FvH4_2g28630

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012579
Physical Location & Seq
Reverse (-)
22387620 .. 22389391
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g28630.t1

Sequence Viewer

Length: 687 bp
ATGGTTGTTCCCAACGCACAGAGAGATGCGATTGACTCTATTGTTCAATATCTCGACAGAATCAATCTGAAGGAATCCGCAAGAATGCTGGAGCGTGAATCTGGTTTATTCTTTAATGTGGCTTATTTTGAAGACTGTTTTCTTCGTGGAGCCTTCGAAGCCGCGGAGGACTACCTGACGGGCTTCACCGGAGTCCATGACAATAATTTTACAATCAATGTCGTATTTGAGCTCCGGTGGCACAAATTCTTGGCCGCCCTAAACGACAATAATCGTGAGGAAGCTCGAGTCATCATCCGTGAGAAACTTCAACCATTGGCGGTCTACAACAAAGATTTGCTAGGGTTTGCCAACACTCTAGTGAATATGCATAACTTCAGGATGCATGAAGCTTTCTTGTTCTACGGGGAACCACAGTACCACAGGATGGAGAGGCTGGAACTCATTAGGATGAACTTGAACTCCAATCCTCTGTTGCATGGAAAGCTTCGGTATGCCATTCACAGCGTGTATAATTCACTGGTCATAGCTCCACCGATTCCAGCTGCTCTGGCAGTTCCTGCCGCTTCACTAGTTCCAATTGGTCCAGCACCTCGAGCAGCTCCCCAGCCATTAGCGGTACCAGCAGGACCGAGGAACTATACTACTGTTACTCCAAGCTGGTTTCCTAGACCTCGGAGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04137 ko04140 map04137 map04140
Pfam Domains
Protein Families
WD40

Protein Analysis

229

Amino Acids

26.02

Weight (kDa)

