MD15G1118300.v1.1

Zeaxanthin epoxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012103
Physical Location & Seq
Reverse (-)
8499385 .. 8501185
1801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1118300.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGAGTCTGAAGAAAAAACCCAAGGCAGTAATTGTGGGAGGGAGCATAGCAGGGGTATCATGCGCCCACACGCTCATATCAGCAGGGTGGGATGTTTTGTTGGTGGAGAAATCATATGCTCCCCCAACTGGAAGCCCAACTGGGGCTGGACTTGGCCTTGACCCTCTCTCTCTCAGACTCATCCAGTCATGGATTCAAGACCCTGACCTTCTCCACAAGGCCACTCTGCCGCTTACAATTGATCAGAATGATGCAATTGATGGTGAGAATAAGGTGAAATGGACGCTAACTAGAGATGAGGATTTCAACTGCAGAGCAGCACATTGGGCTGACCTGCATGCCCTTCTGTACAATGCACTACCACCAAACCTGTTTCTTTGGGGTCACCATTTCCTATCTTTCTCCATTTCCAGTGACAAGTCTTCAGTTATAGTGAAGGCTTCATCCCATCAGACTAATGAAATCATCGAAATTGTCGGTGATTTGCTCGTTGCAGCGGATGGATGTCTCTCTTCCATCCGCCAAAGTTTTGTCCCTGACCATAAACTGAGGTATTCGGGTTACTGCGCATGGAGAGGGATGCTTGATTTTACTGGAAATGAGAGTTCAGAAACCTTAACCGGCATCCGAAAGGAATACCCTGAGCTTGGGAAATGCTTGTACTTTGGCTTAGGTTCTGGGACGCACACCGTGCTATACGAGCTTCTGAACCGAAGGCTGAATTGGATTTGGTATGTCCACCAACCGGAGCCTGATCTGAAGCATAACTCGATGACCATGAAAGCGAGCAGCGACATGATCCAGAGTATGCACAAAGAAGCAGAGAAAATGTGGCTTCCAGAGTTTGTGAGAGTGATCAAAGAAACAAAAGAGCCTTTCATCAATGCCATATACGACAGTGATCCTTTAGAACAAATCTTCTGGGACAAAGTTGTACTAGTTGGGGATGCAGCTCACCCCACAACTCCTCATGCCCTAAGAAGCACAAACATGTCGGTCCTAGATGCTGCAGTGCTAGGGCAATGCTTGAAGAAGTGGGGAGCAGAAGATTTGCAATCAGCTCTTGAAGAATATCAGTCGATTCGGTTACCAGTCGTGTCCAAACAAGTCCTGCACGCAAGGCGAATGGGTCGGATTAAACAGGGCCTTGTTCTTCCTGATAGACAGCTTTTCAACCCCGAGACTGCTAATCCAGAGGAGTGCCAACAGCTGCAGCAGAAATTCATGCCCTTTTTCTGGGATGTTCCTTCCATACTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_3 NAD_binding_8

Protein Analysis

421

Amino Acids

47.15

Weight (kDa)

