FvH4_2g28620

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012579
Physical Location & Seq
Reverse (-)
22378852 .. 22380348
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28620.t1

Sequence Viewer

Length: 525 bp
ATGGCTGACAGTGCTGCTTACAGACGTCTGATTTCCTCCGTGATCAAACTTCTTGACTCGAACAATTACCATGAATCTGCTCGCACGCTGGAAAAAGAATCTGGCTTCGTTTTCAACCTGGGGTACTTTGAACACTGTTTTGTGACGGGACTGCTCGAAGAGGCTGAGGACTACCTCGCCGGGTTTTTGCCGTACATACACGAAAACGCCTACTGTGTCAATGTGTTTTTTGAGCTCAATTGGCAGAAGTTCATGGATGTGCTCGACCAGGGCGACCGGATAGAGGCGAGAAGAATCTTGGATGAAGATCTACGACCGTTCGAGCGGTACCAGCCCGACCTGATCGTTTCGGCAAGTGAGTTAGCGAGCTTGGAGGATTTCAGGACGCATGAGGCGTTTATGAATTATGGAGTTCCTGAAGAGGAGAGAAGGGAGAGGTTGGAGTTCGTGAGGACCAATTTGGAGGCAAATCCTCTTCTCGACGGAAAGATTCAATATGATCCCCCGGCACCTCCTCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

175

Amino Acids

20.29

Weight (kDa)

