FvH4_2g29790

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012103
Physical Location & Seq
Forward (+)
23028251 .. 23033916
5666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29790.t1

Sequence Viewer

Length: 1263 bp
ATGACTCTGGAAAAGAAGCCAAAGGCAGTGATAGTAGGAGGGAGCATAGGAGGTGTATCCTGCGCACACACGCTCATATTAGCAGGGTGGGATGTGGTGGTGCTTGAGAAGTCATATCAAGCCCCAACTGGAAGCCCTACTGGTGCAGGACTAGGACTTGACCCTCTCTCCTTGCAGCTCATTCAGTCATGGATCAAAGACCCTGAGCTTCTCAAGAGGACCACTCTACCCCTCACAATTGATCAGAATGATGCTGTGGATGGTGAGAAGAAGGTTAGGTGGACACTGACCAGAGATGAGAAGTTTAACTGCAGAGCAGCACATTGGGCTGACCTACATGCTCTTCTATATCATGCTTTGCCACCTAACTTGTTTCTTTGGGGTCACCATTTCCAGTCTTTCTCCATTTCTAGTGACAAGAGATCAGTTCAAGTGAAGGCTTTATCCCTTCAAAGTAATGAGACCGTCGAAGTCATTGGCGATTTGCTTGTTGCAGCAGATGGGTGTCTCTCAAAAATCCGCCAAAGTTTTGTTCCTGATCATAAGCTGAGGTATTCAGGATATTGTGCATGGAGAGGGGTTCTTGACTTTTCAGGAAGTGAGAAATCTGAAACTATAGTGGGGATCCGAAAGGCGTACCCTGAACTTGGGAAATGCTTGTACTTCGGCTTAGGTTCTGGAAGTCACACTGTGCTATATGAGCTTCTGAACAAAAGGCTGAATTGGATTTGGTATGTCCATCAACCAGAGCCTGATCTGAAGAGCGACTCAATGACCATGAAGGCAAACAGTGACATGATCCAGGCGATGCATAAAGAAGCAGAGAAGATGTGGCTTCCCGAGTTTGTGAGAGTCATCAAAGAAACAAGCCATCCTTTCATCAATGCCATATATGATAGTGAACCCTTGGAGCAAATCTATTGGGACAATGTGGTATTGGTTGGAGATGCAGCTCACCCCACAACTCCTCATGCTACAAGAAGCACAAACATGTCGGTATTGGATGCTGCGGTTTTAGGCCAGTGCCTGAAGAAGTGGGGTGCAGAAAACTTGCAATCAGCCCTTGAAGAATATCAGTCCATTCGGTTACCAGTCGTATCAAAACAAGTCCTTCATGCAAGGAGAATGGGGCGTATTAAACAAGGTCTAGCTCTTCCTGATCTGCAGTCTTTCGACCCCAAGACAGCTAATCCAGAGGAGTGTCAAGAGCTTCAACAGAGACTAATGCCTTTTTTCTCTGATGTTCCTTCCATACTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3 NAD_binding_8

Protein Analysis

421

Amino Acids

46.98

Weight (kDa)

