Rh6CG513500

Protein of unknown function (DUF1230)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0008080
Physical Location & Seq
Reverse (-)
67203452 .. 67204565
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG513500.1

Sequence Viewer

Length: 756 bp
ATGGCCACCGCACTTCTTCTACACCCAACCCCAAATTTCCTCTCACCAATCCCTCCAACCCCAAATCCCAGACTTCACCACTACCGCACCAAAATACCCTTCTTGTCGTCGTCGTCGTCGTGGTCGTTCCGAAACGGCGGCCCAGCAGAAACAGAGTGCCCTGTTCCGCTGGAGCAGCAGCCCATAAACGAGTACCAGAACCTCTCCACCTCCTTCCCCTTCTCGTGGGCCTCCGGCGACCTCGTCGAGTACTGCTCTCGCCTGATCGCCACCGGCGCCTCCTTCGCGCTCTTCGTGGGCCTCCCCGTCGCCTCCTTCGGCGTCTTCGGGGCCCAATCGGAGCCGCTCAAGCAGTCCCTCTACGCAGTTTCCAGTGGGTTTTTGGTGGTCACTCTCGCCGTGGTGAGGATGTACCTCGGTTGGGCTTACGTCGGCAACCGCTTGCTTAGTGCCACTGTTGAATACGAAGAGACTGGGTGGTATGATGGTCAGATATGGGTTAAGACTGCTGAAGTTTTGGCGCGTGATCGGCTTCTCGGTTCATTTTCTGTGAAGCCTGTGCTGAGCAGATTGAAGTTAACGCTGGTGACTCTTGCAGCATCGATACTTGCATGTGTGCTCGTCCTTCTTTACATTGATGGAGGCCAAATCCAAGAAGCCGAGAATCGCGTTATACGTGGAGTTTACAACGATGATTCTGCAAGATCGTTTGAGCCCGATGCGTTTTGTGGTGGTGATCCTGGTCCTCCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1230

Protein Analysis

251

Amino Acids

27.38

Weight (kDa)

