, 2434, 2448 |
| Hpy166II | GTNNAC | 7 cut(s) | 9, 151, 1227, 2042, 2057, 2077, 2383 |
| Hpy8I | GTNNAC | 7 cut(s) | 9, 151, 1227, 2042, 2057, 2077, 2383 |
| HpyAV | CCTTC | 4 cut(s) | 434, 1822, 2164, 2474 |
| HpyCH4III | ACNGT | 2 cut(s) | 978, 2535 |
| HpyCH4IV | ACGT | 1 cut(s) | 2236 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1292, 2263 |
| HpyF3I | CTNAG | 7 cut(s) | 162, 246, 408, 1422, 1695, 2003, 2269 |
| HpySE526I | ACGT | 1 cut(s) | 2236 |
| Ksp22I | TGATCA | 1 cut(s) | 2403 |
| LguI | GCTCTTC | 1 cut(s) | 1377 |
| Lsp1109I | GCAGC | 2 cut(s) | 1178, 1307 |
| LweI | GCATC | 4 cut(s) | 209, 371, 533, 1135 |
| MabI | ACCWGGT | 1 cut(s) | 1885 |
| MaeI | CTAG | 8 cut(s) | 201, 228, 921, 981, 1158, 1301, 1545, 1809 |
| MaeII | ACGT | 1 cut(s) | 2236 |
| MaeIII | GTNAC | 1 cut(s) | 1940 |
| MflI | RGATCY | 9 cut(s) | 241, 403, 565, 1246, 1303, 1786, 2137, 2227, 2389 |
| MhlI | GDGCHC | 2 cut(s) | 1389, 2044 |
| MlyI | GAGTC | 1 cut(s) | 1201 |
| MmeI | TCCRAC | 1 cut(s) | 705 |
| Mph1103I | ATGCAT | 5 cut(s) | 224, 298, 386, 460, 548 |
| MroXI | GAANNNNTTC | 4 cut(s) | 306, 468, 630, 1264 |
| MseI | TTAA | 9 cut(s) | 774, 942, 1241, 1715, 1748, 2120, 2166, 2208, 2232 |
| MslI | CAYNNNNRTG | 7 cut(s) | 99, 105, 144, 783, 804, 921, 957 |
| MspR9I | CCNGG | 3 cut(s) | 1887, 2424, 2477 |
| Mva1269I | GAATGC | 4 cut(s) | 1084, 1288, 1561, 2098 |
| MvaI | CCWGG | 3 cut(s) | 1887, 2424, 2477 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1292, 2263 |
| NdeI | CATATG | 5 cut(s) | 292, 454, 616, 2068, 2341 |
| NlaIV | GGNNCC | 9 cut(s) | 7, 153, 243, 405, 567, 1029, 1368, 1737, 2139 |
| NmuCI | GTSAC | 1 cut(s) | 1940 |
| NsiI | ATGCAT | 5 cut(s) | 224, 298, 386, 460, 548 |
| NspI | RCATGY | 2 cut(s) | 2040, 2154 |
| OliI | CACNNNNGTG | 2 cut(s) | 804, 921 |
| PaeR7I | CTCGAG | 1 cut(s) | 2129 |
| PagI | TCATGA | 4 cut(s) | 100, 139, 372, 534 |
| PceI | AGGCCT | 2 cut(s) | 1576, 2293 |
| PciI | ACATGT | 2 cut(s) | 2036, 2150 |
| PciSI | GCTCTTC | 1 cut(s) | 1377 |
| PctI | GAATGC | 4 cut(s) | 1084, 1288, 1561, 2098 |
| PdmI | GAANNNNTTC | 4 cut(s) | 306, 468, 630, 1264 |
| PfeI | GAWTC | 8 cut(s) | 47, 1534, 1766, 1967, 2111, 2174, 2434, 2448 |
| PflFI | GACNNNGTC | 1 cut(s) | 1356 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2051 |
| PkrI | GCNGC | 3 cut(s) | 1168, 1297, 2529 |
| Ple19I | CGATCG | 1 cut(s) | 2419 |
| PleI | GAGTC | 1 cut(s) | 1201 |
| PpsI | GAGTC | 1 cut(s) | 1201 |
| PscI | ACATGT | 2 cut(s) | 2036, 2150 |
| PsiI | TTATAA | 2 cut(s) | 846, 2222 |
| Psp124BI | GAGCTC | 1 cut(s) | 1389 |
| Psp6I | CCWGG | 3 cut(s) | 1885, 2422, 2475 |
| PspGI | CCWGG | 3 cut(s) | 1885, 2422, 2475 |
| PspN4I | GGNNCC | 9 cut(s) | 7, 153, 243, 405, 567, 1029, 1368, 1737, 2139 |
| PspPI | GGNCC | 4 cut(s) | 6, 151, 1736, 2473 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 242, 274 |
| PstI | CTGCAG | 1 cut(s) | 1776 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1172, 2533 |
| PsuI | RGATCY | 9 cut(s) | 241, 403, 565, 1246, 1303, 1786, 2137, 2227, 2389 |