6.11

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 9 - 176 3.2e-06 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11330 AT5G11330
fragaria_vesca FvH4_2g29790 FvH4_2g29790
malus_domestica MD08G1141800.v1.1 MD15G1118300.v1.1 MD15G1335000.v1.1
prunus_persica Prupe.1G471700_v2.0.a1
pyrus_communis pycom15g10650
rosa_chinensis RchiOBHm_Chr6g0298811
rosa_laevigata RLG00000011483
rosa_multiflora Rmu_co8348659.1_g000001
rosa_roxburghii Rroxscaffold_7G00169310
rosa_rugosa Rorug06G0283000
rosa_samantha Rh6AG394700 Rh6BG402600 Rh6CG408300 Rh6DG395100
rosa_wichuraiana Rw6G034530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 342
AciI CCGC 3 cut(s) 230, 497, 520
AclWI GGATC 2 cut(s) 793, 896
AcsI RAATTY 1 cut(s) 1220
AcuI CTGAAG 3 cut(s) 29, 408, 779
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 3 cut(s) 350, 662, 936
AfiI CCNNNNNNNGG 5 cut(s) 128, 142, 647, 745, 1236
AflIII ACRYGT 1 cut(s) 990
AgsI TTSAA 5 cut(s) 197, 307, 1030, 1067, 1174
AhlI ACTAGT 1 cut(s) 937
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AluBI AGCT 6 cut(s) 646, 703, 953, 1061, 1168, 1210
AluI AGCT 6 cut(s) 646, 703, 953, 1061, 1168, 1210
Alw26I GTCTC 2 cut(s) 512, 1175
AlwI GGATC 2 cut(s) 793, 896
Ama87I CYCGRG 1 cut(s) 1178
AoxI GGCC 3 cut(s) 154, 219, 1144
ApeKI GCWGC 7 cut(s) 317, 494, 789, 950, 1007, 1210, 1213
ApoI RAATTY 1 cut(s) 1220
AspLEI GCGC 2 cut(s) 65, 569
AspS9I GGNCC 2 cut(s) 997, 1144
AsuHPI GGTGA 5 cut(s) 275, 286, 377, 491, 947
AvaI CYCGRG 1 cut(s) 1178
AvaII GGWCC 1 cut(s) 997
BbsI GAAGAC 1 cut(s) 414
BbvI GCAGC 7 cut(s) 329, 506, 801, 962, 994, 1197, 1225
BccI CCATC 4 cut(s) 254, 456, 494, 524
BclI TGATCA 2 cut(s) 241, 855
BcoDI GTCTC 2 cut(s) 512, 1175
BcuI ACTAGT 1 cut(s) 937
BfaI CTAG 4 cut(s) 291, 938, 1001, 1016
BfmI CTRYAG 3 cut(s) 310, 1008, 1211
BfuAI ACCTGC 1 cut(s) 342
BisI GCNGC 8 cut(s) 230, 318, 495, 790, 951, 1008, 1211, 1214
BlsI GCNGC 8 cut(s) 231, 319, 496, 791, 952, 1009, 1212, 1215
Bme18I GGWCC 1 cut(s) 997
BmeT110I CYCGRG 1 cut(s) 1178
BmgT120I GGNCC 2 cut(s) 997, 1144
BmiI GGNNCC 1 cut(s) 750
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 5 cut(s) 241, 570, 633, 937, 994
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 1 cut(s) 414
Bpu10I CCTNAGC 2 cut(s) 642, 670
BsaJI CCNNGG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 745
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 142, 647, 745, 1236
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 6 cut(s) 133, 145, 184, 411, 598, 1091
Bse3DI GCAATG 1 cut(s) 1028
BseDI CCNNGG 1 cut(s) 21
BseLI CCNNNNNNNGG 5 cut(s) 128, 142, 647, 745, 1236
BseMI GCAATG 1 cut(s) 1028
BseMII CTCAG 3 cut(s) 187, 539, 633
BseNI ACTGG 6 cut(s) 133, 145, 184, 411, 598, 1091
BseRI GAGGAG 2 cut(s) 957, 1211
BseXI GCAGC 7 cut(s) 329, 506, 801, 962, 994, 1197, 1225
BsgI GTGCAG 1 cut(s) 1097
BshFI GGCC 3 cut(s) 156, 221, 1146
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 2 cut(s) 621, 746
BslFI GGGAC 3 cut(s) 518, 694, 938
BslI CCNNNNNNNGG 5 cut(s) 128, 142, 647, 745, 1236
BsmAI GTCTC 2 cut(s) 512, 1175
BsmFI GGGAC 3 cut(s) 518, 694, 938
BsnI GGCC 3 cut(s) 156, 221, 1146
BsoBI CYCGRG 1 cut(s) 1178
