Rorug06G0266400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000931
Physical Location & Seq
Forward (+)
41300263 .. 41303077
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0266400.1

Sequence Viewer

Length: 642 bp
ATGGCGGACGACGAAGCCGGAGGCGAGGAGTACTTGTTCAAGGTGGTCCTCATCGGCGACTCCGCCGTCGGCAAGTCCAACCTGCTGTCTCGGTTCGCTAGGAACGAGTTCGATCAAAACTCCAAGGCCACAATTGGGGTCGAGTTTCAGACCCAGGCTGTGGAAATCGACGGCAAAGAGGTCAAGGCCCAGATCTGGGACACGGCGGGGCAAGAGCGCTTCCGGGCTGTGACGTCTGCTTACTACAGAGGCGCGGTGGGCGCGCTTGTGGTGTATGATATCAGTAGGAAGACTACTTTTGAGAGTGTGGAGCGCTGGCTTAATGAGCTTACCATGCATTGTGATACTGCGGTGGCAAAGATGTTGGTAGGGAATAAGTGTGATTTGGAGGATATTAGAGATGTGTCCATTGAAGACGGTAAGAGCCTTGCAGAAGAAAAGGGACTATTCTTCATGGAGACTTCTGCACTTGATGCTACTAATGTTCAAGAGGCCTTTGAGGTTGTTATCCGGGAGATTTACAACAATGTCAGCAGGAAAGTCCTAAATTCTGATTCTTACAAGGCTGAGCTGTCTGTTAACCGAGTAACCCTGGTGAAAAATGGGGCAGATTCGACGAAATATCGGTGTTGTAGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.66

Weight (kDa)

4.83

Isoelectric Point (pI)