6.54

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 9 - 176 1.9e-06 FAD binding domain
FAD_binding_3 PF01494 306 - 370 3.6e-06 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11330 AT5G11330
fragaria_vesca FvH4_2g29790 FvH4_2g29790
malus_domestica MD08G1141800.v1.1 MD15G1118300.v1.1 MD15G1335000.v1.1
prunus_persica Prupe.1G471700_v2.0.a1
pyrus_communis pycom15g10650
rosa_chinensis RchiOBHm_Chr6g0298811
rosa_laevigata RLG00000011483
rosa_multiflora Rmu_co8348659.1_g000001
rosa_roxburghii Rroxscaffold_7G00169310
rosa_rugosa Rorug06G0283000
rosa_samantha Rh6AG394700 Rh6BG402600 Rh6CG408300 Rh6DG395100
rosa_wichuraiana Rw6G034530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 64
AciI CCGC 2 cut(s) 520, 1010
AclWI GGATC 4 cut(s) 200, 619, 632, 793
AcuI CTGAAG 2 cut(s) 779, 1049
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 2 cut(s) 638, 662
AfiI CCNNNNNNNGG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 4 cut(s) 431, 452, 1067, 1214
AhlI ACTAGT 1 cut(s) 1255
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 516, 548
AluBI AGCT 8 cut(s) 178, 208, 547, 703, 953, 1151, 1187, 1210
AluI AGCT 8 cut(s) 178, 208, 547, 703, 953, 1151, 1187, 1210
Alw26I GTCTC 3 cut(s) 455, 512, 1213
AlwI GGATC 4 cut(s) 200, 619, 632, 793
AlwNI CAGNNNCTG 2 cut(s) 752, 1027
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 1 cut(s) 1018
ApeKI GCWGC 5 cut(s) 175, 317, 494, 950, 1007
AspLEI GCGC 1 cut(s) 65
AspS9I GGNCC 1 cut(s) 219
AsuHPI GGTGA 3 cut(s) 275, 377, 947
AvaI CYCGRG 1 cut(s) 839
AvaII GGWCC 1 cut(s) 219
BamHI GGATCC 1 cut(s) 624
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 5 cut(s) 187, 329, 506, 962, 994
BccI CCATC 4 cut(s) 254, 494, 747, 879
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 1 cut(s) 67
BclI TGATCA 2 cut(s) 241, 538
BcoDI GTCTC 3 cut(s) 455, 512, 1213
BcuI ACTAGT 1 cut(s) 1255
BfaI CTAG 4 cut(s) 152, 411, 1148, 1256
BfmI CTRYAG 3 cut(s) 310, 615, 1163
BfuI GTATCC 1 cut(s) 67
BisI GCNGC 5 cut(s) 176, 318, 495, 951, 1008
BlsI GCNGC 5 cut(s) 177, 319, 496, 952, 1009
Bme1390I CCNGG 1 cut(s) 803
Bme18I GGWCC 1 cut(s) 219
BmeT110I CYCGRG 1 cut(s) 839
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 1 cut(s) 803
BmsI GCATC 4 cut(s) 241, 798, 937, 994
BplI GAGNNNNNCTC 2 cut(s) 208, 240
Bpu10I CCTNAGC 3 cut(s) 204, 548, 670
BpuEI CTTGAG 2 cut(s) 125, 197
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 906
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse1I ACTGG 5 cut(s) 133, 145, 394, 1021, 1091
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 1 cut(s) 906
BseGI GGATG 4 cut(s) 97, 265, 871, 1009
BseLI CCNNNNNNNGG 1 cut(s) 647
BseMII CTCAG 2 cut(s) 195, 539
BseNI ACTGG 5 cut(s) 133, 145, 394, 1021, 1091
BseRI GAGGAG 2 cut(s) 957, 1211
BseXI GCAGC 5 cut(s) 187, 329, 506, 962, 994
BsgI GTGCAG 2 cut(s) 165, 1062
BshFI GGCC 1 cut(s) 1020
BsiHKCI CYCGRG 1 cut(s) 839
BslFI GGGAC 1 cut(s) 938
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 3 cut(s) 455, 512, 1213
BsmFI GGGAC 1 cut(s) 938
BsnI GGCC 1 cut(s) 1020
Bso31I GGTCTC 1 cut(s) 455
BsoBI CYCGRG 1 cut(s) 839
Bsp143I GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