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 5 cut(s) 241, 754, 798, 855, 901
BspACI CCGC 3 cut(s) 230, 497, 520
BspANI GGCC 3 cut(s) 156, 221, 1146
BspCNI CTCAG 3 cut(s) 186, 540, 634
BspLI GGNNCC 1 cut(s) 750
BspMAI CTGCAG 3 cut(s) 314, 1012, 1215
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 2 cut(s) 793, 896
BsrDI GCAATG 1 cut(s) 1028
BsrFI RCCGGY 1 cut(s) 620
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 6 cut(s) 133, 145, 184, 411, 598, 1091
BssAI RCCGGY 1 cut(s) 620
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 5 cut(s) 241, 754, 798, 855, 901
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 691, 899
Bst6I CTCTTC 1 cut(s) 517
BstAPI GCANNNNNTGC 1 cut(s) 691
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 339, 787, 1116
BstDEI CTNAG 5 cut(s) 173, 548, 642, 670, 977
BstEII GGTNACC 2 cut(s) 383, 1086
BstHHI GCGC 2 cut(s) 65, 569
BstKTI GATC 5 cut(s) 244, 757, 801, 858, 904
BstMAI GTCTC 2 cut(s) 512, 1175
BstMBI GATC 5 cut(s) 241, 754, 798, 855, 901
BstMWI GCNNNNNNNGC 4 cut(s) 326, 691, 700, 1120
BstNSI RCATGY 2 cut(s) 341, 994
BstPI GGTNACC 2 cut(s) 383, 1086
BstSFI CTRYAG 3 cut(s) 310, 1008, 1211
BstV1I GCAGC 7 cut(s) 329, 506, 801, 962, 994, 1197, 1225
BstV2I GAAGAC 1 cut(s) 414
BsuRI GGCC 3 cut(s) 156, 221, 1146
BtsI GCAGTG 1 cut(s) 1017
BtsIMutI CAGTG 3 cut(s) 418, 904, 1017
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 3 cut(s) 339, 787, 1116
CfoI GCGC 2 cut(s) 65, 569
Cfr10I RCCGGY 1 cut(s) 620
Cfr13I GGNCC 2 cut(s) 997, 1144
CseI GACGC 2 cut(s) 292, 691
Csp6I GTAC 3 cut(s) 349, 661, 935
CviAII CATG 9 cut(s) 60, 189, 338, 570, 778, 796, 971, 991, 1225
CviQI GTAC 3 cut(s) 349, 661, 935
DdeI CTNAG 5 cut(s) 173, 548, 642, 670, 977
DpnI GATC 5 cut(s) 243, 756, 800, 857, 903
DpnII GATC 5 cut(s) 241, 754, 798, 855, 901
DraIII CACNNNGTG 1 cut(s) 691
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
EciI GGCGGA 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 997
Eco57I CTGAAG 3 cut(s) 29, 408, 779
Eco88I CYCGRG 1 cut(s) 1178
Eco91I GGTNACC 2 cut(s) 383, 1086
EcoO109I RGGNCCY 1 cut(s) 1144
EcoO65I GGTNACC 2 cut(s) 383, 1086
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 9 cut(s) 63, 192, 341, 573, 781, 799, 974, 994, 1228
FaqI GGGAC 3 cut(s) 518, 694, 938
FatI CATG 9 cut(s) 59, 188, 337, 569, 777, 795, 970, 990, 1224
FauNDI CATATG 1 cut(s) 115
FbaI TGATCA 2 cut(s) 241, 855
Fnu4HI GCNGC 8 cut(s) 230, 318, 495, 790, 951, 1008, 1211, 1214
Fsp4HI GCNGC 8 cut(s) 230, 318, 495, 790, 951, 1008, 1211, 1214
FspBI CTAG 4 cut(s) 291, 938, 1001, 1016
FspI TGCGCA 1 cut(s) 568
GlaI GCGC 2 cut(s) 64, 568
GluI GCNGC 8 cut(s) 230, 318, 495, 790, 951, 1008, 1211, 1214
HaeIII GGCC 3 cut(s) 156, 221, 1146
HapII CCGG 2 cut(s) 621, 746
HgaI GACGC 2 cut(s) 292, 691
HhaI GCGC 2 cut(s) 65, 569
Hin1II CATG 9 cut(s) 63, 192, 341, 573, 781, 799, 974, 994, 1228
Hin6I GCGC 2 cut(s) 63, 567
HinP1I GCGC 2 cut(s) 63, 567
HinfI GANTC 4 cut(s) 4, 177, 193, 1081
HpaII CCGG 2 cut(s) 621, 746
HphI GGTGA 5 cut(s) 275, 286, 377, 491, 947
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 9 cut(s) 9, 176, 246, 453, 610, 629, 708, 759, 1134
Hpy188III TCNNGA 6 cut(s) 197, 802, 839, 1064, 1157, 1193