5.4

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CGLD27-like PF06799 53 - 196 7.3e-54 Conserved in the green lineage and diatoms 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 275
AccBSI CCGCTC 1 cut(s) 346
AccII CGCG 3 cut(s) 287, 523, 669
AciI CCGC 6 cut(s) 9, 85, 138, 167, 344, 439
AclWI GGATC 1 cut(s) 731
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 34
AcuI CTGAAG 1 cut(s) 531
AcyI GRCGYC 2 cut(s) 276, 321
AfaI GTAC 3 cut(s) 194, 251, 413
AfiI CCNNNNNNNGG 3 cut(s) 225, 405, 421
AgsI TTSAA 2 cut(s) 461, 574
AjnI CCWGG 1 cut(s) 739
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 1 cut(s) 731
AoxI GGCC 6 cut(s) 3, 139, 228, 298, 330, 643
ApaI GGGCCC 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 175, 178, 596
ApoI RAATTY 1 cut(s) 34
AspLEI GCGC 3 cut(s) 278, 289, 523
AspS9I GGNCC 6 cut(s) 140, 228, 298, 330, 331, 743
AsuHPI GGTGA 5 cut(s) 36, 68, 415, 598, 746
AvaII GGWCC 1 cut(s) 743
BaeGI GKGCMC 2 cut(s) 161, 334
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 334, 717
BauI CACGAG 1 cut(s) 223
BbsI GAAGAC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 3 cut(s) 187, 190, 608
BccI CCATC 2 cut(s) 479, 632
BceAI ACGGC 2 cut(s) 151, 383
BcgI CGANNNNNNTGC 2 cut(s) 680, 714
BciT130I CCWGG 1 cut(s) 741
BcoDI GTCTC 1 cut(s) 464
BfoI RGCGCY 1 cut(s) 279
BisI GCNGC 5 cut(s) 139, 176, 179, 344, 597
BlpI GCTNAGC 1 cut(s) 563
BlsI GCNGC 5 cut(s) 140, 177, 180, 345, 598
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 741
Bme18I GGWCC 1 cut(s) 743
BmgT120I GGNCC 6 cut(s) 140, 228, 298, 330, 331, 743
BmiI GGNNCC 4 cut(s) 277, 331, 332, 342
BmrFI CCNGG 1 cut(s) 741
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 608, 709
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 316
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 332
Bsa29I ATCGAT 1 cut(s) 602
BsaAI YACGTR 1 cut(s) 677
BsaHI GRCGYC 2 cut(s) 276, 321
BsaJI CCNNGG 2 cut(s) 399, 415
Bsc4I CCNNNNNNNGG 3 cut(s) 225, 405, 421
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 2 cut(s) 372, 478
BseBI CCWGG 1 cut(s) 741
BseCI ATCGAT 1 cut(s) 602
BseDI CCNNGG 2 cut(s) 399, 415
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 3 cut(s) 225, 405, 421
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 2 cut(s) 372, 478
BseSI GKGCMC 2 cut(s) 161, 334
BseXI GCAGC 3 cut(s) 187, 190, 608
BseYI CCCAGC 1 cut(s) 142
Bsh1236I CGCG 3 cut(s) 287, 523, 669
BshFI GGCC 6 cut(s) 5, 141, 230, 300, 332, 645
BshNI GGYRCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 2 cut(s) 234, 273
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 225, 405, 421
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 6 cut(s) 5, 141, 230, 300, 332, 645
Bsp120I GGGCCC 1 cut(s) 330
Bsp1286I GDGCHC 4 cut(s) 161, 334, 621, 717
Bsp143I GATC 4 cut(s) 264, 526, 704, 736
Bsp1720I GCTNAGC 1 cut(s) 563
BspACI CCGC 6 cut(s) 9, 85, 138, 167, 344, 439
BspANI GGCC 6 cut(s) 5, 141, 230, 300, 332, 645
BspCNI CTCAG 1 cut(s) 555
BspDI ATCGAT 1 cut(s) 602
BspFNI CGCG 3 cut(s) 287, 523, 669
BspLI GGNNCC 4 cut(s) 277, 331, 332, 342
BspPI GGATC 1 cut(s) 731
BspQI GCTCTTC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 275
BsrBI CCGCTC 1 cut(s) 346
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 2 cut(s) 372, 478
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 399, 415
BssMI GATC 4 cut(s) 264, 526, 704, 736
BssNI GRCGYC 2 cut(s) 276, 321
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 741
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 2 cut(s) 296, 462
BstACI GRCGYC 2 cut(s) 276, 321
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 446, 563
BstDSI CCRYGG 1 cut(s) 399
BstF5I GGATG 1 cut(s) 414
BstFNI CGCG 3 cut(s) 287, 523, 669
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 3 cut(s) 278, 289, 523
BstKTI GATC 4 cut(s) 267, 529, 707, 739
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 4 cut(s) 264, 526, 704, 736
BstMWI GCNNNNNNNGC 5 cut(s) 175, 275, 284, 349, 529
BstNI CCWGG 1 cut(s) 741
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 1 cut(s) 739
BstSLI GKGCMC 2 cut(s) 161, 334
BstUI CGCG 3 cut(s) 287, 523, 669
BstV1I GCAGC 3 cut(s) 187, 190, 608
BstV2I GAAGAC 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 602
BsuRI GGCC 6 cut(s) 5, 141, 230, 300, 332, 645
BsuTUI ATCGAT 1 cut(s) 602
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 414
BtsIMutI CAGTG 2 cut(s) 379, 453
Cac8I GCNNGC 1 cut(s) 443
CfoI GCGC 3 cut(s) 278, 289, 523
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 6 cut(s) 140, 228, 298, 330, 331, 743
ClaI ATCGAT 1 cut(s) 602
CseI GACGC 1 cut(s) 310
Csp6I GTAC 3 cut(s) 193, 250, 412
CviAII CATG 1 cut(s) 612
CviQI GTAC 3 cut(s) 193, 250, 412
DdeI CTNAG 2 cut(s) 446, 563
DinI GGCGCC 1 cut(s) 277
DpnI GATC 4 cut(s) 266, 528, 706, 738
