FvH4_2g31951

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008080
Physical Location & Seq
Forward (+)
24347097 .. 24348510
1414 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31951.t1

Sequence Viewer

Length: 807 bp
ATGGATAAAGATAGAGAGCAAGTGAAACAGACAGTGGAGAGTGAGGCCATGGCCACTGCACTTCTTCTACACACAACCCCGAACTTCCTCTCCCCAATTTCTCCAACCCGAATCCCCAGACTCCACCGCCGCCACCTCCACCGCTACTGCACCAAAAAACCCAAAATACCCTCGTCGTTCCGAAACGGCGGCCCGGCAGAAACAGAGTGCCCTGTTCCGCTGGAGCAGCAGCCCATAAACGAGTACCAGAACCTCTCCAGCTCCTTCCCCTTCTCGTGGGCCTCCGGCGACCTCGTGGAGTACTGCTCTCGCCTGATCGCCACCGGCGCCTCCTTCGCGCTCTTCGTGGGCCTCCCCGTCGCGTCCTTCGGCGTCTTCGGGGCCCAGTCGGAGCCGCTGAAGCAGTCTCTCTACGCTGTTTCCAGTGGCTTCCTGGTGGTCACTCTCGCCGTCGTGAGGATGTACCTCGGTTGGGCTTACGTCGGCAACCGCTTGCTTAGTGCCACCGTTGAATACGAAGAGACTGGGTGGTATGATGGTCAGATATGGGTCAAGACTGCTGAGGTTTTGGCGCGCGATCGCCTTCTTGGTTCATTTTCTGTGAAGCCGGTGTTGAGCAGATTGAAGGTAACACTGGTGACTCTTGCAGCATCAATACTTGCATGCGTTGTTGTCCTCCTTTACATCGATGGAGGCCAGATTCAAGAAGCAGAGAATCGGGTTATACGGGGAGTTTACAACGATGATTCTGCAAGATCGTTCGAGCCAGATGCATTTTGTGGTGGTGATCCTGCTCCTCCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

269

Amino Acids

29.5

Weight (kDa)