Hpy8I GTNNAC 1 cut(s) 739
HpyAV CCTTC 5 cut(s) 218, 353, 430, 708, 1257
HpyCH4III ACNGT 2 cut(s) 691, 899
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 691, 700, 1120
HpyF3I CTNAG 5 cut(s) 173, 548, 642, 670, 977
Hsp92II CATG 9 cut(s) 63, 192, 341, 573, 781, 799, 974, 994, 1228
HspAI GCGC 2 cut(s) 63, 567
Ksp22I TGATCA 2 cut(s) 241, 855
Kzo9I GATC 5 cut(s) 241, 754, 798, 855, 901
LmnI GCTCC 4 cut(s) 42, 124, 748, 1040
Lsp1109I GCAGC 7 cut(s) 329, 506, 801, 962, 994, 1197, 1225
LweI GCATC 5 cut(s) 241, 570, 633, 937, 994
MaeI CTAG 4 cut(s) 291, 938, 1001, 1016
MaeIII GTNAC 4 cut(s) 383, 413, 560, 1086
MalI GATC 5 cut(s) 243, 756, 800, 857, 903
MboI GATC 5 cut(s) 241, 754, 798, 855, 901
MboII GAAGA 8 cut(s) 22, 414, 504, 910, 1042, 1058, 1079, 1145
MfeI CAATTG 2 cut(s) 237, 255
MluCI AATT 6 cut(s) 30, 237, 255, 471, 721, 1220
MlyI GAGTC 2 cut(s) 13, 171
MmeI TCCRAC 1 cut(s) 1112
MnlI CCTC 7 cut(s) 32, 174, 292, 543, 569, 978, 1189
MseI TTAA 3 cut(s) 617, 1137, 1261
MslI CAYNNNNRTG 1 cut(s) 989
MspA1I CMGCKG 2 cut(s) 497, 1210
MspI CCGG 2 cut(s) 621, 746
MunI CAATTG 2 cut(s) 237, 255
MwoI GCNNNNNNNGC 4 cut(s) 326, 691, 700, 1120
NdeI CATATG 1 cut(s) 115
NdeII GATC 5 cut(s) 241, 754, 798, 855, 901
NlaIII CATG 9 cut(s) 63, 192, 341, 573, 781, 799, 974, 994, 1228
NlaIV GGNNCC 1 cut(s) 750
NmuCI GTSAC 2 cut(s) 383, 413
NsbI TGCGCA 1 cut(s) 568
NspI RCATGY 2 cut(s) 341, 994
PaeI GCATGC 1 cut(s) 341
PciI ACATGT 1 cut(s) 990
PfeI GAWTC 2 cut(s) 193, 1081
PkrI GCNGC 8 cut(s) 231, 319, 496, 791, 952, 1009, 1212, 1215
PleI GAGTC 2 cut(s) 12, 171
PpsI GAGTC 2 cut(s) 12, 171
PscI ACATGT 1 cut(s) 990
PspEI GGTNACC 2 cut(s) 383, 1086
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 2 cut(s) 997, 1144
PstI CTGCAG 3 cut(s) 314, 1012, 1215
PvuII CAGCTG 1 cut(s) 1210
RsaI GTAC 3 cut(s) 350, 662, 936
RsaNI GTAC 3 cut(s) 349, 661, 935
RseI CAYNNNNRTG 1 cut(s) 989
SaqAI TTAA 3 cut(s) 617, 1137, 1261
SatI GCNGC 8 cut(s) 230, 318, 495, 790, 951, 1008, 1211, 1214
Sau3AI GATC 5 cut(s) 241, 754, 798, 855, 901
Sau96I GGNCC 2 cut(s) 997, 1144
SchI GAGTC 2 cut(s) 13, 171
SfaNI GCATC 5 cut(s) 241, 570, 633, 937, 994
SfcI CTRYAG 3 cut(s) 310, 1008, 1211
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 1 cut(s) 989
SpeI ACTAGT 1 cut(s) 937
SphI GCATGC 1 cut(s) 341
Sse9I AATT 6 cut(s) 30, 237, 255, 471, 721, 1220
SsiI CCGC 3 cut(s) 230, 497, 520
SspMI CTAG 4 cut(s) 291, 938, 1001, 1016
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 691, 899
TaqI TCGA 3 cut(s) 468, 770, 1079
TaqII GACCGA 1 cut(s) 985
TasI AATT 6 cut(s) 30, 237, 255, 471, 721, 1220
TatI WGTACW 3 cut(s) 348, 660, 934
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 193, 1081
Tru1I TTAA 3 cut(s) 617, 1137, 1261
Tru9I TTAA 3 cut(s) 617, 1137, 1261
TscAI CASTG 3 cut(s) 418, 904, 1017
TseFI GTSAC 2 cut(s) 383, 413
TseI GCWGC 7 cut(s) 317, 494, 789, 950, 1007, 1210, 1213
Tsp45I GTSAC 2 cut(s) 383, 413
TspDTI ATGAA 5 cut(s) 432, 474, 794, 868, 1213
TspRI CASTG 3 cut(s) 418, 904, 1017
VpaK11BI GGWCC 1 cut(s) 997
XapI RAATTY 1 cut(s) 1220
XceI RCATGY 2 cut(s) 341, 994
XspI CTAG 4 cut(s) 291, 938, 1001, 1016
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.