BspACI CCGC 2 cut(s) 520, 1010
BspANI GGCC 1 cut(s) 1020
BspCNI CTCAG 2 cut(s) 196, 540
BspLI GGNNCC 1 cut(s) 626
BspMAI CTGCAG 2 cut(s) 314, 1167
BspPI GGATC 4 cut(s) 200, 619, 632, 793
BspQI GCTCTTC 3 cut(s) 348, 755, 1158
BspTNI GGTCTC 1 cut(s) 455
BsrI ACTGG 5 cut(s) 133, 145, 394, 1021, 1091
BssECI CCNNGG 1 cut(s) 906
BssMI GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
BssT1I CCWWGG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 3 cut(s) 466, 691, 791
Bst6I CTCTTC 3 cut(s) 348, 755, 1158
BstDEI CTNAG 3 cut(s) 204, 548, 670
BstEII GGTNACC 2 cut(s) 383, 1086
BstF5I GGATG 4 cut(s) 97, 265, 871, 1009
BstHHI GCGC 1 cut(s) 65
BstKTI GATC 8 cut(s) 195, 244, 425, 541, 627, 757, 801, 1162
BstMAI GTCTC 3 cut(s) 455, 512, 1213
BstMBI GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
BstMWI GCNNNNNNNGC 2 cut(s) 326, 700
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 2 cut(s) 341, 994
BstPI GGTNACC 2 cut(s) 383, 1086
BstSCI CCNGG 1 cut(s) 801
BstSFI CTRYAG 3 cut(s) 310, 615, 1163
BstV1I GCAGC 5 cut(s) 187, 329, 506, 962, 994
BstX2I RGATCY 1 cut(s) 624
BstYI RGATCY 1 cut(s) 624
BsuI GTATCC 1 cut(s) 67
BsuRI GGCC 1 cut(s) 1020
BtgZI GCGATG 1 cut(s) 821
BtsCI GGATG 4 cut(s) 97, 265, 871, 1009
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 5 cut(s) 33, 284, 687, 796, 1028
CaiI CAGNNNCTG 2 cut(s) 752, 1027
CfoI GCGC 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 219
Csp6I GTAC 2 cut(s) 637, 661
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviAII CATG 9 cut(s) 189, 338, 353, 570, 778, 796, 971, 991, 1115
CviQI GTAC 2 cut(s) 637, 661
DdeI CTNAG 3 cut(s) 204, 548, 670
DpnI GATC 8 cut(s) 194, 243, 424, 540, 626, 756, 800, 1161
DpnII GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
DraIII CACNNNGTG 1 cut(s) 691
Eam1104I CTCTTC 3 cut(s) 348, 755, 1158
EarI CTCTTC 3 cut(s) 348, 755, 1158
EciI GGCGGA 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 906
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 2 cut(s) 779, 1049
Eco88I CYCGRG 1 cut(s) 839
Eco91I GGTNACC 2 cut(s) 383, 1086
EcoO65I GGTNACC 2 cut(s) 383, 1086
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 1 cut(s) 906
EcoT22I ATGCAT 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 9 cut(s) 192, 341, 356, 573, 781, 799, 974, 994, 1118
FalI AAGNNNNNCTT 2 cut(s) 859, 891
FaqI GGGAC 1 cut(s) 938
FatI CATG 9 cut(s) 188, 337, 352, 569, 777, 795, 970, 990, 1114
FbaI TGATCA 2 cut(s) 241, 538
Fnu4HI GCNGC 5 cut(s) 176, 318, 495, 951, 1008
FokI GGATG 4 cut(s) 104, 272, 858, 1016
Fsp4HI GCNGC 5 cut(s) 176, 318, 495, 951, 1008
FspBI CTAG 4 cut(s) 152, 411, 1148, 1256
FspI TGCGCA 1 cut(s) 64
GlaI GCGC 1 cut(s) 64
GluI GCNGC 5 cut(s) 176, 318, 495, 951, 1008
HaeIII GGCC 1 cut(s) 1020
HhaI GCGC 1 cut(s) 65
Hin1II CATG 9 cut(s) 192, 341, 356, 573, 781, 799, 974, 994, 1118
Hin6I GCGC 1 cut(s) 63
HinP1I GCGC 1 cut(s) 63
HinfI GANTC 3 cut(s) 4, 767, 852
HphI GGTGA 3 cut(s) 275, 377, 947
Hpy166II GTNNAC 2 cut(s) 282, 902
Hpy188I TCNGA 7 cut(s) 246, 610, 629, 708, 759, 1240, 1262
Hpy8I GTNNAC 2 cut(s) 282, 902
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 6 cut(s) 265, 430, 458, 775, 1121, 1257