28.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 172 3.7e-15 ADP-ribosylation factor family
Ras PF00071 14 - 174 5.2e-64 Ras family
Roc PF08477 14 - 128 8.5e-36 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 14 - 111 8e-06 50S ribosome-binding GTPase
GTP_EFTU PF00009 45 - 173 6e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AatII GACGTC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 90
AccB7I CCANNNNNTGG 1 cut(s) 160
AccII CGCG 2 cut(s) 254, 263
AciI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
AcsI RAATTY 1 cut(s) 547
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 1 cut(s) 32
AfeI AGCGCT 2 cut(s) 218, 314
AfiI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
AgsI TTSAA 3 cut(s) 40, 413, 488
AjnI CCWGG 2 cut(s) 153, 591
AloI GAACNNNNNNTCC 2 cut(s) 20, 52
AluBI AGCT 2 cut(s) 328, 571
AluI AGCT 2 cut(s) 328, 571
Alw26I GTCTC 2 cut(s) 93, 452
Aor51HI AGCGCT 2 cut(s) 218, 314
AoxI GGCC 3 cut(s) 126, 186, 492
ApoI RAATTY 1 cut(s) 547
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 5 cut(s) 219, 254, 263, 265, 315
AspS9I GGNCC 2 cut(s) 46, 187
AsuC2I CCSGG 2 cut(s) 224, 512
AsuHPI GGTGA 1 cut(s) 607
AvaII GGWCC 1 cut(s) 46
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BbsI GAAGAC 2 cut(s) 296, 420
BceAI ACGGC 3 cut(s) 50, 187, 219
BciT130I CCWGG 2 cut(s) 155, 593
BcnI CCSGG 2 cut(s) 224, 512
BcoDI GTCTC 2 cut(s) 93, 452
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 244
BfoI RGCGCY 2 cut(s) 220, 316
BfuAI ACCTGC 1 cut(s) 90
BglII AGATCT 1 cut(s) 192
BlpI GCTNAGC 1 cut(s) 567
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 4 cut(s) 155, 224, 512, 593
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 2 cut(s) 46, 187
BmrFI CCNGG 4 cut(s) 155, 224, 512, 593
BmsI GCATC 1 cut(s) 463
BpiI GAAGAC 2 cut(s) 296, 420
Bpu1102I GCTNAGC 1 cut(s) 567
BpuMI CCSGG 2 cut(s) 224, 512
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 3 cut(s) 123, 153, 591
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BseBI CCWGG 2 cut(s) 155, 593
BseDI CCNNGG 3 cut(s) 123, 153, 591
BseLI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BseMII CTCAG 1 cut(s) 558
BsePI GCGCGC 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 450
Bsh1236I CGCG 2 cut(s) 254, 263
BshFI GGCC 3 cut(s) 128, 188, 494
BsiSI CCGG 3 cut(s) 18, 223, 511
BslFI GGGAC 2 cut(s) 212, 456
BslI CCNNNNNNNGG 4 cut(s) 135, 160, 195, 196
BsmAI GTCTC 2 cut(s) 93, 452
BsmFI GGGAC 2 cut(s) 212, 456
BsnI GGCC 3 cut(s) 128, 188, 494
Bsp143I GATC 2 cut(s) 112, 192
Bsp1720I GCTNAGC 1 cut(s) 567
BspACI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
BspANI GGCC 3 cut(s) 128, 188, 494
BspCNI CTCAG 1 cut(s) 559
BspFNI CGCG 2 cut(s) 254, 263
BspMI ACCTGC 1 cut(s) 90
BssECI CCNNGG 3 cut(s) 123, 153, 591
BssHII GCGCGC 1 cut(s) 261
BssMI GATC 2 cut(s) 112, 192
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 155, 593
Bst4CI ACNGT 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 233
BstAPI GCANNNNNTGC 1 cut(s) 473
BstC8I GCNNGC 2 cut(s) 263, 317
BstDEI CTNAG 1 cut(s) 567
BstFNI CGCG 2 cut(s) 254, 263
BstH2I RGCGCY 2 cut(s) 220, 316
BstHHI GCGC 5 cut(s) 219, 254, 263, 265, 315
BstKTI GATC 2 cut(s) 115, 195
BstMAI GTCTC 2 cut(s) 93, 452
BstMBI GATC 2 cut(s) 112, 192
BstMWI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
BstNI CCWGG 2 cut(s) 155, 593
BstSCI CCNGG 4 cut(s) 153, 222, 510, 591
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 2 cut(s) 254, 263
BstV2I GAAGAC 2 cut(s) 296, 420
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 3 cut(s) 128, 188, 494
BveI ACCTGC 1 cut(s) 90
Cac8I GCNNGC 2 cut(s) 263, 317
CfoI GCGC 5 cut(s) 219, 254, 263, 265, 315
Cfr13I GGNCC 2 cut(s) 46, 187
Csp6I GTAC 1 cut(s) 31
CviAII CATG 2 cut(s) 334, 454
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 1 cut(s) 567
DpnI GATC 2 cut(s) 114, 194
DpnII GATC 2 cut(s) 112, 192
DrdI GACNNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 65
EciI GGCGGA 2 cut(s) 20, 52
Eco130I CCWWGG 1 cut(s) 123
Eco147I AGGCCT 1 cut(s) 494
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 2 cut(s) 218, 314
EcoRII CCWGG 2 cut(s) 153, 591
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 337, 457
FaiI YATR 3 cut(s) 276, 335, 455
FaqI GGGAC 2 cut(s) 212, 456
FatI CATG 2 cut(s) 333, 453
FauI CCCGC 1 cut(s) 199
FspBI CTAG 1 cut(s) 99
GlaI GCGC 5 cut(s) 218, 253, 262, 264, 314
HaeII RGCGCY 2 cut(s) 220, 316
HaeIII GGCC 3 cut(s) 128, 188, 494
HapII CCGG 3 cut(s) 18, 223, 511
HhaI GCGC 5 cut(s) 219, 254, 263, 265, 315
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 2 cut(s) 337, 457
Hin6I GCGC 5 cut(s) 217, 252, 261, 263, 313
HinP1I GCGC 5 cut(s) 217, 252, 261, 263, 313
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HinfI GANTC 3 cut(s) 59, 554, 611
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 3 cut(s) 18, 223, 511
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 1 cut(s) 580
Hpy188I TCNGA 2 cut(s) 150, 553
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 1 cut(s) 580
Hpy99I CGWCG 4 cut(s) 14, 71, 173, 619
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 3 cut(s) 337, 431, 467
HpyF10VI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
HpyF3I CTNAG 1 cut(s) 567
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 2 cut(s) 337, 457
HspAI GCGC 5 cut(s) 217, 252, 261, 263, 313
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 112, 192
LmnI GCTCC 1 cut(s) 310
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 229, 586
MalI GATC 2 cut(s) 114, 194
MboI GATC 2 cut(s) 112, 192
MboII GAAGA 4 cut(s) 301, 425, 442, 446
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 1 cut(s) 192
MluCI AATT 2 cut(s) 132, 547
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 8 cut(s) 14, 19, 59, 172, 242, 382, 484, 493
Mph1103I ATGCAT 1 cut(s) 339
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 2 cut(s) 321, 579
MspI CCGG 3 cut(s) 18, 223, 511
MspR9I CCNGG 4 cut(s) 155, 224, 512, 593
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 2 cut(s) 155, 593
MvnI CGCG 2 cut(s) 254, 263
MwoI GCNNNNNNNGC 5 cut(s) 258, 260, 325, 334, 473
NciI CCSGG 2 cut(s) 224, 512
NdeII GATC 2 cut(s) 112, 192
NlaIII CATG 2 cut(s) 337, 457
NmuCI GTSAC 1 cut(s) 229
NsiI ATGCAT 1 cut(s) 339
PauI GCGCGC 1 cut(s) 261
PceI AGGCCT 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 2 cut(s) 554, 611
PflMI CCANNNNNTGG 1 cut(s) 160
PfoI TCCNGGA 1 cut(s) 510
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 153, 591
PspGI CCWGG 2 cut(s) 153, 591
PspPI GGNCC 2 cut(s) 46, 187
PsuI RGATCY 1 cut(s) 192
PteI GCGCGC 1 cut(s) 261
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SaqAI TTAA 2 cut(s) 321, 579
Sau3AI GATC 2 cut(s) 112, 192
Sau96I GGNCC 2 cut(s) 46, 187
ScaI AGTACT 1 cut(s) 32
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 4 cut(s) 155, 224, 512, 593
SetI ASST 7 cut(s) 45, 84, 183, 236, 330, 504, 573
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 1 cut(s) 46
Sse9I AATT 2 cut(s) 132, 547
SseBI AGGCCT 1 cut(s) 494
SsiI CCGC 6 cut(s) 5, 63, 206, 254, 350, 636
SspMI CTAG 1 cut(s) 99
StuI AGGCCT 1 cut(s) 494
StyD4I CCNGG 4 cut(s) 153, 222, 510, 591
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 419
TaiI ACGT 1 cut(s) 236
TaqI TCGA 4 cut(s) 111, 141, 168, 614
TasI AATT 2 cut(s) 132, 547
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 2 cut(s) 554, 611
Tru1I TTAA 2 cut(s) 321, 579
Tru9I TTAA 2 cut(s) 321, 579
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 442
Van91I CCANNNNNTGG 1 cut(s) 160
VpaK11BI GGWCC 1 cut(s) 46
XapI RAATTY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 99
ZraI GACGTC 1 cut(s) 234
ZrmI AGTACT 1 cut(s) 32
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.