| PsyI | GACNNNGTC | 1 cut(s) | 1356 |
| PvuI | CGATCG | 1 cut(s) | 2419 |
| RgaI | GCGATCGC | 1 cut(s) | 2419 |
| RsaI | GTAC | 3 cut(s) | 2127, 2158, 2384 |
| RsaNI | GTAC | 3 cut(s) | 2126, 2157, 2383 |
| RseI | CAYNNNNRTG | 7 cut(s) | 99, 105, 144, 783, 804, 921, 957 |
| SacI | GAGCTC | 1 cut(s) | 1389 |
| SapI | GCTCTTC | 1 cut(s) | 1377 |
| SaqAI | TTAA | 9 cut(s) | 774, 942, 1241, 1715, 1748, 2120, 2166, 2208, 2232 |
| SatI | GCNGC | 3 cut(s) | 1167, 1296, 2528 |
| Sau96I | GGNCC | 4 cut(s) | 6, 151, 1736, 2473 |
| SchI | GAGTC | 1 cut(s) | 1201 |
| ScrFI | CCNGG | 3 cut(s) | 1887, 2424, 2477 |
| SduI | GDGCHC | 2 cut(s) | 1389, 2044 |
| SexAI | ACCWGGT | 1 cut(s) | 1885 |
| SfaAI | GCGATCGC | 1 cut(s) | 2419 |
| SfaNI | GCATC | 4 cut(s) | 209, 371, 533, 1135 |
| SfcI | CTRYAG | 3 cut(s) | 888, 1772, 1855 |
| Sfr274I | CTCGAG | 1 cut(s) | 2129 |
| SgfI | GCGATCGC | 1 cut(s) | 2419 |
| SinI | GGWCC | 3 cut(s) | 6, 151, 1736 |
| SlaI | CTCGAG | 1 cut(s) | 2129 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 99, 105, 144, 783, 804, 921, 957 |
| SmlI | CTYRAG | 5 cut(s) | 271, 763, 2012, 2129, 2261 |
| SmoI | CTYRAG | 5 cut(s) | 271, 763, 2012, 2129, 2261 |
| SseBI | AGGCCT | 2 cut(s) | 1576, 2293 |
| SsiI | CCGC | 8 cut(s) | 205, 367, 529, 691, 1945, 2096, 2325, 2443 |
| SspMI | CTAG | 8 cut(s) | 201, 228, 921, 981, 1158, 1301, 1545, 1809 |
| SstI | GAGCTC | 1 cut(s) | 1389 |
| StuI | AGGCCT | 2 cut(s) | 1576, 2293 |
| StyD4I | CCNGG | 3 cut(s) | 1885, 2422, 2475 |
| StyI | CCWWGG | 4 cut(s) | 200, 1008, 1731, 2495 |
| TaaI | ACNGT | 2 cut(s) | 978, 2535 |
| TaiI | ACGT | 1 cut(s) | 2239 |
| TaqI | TCGA | 2 cut(s) | 50, 2130 |
| TaqII | GACCGA | 1 cut(s) | 147 |
| TatI | WGTACW | 2 cut(s) | 2125, 2382 |
| TfiI | GAWTC | 8 cut(s) | 47, 1534, 1766, 1967, 2111, 2174, 2434, 2448 |
| Tru1I | TTAA | 9 cut(s) | 774, 942, 1241, 1715, 1748, 2120, 2166, 2208, 2232 |
| Tru9I | TTAA | 9 cut(s) | 774, 942, 1241, 1715, 1748, 2120, 2166, 2208, 2232 |
| TscAI | CASTG | 5 cut(s) | 627, 1177, 1275, 1506, 2352 |
| TseFI | GTSAC | 1 cut(s) | 1940 |
| TseI | GCWGC | 3 cut(s) | 1166, 1295, 2527 |
| Tsp45I | GTSAC | 1 cut(s) | 1940 |
| TspRI | CASTG | 5 cut(s) | 627, 1177, 1275, 1506, 2352 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1356 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2051 |
| VneI | GTGCAC | 1 cut(s) | 2040 |
| VpaK11BI | GGWCC | 3 cut(s) | 6, 151, 1736 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2298 |
| XapI | RAATTY | 8 cut(s) | 430, 610, 1054, 1186, 1478, 1527, 2204, 2314 |
| XbaI | TCTAGA | 1 cut(s) | 1300 |
| XceI | RCATGY | 2 cut(s) | 2040, 2154 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1045 |
| XhoI | CTCGAG | 1 cut(s) | 2129 |
| XmaJI | CCTAGG | 1 cut(s) | 200 |
| XmiI | GTMKAC | 1 cut(s) | 2056 |
| XmnI | GAANNNNTTC | 4 cut(s) | 306, 468, 630, 1264 |
| XspI | CTAG | 8 cut(s) | 201, 228, 921, 981, 1158, 1301, 1545, 1809 |
| Zsp2I | ATGCAT | 5 cut(s) | 224, 298, 386, 460, 548 |