6.44

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 70 - 213 6.4e-54 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 326
AccII CGCG 4 cut(s) 338, 362, 574, 576
AciI CCGC 7 cut(s) 127, 130, 142, 189, 218, 395, 490
AclWI GGATC 1 cut(s) 782
AcoI YGGCCR 1 cut(s) 51
AcuI CTGAAG 1 cut(s) 419
AcyI GRCGYC 2 cut(s) 327, 372
AfaI GTAC 3 cut(s) 245, 302, 464
AfiI CCNNNNNNNGG 3 cut(s) 276, 456, 472
AgsI TTSAA 3 cut(s) 512, 625, 704
AjnI CCWGG 1 cut(s) 432
AloI GAACNNNNNNTCC 2 cut(s) 74, 106
AluBI AGCT 1 cut(s) 261
AluI AGCT 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 411, 515
AlwI GGATC 1 cut(s) 782
AoxI GGCC 7 cut(s) 45, 51, 190, 279, 349, 381, 694
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 3 cut(s) 226, 229, 647
AsiSI GCGATCGC 1 cut(s) 580
AspLEI GCGC 4 cut(s) 329, 340, 574, 576
AspS9I GGNCC 5 cut(s) 191, 279, 349, 381, 382
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 649, 797
BaeGI GKGCMC 2 cut(s) 212, 385
BalI TGGCCA 1 cut(s) 53
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 385
BauI CACGAG 2 cut(s) 274, 293
BbsI GAAGAC 1 cut(s) 367
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 3 cut(s) 238, 241, 659
BccI CCATC 2 cut(s) 530, 683
BceAI ACGGC 2 cut(s) 202, 434
BcgI CGANNNNNNTGC 4 cut(s) 731, 752, 765, 786
BciT130I CCWGG 1 cut(s) 434
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 411, 515
BfoI RGCGCY 1 cut(s) 330
BisI GCNGC 6 cut(s) 130, 190, 227, 230, 395, 648
BlsI GCNGC 6 cut(s) 131, 191, 228, 231, 396, 649
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 2 cut(s) 194, 434
BmgT120I GGNCC 5 cut(s) 191, 279, 349, 381, 382
BmiI GGNNCC 4 cut(s) 328, 382, 383, 393
BmrFI CCNGG 2 cut(s) 194, 434
BmrI ACTGGG 2 cut(s) 379, 534
BmsI GCATC 2 cut(s) 659, 760
BmuI ACTGGG 2 cut(s) 379, 534
BpiI GAAGAC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 241, 242
Bpu10I CCTNAGC 1 cut(s) 561
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 687
BsaHI GRCGYC 2 cut(s) 327, 372
BsaJI CCNNGG 2 cut(s) 48, 466
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 456, 472
Bse118I RCCGGY 2 cut(s) 323, 607
Bse1I ACTGG 4 cut(s) 385, 423, 529, 639
BseBI CCWGG 1 cut(s) 434
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 2 cut(s) 48, 466
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 3 cut(s) 276, 456, 472
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 4 cut(s) 385, 423, 529, 639
BsePI GCGCGC 1 cut(s) 572
BseRI GAGGAG 1 cut(s) 786
BseSI GKGCMC 2 cut(s) 212, 385
BseXI GCAGC 3 cut(s) 238, 241, 659
BsgI GTGCAG 2 cut(s) 42, 133
Bsh1236I CGCG 4 cut(s) 338, 362, 574, 576
Bsh1285I CGRYCG 1 cut(s) 580
BshFI GGCC 7 cut(s) 47, 53, 192, 281, 351, 383, 696
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 1 cut(s) 687
BsiEI CGRYCG 1 cut(s) 580
BsiSI CCGG 4 cut(s) 194, 285, 324, 608
BslI CCNNNNNNNGG 3 cut(s) 276, 456, 472
BsmAI GTCTC 2 cut(s) 411, 515
BsnI GGCC 7 cut(s) 47, 53, 192, 281, 351, 383, 696
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 212, 385
Bsp143I GATC 4 cut(s) 315, 577, 755, 787
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 7 cut(s) 127, 130, 142, 189, 218, 395, 490
BspANI GGCC 7 cut(s) 47, 53, 192, 281, 351, 383, 696
BspCNI CTCAG 1 cut(s) 553
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 4 cut(s) 338, 362, 574, 576
BspLI GGNNCC 4 cut(s) 328, 382, 383, 393