4.56

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR1-like_2nd PF21889 39 - 164 6.5e-24 TPR1-like, CTLH-containing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 28
Acc65I GGTACC 1 cut(s) 327
AccB1I GGYRCC 2 cut(s) 327, 508
AccBSI CCGCTC 1 cut(s) 325
AciI CCGC 1 cut(s) 325
AclWI GGATC 1 cut(s) 494
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 25
AfaI GTAC 3 cut(s) 125, 194, 329
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 3 cut(s) 115, 131, 494
AjnI CCWGG 2 cut(s) 117, 267
AluBI AGCT 3 cut(s) 235, 369, 521
AluI AGCT 3 cut(s) 235, 369, 521
Alw21I GWGCWC 2 cut(s) 237, 264
AlwI GGATC 1 cut(s) 494
ApeKI GCWGC 1 cut(s) 14
Asp718I GGTACC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 453
AsuC2I CCSGG 2 cut(s) 181, 506
AvaII GGWCC 1 cut(s) 453
BanI GGYRCC 2 cut(s) 327, 508
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 2 cut(s) 237, 264
BbvCI CCTCAGC 1 cut(s) 165
BceAI ACGGC 1 cut(s) 175
BciT130I CCWGG 2 cut(s) 119, 269
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 2 cut(s) 181, 506
BglII AGATCT 1 cut(s) 307
BisI GCNGC 1 cut(s) 15
BlsI GCNGC 1 cut(s) 16
Bme1390I CCNGG 4 cut(s) 119, 181, 269, 506
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 329, 510
BmrFI CCNGG 4 cut(s) 119, 181, 269, 506
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 2 cut(s) 181, 506
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 25
BsaJI CCNNGG 3 cut(s) 118, 268, 504
BsaWI WCCGGW 1 cut(s) 276
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 2 cut(s) 119, 269
BseDI CCNNGG 3 cut(s) 118, 268, 504
BseGI GGATG 2 cut(s) 262, 307
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 156
BseRI GAGGAG 2 cut(s) 437, 504
Bsh1285I CGRYCG 2 cut(s) 277, 317
BshNI GGYRCC 2 cut(s) 327, 508
BsiEI CGRYCG 2 cut(s) 277, 317
BsiHKAI GWGCWC 2 cut(s) 237, 264
BsiSI CCGG 3 cut(s) 180, 277, 506
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 283
BsmFI GGGAC 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 237, 264
Bsp143I GATC 4 cut(s) 42, 307, 342, 499
BspACI CCGC 1 cut(s) 325
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 329, 510
BspPI GGATC 1 cut(s) 494
BspT107I GGYRCC 2 cut(s) 327, 508
BsrBI CCGCTC 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 118, 268, 504
BssMI GATC 4 cut(s) 42, 307, 342, 499
BssNI GRCGYC 1 cut(s) 25
Bst2UI CCWGG 2 cut(s) 119, 269
Bst4CI ACNGT 4 cut(s) 11, 137, 215, 318
Bst6I CTCTTC 3 cut(s) 153, 414, 480
BstACI GRCGYC 1 cut(s) 25
BstC8I GCNNGC 3 cut(s) 82, 86, 367
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 262, 307
BstKTI GATC 4 cut(s) 45, 310, 345, 502
BstMBI GATC 4 cut(s) 42, 307, 342, 499
BstMCI CGRYCG 2 cut(s) 277, 317
BstMWI GCNNNNNNNGC 3 cut(s) 11, 241, 331
BstNI CCWGG 2 cut(s) 119, 269
BstSCI CCNGG 4 cut(s) 117, 179, 267, 504
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BtsCI GGATG 2 cut(s) 262, 307
BtsIMutI CAGTG 2 cut(s) 16, 133
Cac8I GCNNGC 3 cut(s) 82, 86, 367
Cfr13I GGNCC 1 cut(s) 453
CseI GACGC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 124, 193, 328
CviAII CATG 3 cut(s) 71, 253, 389
CviJI RGCY 7 cut(s) 5, 105, 164, 235, 334, 369, 521
CviKI_1 RGCY 7 cut(s) 5, 105, 164, 235, 334, 369, 521
CviQI GTAC 3 cut(s) 124, 193, 328
DdeI CTNAG 1 cut(s) 165
DpnI GATC 4 cut(s) 44, 309, 344, 501
DpnII GATC 4 cut(s) 42, 307, 342, 499
Eam1104I CTCTTC 3 cut(s) 153, 414, 480
EarI CTCTTC 3 cut(s) 153, 414, 480
Ecl136II GAGCTC 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 453
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 438
EcoICRI GAGCTC 1 cut(s) 235
EcoRII CCWGG 2 cut(s) 117, 267
EcoT38I GRGCYC 1 cut(s) 237
FaeI CATG 3 cut(s) 74, 256, 392
FaiI YATR 7 cut(s) 72, 197, 254, 390, 401, 408, 498
FaqI GGGAC 1 cut(s) 162
FatI CATG 3 cut(s) 70, 252, 388
FbaI TGATCA 1 cut(s) 42
Fnu4HI GCNGC 1 cut(s) 15
FokI GGATG 2 cut(s) 269, 314
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 15
GluI GCNGC 1 cut(s) 15
HapII CCGG 3 cut(s) 180, 277, 506
HgaI GACGC 1 cut(s) 394
Hin1I GRCGYC 1 cut(s) 25
Hin1II CATG 3 cut(s) 74, 256, 392
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 5 cut(s) 56, 74, 98, 294, 490
HpaII CCGG 3 cut(s) 180, 277, 506
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 5 cut(s) 53, 382, 416, 448, 479
Hpy99I CGWCG 1 cut(s) 485
HpyAV CCTTC 1 cut(s) 423
HpyCH4III ACNGT 4 cut(s) 11, 137, 215, 318
HpyCH4IV ACGT 1 cut(s) 25
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 241, 331
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 25
Hsp92I GRCGYC 1 cut(s) 25
Hsp92II CATG 3 cut(s) 74, 256, 392
KpnI GGTACC 1 cut(s) 331
Ksp22I TGATCA 1 cut(s) 42
Kzo9I GATC 4 cut(s) 42, 307, 342, 499
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 142
MalI GATC 4 cut(s) 44, 309, 344, 501
MbiI CCGCTC 1 cut(s) 325
MboI GATC 4 cut(s) 42, 307, 342, 499
MboII GAAGA 5 cut(s) 170, 303, 317, 431, 467
MfeI CAATTG 1 cut(s) 238
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 2 cut(s) 237, 264
MluCI AATT 4 cut(s) 64, 238, 403, 457
MlyI GAGTC 1 cut(s) 50
MmeI TCCRAC 1 cut(s) 420
MslI CAYNNNNRTG 1 cut(s) 257
MspI CCGG 3 cut(s) 180, 277, 506
MspR9I CCNGG 4 cut(s) 119, 181, 269, 506
MunI CAATTG 1 cut(s) 238
MvaI CCWGG 2 cut(s) 119, 269
MwoI GCNNNNNNNGC 3 cut(s) 11, 241, 331
NciI CCSGG 2 cut(s) 181, 506
NdeII GATC 4 cut(s) 42, 307, 342, 499
NlaIII CATG 3 cut(s) 74, 256, 392
NlaIV GGNNCC 2 cut(s) 329, 510
NmuCI GTSAC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 2 cut(s) 270, 392
PfeI GAWTC 4 cut(s) 74, 98, 294, 490
PkrI GCNGC 1 cut(s) 16
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 117, 267
PspGI CCWGG 2 cut(s) 117, 267
PspN4I GGNNCC 2 cut(s) 329, 510
PspPI GGNCC 1 cut(s) 453
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 3 cut(s) 125, 194, 329
RsaNI GTAC 3 cut(s) 124, 193, 328
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 237
SatI GCNGC 1 cut(s) 15
Sau3AI GATC 4 cut(s) 42, 307, 342, 499
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 1 cut(s) 50
ScrFI CCNGG 4 cut(s) 119, 181, 269, 506
SduI GDGCHC 2 cut(s) 237, 264
SetI ASST 9 cut(s) 28, 120, 177, 237, 342, 371, 440, 514, 523
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 4 cut(s) 64, 238, 403, 457
SsiI CCGC 1 cut(s) 325
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 4 cut(s) 117, 179, 267, 504
TaaI ACNGT 4 cut(s) 11, 137, 215, 318
TaiI ACGT 1 cut(s) 28
TaqI TCGA 5 cut(s) 59, 156, 264, 321, 480
TasI AATT 4 cut(s) 64, 238, 403, 457
TfiI GAWTC 4 cut(s) 74, 98, 294, 490
TscAI CASTG 2 cut(s) 16, 140
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 4 cut(s) 87, 241, 318, 416
TspGWI ACGGA 2 cut(s) 28, 498
TspRI CASTG 2 cut(s) 16, 140
VpaK11BI GGWCC 1 cut(s) 453
ZraI GACGTC 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.