8.71

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR1-like_2nd PF21889 39 - 163 1.6e-24 TPR1-like, CTLH-containing domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 619
AccB7I CCANNNNNTGG 1 cut(s) 427
AccI GTMKAC 1 cut(s) 324
AccII CGCG 1 cut(s) 164
AciI CCGC 7 cut(s) 78, 162, 164, 255, 320, 564, 617
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 2 cut(s) 89, 361
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 2 cut(s) 419, 621
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 4 cut(s) 47, 131, 311, 460
AhlI ACTAGT 1 cut(s) 571
AleI CACNNNNGTG 1 cut(s) 359
AloI GAACNNNNNNTCC 2 cut(s) 446, 478
AluBI AGCT 8 cut(s) 232, 284, 392, 487, 530, 545, 602, 660
AluI AGCT 8 cut(s) 232, 284, 392, 487, 530, 545, 602, 660
Alw21I GWGCWC 1 cut(s) 234
AlwNI CAGNNNCTG 1 cut(s) 560
Ama87I CYCGRG 2 cut(s) 285, 594
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 2 cut(s) 545, 599
ApoI RAATTY 1 cut(s) 245
Asp718I GGTACC 1 cut(s) 619
AspS9I GGNCC 2 cut(s) 584, 629
AsuHPI GGTGA 1 cut(s) 178
AsuII TTCGAA 1 cut(s) 156
AvaI CYCGRG 2 cut(s) 285, 594
AvaII GGWCC 2 cut(s) 584, 629
BaeI ACNNNNGTAYC 2 cut(s) 401, 434
BanI GGYRCC 1 cut(s) 619
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 532, 611
BccI CCATC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 571
BfaI CTAG 4 cut(s) 341, 359, 572, 669
BisI GCNGC 5 cut(s) 162, 255, 546, 564, 600
BlsI GCNGC 5 cut(s) 163, 256, 547, 565, 601
Bme18I GGWCC 2 cut(s) 584, 629
BmeT110I CYCGRG 2 cut(s) 285, 594
BmgT120I GGNCC 2 cut(s) 584, 629
BmiI GGNNCC 3 cut(s) 151, 411, 621
BmsI GCATC 2 cut(s) 16, 372
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 156
BsaJI CCNNGG 3 cut(s) 162, 632, 674
BsaWI WCCGGW 2 cut(s) 188, 234
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 525
BseDI CCNNGG 3 cut(s) 162, 632, 674
BseGI GGATG 4 cut(s) 294, 387, 432, 456
BseLI CCNNNNNNNGG 1 cut(s) 427
BseNI ACTGG 1 cut(s) 525
BseXI GCAGC 2 cut(s) 532, 611
BseYI CCCAGC 1 cut(s) 606
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 1 cut(s) 254
BshNI GGYRCC 1 cut(s) 619
BsiHKAI GWGCWC 1 cut(s) 234
BsiHKCI CYCGRG 2 cut(s) 285, 594
BsiSI CCGG 2 cut(s) 189, 235
BslI CCNNNNNNNGG 1 cut(s) 427
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 1 cut(s) 254
BsoBI CYCGRG 2 cut(s) 285, 594
Bsp119I TTCGAA 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 234
BspACI CCGC 7 cut(s) 78, 162, 164, 255, 320, 564, 617
BspANI GGCC 1 cut(s) 254
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 3 cut(s) 151, 411, 621
BspT104I TTCGAA 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 619
BsrI ACTGG 1 cut(s) 525
BssECI CCNNGG 3 cut(s) 162, 632, 674
Bst4CI ACNGT 3 cut(s) 137, 417, 649
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 4 cut(s) 294, 387, 432, 456
BstFNI CGCG 1 cut(s) 164
BstMWI GCNNNNNNNGC 7 cut(s) 158, 238, 484, 551, 560, 596, 623
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 532, 611
BstV2I GAAGAC 1 cut(s) 138
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 4 cut(s) 294, 387, 432, 456
BtsIMutI CAGTG 1 cut(s) 518
CaiI CAGNNNCTG 1 cut(s) 560
Cfr13I GGNCC 2 cut(s) 584, 629
Cfr42I CCGCGG 1 cut(s) 165
Csp6I GTAC 2 cut(s) 418, 620
CviAII CATG 3 cut(s) 197, 386, 479
CviQI GTAC 2 cut(s) 418, 620
DraIII CACNNNGTG 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 584, 629
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 89, 361
Eco88I CYCGRG 2 cut(s) 285, 594
EcoICRI GAGCTC 1 cut(s) 232
EcoT22I ATGCAT 2 cut(s) 372, 387
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 3 cut(s) 200, 389, 482
FaiI YATR 9 cut(s) 198, 368, 372, 387, 480, 495, 513, 527, 642
FatI CATG 3 cut(s) 196, 385, 478
FblI GTMKAC 1 cut(s) 324