BspPI GGATC 1 cut(s) 782
BspQI GCTCTTC 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 326
BsrFI RCCGGY 2 cut(s) 323, 607
BsrI ACTGG 4 cut(s) 385, 423, 529, 639
BssAI RCCGGY 2 cut(s) 323, 607
BssECI CCNNGG 2 cut(s) 48, 466
BssHII GCGCGC 1 cut(s) 572
BssMI GATC 4 cut(s) 315, 577, 755, 787
BssNI GRCGYC 2 cut(s) 327, 372
BssSI CACGAG 2 cut(s) 274, 293
BssT1I CCWWGG 1 cut(s) 48
Bst2BI CACGAG 2 cut(s) 274, 293
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 2 cut(s) 34, 508
Bst6I CTCTTC 2 cut(s) 347, 513
BstACI GRCGYC 2 cut(s) 327, 372
BstC8I GCNNGC 3 cut(s) 494, 574, 664
BstDEI CTNAG 2 cut(s) 497, 561
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 4 cut(s) 338, 362, 574, 576
BstH2I RGCGCY 1 cut(s) 330
BstHHI GCGC 4 cut(s) 329, 340, 574, 576
BstKTI GATC 4 cut(s) 318, 580, 758, 790
BstMAI GTCTC 2 cut(s) 411, 515
BstMBI GATC 4 cut(s) 315, 577, 755, 787
BstMCI CGRYCG 1 cut(s) 580
BstMWI GCNNNNNNNGC 4 cut(s) 226, 326, 335, 400
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 192, 432
BstSLI GKGCMC 2 cut(s) 212, 385
BstUI CGCG 4 cut(s) 338, 362, 574, 576
BstV1I GCAGC 3 cut(s) 238, 241, 659
BstV2I GAAGAC 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 687
BsuRI GGCC 7 cut(s) 47, 53, 192, 281, 351, 383, 696
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 1 cut(s) 465
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 4 cut(s) 39, 54, 430, 632
Cac8I GCNNGC 3 cut(s) 494, 574, 664
CfoI GCGC 4 cut(s) 329, 340, 574, 576
Cfr10I RCCGGY 2 cut(s) 323, 607
Cfr13I GGNCC 5 cut(s) 191, 279, 349, 381, 382
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 2 cut(s) 351, 361
Csp6I GTAC 3 cut(s) 244, 301, 463
CviAII CATG 2 cut(s) 49, 663
CviQI GTAC 3 cut(s) 244, 301, 463
DdeI CTNAG 2 cut(s) 497, 561
DinI GGCGCC 1 cut(s) 328
DpnI GATC 4 cut(s) 317, 579, 757, 789
DpnII GATC 4 cut(s) 315, 577, 755, 787
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 347, 513
EarI CTCTTC 2 cut(s) 347, 513
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 48
EcoT22I ATGCAT 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 328
EheI GGCGCC 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 2 cut(s) 52, 666
FaiI YATR 6 cut(s) 50, 236, 534, 547, 664, 725
FatI CATG 2 cut(s) 48, 662
Fnu4HI GCNGC 6 cut(s) 130, 190, 227, 230, 395, 648
FokI GGATG 1 cut(s) 472
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 6 cut(s) 130, 190, 227, 230, 395, 648
GlaI GCGC 4 cut(s) 328, 339, 573, 575
GluI GCNGC 6 cut(s) 130, 190, 227, 230, 395, 648
GsuI CTGGAG 2 cut(s) 241, 242
HaeII RGCGCY 1 cut(s) 330
HaeIII GGCC 7 cut(s) 47, 53, 192, 281, 351, 383, 696
HapII CCGG 4 cut(s) 194, 285, 324, 608
HgaI GACGC 2 cut(s) 351, 361
HhaI GCGC 4 cut(s) 329, 340, 574, 576
Hin1I GRCGYC 2 cut(s) 327, 372
Hin1II CATG 2 cut(s) 52, 666
Hin6I GCGC 4 cut(s) 327, 338, 572, 574
HinP1I GCGC 4 cut(s) 327, 338, 572, 574
HinfI GANTC 6 cut(s) 111, 120, 640, 700, 715, 746
HpaII CCGG 4 cut(s) 194, 285, 324, 608
HphI GGTGA 2 cut(s) 649, 797
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 3 cut(s) 182, 391, 543
Hpy188III TCNNGA 3 cut(s) 454, 553, 704
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 4 cut(s) 178, 362, 455, 485
HpyAV CCTTC 6 cut(s) 274, 280, 343, 376, 593, 619
HpyCH4III ACNGT 2 cut(s) 34, 508