DpnII GATC 4 cut(s) 264, 526, 704, 736
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 296, 462
EarI CTCTTC 2 cut(s) 296, 462
Eco24I GRGCYC 2 cut(s) 334, 717
Eco47I GGWCC 1 cut(s) 743
Eco57I CTGAAG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 739
EcoT38I GRGCYC 2 cut(s) 334, 717
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
FaeI CATG 1 cut(s) 615
FaiI YATR 5 cut(s) 185, 483, 496, 613, 674
FaqI GGGAC 1 cut(s) 340
FatI CATG 1 cut(s) 611
Fnu4HI GCNGC 5 cut(s) 139, 176, 179, 344, 597
FokI GGATG 1 cut(s) 421
FriOI GRGCYC 2 cut(s) 334, 717
Fsp4HI GCNGC 5 cut(s) 139, 176, 179, 344, 597
GlaI GCGC 3 cut(s) 277, 288, 522
GluI GCNGC 5 cut(s) 139, 176, 179, 344, 597
GsaI CCCAGC 1 cut(s) 146
GsuI CTGGAG 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 6 cut(s) 5, 141, 230, 300, 332, 645
HapII CCGG 2 cut(s) 234, 273
HgaI GACGC 1 cut(s) 310
HhaI GCGC 3 cut(s) 278, 289, 523
Hin1I GRCGYC 2 cut(s) 276, 321
Hin1II CATG 1 cut(s) 615
Hin6I GCGC 3 cut(s) 276, 287, 521
HinP1I GCGC 3 cut(s) 276, 287, 521
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HinfI GANTC 3 cut(s) 589, 664, 695
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 2 cut(s) 234, 273
HphI GGTGA 5 cut(s) 36, 68, 415, 598, 746
Hpy166II GTNNAC 2 cut(s) 579, 685
Hpy188I TCNGA 3 cut(s) 131, 340, 492
Hpy8I GTNNAC 2 cut(s) 579, 685
Hpy99I CGWCG 7 cut(s) 112, 115, 118, 121, 248, 311, 434
HpyAV CCTTC 6 cut(s) 109, 223, 229, 292, 325, 635
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 2 cut(s) 429, 676
HpyCH4V TGCA 3 cut(s) 596, 611, 701
HpyF10VI GCNNNNNNNGC 5 cut(s) 175, 275, 284, 349, 529
HpyF3I CTNAG 2 cut(s) 446, 563
HpySE526I ACGT 2 cut(s) 429, 676
Hsp92I GRCGYC 2 cut(s) 276, 321
Hsp92II CATG 1 cut(s) 615
HspAI GCGC 3 cut(s) 276, 287, 521
KasI GGCGCC 1 cut(s) 275
KspAI GTTAAC 1 cut(s) 579
Kzo9I GATC 4 cut(s) 264, 526, 704, 736
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 2 cut(s) 172, 340
Lsp1109I GCAGC 3 cut(s) 187, 190, 608
LweI GCATC 2 cut(s) 608, 709
MaeII ACGT 2 cut(s) 429, 676
MaeIII GTNAC 2 cut(s) 388, 586
MalI GATC 4 cut(s) 266, 528, 706, 738
MbiI CCGCTC 1 cut(s) 346
MboI GATC 4 cut(s) 264, 526, 704, 736
MboII GAAGA 4 cut(s) 8, 283, 316, 479
MhlI GDGCHC 4 cut(s) 161, 334, 621, 717
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 34
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 1 cut(s) 583
MmeI TCCRAC 1 cut(s) 80
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 501, 578
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 2 cut(s) 234, 273
MspR9I CCNGG 1 cut(s) 741
MvaI CCWGG 1 cut(s) 741
MvnI CGCG 3 cut(s) 287, 523, 669
MwoI GCNNNNNNNGC 5 cut(s) 175, 275, 284, 349, 529
NarI GGCGCC 1 cut(s) 276
NdeII GATC 4 cut(s) 264, 526, 704, 736
NlaIII CATG 1 cut(s) 615
NlaIV GGNNCC 4 cut(s) 277, 331, 332, 342
NmeAIII GCCGAG 1 cut(s) 685
NmuCI GTSAC 2 cut(s) 388, 586
NspI RCATGY 1 cut(s) 615
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 5 cut(s) 113, 116, 122, 291, 673
PfeI GAWTC 2 cut(s) 664, 695
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 5 cut(s) 140, 177, 180, 345, 598
PleI GAGTC 1 cut(s) 583
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 583
Ppu21I YACGTR 1 cut(s) 677
Psp6I CCWGG 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 739
PspN4I GGNNCC 4 cut(s) 277, 331, 332, 342
PspOMI GGGCCC 1 cut(s) 330
PspPI GGNCC 6 cut(s) 140, 228, 298, 330, 331, 743
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 3 cut(s) 194, 251, 413
RsaNI GTAC 3 cut(s) 193, 250, 412
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 501, 578
SatI GCNGC 5 cut(s) 139, 176, 179, 344, 597
Sau3AI GATC 4 cut(s) 264, 526, 704, 736
Sau96I GGNCC 6 cut(s) 140, 228, 298, 330, 331, 743
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 1 cut(s) 583
ScrFI CCNGG 1 cut(s) 741
SduI GDGCHC 4 cut(s) 161, 334, 621, 717
SetI ASST 6 cut(s) 204, 212, 243, 417, 432, 679
SfaNI GCATC 2 cut(s) 608, 709
SfoI GGCGCC 1 cut(s) 277
SgrAI CRCCGGYG 1 cut(s) 272
SinI GGWCC 1 cut(s) 743
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 1 cut(s) 34
SsiI CCGC 6 cut(s) 9, 85, 138, 167, 344, 439
SspDI GGCGCC 1 cut(s) 275
StyD4I CCNGG 1 cut(s) 739
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 2 cut(s) 432, 679
TaqI TCGA 2 cut(s) 246, 602
TasI AATT 1 cut(s) 34
TatI WGTACW 1 cut(s) 249
TauI GCSGC 2 cut(s) 141, 346
TfiI GAWTC 2 cut(s) 664, 695
Tru1I TTAA 2 cut(s) 501, 578
Tru9I TTAA 2 cut(s) 501, 578
TscAI CASTG 2 cut(s) 379, 460
TseFI GTSAC 2 cut(s) 388, 586
TseI GCWGC 3 cut(s) 175, 178, 596
Tsp45I GTSAC 2 cut(s) 388, 586
TspDTI ATGAA 1 cut(s) 531
TspRI CASTG 2 cut(s) 379, 460
Tth111I GACNNNGTC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 743
XapI RAATTY 1 cut(s) 34
XceI RCATGY 1 cut(s) 615
XcmI CCANNNNNNNNNTGG 1 cut(s) 379
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.