HpyCH4III ACNGT 3 cut(s) 466, 691, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 700
HpyF3I CTNAG 3 cut(s) 204, 548, 670
Hsp92II CATG 9 cut(s) 192, 341, 356, 573, 781, 799, 974, 994, 1118
HspAI GCGC 1 cut(s) 63
Ksp22I TGATCA 2 cut(s) 241, 538
Kzo9I GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
LguI GCTCTTC 3 cut(s) 348, 755, 1158
LmnI GCTCC 2 cut(s) 42, 910
Lsp1109I GCAGC 5 cut(s) 187, 329, 506, 962, 994
LweI GCATC 4 cut(s) 241, 798, 937, 994
MaeI CTAG 4 cut(s) 152, 411, 1148, 1256
MaeIII GTNAC 5 cut(s) 383, 413, 683, 791, 1086
MalI GATC 8 cut(s) 194, 243, 424, 540, 626, 756, 800, 1161
MboI GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
MboII GAAGA 7 cut(s) 280, 335, 772, 838, 1042, 1079, 1145
MfeI CAATTG 1 cut(s) 237
MflI RGATCY 1 cut(s) 624
MluCI AATT 2 cut(s) 237, 721
MlyI GAGTC 2 cut(s) 761, 861
MmeI TCCRAC 1 cut(s) 922
MnlI CCTC 9 cut(s) 32, 44, 174, 210, 242, 543, 569, 978, 1189
Mph1103I ATGCAT 1 cut(s) 813
MseI TTAA 2 cut(s) 306, 1137
MslI CAYNNNNRTG 1 cut(s) 989
MspR9I CCNGG 1 cut(s) 803
MunI CAATTG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 2 cut(s) 326, 700
NdeII GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
NlaIII CATG 9 cut(s) 192, 341, 356, 573, 781, 799, 974, 994, 1118
NlaIV GGNNCC 1 cut(s) 626
NmuCI GTSAC 4 cut(s) 383, 413, 683, 791
NsbI TGCGCA 1 cut(s) 64
NsiI ATGCAT 1 cut(s) 813
NspI RCATGY 2 cut(s) 341, 994
PciI ACATGT 1 cut(s) 990
PciSI GCTCTTC 3 cut(s) 348, 755, 1158
PkrI GCNGC 5 cut(s) 177, 319, 496, 952, 1009
PleI GAGTC 2 cut(s) 761, 860
PpsI GAGTC 2 cut(s) 761, 860
PscI ACATGT 1 cut(s) 990
Psp6I CCWGG 1 cut(s) 801
PspEI GGTNACC 2 cut(s) 383, 1086
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 1 cut(s) 626
PspPI GGNCC 1 cut(s) 219
PstI CTGCAG 2 cut(s) 314, 1167
PstNI CAGNNNCTG 2 cut(s) 752, 1027
PsuI RGATCY 1 cut(s) 624
RsaI GTAC 2 cut(s) 638, 662
RsaNI GTAC 2 cut(s) 637, 661
RseI CAYNNNNRTG 1 cut(s) 989
SapI GCTCTTC 3 cut(s) 348, 755, 1158
SaqAI TTAA 2 cut(s) 306, 1137
SatI GCNGC 5 cut(s) 176, 318, 495, 951, 1008
Sau3AI GATC 8 cut(s) 192, 241, 422, 538, 624, 754, 798, 1159
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 2 cut(s) 761, 861
ScrFI CCNGG 1 cut(s) 803
SfaNI GCATC 4 cut(s) 241, 798, 937, 994
SfcI CTRYAG 3 cut(s) 310, 615, 1163
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 989
SmlI CTYRAG 2 cut(s) 104, 212
SmoI CTYRAG 2 cut(s) 104, 212
SpeI ACTAGT 1 cut(s) 1255
Sse9I AATT 2 cut(s) 237, 721
SsiI CCGC 2 cut(s) 520, 1010
SspMI CTAG 4 cut(s) 152, 411, 1148, 1256
StyD4I CCNGG 1 cut(s) 801
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 3 cut(s) 466, 691, 791
TaqI TCGA 2 cut(s) 468, 1173
TasI AATT 2 cut(s) 237, 721
TatI WGTACW 1 cut(s) 660
Tru1I TTAA 2 cut(s) 306, 1137
Tru9I TTAA 2 cut(s) 306, 1137
TscAI CASTG 5 cut(s) 33, 291, 694, 796, 1028
TseFI GTSAC 4 cut(s) 383, 413, 683, 791
TseI GCWGC 5 cut(s) 175, 317, 494, 950, 1007
Tsp45I GTSAC 4 cut(s) 383, 413, 683, 791
TspDTI ATGAA 3 cut(s) 794, 868, 1103
TspRI CASTG 5 cut(s) 33, 291, 694, 796, 1028
VpaK11BI GGWCC 1 cut(s) 219
XceI RCATGY 2 cut(s) 341, 994
XspI CTAG 4 cut(s) 152, 411, 1148, 1256
Zsp2I ATGCAT 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.