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 6 cut(s) 59, 150, 647, 662, 752, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 226, 326, 335, 400
HpyF3I CTNAG 2 cut(s) 497, 561
HpySE526I ACGT 1 cut(s) 480
Hsp92I GRCGYC 2 cut(s) 327, 372
Hsp92II CATG 2 cut(s) 52, 666
HspAI GCGC 4 cut(s) 327, 338, 572, 574
KasI GGCGCC 1 cut(s) 326
Kzo9I GATC 4 cut(s) 315, 577, 755, 787
LguI GCTCTTC 1 cut(s) 347
LmnI GCTCC 4 cut(s) 223, 266, 391, 799
Lsp1109I GCAGC 3 cut(s) 238, 241, 659
LweI GCATC 2 cut(s) 659, 760
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 439, 628, 637
MalI GATC 4 cut(s) 317, 579, 757, 789
MboI GATC 4 cut(s) 315, 577, 755, 787
MboII GAAGA 4 cut(s) 56, 334, 367, 530
MhlI GDGCHC 2 cut(s) 212, 385
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 53
Mly113I GGCGCC 1 cut(s) 327
MlyI GAGTC 2 cut(s) 114, 634
MmeI TCCRAC 2 cut(s) 128, 369
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 775
MscI TGGCCA 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 53
MspA1I CMGCKG 2 cut(s) 220, 397
MspI CCGG 4 cut(s) 194, 285, 324, 608
MspR9I CCNGG 2 cut(s) 194, 434
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 4 cut(s) 338, 362, 574, 576
MwoI GCNNNNNNNGC 4 cut(s) 226, 326, 335, 400
NarI GGCGCC 1 cut(s) 327
NciI CCSGG 1 cut(s) 194
NcoI CCATGG 1 cut(s) 48
NdeII GATC 4 cut(s) 315, 577, 755, 787
NlaIII CATG 2 cut(s) 52, 666
NlaIV GGNNCC 4 cut(s) 328, 382, 383, 393
NmuCI GTSAC 2 cut(s) 439, 637
NsiI ATGCAT 1 cut(s) 775
NspI RCATGY 1 cut(s) 666
PaeI GCATGC 1 cut(s) 666
PauI GCGCGC 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PfeI GAWTC 4 cut(s) 111, 700, 715, 746
PkrI GCNGC 6 cut(s) 131, 191, 228, 231, 396, 649
Ple19I CGATCG 1 cut(s) 580
PleI GAGTC 2 cut(s) 114, 634
PluTI GGCGCC 1 cut(s) 330
PpsI GAGTC 2 cut(s) 114, 634
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 4 cut(s) 328, 382, 383, 393
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 5 cut(s) 191, 279, 349, 381, 382
PteI GCGCGC 1 cut(s) 572
PvuI CGATCG 1 cut(s) 580
RgaI GCGATCGC 1 cut(s) 580
RsaI GTAC 3 cut(s) 245, 302, 464
RsaNI GTAC 3 cut(s) 244, 301, 463
SapI GCTCTTC 1 cut(s) 347
SatI GCNGC 6 cut(s) 130, 190, 227, 230, 395, 648
Sau3AI GATC 4 cut(s) 315, 577, 755, 787
Sau96I GGNCC 5 cut(s) 191, 279, 349, 381, 382
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 2 cut(s) 114, 634
ScrFI CCNGG 2 cut(s) 194, 434
SduI GDGCHC 2 cut(s) 212, 385
SetI ASST 8 cut(s) 138, 255, 263, 294, 468, 483, 567, 630
SfaAI GCGATCGC 1 cut(s) 580
SfaNI GCATC 2 cut(s) 659, 760
SfoI GGCGCC 1 cut(s) 328
SgfI GCGATCGC 1 cut(s) 580
SgrAI CRCCGGYG 1 cut(s) 323
SphI GCATGC 1 cut(s) 666
Sse9I AATT 1 cut(s) 96
SsiI CCGC 7 cut(s) 127, 130, 142, 189, 218, 395, 490
SspDI GGCGCC 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 192, 432
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 34, 508
TaiI ACGT 1 cut(s) 483
TaqI TCGA 2 cut(s) 687, 762
TasI AATT 1 cut(s) 96
TatI WGTACW 1 cut(s) 300
TauI GCSGC 3 cut(s) 132, 192, 397
TfiI GAWTC 4 cut(s) 111, 700, 715, 746
TscAI CASTG 4 cut(s) 39, 61, 430, 639
TseFI GTSAC 2 cut(s) 439, 637
TseI GCWGC 3 cut(s) 226, 229, 647
Tsp45I GTSAC 2 cut(s) 439, 637
TspDTI ATGAA 1 cut(s) 582
TspRI CASTG 4 cut(s) 39, 61, 430, 639
XceI RCATGY 1 cut(s) 666
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
ZrmI AGTACT 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.