Fnu4HI GCNGC 5 cut(s) 162, 255, 546, 564, 600
FokI GGATG 4 cut(s) 281, 394, 439, 463
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 162, 255, 546, 564, 600
FspBI CTAG 4 cut(s) 341, 359, 572, 669
GluI GCNGC 5 cut(s) 162, 255, 546, 564, 600
GsaI CCCAGC 1 cut(s) 610
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 1 cut(s) 254
HapII CCGG 2 cut(s) 189, 235
Hin1II CATG 3 cut(s) 200, 389, 482
HindIII AAGCTT 2 cut(s) 390, 485
HinfI GANTC 7 cut(s) 35, 60, 74, 98, 192, 288, 538
HpaII CCGG 2 cut(s) 189, 235
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 69, 678
Hpy188III TCNNGA 3 cut(s) 53, 275, 379
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 64, 163
HpyCH4III ACNGT 3 cut(s) 137, 417, 649
HpyCH4V TGCA 3 cut(s) 370, 385, 478
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 238, 484, 551, 560, 596, 623
Hsp92II CATG 3 cut(s) 200, 389, 482
KpnI GGTACC 1 cut(s) 623
KspI CCGCGG 1 cut(s) 165
LmnI GCTCC 5 cut(s) 91, 149, 237, 535, 607
Lsp1109I GCAGC 2 cut(s) 532, 611
LweI GCATC 2 cut(s) 16, 372
MaeI CTAG 4 cut(s) 341, 359, 572, 669
MaeIII GTNAC 1 cut(s) 649
MboII GAAGA 2 cut(s) 134, 143
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 4 cut(s) 205, 245, 514, 579
MlyI GAGTC 3 cut(s) 29, 201, 297
MnlI CCTC 8 cut(s) 160, 271, 426, 480, 603, 627, 672, 684
Mph1103I ATGCAT 2 cut(s) 372, 387
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 2 cut(s) 359, 449
MspA1I CMGCKG 2 cut(s) 164, 545
MspI CCGG 2 cut(s) 189, 235
MunI CAATTG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 90
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 7 cut(s) 158, 238, 484, 551, 560, 596, 623
NlaIII CATG 3 cut(s) 200, 389, 482
NlaIV GGNNCC 3 cut(s) 151, 411, 621
NsiI ATGCAT 2 cut(s) 372, 387
NspV TTCGAA 1 cut(s) 156
OliI CACNNNNGTG 1 cut(s) 359
PaeR7I CTCGAG 2 cut(s) 285, 594
PctI GAATGC 1 cut(s) 90
PfeI GAWTC 4 cut(s) 60, 74, 98, 538
PflMI CCANNNNNTGG 1 cut(s) 427
PkrI GCNGC 5 cut(s) 163, 256, 547, 565, 601
PleI GAGTC 3 cut(s) 29, 200, 296
PpsI GAGTC 3 cut(s) 29, 200, 296
Psp124BI GAGCTC 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 606
PspN4I GGNNCC 3 cut(s) 151, 411, 621
PspPI GGNCC 2 cut(s) 584, 629
PspXI VCTCGAGB 2 cut(s) 285, 594
PstNI CAGNNNCTG 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 2 cut(s) 419, 621
RsaNI GTAC 2 cut(s) 418, 620
RseI CAYNNNNRTG 2 cut(s) 359, 449
SacI GAGCTC 1 cut(s) 234
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 5 cut(s) 162, 255, 546, 564, 600
Sau96I GGNCC 2 cut(s) 584, 629
SchI GAGTC 3 cut(s) 29, 201, 297
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 2 cut(s) 16, 372
Sfr274I CTCGAG 2 cut(s) 285, 594
Sfr303I CCGCGG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 2 cut(s) 584, 629
SlaI CTCGAG 2 cut(s) 285, 594
SmiMI CAYNNNNRTG 2 cut(s) 359, 449
SmlI CTYRAG 2 cut(s) 285, 594
SmoI CTYRAG 2 cut(s) 285, 594
SpeI ACTAGT 1 cut(s) 571
Sse9I AATT 4 cut(s) 205, 245, 514, 579
SsiI CCGC 7 cut(s) 78, 162, 164, 255, 320, 564, 617
SspMI CTAG 4 cut(s) 341, 359, 572, 669
SstI GAGCTC 1 cut(s) 234
TaaI ACNGT 3 cut(s) 137, 417, 649
TaqI TCGA 4 cut(s) 54, 156, 286, 595
TaqII GACCGA 1 cut(s) 646
TasI AATT 4 cut(s) 205, 245, 514, 579
TauI GCSGC 3 cut(s) 164, 257, 566
TfiI GAWTC 4 cut(s) 60, 74, 98, 538
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 525
TseI GCWGC 2 cut(s) 545, 599
TspDTI ATGAA 2 cut(s) 402, 467
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 1 cut(s) 525
Van91I CCANNNNNTGG 1 cut(s) 427
VpaK11BI GGWCC 2 cut(s) 584, 629
XapI RAATTY 1 cut(s) 245
XhoI CTCGAG 2 cut(s) 285, 594
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 4 cut(s) 341, 359, 572, 669
Zsp2I ATGCAT 2 cut(s) 372, 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.